Article ; Online: Computational correction of spatially variant optical aberrations in 3D single-molecule localization microscopy.
2019 Volume 27, Issue 9, Page(s) 12582–12599
Abstract: 3D single-molecule localization microscopy relies on fitting the shape of point-spread-functions (PSFs) recorded on a wide-field detector. However, optical aberrations distort those shapes, which compromises the accuracy and precision of single-molecule ... ...
Abstract | 3D single-molecule localization microscopy relies on fitting the shape of point-spread-functions (PSFs) recorded on a wide-field detector. However, optical aberrations distort those shapes, which compromises the accuracy and precision of single-molecule localization microscopy. Here, we employ a computational phase retrieval based on a vectorial PSF model to quantify the spatial variance of optical aberrations in a two-channel ultrawide-field single-molecule localization microscope. The use of a spatially variant PSF model enables accurate and precise emitter localization in x-, y- and z-directions throughout the entire field of view. |
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Language | English |
Publishing date | 2019-05-01 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 1491859-6 |
ISSN | 1094-4087 ; 1094-4087 |
ISSN (online) | 1094-4087 |
ISSN | 1094-4087 |
DOI | 10.1364/OE.27.012582 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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