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  1. Article ; Online: Computational correction of spatially variant optical aberrations in 3D single-molecule localization microscopy.

    Yan, T / Richardson, C J / Zhang, M / Gahlmann, A

    Optics express

    2019  Volume 27, Issue 9, Page(s) 12582–12599

    Abstract: 3D single-molecule localization microscopy relies on fitting the shape of point-spread-functions (PSFs) recorded on a wide-field detector. However, optical aberrations distort those shapes, which compromises the accuracy and precision of single-molecule ... ...

    Abstract 3D single-molecule localization microscopy relies on fitting the shape of point-spread-functions (PSFs) recorded on a wide-field detector. However, optical aberrations distort those shapes, which compromises the accuracy and precision of single-molecule localization microscopy. Here, we employ a computational phase retrieval based on a vectorial PSF model to quantify the spatial variance of optical aberrations in a two-channel ultrawide-field single-molecule localization microscope. The use of a spatially variant PSF model enables accurate and precise emitter localization in x-, y- and z-directions throughout the entire field of view.
    Language English
    Publishing date 2019-05-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1491859-6
    ISSN 1094-4087 ; 1094-4087
    ISSN (online) 1094-4087
    ISSN 1094-4087
    DOI 10.1364/OE.27.012582
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Phosphorous removal improvements and cost reductions leveraging cationic polymers and anionic polyacrylamides in Chemically Enhanced Treatment Wetland (CETW) systems

    Bachand, P.A.M / Richardson, C.J / Vaithiyanathan, P

    Ecological engineering. 2020 Mar. 01, v. 146

    2020  

    Abstract: Many wetland systems worldwide struggle with high nutrient influx from urban and agricultural inputs that often disturbs ecosystem balance. In the Florida Everglades, concentrations above 15 μg P L−1 have been shown to affect ecosystem balance and have ... ...

    Abstract Many wetland systems worldwide struggle with high nutrient influx from urban and agricultural inputs that often disturbs ecosystem balance. In the Florida Everglades, concentrations above 15 μg P L−1 have been shown to affect ecosystem balance and have long term effects on downstream periphyton, detritus, soil and macrophyte. Current Stormwater Treatment Areas (STAs) have been shown to effectively lower P levels on average to 41 μg P L−1 (SD of 31 μg P L−1). These levels, released into downstream Water Conservation Areas, exceed a long-term goal of 10 μg L−1 total P targeted for STA outflow concentrations. Here, we look at enhancing P removal through chemical addition to treatment wetland systems through a series of in-situ mesocosm studies informed through laboratory jar tests. In-situ mesocosm results showed Fe and Al metal-based coagulants lowered filtered total phosphorus (FTP) levels but they did not create settleable flocs, thus resulting in no change or even higher unfiltered total phosphorus (UTP) levels when compared to non-dosed control mesocosms. Proprietary cationic polymer blends and anionic polyacrylamide (PAM) added post coagulant dosing were tested to enhance P-removal performance. Cationic polymer addition significantly lowered FTP when combined with Al-based coagulants, suggesting enhanced charge neutralization, and improved UTP removal when combined with Fe-based coagulants, signifying improved bridging or electrostatic patch formation to create larger/denser floc. The addition of PAM after coagulant dosing generally did not improve FTP removal but did improve UTP removal when dosed at levels >0.5 mg L−1. For Fe-based systems, doubling PAM dosage (from 0.5 to 1.0 mg L−1) was slightly more beneficial for UTP removal than doubling coagulant blend dosage. For Al-based systems, doubling coagulant blend dosage was slightly more effective for UTP removal than doubling PAM dosage, but the difference was not significant. The chemical cost savings from utilizing PAMS were large: doubling coagulant blend dose increased chemical costs by $28,000–$100,000 per 1B L of water treated depending upon blend, while doubling PAM dose marginally increased chemical costs by $1700. Wetlands enhanced with chemical addition achieved levels as low as 20–25 μg L−1 UTP and <10 μg L−1 FTP under field conditions. These were lower than average achievable levels by non-dosed mesocosms (84 μg L−1 UTP and 43 μg L−1 FTP). While the goal of 10 μg L−1 total P was not met by these chemically enhanced treatment wetland (CETW) systems, strategic placement to treat water prior to entrance into STAs can potentially help STAs meet this criterion by extending the STAs' effectiveness and lifespan. More importantly, these systems can help mitigate downstream ecosystem imbalance and combat the formation and migration of a moving P front.
    Keywords aluminum ; coagulants ; conservation areas ; cost effectiveness ; detritus ; ecosystems ; iron ; long term effects ; macrophytes ; neutralization ; periphyton ; phosphorus ; polyacrylamide ; soil ; stormwater management ; total phosphorus ; water conservation ; wetlands ; Florida
    Language English
    Dates of publication 2020-0301
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1127407-4
    ISSN 0925-8574
    ISSN 0925-8574
    DOI 10.1016/j.ecoleng.2020.105722
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Erratum to: The Effects of Organic Matter Amendments on Greenhouse Gas Emissions from a Mitigation Wetland in Virginia's Coastal Plain

    Winton, R. S. / Richardson, C. J.

    Wetlands

    2015  Volume 35, Issue 5, Page(s) 981

    Language English
    Document type Article
    ZDB-ID 1097341-2
    ISSN 0277-5212
    Database Current Contents Nutrition, Environment, Agriculture

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  4. Article: The Effects of Organic Matter Amendments on Greenhouse Gas Emissions from a Mitigation Wetland in Virginia's Coastal Plain

    Winton, R. S. / Richardson, C. J.

    Wetlands

    2015  Volume 35, Issue 5, Page(s) 969

    Language English
    Document type Article
    ZDB-ID 1097341-2
    ISSN 0277-5212
    Database Current Contents Nutrition, Environment, Agriculture

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  5. Article: Riparian Habitat Dissimilarities in Restored and Reference Streams are Associated with Differences in Turtle Communities in the Southeastern Piedmont

    Dudley, M. P. / Ho, M. / Richardson, C. J.

    Wetlands

    2015  Volume 35, Issue 1, Page(s) 147

    Language English
    Document type Article
    ZDB-ID 1097341-2
    ISSN 0277-5212
    Database Current Contents Nutrition, Environment, Agriculture

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  6. Article: CaCO3 causes underestimation of NaOH extractable phosphorus in sequential fractionations

    Benzing, P / Richardson, C.J

    Soil science. 2005 Oct., v. 170, no. 10

    2005  

    Keywords soil nutrients ; phosphorus ; soil analysis ; extraction ; sodium hydroxide ; calcium carbonate ; fractionation ; phosphates ; soil mineralogy ; Histosols
    Language English
    Dates of publication 2005-10
    Size p. 802-809.
    Document type Article
    ZDB-ID 204569-2
    ISSN 0038-075X
    ISSN 0038-075X
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Different plant traits affect two pathways of riparian nitrogen removal in a restored freshwater wetland

    Sutton-Grier, A. E / Wright, J. P / Richardson, C. J

    Plant and soil. 2013 Apr., v. 365, no. 1-2

    2013  

    Abstract: BACKGROUND & AIMS: Plants may have dissimilar effects on ecosystem processes because they possess different attributes. Given increasing biodiversity losses, it is important to understand which plant traits are key drivers of ecosystem functions. To ... ...

    Abstract BACKGROUND & AIMS: Plants may have dissimilar effects on ecosystem processes because they possess different attributes. Given increasing biodiversity losses, it is important to understand which plant traits are key drivers of ecosystem functions. To address this question, we studied the response of two ecosystem functions that remove nitrogen (N) from wetland soils, the accumulation of N in plant biomass and denitrification potential (DNP), to variation in plant trait composition. METHODS: Our experiment manipulated plant composition in a riparian wetland. We determined relative importance of plant traits and environmental variables as predictors of each ecosystem function. RESULTS: We demonstrate that Water Use Efficiency (WUE) had a strong negative effect on biomass N. Root porosity and belowground biomass were negatively correlated with DNP. Trait ordination indicated that WUE was largely orthogonal to traits that maximized DNP. CONCLUSIONS: These results indicate that plant species with different trait values are required to maintain multiple ecosystem functions, and provide a more mechanistic, trait-based link between the recent findings that higher biodiversity is necessary for multi-functionality. While we selected plant traits based on ecological theory, several of the plant traits were not good predictors of each ecosystem function suggesting the ecological theory linking traits to function is incomplete and requires strengthening.
    Keywords belowground biomass ; biodiversity ; correlation ; ecosystems ; environmental factors ; nitrogen ; water use efficiency ; wetland soils ; wetlands
    Language English
    Dates of publication 2013-04
    Size p. 41-57.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 208908-7
    ISSN 1573-5036 ; 0032-079X
    ISSN (online) 1573-5036
    ISSN 0032-079X
    DOI 10.1007/s11104-011-1113-3
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: CaCO3 causes underestimation of NaOH extractable phosphorus in sequential fractionations

    Benzing, P / Richardson, C. J

    Soil science. 2005 Oct., v. 170, no. 10

    2005  

    Abstract: Sequential fractionation procedures that use an NaOH extraction for removal of P associated with iron and aluminum minerals, followed by an HCl extraction to dissolve P associated with calcium minerals, have been used extensively in investigating the ... ...

    Abstract Sequential fractionation procedures that use an NaOH extraction for removal of P associated with iron and aluminum minerals, followed by an HCl extraction to dissolve P associated with calcium minerals, have been used extensively in investigating the chemical partitioning of phosphorus in soils. Due to the high pH conditions that occur during NaOH extraction, it is hypothesized that PO4 freed from association with Fe and Al minerals can resorb to, or precipitate as calcium minerals. This phosphate would subsequently be recovered in the acid extraction, resulting in overestimation of calcium bound P and underestimation of Fe and Al bound P. To test the hypothesis, Fe bound phosphate was subjected to sequential fractionation with and without CaCO3 present. Without CaCO3, 57% of the total recovered phosphorus was found in the 0.1N NaOH extraction, and 15% in the 0.5N HCl extraction. With CaCO3, 4% was found in the 0.1N NaOH extraction and 39% in the 0.5N HCl extraction. Fe-bound phosphate was added and then sequentially extracted from a typical Everglades Histosol soil. Mean recoveries of phosphate added as Fe-bound P (95% confidence intervals in parentheses) were estimated to be 33% (±2.4%) in HCl, 32% (±4.8%) as inorganic phosphate in NaOH, 24% (±13.6%) organic P in NaOH, 14% (±1.3%) in KCl, and 8% (±2.8%) in the residual fraction. These results show that the procedure underestimated the amount of iron- and aluminum-bound P and falsely attributed Fe-bound P to the Ca-bound P fraction.
    Keywords Histosols ; aluminum ; calcium ; calcium carbonate ; confidence interval ; fractionation ; hydrochloric acid ; iron ; minerals ; pH ; phosphates ; phosphorus ; potassium chloride ; sodium hydroxide
    Language English
    Dates of publication 2005-10
    Size p. 802-809.
    Publishing place Lippincott Williams & Wilkins
    Document type Article
    Note Fairleigh Dickinson University, 285 Madison Avenue, M-EC1-01, Madison, NJ 07940. Dr. Paul Benzing is corresponding author. E-mail: pbenzing@fdu.edu
    ZDB-ID 204569-2
    ISSN 0038-075X
    ISSN 0038-075X
    DOI 10.1097/01.ss.0000190501.98437.d1
    Database NAL-Catalogue (AGRICOLA)

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  9. Article: Coastal Freshwater Wetland Plant Community Response to Seasonal Drought and Flooding in Northwestern Costa Rica

    Osland, M. J. / Gonzalez, E. / Richardson, C. J.

    Wetlands

    2011  Volume 31, Issue 4, Page(s) 641

    Language English
    Document type Article
    ZDB-ID 1097341-2
    ISSN 0277-5212
    Database Current Contents Nutrition, Environment, Agriculture

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  10. Article: Identification of patients at highest risk for ventilator-associated pneumonia in the surgical intensive care unit.

    Richardson, C J / Rodriguez, J L

    American journal of surgery

    2000  Volume 179, Issue 2A Suppl, Page(s) 8S–11S

    Abstract: Ventilator-acquired pneumonia (VAP) is a major problem for patients admitted to the surgical intensive care unit mechanically ventilated. Recently, we have identified both clinical and immunologic factors associated with the development of VAP. The ... ...

    Abstract Ventilator-acquired pneumonia (VAP) is a major problem for patients admitted to the surgical intensive care unit mechanically ventilated. Recently, we have identified both clinical and immunologic factors associated with the development of VAP. The clinical risk factors are associated with the severity of the injury and the length of mechanical ventilation. The immunologic risk factors are associated with the local lung inflammatory response that is unchecked and affects local cell function. The combination of the severity of injury, the length of mechanical ventilation, and the failure to "auto-regulate" the lung response places the host at risk of VAP. In the next millennium, if we are to make significant advances in the management of VAP, we will need to understand the pathophysiology of the disease process. Then we can develop preventive strategies that will reduce the morbidity and the associated cost of VAP.
    MeSH term(s) Age Factors ; Aged ; Humans ; Immunity, Cellular ; Intensive Care Units ; Pneumonia, Aspiration/etiology ; Pneumonia, Aspiration/immunology ; Pneumonia, Aspiration/prevention & control ; Respiration, Artificial/adverse effects ; Risk Assessment ; Severity of Illness Index ; Time Factors
    Language English
    Publishing date 2000-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2953-1
    ISSN 1879-1883 ; 0002-9610
    ISSN (online) 1879-1883
    ISSN 0002-9610
    Database MEDical Literature Analysis and Retrieval System OnLINE

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