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  1. Article: Validation of environmental DNA sampling for determination of Ceratonova shasta (Cnidaria: Myxozoa) distribution in Plumas National Forest, CA

    Richey, Christine A / Kenelty, Kirsten V / Hopkins, Kristina Van Stone / Stevens, Brittany N / Martínez-López, Beatriz / Hallett, Sascha L / Atkinson, Stephen D / Bartholomew, Jerri L / Soto, Esteban

    Parasitology research. 2020 Mar., v. 119, no. 3

    2020  

    Abstract: Ceratonova shasta is the etiological agent of myxozoan-associated enteronecrosis in North American salmonids. The parasite’s life cycle involves waterborne spores and requires both a salmonid fish and a freshwater fabriciid annelid. The success and ... ...

    Abstract Ceratonova shasta is the etiological agent of myxozoan-associated enteronecrosis in North American salmonids. The parasite’s life cycle involves waterborne spores and requires both a salmonid fish and a freshwater fabriciid annelid. The success and survival of annelids can be enhanced by flow moderation by dams, and through the erosion of fine sediments into stream channels following wildfires. In this study, the presence of C. shasta environmental/ex-host DNA (eDNA) in river water and substrate samples collected from areas affected by recent fire activity in California, USA, was investigated. Additionally, DNA loads in the environment were compared to C. shasta infection in sentinel-exposed rainbow trout (Oncorhynchus mykiss). Significant associations between C. shasta detection in environmental samples and location within a wildfire perimeter (p = 0.002), between C. shasta detection in sentinel fish and exposure location within a wildfire perimeter (p = 0.015), and between C. shasta detection in fish and locations where water temperature was above the median (p < 0.001) were observed. Additionally, a higher prevalence of C. shasta infection in fish was detected where C. shasta was also detected in environmental samples (p < 0.001). Results suggest that pathogen eDNA sampling can be used as a non-invasive, rapid, specific, and sensitive method for establishing risk of C. shasta infection in wild populations. Knowledge of the complete life cycle of the target parasite, including ecology of each host, can inform the choice of eDNA sampling strategy. Environmental DNA sampling also revealed a novel species of Ceratonova, not yet observed in a host.
    Keywords Annelida ; Ceratomyxa shasta ; Oncorhynchus mykiss ; environmental DNA ; fish ; fish diseases ; freshwater ; parasites ; pathogens ; risk ; river water ; sediments ; spores ; stream channels ; water temperature ; wildfires ; California ; Plumas National Forest
    Language English
    Dates of publication 2020-03
    Size p. 859-870.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-019-06509-1
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  2. Article ; Online: Validation of environmental DNA sampling for determination of Ceratonova shasta (Cnidaria: Myxozoa) distribution in Plumas National Forest, CA.

    Richey, Christine A / Kenelty, Kirsten V / Hopkins, Kristina Van Stone / Stevens, Brittany N / Martínez-López, Beatriz / Hallett, Sascha L / Atkinson, Stephen D / Bartholomew, Jerri L / Soto, Esteban

    Parasitology research

    2020  Volume 119, Issue 3, Page(s) 859–870

    Abstract: Ceratonova shasta is the etiological agent of myxozoan-associated enteronecrosis in North American salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and a freshwater fabriciid annelid. The success and ... ...

    Abstract Ceratonova shasta is the etiological agent of myxozoan-associated enteronecrosis in North American salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and a freshwater fabriciid annelid. The success and survival of annelids can be enhanced by flow moderation by dams, and through the erosion of fine sediments into stream channels following wildfires. In this study, the presence of C. shasta environmental/ex-host DNA (eDNA) in river water and substrate samples collected from areas affected by recent fire activity in California, USA, was investigated. Additionally, DNA loads in the environment were compared to C. shasta infection in sentinel-exposed rainbow trout (Oncorhynchus mykiss). Significant associations between C. shasta detection in environmental samples and location within a wildfire perimeter (p = 0.002), between C. shasta detection in sentinel fish and exposure location within a wildfire perimeter (p = 0.015), and between C. shasta detection in fish and locations where water temperature was above the median (p < 0.001) were observed. Additionally, a higher prevalence of C. shasta infection in fish was detected where C. shasta was also detected in environmental samples (p < 0.001). Results suggest that pathogen eDNA sampling can be used as a non-invasive, rapid, specific, and sensitive method for establishing risk of C. shasta infection in wild populations. Knowledge of the complete life cycle of the target parasite, including ecology of each host, can inform the choice of eDNA sampling strategy. Environmental DNA sampling also revealed a novel species of Ceratonova, not yet observed in a host.
    MeSH term(s) Animals ; California/epidemiology ; Environmental DNA/analysis ; Environmental DNA/genetics ; Environmental Monitoring/methods ; Fish Diseases/epidemiology ; Fish Diseases/parasitology ; Forests ; Fresh Water/chemistry ; Fresh Water/parasitology ; Myxozoa/classification ; Myxozoa/genetics ; Myxozoa/isolation & purification ; Oncorhynchus mykiss/parasitology ; Parasitic Diseases, Animal/epidemiology ; Parasitic Diseases, Animal/parasitology
    Chemical Substances Environmental DNA
    Language English
    Publishing date 2020-01-02
    Publishing country Germany
    Document type Journal Article ; Validation Study
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-019-06509-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uh II Zs.124: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Distribution and Prevalence of Myxobolus cerebralis in Postfire Areas of Plumas National Forest: Utility of Environmental DNA Sampling

    Richey, Christine A. / Kenelty, Kirsten V. / Van Stone Hopkins, Kristina / Stevens, Brittany N. / Martínez‐López, Beatriz / Barnum, Samantha M. / Hallett, Sascha L. / Atkinson, Stephen D. / Bartholomew, Jerri L. / Soto, Esteban

    Journal of aquatic animal health. 2018 June, v. 30, no. 2

    2018  

    Abstract: Myxobolus cerebralis is a myxozoan parasite and the etiological agent of whirling disease in salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and the benthic freshwater oligochaete worm Tubifex tubifex ( ... ...

    Abstract Myxobolus cerebralis is a myxozoan parasite and the etiological agent of whirling disease in salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and the benthic freshwater oligochaete worm Tubifex tubifex (Oligochaeta: Tubificidae). Wildfires can lead to the erosion of fine sediments into stream channels and have been implicated as promoting environmental conditions that are suitable for the survival and success of T. tubifex, whose presence in turn can affect the prevalence of M. cerebralis. Analysis of environmental DNA (eDNA) has the potential to be a powerful tool for evaluating the presence of microorganisms, for which direct observation is impossible. We investigated the presence of M. cerebraliseDNA in river water and river sediment samples collected from areas affected by recent fire activity in Plumas National Forest, California. We compared eDNA loads in the environment to M. cerebralis infection in T. tubifex and sentinel‐exposed Rainbow Trout Oncorhynchus mykiss and the presence of T. tubifex lineages in the same environment. For the latter, we developed a multiplex quantitative PCR assay for detection of T. tubifex lineages I, III, and V. Lineage IIIT. tubifex and M. cerebralis (eDNA as well as DNA extracted from fish and worm tissues) were detected only in samples obtained from areas affected by the Moonlight wildfire. The association between M. cerebralis infection in sentinel‐exposed fish and eDNA detection in environmental samples only approached significance at a P‐value of 0.056. However, given the difference in relative effort between the two sampling methods (host versus nonhost environment), our data suggest that eDNA sampling of water and substrate is a promising approach for surveillance of myxozoan fish parasites.
    Keywords Myxobolus cerebralis ; Oncorhynchus mykiss ; Tubifex tubifex ; animal health ; environmental DNA ; etiological agents ; fish ; freshwater ; monitoring ; parasites ; quantitative polymerase chain reaction ; river water ; rivers ; sediments ; wildfires ; California ; Plumas National Forest
    Language English
    Dates of publication 2018-06
    Size p. 130-143.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 1019919-6
    ISSN 1548-8667 ; 0899-7659
    ISSN (online) 1548-8667
    ISSN 0899-7659
    DOI 10.1002/aah.10014
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 5030: Show issues

    Order via subito

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  4. Article ; Online: Distribution and Prevalence of Myxobolus cerebralis in Postfire Areas of Plumas National Forest: Utility of Environmental DNA Sampling.

    Richey, Christine A / Kenelty, Kirsten V / Van Stone Hopkins, Kristina / Stevens, Brittany N / Martínez-López, Beatriz / Barnum, Samantha M / Hallett, Sascha L / Atkinson, Stephen D / Bartholomew, Jerri L / Soto, Esteban

    Journal of aquatic animal health

    2018  Volume 30, Issue 2, Page(s) 130–143

    Abstract: Myxobolus cerebralis is a myxozoan parasite and the etiological agent of whirling disease in salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and the benthic freshwater oligochaete worm Tubifex tubifex ( ... ...

    Abstract Myxobolus cerebralis is a myxozoan parasite and the etiological agent of whirling disease in salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and the benthic freshwater oligochaete worm Tubifex tubifex (Oligochaeta: Tubificidae). Wildfires can lead to the erosion of fine sediments into stream channels and have been implicated as promoting environmental conditions that are suitable for the survival and success of T. tubifex, whose presence in turn can affect the prevalence of M. cerebralis. Analysis of environmental DNA (eDNA) has the potential to be a powerful tool for evaluating the presence of microorganisms, for which direct observation is impossible. We investigated the presence of M. cerebraliseDNA in river water and river sediment samples collected from areas affected by recent fire activity in Plumas National Forest, California. We compared eDNA loads in the environment to M. cerebralis infection in T. tubifex and sentinel-exposed Rainbow Trout Oncorhynchus mykiss and the presence of T. tubifex lineages in the same environment. For the latter, we developed a multiplex quantitative PCR assay for detection of T. tubifex lineages I, III, and V. Lineage IIIT. tubifex and M. cerebralis (eDNA as well as DNA extracted from fish and worm tissues) were detected only in samples obtained from areas affected by the Moonlight wildfire. The association between M. cerebralis infection in sentinel-exposed fish and eDNA detection in environmental samples only approached significance at a P-value of 0.056. However, given the difference in relative effort between the two sampling methods (host versus nonhost environment), our data suggest that eDNA sampling of water and substrate is a promising approach for surveillance of myxozoan fish parasites.
    MeSH term(s) Animals ; California ; DNA/analysis ; Ecosystem ; Environmental Monitoring ; Fish Diseases/parasitology ; Geologic Sediments/chemistry ; Multiplex Polymerase Chain Reaction/methods ; Multiplex Polymerase Chain Reaction/veterinary ; Myxobolus/genetics ; Myxobolus/isolation & purification ; Oligochaeta/genetics ; Oligochaeta/parasitology ; Oncorhynchus mykiss ; Parasitic Diseases, Animal/parasitology ; Rivers/chemistry ; Wildfires
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2018-04-30
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1019919-6
    ISSN 1548-8667 ; 0899-7659
    ISSN (online) 1548-8667
    ISSN 0899-7659
    DOI 10.1002/aah.10014
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 5030: Show issues

    Order via subito

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  5. Article ; Online: HYPERMUCOVISCOUS KLEBSIELLA PNEUMONIAE ISOLATES FROM STRANDED AND WILD-CAUGHT MARINE MAMMALS OF THE US PACIFIC COAST: PREVALENCE, PHENOTYPE, AND GENOTYPE.

    Whitaker, Dane M / Reichley, Stephen R / Griffin, Matt J / Prager, Katherine / Richey, Christine A / Kenelty, Kirsten V / Stevens, Brittany N / Lloyd-Smith, James O / Johnson, Christine K / Duignan, Padraig / Johnson, Shawn / Rios, Carlos / DeLong, Robert / Halaska, Barbie / Rust, Lauren / Byrne, Barbara A / Struve, Carsten / Barnum, Samantha / Soto, Esteban

    Journal of wildlife diseases

    2018  Volume 54, Issue 4, Page(s) 659–670

    Abstract: Emergent hypermucoviscous (HMV) strains of Klebsiella pneumoniae have been reported in multiple marine mammal species; however, there is limited information regarding the epidemiology and pathogenesis of this infection in these species. We determined the ...

    Abstract Emergent hypermucoviscous (HMV) strains of Klebsiella pneumoniae have been reported in multiple marine mammal species; however, there is limited information regarding the epidemiology and pathogenesis of this infection in these species. We determined the prevalence of HMV K. pneumoniae in wild-caught and stranded marine mammal populations on the US Pacific Coast. Samples were collected from 270 free-ranging California sea lions (CSLs; Zalophus californianus) captured at three discrete sampling sites and from 336 stranded marine mammals of various species. We recovered HMV K. pneumoniae only from CSLs, with a prevalence of 1.5% (4 of 275) in stranded animals, compared with 1.1% (3 of 270) in wild-caught animals. We assessed the phenotypic and genotypic variability of recovered HMV K. pneumoniae isolates recovered from CSLs ( n=11) and of archival HMV and non-HMV isolates from stranded marine mammals ( n=19). All but two HMV isolates were of the K2 serotype, whereas none of the non-HMV isolates belonged to this serotype. Of the HMV isolates, 96% (24 of 25) were PCR positive for the HMV-associated gene p- rmpA, whereas 92% (23 of 25) were PCR positive for p- rmpA2. Genetic fingerprinting by repetitive extragenic palindromic PCR showed four discrete clusters, demonstrating genotypic variability that loosely correlated with phenotype. Antimicrobial susceptibility testing revealed all isolates from stranded CSLs were susceptible to ceftiofur, indicating this antimicrobial agent is an appropriate choice for treatment of HMV K. pneumoniae infections in stranded CSLs. Our culture assay could reliably detect HMV K. pneumoniae from concentrations as low as 10
    MeSH term(s) Animals ; Animals, Wild ; California/epidemiology ; Female ; Klebsiella Infections/epidemiology ; Klebsiella Infections/microbiology ; Klebsiella Infections/veterinary ; Klebsiella pneumoniae/genetics ; Klebsiella pneumoniae/physiology ; Male ; Prevalence ; Sea Lions/microbiology ; Seals, Earless/microbiology
    Language English
    Publishing date 2018-05-07
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 410709-3
    ISSN 1943-3700 ; 0090-3558
    ISSN (online) 1943-3700
    ISSN 0090-3558
    DOI 10.7589/2017-07-178
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1441: Show issues Location:
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