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  1. Article ; Online: High-Resolution Large-Area Image Analysis Deciphers the Distribution of Salmonella Cells and ECM Components in Biofilms Formed on Charged PEDOT:PSS Surfaces.

    Ray, Sanhita / Löffler, Susanne / Richter-Dahlfors, Agneta

    Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    2024  , Page(s) e2307322

    Abstract: Biofilms, comprised of cells embedded in extracellular matrix (ECM), enable bacterial surface colonization and contribute to pathogenesis and biofouling. Yet, antibacterial surfaces are mainly evaluated for their effect on bacterial cells rather than the ...

    Abstract Biofilms, comprised of cells embedded in extracellular matrix (ECM), enable bacterial surface colonization and contribute to pathogenesis and biofouling. Yet, antibacterial surfaces are mainly evaluated for their effect on bacterial cells rather than the ECM. Here, a method is presented to separately quantify amounts and distribution of cells and ECM in Salmonella biofilms grown on electroactive poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS). Within a custom-designed biofilm reactor, biofilm forms on PEDOT:PSS surfaces electrically addressed with a bias potential and simultaneous recording of the resulting current. The amount and distribution of cells and ECM in biofilms are analyzed using a fluorescence-based spectroscopic mapping technique and fluorescence confocal microscopy combined with advanced image processing. The study shows that surface charge leads to upregulated ECM production, leaving the cell counts largely unaffected. An altered texture is also observed, with biofilms forming small foci or more continuous structures. Supported by mutants lacking ECM production, ECM is identified as an important target when developing antibacterial strategies. Also, a central role for biofilm distribution is highlighted that likely influences antimicrobial susceptibility in biofilms. This work provides yet a link between conductive polymer materials and bacterial metabolism and reveals for the first time a specific effect of electrochemical addressing on bacterial ECM formation.
    Language English
    Publishing date 2024-01-15
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2808093-2
    ISSN 2198-3844 ; 2198-3844
    ISSN (online) 2198-3844
    ISSN 2198-3844
    DOI 10.1002/advs.202307322
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Fluorescent optotracers for bacterial and biofilm detection and diagnostics.

    Richter-Dahlfors, Agneta / Kärkkäinen, Elina / Choong, Ferdinand X

    Science and technology of advanced materials

    2023  Volume 24, Issue 1, Page(s) 2246867

    Abstract: Effective treatment of bacterial infections requires methods that accurately and quickly identify which antibiotic should be prescribed. This review describes recent research on the development of optotracing methodologies for bacterial and biofilm ... ...

    Abstract Effective treatment of bacterial infections requires methods that accurately and quickly identify which antibiotic should be prescribed. This review describes recent research on the development of optotracing methodologies for bacterial and biofilm detection and diagnostics. Optotracers are small, chemically well-defined, anionic fluorescent tracer molecules that detect peptide- and carbohydrate-based biopolymers. This class of organic molecules (luminescent conjugated oligothiophenes) show unique electronic, electrochemical and optical properties originating from the conjugated structure of the compounds. The photophysical properties are further improved as donor-acceptor-donor (D-A-D)-type motifs are incorporated in the conjugated backbone. Optotracers bind their biopolymeric target molecules via electrostatic interactions. Binding alters the optical properties of these tracer molecules, shown as altered absorption and emission spectra, as well as ON-like switch of fluorescence. As the optotracer provides a defined spectral signature for each binding partner, a fingerprint is generated that can be used for identification of the target biopolymer. Alongside their use for
    Language English
    Publishing date 2023-09-05
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2027985-1
    ISSN 1878-5514 ; 1468-6996
    ISSN (online) 1878-5514
    ISSN 1468-6996
    DOI 10.1080/14686996.2023.2246867
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Bacterial Sensing and Biofilm Monitoring for Infection Diagnostics.

    Parlak, Onur / Richter-Dahlfors, Agneta

    Macromolecular bioscience

    2020  Volume 20, Issue 11, Page(s) e2000129

    Abstract: Recent insights into the rapidly emerging field of bacterial sensing and biofilm monitoring for infection diagnostics are discussed as well as recent key developments and emerging technologies in the field. Electrochemical sensing of bacteria and ... ...

    Abstract Recent insights into the rapidly emerging field of bacterial sensing and biofilm monitoring for infection diagnostics are discussed as well as recent key developments and emerging technologies in the field. Electrochemical sensing of bacteria and bacterial biofilm via synthetic, natural, and engineered recognition, as well as direct redox-sensing approaches via algorithm-based optical sensing, and tailor-made optotracing technology are discussed. These technologies are highlighted to answer the very critical question: "how can fast and accurate bacterial sensing and biofilm monitoring be achieved? Following on from that: "how can these different sensing concepts be translated for use in infection diagnostics? A central obstacle to this transformation is the absence of direct and fast analysis methods that provide high-throughput results and bio-interfaces that can control and regulate the means of communication between biological and electronic systems. Here, the overall progress made to date in building such translational efforts at the level of an individual bacterial cell to a bacterial community is discussed.
    MeSH term(s) Bacteria/metabolism ; Bacterial Infections/diagnosis ; Biofilms ; Biosensing Techniques/methods ; Electrochemistry ; Molecularly Imprinted Polymers/chemistry
    Chemical Substances Molecularly Imprinted Polymers
    Language English
    Publishing date 2020-06-26
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2039130-4
    ISSN 1616-5195 ; 1616-5187
    ISSN (online) 1616-5195
    ISSN 1616-5187
    DOI 10.1002/mabi.202000129
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A Cinematic View of Tissue Microbiology in the Live Infected Host.

    Richter-Dahlfors, Agneta / Melican, Keira

    Microbiology spectrum

    2019  Volume 7, Issue 3

    Abstract: Tissue microbiology allows for the study of bacterial infection in the most clinically relevant microenvironment, the living host. Advancements in techniques and technology have facilitated the development of novel ways of studying infection. Many of ... ...

    Abstract Tissue microbiology allows for the study of bacterial infection in the most clinically relevant microenvironment, the living host. Advancements in techniques and technology have facilitated the development of novel ways of studying infection. Many of these advancements have come from outside the field of microbiology. In this article, we outline the progression from bacteriology through cellular microbiology to tissue microbiology, highlighting seminal studies along the way. We outline the enormous potential but also some of the challenges of the tissue microbiology approach. We focus on the role of emerging technologies in the continual development of infectious disease research and highlight future possibilities in our ongoing quest to understand host-pathogen interaction.
    MeSH term(s) Animals ; Bacteria/pathogenicity ; Bacterial Infections/diagnostic imaging ; Bacterial Infections/microbiology ; Bacterial Infections/pathology ; Cellular Microenvironment ; Host-Pathogen Interactions ; Humans ; Microbiological Techniques/methods ; Microscopy/methods
    Language English
    Publishing date 2019-06-17
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2165-0497
    ISSN (online) 2165-0497
    DOI 10.1128/microbiolspec.BAI-0007-2019
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Dynamic single cell analysis in a proximal-tubule-on-chip reveals heterogeneous epithelial colonization strategies of uropathogenic

    Antypas, Haris / Zhang, Tianqi / Choong, Ferdinand X / Melican, Keira / Richter-Dahlfors, Agneta

    FEMS microbes

    2023  Volume 4, Page(s) xtad007

    Abstract: The urinary tract is a hydrodynamically challenging microenvironment and ... ...

    Abstract The urinary tract is a hydrodynamically challenging microenvironment and uropathogenic
    Language English
    Publishing date 2023-03-03
    Publishing country England
    Document type Journal Article
    ISSN 2633-6685
    ISSN (online) 2633-6685
    DOI 10.1093/femsmc/xtad007
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  6. Book ; Thesis: Regulation of the Cobalamin biosynthetic genes in Salmonella typhimurium

    Richter Dahlfors, Agneta A.

    (Comprehensive summaries of Uppsala dissertations from the Faculty of Science and Technology ; 78 ; Acta Universitatis Upsaliensis)

    1994  

    Author's details by Agneta A. Richter Dahlfors
    Series title Comprehensive summaries of Uppsala dissertations from the Faculty of Science and Technology ; 78
    Acta Universitatis Upsaliensis
    Collection
    Language English
    Size 62 S. : graph. Darst.
    Publisher Almqvist & Wiksell
    Publishing place Stockholm
    Publishing country Sweden
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Uppsala, Univ., Diss., 1994
    HBZ-ID HT007598081
    ISBN 91-554-3393-6 ; 978-91-554-3393-2
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

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    97 E 1656 order for loan Magazin

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  7. Article ; Online: Optotracing for live selective fluorescence-based detection of

    Kärkkäinen, Elina / Jakobsson, Saga G / Edlund, Ulrica / Richter-Dahlfors, Agneta / Choong, Ferdinand X

    Frontiers in cellular and infection microbiology

    2022  Volume 12, Page(s) 981454

    Abstract: ... Candida ... ...

    Abstract Candida albicans
    MeSH term(s) Antifungal Agents ; Biofilms ; Candida albicans ; Carbohydrates ; Chitin ; Humans ; Plant Extracts ; beta-Glucans
    Chemical Substances Antifungal Agents ; Carbohydrates ; Plant Extracts ; beta-Glucans ; Chitin (1398-61-4)
    Language English
    Publishing date 2022-09-02
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2022.981454
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  8. Article ; Online: Effect of anticoagulant and platelet inhibition on the risk of bacteremia among patients with acute pyelonephritis: a retrospective cohort study.

    Steiner, Svava E / Edgren, Gustaf / Melican, Keira / Richter-Dahlfors, Agneta / Brauner, Annelie

    BMC infectious diseases

    2022  Volume 22, Issue 1, Page(s) 509

    Abstract: Background: An increasing number of patients are being prescribed anticoagulants and platelet inhibitors (antithrombotic treatment). Basic research has suggested an association between antithrombotic treatment and bacteremia during kidney infection. ... ...

    Abstract Background: An increasing number of patients are being prescribed anticoagulants and platelet inhibitors (antithrombotic treatment). Basic research has suggested an association between antithrombotic treatment and bacteremia during kidney infection. Here, we investigated the association between antithrombotic treatment, bacteremia and acute kidney injury in patients with acute pyelonephritis.
    Methods: A retrospective cohort study was conducted in a large university hospital in Sweden. Data were retrieved from electronic medical records for adult patients with acute pyelonephritis in 2016. The main outcome was bacteremia and secondary outcome acute kidney injury. Odds ratios (ORs) with 95% confidence intervals (CIs) were estimated through multiple logistic regression. Treatment with different groups of antithrombotic agents were compared to no antithrombotic treatment.
    Results: 1814 patients with acute pyelonephritis were included, in whom bacteremia developed in 336 (18.5%). Low-molecular-weight heparin (LMWH) at prophylactic doses was associated with a lower risk of bacteremia, compared to no antithrombotic treatment (OR 0.5; 95% CI 0.3-0.7). Other antithrombotic treatments were not associated with a risk of bacteremia. Additionally, patients with prophylactic doses of LMWH had a lower risk of acute kidney injury (OR 0.5; 95% CI 0.3-0.8).
    Conclusions: We found no association between antithrombotic treatment and an increased risk of bacteremia during acute pyelonephritis. Conversely, patients with prophylactic doses of LMWH had a slightly reduced risk of bacteremia. LMWH at prophylactic doses was also associated with a lower risk of acute kidney injury. Our results suggest that it is safe to continue antithrombotic treatment during acute pyelonephritis, in regards to bacteremia and acute kidney injury risk.
    MeSH term(s) Acute Kidney Injury/complications ; Adult ; Anticoagulants/adverse effects ; Bacteremia/complications ; Bacteremia/drug therapy ; Fibrinolytic Agents ; Heparin, Low-Molecular-Weight ; Humans ; Pyelonephritis/drug therapy ; Retrospective Studies
    Chemical Substances Anticoagulants ; Fibrinolytic Agents ; Heparin, Low-Molecular-Weight
    Language English
    Publishing date 2022-05-31
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041550-3
    ISSN 1471-2334 ; 1471-2334
    ISSN (online) 1471-2334
    ISSN 1471-2334
    DOI 10.1186/s12879-022-07474-4
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  9. Article ; Online: An optotracer-based antibiotic susceptibility test specifically targeting the biofilm lifestyle of

    Eckert, Johannes A / Rosenberg, Ming / Rhen, Mikael / Choong, Ferdinand X / Richter-Dahlfors, Agneta

    Biofilm

    2022  Volume 4, Page(s) 100083

    Abstract: Antimicrobial resistance is a medical threat of global dimensions. Proper antimicrobial susceptibility testing (AST) for drug development, patient diagnosis and treatment is crucial to counteract ineffective drug use and resistance development. Despite ... ...

    Abstract Antimicrobial resistance is a medical threat of global dimensions. Proper antimicrobial susceptibility testing (AST) for drug development, patient diagnosis and treatment is crucial to counteract ineffective drug use and resistance development. Despite the important role of bacterial biofilms in chronic and device-associated infections, the efficacy of antibiotics is determined using planktonic cultures. To address the need for antibiotics targeting bacteria in the biofilm lifestyle, we here present an optotracing-based biofilm-AST using
    Language English
    Publishing date 2022-09-08
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2590-2075
    ISSN (online) 2590-2075
    DOI 10.1016/j.bioflm.2022.100083
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  10. Article ; Online: Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus.

    Butina, Karen / Lantz, Linda / Choong, Ferdinand X / Tomac, Ana / Shirani, Hamid / Löffler, Susanne / Nilsson, K Peter R / Richter-Dahlfors, Agneta

    Chembiochem : a European journal of chemical biology

    2022  Volume 23, Issue 11, Page(s) e202100684

    Abstract: Optotracers are conformation-sensitive fluorescent tracer molecules that detect peptide- and carbohydrate-based biopolymers. Their binding to bacterial cell walls allows selective detection and visualisation of Staphylococcus aureus (S. aureus). Here, we ...

    Abstract Optotracers are conformation-sensitive fluorescent tracer molecules that detect peptide- and carbohydrate-based biopolymers. Their binding to bacterial cell walls allows selective detection and visualisation of Staphylococcus aureus (S. aureus). Here, we investigated the structural properties providing optimal detection of S. aureus. We quantified spectral shifts and fluorescence intensity in mixes of bacteria and optotracers, using automatic peak analysis, cross-correlation, and area-under-curve analysis. We found that the length of the conjugated backbone and the number of charged groups, but not their distribution, are important factors for selective detection of S. aureus. The photophysical properties of optotracers were greatly improved by incorporating a donor-acceptor-donor (D-A-D)-type motif in the conjugated backbone. With significantly reduced background and binding-induced on-switch of fluorescence, these optotracers enabled real-time recordings of S. aureus growth. Collectively, this demonstrates that chemical structure and photophysics are key tunable characteristics in the development of optotracers for selective detection of bacterial species.
    MeSH term(s) Bacteria ; Fluorescence Resonance Energy Transfer ; Staphylococcus aureus
    Language English
    Publishing date 2022-04-01
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2020469-3
    ISSN 1439-7633 ; 1439-4227
    ISSN (online) 1439-7633
    ISSN 1439-4227
    DOI 10.1002/cbic.202100684
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