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  1. AU="Rieber, Julia"
  2. AU="Gulmuradov, Tashpulat"
  3. AU="Romeu Fontanillas, Teresa"
  4. AU="Fleming, Renée"
  5. AU="Cao, Fang"
  6. AU="Sally J L Moore"
  7. AU="Moreno, Yolanda"
  8. AU="Vasiliy Ya. Kolyuchkin"

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  1. Artikel ; Online: In Vitro and In Vivo Effects of IGF-1 Delivery Strategies on Tendon Healing: A Review.

    Miescher, Iris / Rieber, Julia / Calcagni, Maurizio / Buschmann, Johanna

    International journal of molecular sciences

    2023  Band 24, Heft 3

    Abstract: Tendon injuries suffer from a slow healing, often ending up in fibrovascular scar formation, leading to inferior mechanical properties and even re-rupture upon resumption of daily work or sports. Strategies including the application of growth factors ... ...

    Abstract Tendon injuries suffer from a slow healing, often ending up in fibrovascular scar formation, leading to inferior mechanical properties and even re-rupture upon resumption of daily work or sports. Strategies including the application of growth factors have been under view for decades. Insulin-like growth factor-1 (IGF-1) is one of the used growth factors and has been applied to tenocyte in vitro cultures as well as in animal preclinical models and to human patients due to its anabolic and matrix stimulating effects. In this narrative review, we cover the current literature on IGF-1, its mechanism of action, in vitro cell cultures (tenocytes and mesenchymal stem cells), as well as in vivo experiments. We conclude from this overview that IGF-1 is a potent stimulus for improving tendon healing due to its inherent support of cell proliferation, DNA and matrix synthesis, particularly collagen I, which is the main component of tendon tissue. Nevertheless, more in vivo studies have to be performed in order to pave the way for an IGF-1 application in orthopedic clinics.
    Mesh-Begriff(e) Animals ; Humans ; Insulin-Like Growth Factor I/pharmacology ; Tendons/metabolism ; Wound Healing ; Tendon Injuries/drug therapy ; Collagen Type I/metabolism
    Chemische Substanzen Insulin-Like Growth Factor I (67763-96-6) ; Collagen Type I
    Sprache Englisch
    Erscheinungsdatum 2023-01-25
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24032370
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Delineation of the healthy rabbit tongue by immunohistochemistry - A technical note.

    Meier Bürgisser, Gabriella / Heuberger, Dorothea M / Rieber, Julia / Miescher, Iris / Giovanoli, Pietro / Calcagni, Maurizio / Buschmann, Johanna

    Acta histochemica

    2023  Band 126, Heft 1, Seite(n) 152127

    Abstract: In the oral cavity the tongue is an important muscular organ that supports the swallowing of food and liquids. It is responsible for the sense of taste, based on the many different taste buds it contains. Research in the field of tongue diseases demands ... ...

    Abstract In the oral cavity the tongue is an important muscular organ that supports the swallowing of food and liquids. It is responsible for the sense of taste, based on the many different taste buds it contains. Research in the field of tongue diseases demands for suitable preclinical models. The healthy rabbit tongue may therefore serve as baseline and reference for the pathological situation. With this consideration, we covered the fixation and histological stainings as well as the immunohistochemical labelling of the healthy rabbit tongue. In this technical note, initial choice of the fixative is discussed, with a comparison of formalin fixation and subsequent paraffin embedding versus cryopreservation. Moreover, we delineate the effect of an antigen retrieval step for formalin fixation by several examples. Finally, we provide ECM markers collagen I, collagen III, fibronectin, α-SMA and elastin staining as well as ki67 for proliferative status and PAR-2 protein expression as a marker for inflammatory status and nociception in tongue sections, mainly from the tongue body. Technically, we found superiority of paraffin sections for collagen I, collagen III, fibronectin, ki67 and α-SMA labelling, for selected detections systems. As for ECM components, the lamina propria was very rich in collagen and fibronectin, while the muscular body of the tongue showed only collagen and fibronectin positive areas between the muscle fibers. Moreover, α-SMA was clearly expressed in the walls of arteries and veins. The inflammatory marker PAR-2 on the other hand was prominently expressed in the salivary glands and to some extent in the walls of the vessels. Particular PAR-2 expression was found in the excretory ducts of the tongue. This technical note has the aim to provide baseline images that can be used to compare the pathological state of the diseased rabbit tongue as well as for inter-species comparison, such as mouse or rat tongue. Finally, it can be used for the comparison with the human situation.
    Mesh-Begriff(e) Humans ; Rabbits ; Animals ; Rats ; Mice ; Immunohistochemistry ; Fibronectins/metabolism ; Ki-67 Antigen/metabolism ; Tongue/pathology ; Collagen/metabolism ; Formaldehyde
    Chemische Substanzen Fibronectins ; Ki-67 Antigen ; Collagen (9007-34-5) ; Formaldehyde (1HG84L3525)
    Sprache Englisch
    Erscheinungsdatum 2023-11-30
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 77-2
    ISSN 1618-0372 ; 0065-1281
    ISSN (online) 1618-0372
    ISSN 0065-1281
    DOI 10.1016/j.acthis.2023.152127
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: In Vitro Assessment of Bacterial Adhesion and Biofilm Formation on Novel Bioactive, Biodegradable Electrospun Fiber Meshes Intended to Support Tendon Rupture Repair.

    Miescher, Iris / Rieber, Julia / Schweizer, Tiziano A / Orlietti, Mariano / Tarnutzer, Andrea / Andreoni, Federica / Meier Buergisser, Gabriella / Giovanoli, Pietro / Calcagni, Maurizio / Snedeker, Jess G / Zinkernagel, Annelies S / Buschmann, Johanna

    ACS applied materials & interfaces

    2024  Band 16, Heft 5, Seite(n) 6348–6355

    Abstract: The surgical repair of a ruptured tendon faces two major problems: specifically increased fibrous adhesion to the surrounding tissue and inferior mechanical properties of the scar tissue compared to the native tissue. Bacterial attachment to implant ... ...

    Abstract The surgical repair of a ruptured tendon faces two major problems: specifically increased fibrous adhesion to the surrounding tissue and inferior mechanical properties of the scar tissue compared to the native tissue. Bacterial attachment to implant materials is an additional problem as it might lead to severe infections and impaired recovery. To counteract adhesion formation, two novel implant materials were fabricated by electrospinning, namely, a random fiber mesh containing hyaluronic acid (HA) and poly(ethylene oxide) (PEO) in a ratio of 1:1 (HA/PEO 1:1) and 1:4 (HA/PEO 1:4), respectively. Electrospun DegraPol (DP) treated with silver nanoparticles (DP-Ag) was developed to counteract the bacterial attachment. The three novel materials were compared to the previously described DP and DP with incorporated insulin-like growth factor-1 (DP-IGF-1), two implant materials that were also designed to improve tendon repair. To test whether the materials are prone to bacterial adhesion and biofilm formation, we assessed 10 strains of
    Mesh-Begriff(e) Humans ; Bacterial Adhesion ; Silver/pharmacology ; Silver/chemistry ; Insulin-Like Growth Factor I/pharmacology ; Metal Nanoparticles/chemistry ; Tendon Injuries/surgery ; Anti-Bacterial Agents/pharmacology ; Biofilms ; Tendons
    Chemische Substanzen Silver (3M4G523W1G) ; Insulin-Like Growth Factor I (67763-96-6) ; Anti-Bacterial Agents
    Sprache Englisch
    Erscheinungsdatum 2024-01-30
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 1944-8252
    ISSN (online) 1944-8252
    DOI 10.1021/acsami.3c15710
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Pro-angiogenic and antibacterial copper containing nanoparticles in PLGA/amorphous calcium phosphate bone nanocomposites.

    Näf, Lukas / Miescher, Iris / Pfuderer, Lara / Schweizer, Tiziano A / Brunner, David / Dürig, Johannes / Gröninger, Olivier / Rieber, Julia / Meier-Buergisser, Gabriella / Spanaus, Katharina / Calcagni, Maurizio / Bosshard, Philipp P / Achermann, Yvonne / Stark, Wendelin J / Buschmann, Johanna

    Heliyon

    2024  Band 10, Heft 5, Seite(n) e27267

    Abstract: Large bone defects after trauma demand for adequate bone substitutes. Bone void fillers should be antibacterial and pro-angiogenic. One viable option is the use of composite materials like the combination of PLGA and amorphous calcium phosphate (aCaP). ... ...

    Abstract Large bone defects after trauma demand for adequate bone substitutes. Bone void fillers should be antibacterial and pro-angiogenic. One viable option is the use of composite materials like the combination of PLGA and amorphous calcium phosphate (aCaP). Copper stimulates angiogenesis and has antibacterial qualities. Either copper oxide (CuO) nanoparticles (NPs) were therefore added to PLGA/aCaP/CuO in different concentrations (1, 5 and 10 w/w %) or copper-doped tricalcium phosphate NPs (TCP with 2% of copper) were electrospun into PLGA/CuTCP nanocomposites. Bi-layered nanocomposites of PLGA/aCaP with different copper NPs (CuO or TCP) and a second layer of pristine PLGA were fabricated. Two clinical bacterial isolates (
    Sprache Englisch
    Erscheinungsdatum 2024-03-04
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2024.e27267
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Bioactive and Elastic Emulsion Electrospun DegraPol Tubes Delivering IGF-1 for Tendon Rupture Repair.

    Rieber, Julia / Meier-Bürgisser, Gabriella / Miescher, Iris / Weber, Franz E / Wolint, Petra / Yao, Yang / Ongini, Esteban / Milionis, Athanasios / Snedeker, Jess G / Calcagni, Maurizio / Buschmann, Johanna

    International journal of molecular sciences

    2023  Band 24, Heft 12

    Abstract: Tendon injuries can result in two major drawbacks. Adhesions to the surrounding tissue may limit the range of motion, while fibrovascular scar formation can lead to poor biomechanical outcomes. Prosthetic devices may help to mitigate those problems. ... ...

    Abstract Tendon injuries can result in two major drawbacks. Adhesions to the surrounding tissue may limit the range of motion, while fibrovascular scar formation can lead to poor biomechanical outcomes. Prosthetic devices may help to mitigate those problems. Emulsion electrospinning was used to develop a novel three-layer tube based on the polymer DegraPol (DP), with incorporated insulin-like growth factor-1 (IGF-1) in the middle layer. Scanning electron microscopy was utilized to assess the fiber diameter in IGF-1 containing pure DP meshes. Further characterization was performed with Fourier Transformed Infrared Spectroscopy, Differential Scanning Calorimetry, and water contact angle, as well as through the assessment of mechanical properties and release kinetics from ELISA, and the bioactivity of IGF-1 by qPCR of
    Mesh-Begriff(e) Animals ; Rabbits ; Insulin-Like Growth Factor I/genetics ; Insulin-Like Growth Factor I/pharmacology ; Insulin-Like Growth Factor I/metabolism ; Emulsions/metabolism ; Ki-67 Antigen/metabolism ; Tendon Injuries/drug therapy ; Tendon Injuries/metabolism ; Achilles Tendon/metabolism
    Chemische Substanzen DegraPol ; Insulin-Like Growth Factor I (67763-96-6) ; Emulsions ; Ki-67 Antigen
    Sprache Englisch
    Erscheinungsdatum 2023-06-17
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241210272
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: Delineation of the healthy rabbit liver by immunohistochemistry - A technical note.

    Bürgisser, Gabriella Meier / Evrova, Olivera / Heuberger, Dorothea M / Rieber, Julia / Giovanoli, Pietro / Calcagni, Maurizio / Buschmann, Johanna

    Acta histochemica

    2021  Band 123, Heft 7, Seite(n) 151795

    Abstract: Liver diseases pose a big global health problem and liver failure may result from viral infection, overnutrition or tumors. Studying pathologic liver tissue demands for accurate and specific histological stainings and immunohistochemical labellings, ... ...

    Abstract Liver diseases pose a big global health problem and liver failure may result from viral infection, overnutrition or tumors. Studying pathologic liver tissue demands for accurate and specific histological stainings and immunohistochemical labellings, including chromogenic and fluorescent approaches. Moreover, a reliable set of healthy liver stainings and labellings are required, to provide a baseline or reference for the pathological situation. Here, we used the liver tissue of a healthy rabbit and compared different histological key steps, such as paraffin embedding after formalin fixation versus cryopreservation; or an antigen retrieval (AR) step in processing paraffin sections versus the same procedure without AR; or chromogenic with fluorescent detection system, respectively. Moreover, we provide images of serial sections, where we stained the same morphological structure with different markers, including collagen I, collagen III, fibronectin, α-SMA, elastin, protease-activated receptor-2 (PAR-2) which is an inflammation-related marker, ki67 for proliferating cells, and orcein (as negative control for pathological aberrations like Wilson disease). Differences between conditions were quantitatively assessed by measuring the colour intensity. Generally, we observed that cryosections exhibited a stronger signal intensity in immunohistochemically labelled sections than in paraffin sections; however, the strong staining got slurred, which sometimes hampered proper identification of morphological structures at higher magnifications. Moreover, there was a clear increase in signal intensity for paraffin sections when an AR step was performed compared to condition without AR. Results for mouse isotype staining as a negative control clearly supported those findings. Different stainings of the portal triad, the central vein and the bile ducts revealed a clear-cut distribution of extracellular matrix components, with prominent fibronectin and elastin around the lumen of the central vein as well as a patchy PAR-2 expression. As for the bile ducts, complete absence of α-SMA and PAR-2 was found at the margins, however, collagen I expression and elastin were positive and showed a strong signal. Like this, we provide useful and valuable reference images for researchers using the rabbit liver model. It may help to decide which of the immunohistochemical protocols are valuable to reach a certain aim and which protocols lead to the best visualization of the target structure.
    Mesh-Begriff(e) Animals ; Immunohistochemistry ; Liver/cytology ; Liver/metabolism ; Rabbits
    Sprache Englisch
    Erscheinungsdatum 2021-10-07
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 77-2
    ISSN 1618-0372 ; 0065-1281
    ISSN (online) 1618-0372
    ISSN 0065-1281
    DOI 10.1016/j.acthis.2021.151795
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Electrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression.

    Bürgisser, Gabriella Meier / Evrova, Olivera / Heuberger, Dorothea M / Wolint, Petra / Rieber, Julia / Miescher, Iris / Schüpbach, Reto A / Giovanoli, Pietro / Calcagni, Maurizio / Buschmann, Johanna

    Scientific reports

    2021  Band 11, Heft 1, Seite(n) 23293

    Abstract: One great challenge in surgical tendon repair is the minimization of peritendinous adhesions. An electrospun tube can serve as a physical barrier around a conventionally sutured tendon. Six New Zealand White rabbits had one Achilles tendon fully ... ...

    Abstract One great challenge in surgical tendon repair is the minimization of peritendinous adhesions. An electrospun tube can serve as a physical barrier around a conventionally sutured tendon. Six New Zealand White rabbits had one Achilles tendon fully transsected and sutured by a 4-strand suture. Another six rabbits had the same treatment, but with the additional electrospun DegraPol tube set around the sutured tendon. The adhesion formation to the surrounding tissue was investigated 12 weeks post-operation. Moreover, inflammation-related protease-activated receptor-2 (PAR-2) protein expression was assessed. Finally, rabbit Achilles tenocyte cultures were exposed to platelet-derived growth factor-BB (PDGF-BB), which mimicks the tendon healing environment, where PAR-2 gene expression was assessed as well as immunofluorescent staining intensity for F-actin and α-tubulin, respectively. At 12 weeks post-operation, the partially degraded DegraPol tube exhibited significantly lower adhesion formation (- 20%). PAR-2 protein expression was similar for time points 3 and 6 weeks, but increased at 12 weeks post-operation. In vitro cell culture experiments showed a significantly higher PAR-2 gene expression on day 3 after exposure to PDGF-BB, but not on day 7. The cytoskeleton of the tenocytes changed upon PDGF-BB stimulation, with signs of reorganization, and significantly decreased F-actin intensity. An electrospun DegraPol tube significantly reduces adhesion up to twelve weeks post-operation. At this time point, the tube is partially degraded, and a slight PAR-2 increase was detected in the DP treated tendons, which might however arise from particles of degrading DegraPol that were stained dark brown. PAR-2 gene expression in rabbit tenocytes reveals sensitivity at around day 10 after injury.
    Mesh-Begriff(e) Achilles Tendon/surgery ; Animals ; Cells, Cultured ; Gene Expression ; Musculoskeletal Diseases/genetics ; Musculoskeletal Diseases/prevention & control ; Orthopedic Procedures/methods ; Polyesters ; Polyurethanes ; Postoperative Complications/genetics ; Postoperative Complications/prevention & control ; Rabbits ; Receptor, PAR-2/genetics ; Receptor, PAR-2/metabolism ; Suture Techniques ; Tendon Injuries/surgery ; Tenocytes/metabolism ; Time Factors ; Tissue Adhesions/genetics ; Tissue Adhesions/prevention & control
    Chemische Substanzen DegraPol ; Polyesters ; Polyurethanes ; Receptor, PAR-2
    Sprache Englisch
    Erscheinungsdatum 2021-12-02
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-02780-4
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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