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  1. Article ; Online: PEG Reinforced Scaffold Promotes Uniform Distribution of Human MSC-Created Cartilage Matrix.

    Riewruja, Kanyakorn / Aguglia, Alyssa M / Hines, Sophie / Makarcyzk, Meagan J / Honsawek, Sittisak / Lin, Hang

    Gels (Basel, Switzerland)

    2022  Volume 8, Issue 12

    Abstract: Previously, we used a gelatin/hyaluronic acid (GH)-based scaffold to induce chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells (hBMSC). The results showed that hBMSCs underwent robust chondrogenesis and ... ...

    Abstract Previously, we used a gelatin/hyaluronic acid (GH)-based scaffold to induce chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells (hBMSC). The results showed that hBMSCs underwent robust chondrogenesis and facilitated
    Language English
    Publishing date 2022-12-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2813982-3
    ISSN 2310-2861 ; 2310-2861
    ISSN (online) 2310-2861
    ISSN 2310-2861
    DOI 10.3390/gels8120794
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Experimental models to study osteoarthritis pain and develop therapeutics.

    Riewruja, Kanyakorn / Makarczyk, Meagan / Alexander, Peter G / Gao, Qi / Goodman, Stuart B / Bunnell, Bruce A / Gold, Michael S / Lin, Hang

    Osteoarthritis and cartilage open

    2022  Volume 4, Issue 4, Page(s) 100306

    Abstract: Pain is the predominant symptom of osteoarthritis (OA) that drives patients to seek medical care. Currently, there are no pharmacological treatments that can reverse or halt the progression of OA. Safe and efficacious medications for long-term management ...

    Abstract Pain is the predominant symptom of osteoarthritis (OA) that drives patients to seek medical care. Currently, there are no pharmacological treatments that can reverse or halt the progression of OA. Safe and efficacious medications for long-term management of OA pain are also unavailable. Understanding the mechanisms behind OA pain generation at onset and over time is critical for developing effective treatments. In this narrative review, we first summarize our current knowledge on the innervation of the knee joint, and then discuss the molecular mechanism(s) currently thought to underlie OA pain. In particular, we focus on the contribution of each joint component to the generation of pain. Next, the current experimental models for studying OA pain are summarized, and the methods to assess pain in rodents are presented. The potential application of emerging microphysiological systems in OA pain research is especially highlighted. Lastly, we discuss the current challenge in standardizing models and the selection of appropriate systems to address specific questions.
    Language English
    Publishing date 2022-08-13
    Publishing country England
    Document type Journal Article ; Review
    ISSN 2665-9131
    ISSN (online) 2665-9131
    DOI 10.1016/j.ocarto.2022.100306
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Cytokine Profiling and Intra-Articular Injection of Autologous Platelet-Rich Plasma in Knee Osteoarthritis.

    Riewruja, Kanyakorn / Phakham, Suphattra / Sompolpong, Patlapa / Reantragoon, Rangsima / Tanavalee, Aree / Ngarmukos, Srihatach / Udomsinprasert, Wanvisa / Suantawee, Tanyawan / Dechsupa, Sinsuda / Honsawek, Sittisak

    International journal of molecular sciences

    2022  Volume 23, Issue 2

    Abstract: Osteoarthritis (OA) is a degenerative joint disease leading to joint pain and stiffness. Due to lack of effective treatments, physical and psychological disabilities caused by OA have a detrimental impact on the patient's quality of life. Emerging ... ...

    Abstract Osteoarthritis (OA) is a degenerative joint disease leading to joint pain and stiffness. Due to lack of effective treatments, physical and psychological disabilities caused by OA have a detrimental impact on the patient's quality of life. Emerging evidence suggests that intra-articular injection of platelet-rich plasma (PRP) may provide favorable results since PRP comprises not only a high level of platelets but also a huge amount of cytokines, chemokines, and growth factors. However, the precise mechanism and standardization method remain uncertain. This study aimed to examine cytokine profiling in both PRP and platelet-poor plasma (PPP) of knee OA patients and to determine the effects of PRP on OA chondrocytes and knee OA patients. PRP contained a wide variety of cytokines, chemokines, growth factors, and autologous intra-articular PRP injection resulted in favorable outcomes in knee OA patients. Significant increases in levels of IL-1, IL-2, IL-7, IL-8, IL-9, IL-12, TNF-α, IL-17, PDGF-BB, bFGF, and MIP-1β were detected in PRP compared to PPP (
    MeSH term(s) Aged ; Biomarkers ; Cell Movement ; Cell Proliferation ; Chondrocytes/metabolism ; Cytokines/metabolism ; Disease Management ; Disease Susceptibility ; Female ; Humans ; Inflammation Mediators ; Male ; Middle Aged ; Osteoarthritis, Knee/diagnosis ; Osteoarthritis, Knee/etiology ; Osteoarthritis, Knee/metabolism ; Osteoarthritis, Knee/therapy ; Platelet-Rich Plasma ; Treatment Outcome
    Chemical Substances Biomarkers ; Cytokines ; Inflammation Mediators
    Language English
    Publishing date 2022-01-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23020890
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Neutrophil-to-lymphocyte ratio predicts the severity of motor impairment in cerebral palsy children living at home and the rehabilitation center: A comparative study.

    Riewruja, Kanyakorn / Amarase, Chavarin / Osateerakun, Phatcharapa / Weerasopone, Soarawit / Limpaphayom, Noppachart / Honsawek, Sittisak

    Biomedical reports

    2020  Volume 13, Issue 6, Page(s) 63

    Abstract: The aim of the present study was to investigate the neutrophil-to-lymphocyte ratio (NLR) level in children with cerebral palsy (CP) living at home or the rehabilitation center. The correlation of NLR with different severities of motor impairment was ... ...

    Abstract The aim of the present study was to investigate the neutrophil-to-lymphocyte ratio (NLR) level in children with cerebral palsy (CP) living at home or the rehabilitation center. The correlation of NLR with different severities of motor impairment was assessed. This was a single-center, cross-sectional, observational study. A total of 80 CP children who were either living at home (n=34) or at the rehabilitation center (n=46) were included. Demographic characteristics, anthropometric parameters and complete blood counts were recorded, and the NLR values were calculated. The severity of motor impairment was evaluated and categorized according to the Gross Motor Functional Classification System (GMFCS) level. The mean age of CP participants was 8.52±1.92 years. The percentage of children with CP who were malnourished (underweight, stunted and wasted) was higher amongst those at the rehabilitation center compared with those living at home. The mean NLR of children with CP in the rehabilitation center was significantly higher compared with the patients living at home (P=0.003). Participants from the rehabilitation center had severe motor impairment (GMFCS levels IV-V) and significantly higher NLR values than those with mild motor impairments (GMFCS levels II-III; P=0.006). However, there were no differences in NLR values in relation to severity of motor impairment in CP children living at home. CP children had some degree of neuroinflammation and systemic inflammation. NLR may be a potential simple inflammatory parameter that may be used to predict the severity of the motor impairment, particularly in CP children living at a rehabilitation center.
    Language English
    Publishing date 2020-10-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2763624-0
    ISSN 2049-9442 ; 2049-9434
    ISSN (online) 2049-9442
    ISSN 2049-9434
    DOI 10.3892/br.2020.1370
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Differences in the intrinsic chondrogenic potential of human mesenchymal stromal cells and iPSC-derived multipotent cells.

    Xiang, Shiqi / Lin, Zixuan / Makarcyzk, Meagan J / Riewruja, Kanyakorn / Zhang, Yiqian / Zhang, Xiurui / Li, Zhong / Clark, Karen L / Li, Eileen / Liu, Silvia / Hao, Tingjun / Fritch, Madalyn R / Alexander, Peter G / Lin, Hang

    Clinical and translational medicine

    2021  Volume 12, Issue 12, Page(s) e1112

    Abstract: Background: Human multipotent progenitor cells (hiMPCs) created from induced pluripotent stem cells (iPSCs) represent a new cell source for cartilage regeneration. In most studies, bone morphogenetic proteins (BMPs) are needed to enhance transforming ... ...

    Abstract Background: Human multipotent progenitor cells (hiMPCs) created from induced pluripotent stem cells (iPSCs) represent a new cell source for cartilage regeneration. In most studies, bone morphogenetic proteins (BMPs) are needed to enhance transforming growth factor-β (TGFβ)-induced hiMPC chondrogenesis. In contrast, TGFβ alone is sufficient to result in robust chondrogenesis of human primary mesenchymal stromal cells (hMSCs). Currently, the mechanism underlying this difference between hiMPCs and hMSCs has not been fully understood.
    Methods: In this study, we first tested different growth factors alone or in combination in stimulating hiMPC chondrogenesis, with a special focus on chondrocytic hypertrophy. The reparative capacity of hiMPCs-derived cartilage was assessed in an osteochondral defect model created in rats. hMSCs isolated from bone marrow were included in all studies as the control. Lastly, a mechanistic study was conducted to understand why hiMPCs and hMSCs behave differently in responding to TGFβ.
    Results: Chondrogenic medium supplemented with TGFβ3 and BMP6 led to robust in vitro cartilage formation from hiMPCs with minimal hypertrophy. Cartilage tissue generated from this new method was resistant to osteogenic transition upon subcutaneous implantation and resulted in a hyaline cartilage-like regeneration in osteochondral defects in rats. Interestingly, TGFβ3 induced phosphorylation of both Smad2/3 and Smad1/5 in hMSCs, but only activated Smad2/3 in hiMPCs. Supplementing BMP6 activated Smad1/5 and significantly enhanced TGFβ's compacity in inducing hiMPC chondrogenesis. The chondro-promoting function of BMP6 was abolished by the treatment of a BMP pathway inhibitor.
    Conclusions: This study describes a robust method to generate chondrocytes from hiMPCs with low hypertrophy for hyaline cartilage repair, as well as elucidates the difference between hMSCs and hiMPCs in response to TGFβ. Our results also indicated the importance of activating both Smad2/3 and Smad1/5 in the initiation of chondrogenesis.
    MeSH term(s) Humans ; Rats ; Animals ; Induced Pluripotent Stem Cells ; Chondrogenesis/physiology ; Mesenchymal Stem Cells/metabolism ; Transforming Growth Factor beta/metabolism ; Hypertrophy/metabolism
    Chemical Substances Transforming Growth Factor beta
    Language English
    Publishing date 2021-07-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
    ZDB-ID 2697013-2
    ISSN 2001-1326 ; 2001-1326
    ISSN (online) 2001-1326
    ISSN 2001-1326
    DOI 10.1002/ctm2.1112
    Database MEDical Literature Analysis and Retrieval System OnLINE

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