Article ; Online: A novel assay for the detection of anti-human platelet antigen antibodies (HPA-1a) based on peptide aptamer technology.
2011 Volume 97, Issue 5, Page(s) 696–704
Abstract: Background: Neonatal alloimmune thrombocytopenia is mostly due to the presence of maternal antibodies against the fetal platelet antigen HPA-1a on the platelet integrin GPIIb-IIIa. Accurate detection of anti-HPA-1a antibodies in the mother is, therefore, ...
| Abstract | Background: Neonatal alloimmune thrombocytopenia is mostly due to the presence of maternal antibodies against the fetal platelet antigen HPA-1a on the platelet integrin GPIIb-IIIa. Accurate detection of anti-HPA-1a antibodies in the mother is, therefore, critical. Current diagnostic assays rely on the availability of pools of human platelets that vary according to donors and blood centers. There is still no satisfactory standardization of these assays. Design and methods: Peptide aptamer was used to detect and identify HPA-1a-specific antibodies in human serum that do not require human platelets. A peptide aptamer library was screened using an anti-HPA-1a human monoclonal antibody as a bait to isolate an aptamer that mimics the human platelet antigen HPA-1a. Results: This is the first report in platelet immunology of the use of a peptide aptamer for diagnostic purposes. This assay gives better results than the MAIPA currently in use, detecting around 90% of the expected alloantibodies. Conclusions: This assay could help define a standard for the quantitation of anti-HPA antibodies. This report also demonstrates that peptide aptamers can potentially detect a variety of biomarkers in body fluids; this is of particular interest for diagnostic purposes. |
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| MeSH term(s) | Amino Acid Sequence ; Antibodies, Anti-Idiotypic/blood ; Antibodies, Anti-Idiotypic/immunology ; Antibodies, Monoclonal/immunology ; Antigen-Antibody Complex ; Antigens, Human Platelet/immunology ; Aptamers, Nucleotide ; Blood Platelets/immunology ; Blotting, Western ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoassay ; Immunoprecipitation ; Infant, Newborn ; Isoantibodies/blood ; Isoantibodies/immunology ; Molecular Sequence Data ; Peptide Fragments ; Platelet Glycoprotein GPIIb-IIIa Complex/immunology ; Thrombocytopenia, Neonatal Alloimmune/blood ; Thrombocytopenia, Neonatal Alloimmune/diagnosis ; Thrombocytopenia, Neonatal Alloimmune/immunology |
| Chemical Substances | Antibodies, Anti-Idiotypic ; Antibodies, Monoclonal ; Antigen-Antibody Complex ; Antigens, Human Platelet ; Aptamers, Nucleotide ; ITGB3 protein, human ; Isoantibodies ; Peptide Fragments ; Platelet Glycoprotein GPIIb-IIIa Complex |
| Language | English |
| Publishing date | 2011-12-01 |
| Publishing country | Italy |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2333-4 |
| ISSN | 1592-8721 ; 0017-6567 ; 0390-6078 |
| ISSN (online) | 1592-8721 |
| ISSN | 0017-6567 ; 0390-6078 |
| DOI | 10.3324/haematol.2011.051276 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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