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  1. Article: Current application of chemometrics in traditional Chinese herbal medicine research

    Huang, Yipeng / Fuyou Du / Gongke Li / Guihua Ruan / Rihui Su / Zhenwei Wu

    Journal of chromatography. 2016 July 15, v. 1026

    2016  

    Abstract: Traditional Chinese herbal medicines (TCHMs) are promising approach for the treatment of various diseases which have attracted increasing attention all over the world. Chemometrics in quality control of TCHMs are great useful tools that harnessing ... ...

    Abstract Traditional Chinese herbal medicines (TCHMs) are promising approach for the treatment of various diseases which have attracted increasing attention all over the world. Chemometrics in quality control of TCHMs are great useful tools that harnessing mathematics, statistics and other methods to acquire information maximally from the data obtained from various analytical approaches. This feature article focuses on the recent studies which evaluating the pharmacological efficacy and quality of TCHMs by determining, identifying and discriminating the bioactive or marker components in different samples with the help of chemometric techniques. In this work, the application of chemometric techniques in the classification of TCHMs based on their efficacy and usage was introduced. The recent advances of chemometrics applied in the chemical analysis of TCHMs were reviewed in detail.
    Keywords chemical analysis ; chemometrics ; chromatography ; herbal medicines ; mathematics ; quality control ; statistics
    Language English
    Dates of publication 2016-0715
    Size p. 27-35.
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 1570-0232
    DOI 10.1016/j.jchromb.2015.12.050
    Database NAL-Catalogue (AGRICOLA)

    Full text online

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  2. Article: Novel regenerative large-volume immobilized enzyme reactor: Preparation, characterization and application

    Ruan, Guihua / Fuyou Du / Meiping Wei / Rihui Su / Yanjie Zheng / Zhengyi Chen

    Journal of Chromatography B. 2014 Sept. 15, v. 967

    2014  

    Abstract: A novel large-volume immobilized enzyme reactor (IMER) on small column was prepared with organic–inorganic hybrid silica particles and applied for fast (10min) and oriented digestion of protein. At first, a thin enzyme support layer was formed in the ... ...

    Abstract A novel large-volume immobilized enzyme reactor (IMER) on small column was prepared with organic–inorganic hybrid silica particles and applied for fast (10min) and oriented digestion of protein. At first, a thin enzyme support layer was formed in the bottom of the small column by polymerization with α-methacrylic acid and dimethacrylate. After that, amino SiO2 particles was prepared by the sol–gel method with tetraethoxysilane and 3-aminopropyltriethoxysilane. Subsequently, the amino SiO2 particles were activated by glutaraldehyde for covalent immobilization of trypsin. Digestive capability of large-volume IMER for proteins was investigated by using bovine serum albumin (BSA), cytochrome c (Cyt-c) as model proteins. Results showed that although the sequence coverage of the BSA (20%) and Cyt-c (19%) was low, the large-volume IMER could produce peptides with stable specific sequence at 101–105, 156–160, 205–209, 212–218, 229–232, 257–263 and 473–451 of the amino sequence of BSA when digesting 1mg/mL BSA. Eight of common peptides were observed during each of the ten runs of large-volume IMER. Besides, the IMER could be easily regenerated by reactivating with GA and cross-linking with trypsin after breaking the –CN– bond by 0.01M HCl. The sequence coverage of BSA from regenerated IMER increased to 25% comparing the non-regenerated IMER (17%). 14 common peptides. accounting for 87.5% of first use of IMER, were produced both with IMER and regenerated IMER. When the IMER was applied for ginkgo albumin digestion, the sequence coverage of two main proteins of ginkgo, ginnacin and legumin, was 56% and 55%, respectively. (Reviewer 2) Above all, the fast and selective digestion property of the large-volume IMER indicated that the regenerative IMER could be tentatively used for the production of potential bioactive peptides and the study of oriented protein digestion.
    Keywords bovine serum albumin ; chromatography ; crosslinking ; cytochrome c ; Ginkgo ; glutaraldehyde ; hydrochloric acid ; immobilized enzymes ; legumin ; peptides ; polymerization ; silica ; sol-gel processing ; trypsin
    Language English
    Dates of publication 2014-0915
    Size p. 13-20.
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 1570-0232
    DOI 10.1016/j.jchromb.2014.07.008
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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