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Artikel ; Online: Engineering microbial fuels cells: recent patents and new directions.

Biffinger, Justin C / Ringeisen, Bradley R

Recent patents on biotechnology

2008 Band 2, Heft 3, Seite(n) 150–155

Abstract: Fundamental research into how microbes generate electricity within microbial fuel cells (MFCs) has far outweighed the practical application and large scale development of microbial energy harvesting devices. MFCs are considered alternatives to standard ... ...

Abstract	Fundamental research into how microbes generate electricity within microbial fuel cells (MFCs) has far outweighed the practical application and large scale development of microbial energy harvesting devices. MFCs are considered alternatives to standard commercial polymer electrolyte membrane (PEM) fuel cell technology because the fuel supply does not need to be purified, ambient operating temperatures are maintained with biologically compatible materials, and the biological catalyst is self-regenerating. The generation of electricity during wastewater treatment using MFCs may profoundly affect the approach to anaerobic treatment technologies used in wastewater treatment as a result of developing this energy harvesting technology. However, the materials and engineering designs for MFCs were identical to commercial fuel cells until 2003. Compared to commercial fuel cells, MFCs will remain underdeveloped as long as low power densities are generated from the best systems. The variety of designs for MFCs has expanded rapidly in the last five years in the literature, but the patent protection has lagged behind. This review will cover recent and important patents relating to MFC designs and progress.
Mesh-Begriff(e)	Bioelectric Energy Sources/microbiology ; Bioelectric Energy Sources/trends ; Biomedical Engineering/instrumentation ; Biomedical Engineering/trends ; Bioreactors/microbiology ; Biotechnology/trends ; Equipment Design ; Forecasting ; Patents as Topic ; Technology Assessment, Biomedical
Sprache	Englisch
Erscheinungsdatum	2008-08-19
Erscheinungsland	United Arab Emirates
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ISSN	2212-4012
ISSN (online)	2212-4012
DOI	10.2174/187220808786240962
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Buch: Cell and organ printing

Ringeisen, Bradley R / Spargo, Barry J / Wu, Peter K

2010

Verfasserangabe	Bradley R. Ringeisen, Barry J. Spargo, Peter K. Wu, editors
Mesh-Begriff(e)	Biotechnology/methods ; Cell Culture Techniques/methods ; Organ Culture Techniques/methods ; Tissue Engineering/methods ; Printing/methods
Sprache	Englisch
Umfang	xiv, 260 p. :, ill. ;, 24 cm.
Verlag	Springer
Erscheinungsort	Dordrecht ; New York
Dokumenttyp	Buch
ISBN	9789048191444 ; 9048191440 ; 9789048191451 ; 9048191459
Datenquelle	Katalog der US National Library of Medicine (NLM)

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Artikel: Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material.

Gaston, Joel D / Bischel, Lauren L / Fitzgerald, Lisa A / Cusick, Kathleen D / Ringeisen, Bradley R / Pirlo, Russell K

Journal of healthcare engineering

2017 Band 2017, Seite(n) 5740975

Abstract: Disruption of the blood-brain barrier (BBB) is the hallmark of many neurovascular disorders, making it a critically important focus for therapeutic options. However, testing the effects of either drugs or pathological agents is difficult due to the ... ...

Abstract	Disruption of the blood-brain barrier (BBB) is the hallmark of many neurovascular disorders, making it a critically important focus for therapeutic options. However, testing the effects of either drugs or pathological agents is difficult due to the potentially damaging consequences of altering the normal brain microenvironment. Recently, in vitro coculture tissue models have been developed as an alternative to animal testing. Despite low cost, these platforms use synthetic scaffolds which prevent normal barrier architecture, cellular crosstalk, and tissue remodeling. We created a biodegradable electrospun gelatin mat "biopaper" (BP) as a scaffold material for an endothelial/astrocyte coculture model allowing cell-cell contact and crosstalk. To compare the BP and traditional models, we investigated the expression of 27 genes involved in BBB permeability, cellular function, and endothelial junctions at different time points. Gene expression levels demonstrated higher expression of transcripts involved in endothelial junction formation, including TJP2 and CDH5, in the BP model. The traditional model had higher expression of genes associated with extracellular matrix-associated proteins, including SPARC and COL4A1. Overall, the results demonstrate that the BP coculture model is more representative of a healthy BBB state, though both models have advantages that may be useful in disease modeling.
Mesh-Begriff(e)	Biocompatible Materials ; Biological Transport ; Blood-Brain Barrier ; Coculture Techniques ; Gene Expression ; Humans ; Models, Biological ; Permeability
Chemische Substanzen	Biocompatible Materials
Sprache	Englisch
Erscheinungsdatum	2017-11-29
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ISSN	2040-2295
ISSN	2040-2295
DOI	10.1155/2017/5740975
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: High frequency of glucose-utilizing mutants in Shewanella oneidensis MR-1.

Howard, Erinn C / Hamdan, Leila J / Lizewski, Stephen E / Ringeisen, Bradley R

FEMS microbiology letters

2012 Band 327, Heft 1, Seite(n) 9–14

Abstract: Shewanella oneidensis MR-1 has conventionally been considered unable to use glucose as a carbon substrate for growth. The genome sequence of S. oneidensis MR-1 however suggests the ability to use glucose. Here, we demonstrate that during initial glucose ... ...

Abstract	Shewanella oneidensis MR-1 has conventionally been considered unable to use glucose as a carbon substrate for growth. The genome sequence of S. oneidensis MR-1 however suggests the ability to use glucose. Here, we demonstrate that during initial glucose exposure, S. oneidensis MR-1 quickly and frequently gains the ability to utilize glucose as a sole carbon source, in contrast to wild-type S. oneidensis, which cannot immediately use glucose as a sole carbon substrate. High-performance liquid chromatography and (14)C glucose tracer studies confirm the disappearance in cultures and assimilation and respiration, respectively, of glucose. The relatively short time frame with which S. oneidensis MR-1 gained the ability to use glucose raises interesting ecological implications.
Mesh-Begriff(e)	Glucose/metabolism ; Mutation ; Shewanella/genetics ; Shewanella/growth & development ; Shewanella/metabolism
Chemische Substanzen	Glucose (IY9XDZ35W2)
Sprache	Englisch
Erscheinungsdatum	2012-02
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	752343-9
ISSN	1574-6968 ; 0378-1097
ISSN (online)	1574-6968
ISSN	0378-1097
DOI	10.1111/j.1574-6968.2011.02450.x
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Robotic RNA extraction for SARS-CoV-2 surveillance using saliva samples.

PloS one

2021 Band 16, Heft 8, Seite(n) e0255690

Abstract: Saliva is an attractive specimen type for asymptomatic surveillance of COVID-19 in large populations due to its ease of collection and its demonstrated utility for detecting RNA from SARS-CoV-2. Multiple saliva-based viral detection protocols use a ... ...

Abstract	Saliva is an attractive specimen type for asymptomatic surveillance of COVID-19 in large populations due to its ease of collection and its demonstrated utility for detecting RNA from SARS-CoV-2. Multiple saliva-based viral detection protocols use a direct-to-RT-qPCR approach that eliminates nucleic acid extraction but can reduce viral RNA detection sensitivity. To improve test sensitivity while maintaining speed, we developed a robotic nucleic acid extraction method for detecting SARS-CoV-2 RNA in saliva samples with high throughput. Using this assay, the Free Asymptomatic Saliva Testing (IGI FAST) research study on the UC Berkeley campus conducted 11,971 tests on supervised self-collected saliva samples and identified rare positive specimens containing SARS-CoV-2 RNA during a time of low infection prevalence. In an attempt to increase testing capacity, we further adapted our robotic extraction assay to process pooled saliva samples. We also benchmarked our assay against nasopharyngeal swab specimens and found saliva methods require further optimization to match this gold standard. Finally, we designed and validated a RT-qPCR test suitable for saliva self-collection. These results establish a robotic extraction-based procedure for rapid PCR-based saliva testing that is suitable for samples from both symptomatic and asymptomatic individuals.
Mesh-Begriff(e)	Adult ; COVID-19/diagnosis ; COVID-19 Testing/methods ; Female ; Humans ; Male ; Mass Screening/methods ; RNA/genetics ; RNA/isolation & purification ; RNA, Viral/genetics ; RNA, Viral/isolation & purification ; Real-Time Polymerase Chain Reaction/methods ; Robotics/methods ; SARS-CoV-2/genetics ; Saliva/chemistry ; Specimen Handling/methods
Chemische Substanzen	RNA, Viral ; RNA (63231-63-0)
Sprache	Englisch
Erscheinungsdatum	2021-08-05
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0255690
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Robotic RNA extraction for SARS-CoV-2 surveillance using saliva samples.

medRxiv : the preprint server for health sciences

2021

Abstract	Saliva is an attractive specimen type for asymptomatic surveillance of COVID-19 in large populations due to its ease of collection and its demonstrated utility for detecting RNA from SARS-CoV-2. Multiple saliva-based viral detection protocols use a direct-to-RT-qPCR approach that eliminates nucleic acid extraction but can reduce viral RNA detection sensitivity. To improve test sensitivity while maintaining speed, we developed a robotic nucleic acid extraction method for detecting SARS-CoV-2 RNA in saliva samples with high throughput. Using this assay, the Free Asymptomatic Saliva Testing (IGI-FAST) research study on the UC Berkeley campus conducted 11,971 tests on supervised self-collected saliva samples and identified rare positive specimens containing SARS-CoV-2 RNA during a time of low infection prevalence. In an attempt to increase testing capacity, we further adapted our robotic extraction assay to process pooled saliva samples. We also benchmarked our assay against the gold standard, nasopharyngeal swab specimens. Finally, we designed and validated a RT-qPCR test suitable for saliva self-collection. These results establish a robotic extraction-based procedure for rapid PCR-based saliva testing that is suitable for samples from both symptomatic and asymptomatic individuals.
Sprache	Englisch
Erscheinungsdatum	2021-01-29
Erscheinungsland	United States
Dokumenttyp	Preprint
DOI	10.1101/2021.01.10.21249151
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: IGI-LuNER: single-well multiplexed RT-qPCR test for SARS-CoV-2.

medRxiv : the preprint server for health sciences

2020

Abstract: Commonly used RT-qPCR-based SARS-CoV-2 diagnostics require 2-3 separate reactions or rely on detection of a single viral target, adding time and cost or risk of false-negative results. Currently, no test combines detection of widely used SARS-CoV-2 E- ... ...

Abstract	Commonly used RT-qPCR-based SARS-CoV-2 diagnostics require 2-3 separate reactions or rely on detection of a single viral target, adding time and cost or risk of false-negative results. Currently, no test combines detection of widely used SARS-CoV-2 E- and N-gene targets and a sample control in a single, multiplexed reaction. We developed the IGI-LuNER RT-qPCR assay using the Luna Probe Universal One-Step RT-qPCR master mix with publicly available primers and probes to detect SARS-CoV-2 N gene, E gene, and human RNase P (NER). This combined, cost-effective test can be performed in 384-well plates with detection sensitivity suitable for clinical reporting, and will aid in future sample pooling efforts, thus improving throughput of SARS-CoV-2 detection.
Sprache	Englisch
Erscheinungsdatum	2020-12-11
Erscheinungsland	United States
Dokumenttyp	Preprint
DOI	10.1101/2020.12.10.20247338
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Optimal method for efficiently removing extracellular nanofilaments from Shewanella oneidensis MR-1.

Howard, Erinn C / Petersen, Emily R / Fitzgerald, Lisa A / Sheehan, Paul E / Ringeisen, Bradley R

Journal of microbiological methods

2011 Band 87, Heft 3, Seite(n) 320–324

Abstract: The identification, production, and potential electron conductivity of bacterial extracellular nanofilaments is an area of great study, specifically in Shewanella oneidensis MR-1. While some studies focus on nanofilaments attached to the cellular body, ... ...

Abstract	The identification, production, and potential electron conductivity of bacterial extracellular nanofilaments is an area of great study, specifically in Shewanella oneidensis MR-1. While some studies focus on nanofilaments attached to the cellular body, many studies require the removal of these nanofilaments for downstream applications. The removal of nanofilaments from S. oneidensis MR-1 for further study requires not only that the nanofilaments be detached, but also for the cell bodies to remain intact. This is a study to both qualitatively (AFM) and quantitatively (LC/MS-MS) assess several nanofilament shearing methods and determine the optimal procedure. The best method for nanofilament removal, as judged by maximizing extracellular filamentous proteins and minimizing membrane and intracellular proteins, is vortexing a washed cell culture for 10 min.
Mesh-Begriff(e)	Chromatography, Liquid ; Microscopy, Atomic Force ; Nanofibers ; Shewanella/isolation & purification ; Shewanella/ultrastructure ; Tandem Mass Spectrometry
Sprache	Englisch
Erscheinungsdatum	2011-12
Erscheinungsland	Netherlands
Dokumenttyp	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	604916-3
ISSN	1872-8359 ; 0167-7012
ISSN (online)	1872-8359
ISSN	0167-7012
DOI	10.1016/j.mimet.2011.09.008
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Laser printing of single cells: statistical analysis, cell viability, and stress.

Barron, Jason A / Krizman, David B / Ringeisen, Bradley R

Annals of biomedical engineering

2005 Band 33, Heft 2, Seite(n) 121–130

Abstract: Methods to print patterns of mammalian cells to various substrates with high resolution offer unique possibilities to contribute to a wide range of fields including tissue engineering, cell separation, and functional genomics. This manuscript details ... ...

Abstract	Methods to print patterns of mammalian cells to various substrates with high resolution offer unique possibilities to contribute to a wide range of fields including tissue engineering, cell separation, and functional genomics. This manuscript details experiments demonstrating that BioLP Biological Laser Printing, can be used to rapidly and accurately print patterns of single cells in a noncontact manner. Human osteosarcoma cells were deposited into a biopolymer matrix, and after 6 days of incubation, the printed cells are shown to be 100% viable. Printing low numbers of cells per spot by BioLP is shown to follow a Poisson distribution, indicating that the reproducibility for the number of cells per spot is therefore determined not by the variance in printed volume per drop but by random sampling statistics. Potential cell damage during the laser printing process is also investigated via immunocytochemical studies that demonstrate minimal expression of heat shock proteins by printed cells. Overall, we find that BioLP is able to print patterns of osteosarcoma cells with high viability, little to no heat or shear damage to the cells, and at the ultimate single cell resolution.
Mesh-Begriff(e)	Cell Count/methods ; Cell Culture Techniques/instrumentation ; Cell Culture Techniques/methods ; Cell Line, Tumor ; Cell Proliferation ; Cell Separation/instrumentation ; Cell Separation/methods ; Cell Survival/physiology ; Computer Peripherals ; Heat-Shock Proteins/metabolism ; Heat-Shock Response ; Humans ; Models, Biological ; Models, Statistical ; Osteosarcoma/metabolism ; Osteosarcoma/pathology ; Oxidative Stress/physiology ; Printing/instrumentation ; Printing/methods ; Tissue Engineering/instrumentation ; Tissue Engineering/methods
Chemische Substanzen	Heat-Shock Proteins
Sprache	Englisch
Erscheinungsdatum	2005-03-12
Erscheinungsland	United States
Dokumenttyp	Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	185984-5
ISSN	1573-9686 ; 0090-6964 ; 0191-5649
ISSN (online)	1573-9686
ISSN	0090-6964 ; 0191-5649
DOI	10.1007/s10439-005-8971-x
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Single-cell printing to form three-dimensional lines of olfactory ensheathing cells.

Othon, Christina M / Wu, Xingjia / Anders, Juanita J / Ringeisen, Bradley R

Biomedical materials (Bristol, England)

2008 Band 3, Heft 3, Seite(n) 34101

Abstract: Biological laser printing (BioLP) is a unique tool capable of printing high resolution two- and three-dimensional patterns of living mammalian cells, with greater than 95% viability. These results have been extended to primary cultured olfactory ... ...

Abstract	Biological laser printing (BioLP) is a unique tool capable of printing high resolution two- and three-dimensional patterns of living mammalian cells, with greater than 95% viability. These results have been extended to primary cultured olfactory ensheathing cells (OECs), harvested from adult Sprague-Dawley rats. OECs have been found to provide stimulating environments for neurite outgrowth in spinal cord injury models. BioLP is unique in that small load volumes ( approximately microLs) are required to achieve printing, enabling low numbers of OECs to be harvested, concentrated and printed. BioLP was used to form several 8 mm lines of OECs throughout a multilayer hydrogel scaffold. The line width was as low as 20 microm, with most lines comprising aligned single cells. Fluorescent confocal microscopy was used to determine the functionality of the printed OECs, to monitor interactions between printed OECs, and to determine the extent of cell migration throughout the 3D scaffold. High-resolution printing of low cell count, harvested OECs is an important advancement for in vitro study of cell interactions and functionality. In addition, these cell-printed scaffolds may provide an alternative for spinal cord repair studies, as the single-cell patterns formed here are on relevant size scales for neurite outgrowth.
Mesh-Begriff(e)	Animals ; Cell Culture Techniques/instrumentation ; Cell Culture Techniques/methods ; Cells, Cultured ; Lasers ; Olfactory Bulb/cytology ; Olfactory Bulb/physiology ; Printing/methods ; Rats ; Sensory Receptor Cells/cytology ; Sensory Receptor Cells/physiology ; Tissue Engineering/instrumentation ; Tissue Engineering/methods
Sprache	Englisch
Erscheinungsdatum	2008-09
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2265222-X
ISSN	1748-605X ; 1748-6041
ISSN (online)	1748-605X
ISSN	1748-6041
DOI	10.1088/1748-6041/3/3/034101
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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