Article ; Online: A Versatile Protocol to Quantify BCR-mediated Phosphorylation in Human and Murine B Cell Subpopulations.
2021 Volume 11, Issue 3, Page(s) e3902
Abstract: Signal transduction is the process by which molecular signals are transmitted from the cell surface to its interior, resulting in functional changes inside the cell. B cell receptor (BCR) signaling is of crucial importance for B cells, as it regulates ... ...
| Abstract | Signal transduction is the process by which molecular signals are transmitted from the cell surface to its interior, resulting in functional changes inside the cell. B cell receptor (BCR) signaling is of crucial importance for B cells, as it regulates their differentiation, selection, survival, cellular activation and proliferation. Upon BCR engagement by antigen several protein kinases, lipases and linker molecules become phosphorylated. Phosphoflow cytometry (phosphoflow) is a flow cytometry-based method allowing for analysis of protein phosphorylation in single cells. Due to recent advances in methodology and antibody availability - together with the relatively easy quantification of phosphorylation - phosphoflow is increasingly and more commonly used, compared to classical western blot analysis. It can however be challenging to set-up a method that works for all targets of interest. Here, we present a step-by-step phosphoflow protocol allowing the evaluation of the phosphorylation status of signaling molecules in conjunction with extensive staining to identify various human and murine B cell subpopulations, as was previously published in the original paper by Rip |
|---|---|
| Language | English |
| Publishing date | 2021-02-05 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2833269-6 |
| ISSN | 2331-8325 ; 2331-8325 |
| ISSN (online) | 2331-8325 |
| ISSN | 2331-8325 |
| DOI | 10.21769/BioProtoc.3902 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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