LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 95

Search options

  1. Article ; Online: Development and testing of a sedation protocol for Neocaridina davidi.

    Rodríguez, Diego / Moscoso, Miguel / Desco, Manuel / Ripoll, Jorge / Fernández, Roberto

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 9536

    Abstract: Neocaridina davidi, a small freshwater shrimp native to Asia, specifically China, Japan, Korea, and Vietnam, possesses remarkable resistance to poor water quality and offers various advantages over other invertebrate species to examine crucial issues in ... ...

    Abstract Neocaridina davidi, a small freshwater shrimp native to Asia, specifically China, Japan, Korea, and Vietnam, possesses remarkable resistance to poor water quality and offers various advantages over other invertebrate species to examine crucial issues in neuroscience and other related areas. These advantages include robustness, ease of maintenance, and transparency, making them useful for in vivo studies with optical imaging techniques. Despite its suitability for research purposes, particularly in the fields of imaging and fluorescent techniques, the lack of attention given to this species has resulted in the absence of a robust and replicable sedation protocol for immobilization and safe manipulation. Consequently, researchers face challenges in performing experimental procedures while minimizing harm to this specimen. In this study, we have developed and evaluated a simple sedation protocol specifically designed for Neocaridina davidi, assessing its effectiveness using light microscopy and image processing.
    MeSH term(s) Animals ; Decapoda
    Language English
    Publishing date 2024-04-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-60158-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Derivation of the scalar radiative transfer equation from energy conservation of Maxwell's equations in the far field.

    Ripoll, Jorge

    Journal of the Optical Society of America. A, Optics, image science, and vision

    2011  Volume 28, Issue 8, Page(s) 1765–1775

    Abstract: In this paper the expression for the radiative transfer equation (RTE) commonly used when describing light propagation in biological tissues is derived directly from the equation of energy conservation of Maxwell's equations (Poynting's theorem) by ... ...

    Abstract In this paper the expression for the radiative transfer equation (RTE) commonly used when describing light propagation in biological tissues is derived directly from the equation of energy conservation of Maxwell's equations (Poynting's theorem) by making use of a volume-averaged expression for the time-averaged flow of energy. The derivation is presented step by step with Maxwell's equations as the starting point, analyzing all approximations taken in order to arrive at the expression of the scalar RTE employed in biomedical applications, which neglects particle nonsphericity and orientation, depolarization, and coherence effects.
    MeSH term(s) Absorption ; Biophysics/methods ; Diffusion ; Electricity ; Electromagnetic Phenomena ; Energy Transfer ; Humans ; Models, Statistical ; Monte Carlo Method ; Optics and Photonics ; Surface Properties
    Language English
    Publishing date 2011-08-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 283633-6
    ISSN 1520-8532 ; 1084-7529 ; 0740-3232
    ISSN (online) 1520-8532
    ISSN 1084-7529 ; 0740-3232
    DOI 10.1364/JOSAA.28.001765
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2689: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Hybrid Fourier-real space method for diffuse optical tomography.

    Ripoll, Jorge

    Optics letters

    2010  Volume 35, Issue 5, Page(s) 688–690

    Abstract: Optical tomography has recently witnessed a substantial increase in the size of the data sets used, mainly owing to the use of CCD cameras. Larger data sets render 3D reconstructions more robust, quantitative, and reproducible, but also significantly ... ...

    Abstract Optical tomography has recently witnessed a substantial increase in the size of the data sets used, mainly owing to the use of CCD cameras. Larger data sets render 3D reconstructions more robust, quantitative, and reproducible, but also significantly increase the computing time needed to generate the reconstructed data. Approaches working with spatial-frequencies instead of real space variables seem to be the method of choice in this case, and a direct inversion method that can produce three-dimensional images from very large detector numbers (>10(5)) using either very large source numbers (>10(3)) [Phys. Rev. E 64, 035601 (2001)] or structured illumination [Opt. Lett. 34, 983 (2009)] has been presented. However, most small animal imaging setups typically incur a practical upper limit of only approximately 10(2) sources mainly due to imaging time constraints, and currently all relying on point source illumination. In this Letter, what we believe to be a new approach, which combines Fourier and real space functions, is shown, which fills the gap between traditional fiber-based small data sets that are solved in real space and the very large data sets solved entirely in spatial-frequency domain.
    MeSH term(s) Algorithms ; Fourier Analysis ; Image Enhancement/methods ; Image Interpretation, Computer-Assisted/methods ; Imaging, Three-Dimensional/methods ; Phantoms, Imaging ; Reproducibility of Results ; Sensitivity and Specificity ; Tomography, Optical/instrumentation ; Tomography, Optical/methods
    Language English
    Publishing date 2010-03-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1539-4794
    ISSN (online) 1539-4794
    DOI 10.1364/OL.35.000688
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.

    Gómez-Gaviro, Maria Victoria / Sanderson, Daniel / Ripoll, Jorge / Desco, Manuel

    iScience

    2020  Volume 23, Issue 8, Page(s) 101432

    Abstract: Three-dimensional (3D) optical imaging techniques can expand our knowledge about physiological and pathological processes that cannot be fully understood with 2D approaches. Standard diagnostic tests frequently are not sufficient to unequivocally ... ...

    Abstract Three-dimensional (3D) optical imaging techniques can expand our knowledge about physiological and pathological processes that cannot be fully understood with 2D approaches. Standard diagnostic tests frequently are not sufficient to unequivocally determine the presence of a pathological condition. Whole-organ optical imaging requires tissue transparency, which can be achieved by using tissue clearing procedures enabling deeper image acquisition and therefore making possible the analysis of large-scale biological tissue samples. Here, we review currently available clearing agents, methods, and their application in imaging of physiological or pathological conditions in different animal and human organs. We also compare different optical tissue clearing methods discussing their advantages and disadvantages and review the use of different 3D imaging techniques for the visualization and image acquisition of cleared tissues. The use of optical tissue clearing resources for large-scale biological tissues 3D imaging paves the way for future applications in translational and clinical research.
    Language English
    Publishing date 2020-08-02
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2589-0042
    ISSN (online) 2589-0042
    DOI 10.1016/j.isci.2020.101432
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article: Non-invasive visualization of amyloid-beta deposits in Alzheimer amyloidosis mice using magnetic resonance imaging and fluorescence molecular tomography.

    Ren, Wuwei / Li, Linlin / Zhang, Jianru / Vaas, Markus / Klohs, Jan / Ripoll, Jorge / Wolf, Martin / Ni, Ruiqing / Rudin, Markus

    Biomedical optics express

    2022  Volume 13, Issue 7, Page(s) 3809–3822

    Abstract: Abnormal cerebral accumulation of amyloid-beta peptide (Aβ) is a major hallmark of Alzheimer's disease. Non-invasive monitoring of Aβ deposits enables assessing the disease burden in patients and animal models mimicking aspects of the human disease as ... ...

    Abstract Abnormal cerebral accumulation of amyloid-beta peptide (Aβ) is a major hallmark of Alzheimer's disease. Non-invasive monitoring of Aβ deposits enables assessing the disease burden in patients and animal models mimicking aspects of the human disease as well as evaluating the efficacy of Aβ-modulating therapies. Previous
    Language English
    Publishing date 2022-06-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2572216-5
    ISSN 2156-7085
    ISSN 2156-7085
    DOI 10.1364/BOE.458290
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article: Advances in optical imaging for pharmacological studies.

    Arranz, Alicia / Ripoll, Jorge

    Frontiers in pharmacology

    2015  Volume 6, Page(s) 189

    Abstract: Imaging approaches are an essential tool for following up over time representative parameters of in vivo models, providing useful information in pharmacological studies. Main advantages of optical imaging approaches compared to other imaging methods are ... ...

    Abstract Imaging approaches are an essential tool for following up over time representative parameters of in vivo models, providing useful information in pharmacological studies. Main advantages of optical imaging approaches compared to other imaging methods are their safety, straight-forward use and cost-effectiveness. A main drawback, however, is having to deal with the presence of high scattering and high absorption in living tissues. Depending on how these issues are addressed, three different modalities can be differentiated: planar imaging (including fluorescence and bioluminescence in vivo imaging), optical tomography, and optoacoustic approaches. In this review we describe the latest advances in optical in vivo imaging with pharmacological applications, with special focus on the development of new optical imaging probes in order to overcome the strong absorption introduced by different tissue components, especially hemoglobin, and the development of multimodal imaging systems in order to overcome the resolution limitations imposed by scattering.
    Language English
    Publishing date 2015-09-11
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2015.00189
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Smart Toolkit for Fluorescence Tomography: Simulation, Reconstruction, and Validation.

    Ren, Wuwei / Isler, Helene / Wolf, Martin / Ripoll, Jorge / Rudin, Markus

    IEEE transactions on bio-medical engineering

    2019  Volume 67, Issue 1, Page(s) 16–26

    Abstract: Objective: Fluorescence molecular tomography (FMT) can provide valuable molecular information by mapping the bio-distribution of fluorescent reporter molecules in the intact organism. Various prototype FMT systems have been introduced during the past ... ...

    Abstract Objective: Fluorescence molecular tomography (FMT) can provide valuable molecular information by mapping the bio-distribution of fluorescent reporter molecules in the intact organism. Various prototype FMT systems have been introduced during the past decade. However, none of them has evolved as a standard tool for routine biomedical research. The goal of this paper is to develop a software package that can automate the complete FMT reconstruction procedure.
    Methods: We present smart toolkit for fluorescence tomography (STIFT), a comprehensive platform comprising three major protocols: 1) virtual FMT, i.e., forward modeling and reconstruction of simulated data; 2) control of actual FMT data acquisition; and 3) reconstruction of experimental FMT data.
    Results: Both simulation and phantom experiments have shown robust reconstruction results for homogeneous and heterogeneous tissue-mimicking phantoms containing fluorescent inclusions.
    Conclusion: STIFT can be used for optimization of FMT experiments, in particular for optimizing illumination patterns.
    Significance: This paper facilitates FMT experiments by bridging the gaps between simulation, actual experiments, and data reconstruction.
    MeSH term(s) Algorithms ; Animals ; Computer Simulation ; Female ; Image Processing, Computer-Assisted/methods ; Mice ; Mice, Inbred BALB C ; Optical Imaging ; Phantoms, Imaging ; Reproducibility of Results ; Tomography, Optical/methods
    Language English
    Publishing date 2019-04-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 160429-6
    ISSN 1558-2531 ; 0018-9294
    ISSN (online) 1558-2531
    ISSN 0018-9294
    DOI 10.1109/TBME.2019.2907460
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 425: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Plenoptic projection fluorescence tomography.

    Iglesias, Ignacio / Ripoll, Jorge

    Optics express

    2014  Volume 22, Issue 19, Page(s) 23215–23225

    Abstract: A new method to obtain the three-dimensional localization of fluorochrome distributions in micrometric samples is presented. It uses a microlens array coupled to the image port of a standard microscope to obtain tomographic data by a filtered back- ... ...

    Abstract A new method to obtain the three-dimensional localization of fluorochrome distributions in micrometric samples is presented. It uses a microlens array coupled to the image port of a standard microscope to obtain tomographic data by a filtered back-projection algorithm. Scanning of the microlens array is proposed to obtain a dense data set for reconstruction. Simulation and experimental results are shown and the implications of this approach in fast 3D imaging are discussed.
    MeSH term(s) Algorithms ; Fluorescence ; Imaging, Three-Dimensional/methods ; Phantoms, Imaging ; Reproducibility of Results ; Tomography/instrumentation
    Language English
    Publishing date 2014-09-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1491859-6
    ISSN 1094-4087 ; 1094-4087
    ISSN (online) 1094-4087
    ISSN 1094-4087
    DOI 10.1364/OE.22.023215
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article ; Online: Fluorescence Diffusion in the Presence of Optically Clear Tissues in a Mouse Head Model.

    Ancora, Daniele / Zacharopoulos, Athanasios / Ripoll, Jorge / Zacharakis, Giannis

    IEEE transactions on medical imaging

    2017  Volume 36, Issue 5, Page(s) 1086–1093

    Abstract: Diffuse Optical Tomography commonly neglects or assumes as insignificant the presence of optically clear regions in biological tissues, estimating their contribution as a small perturbation to light transport. The inaccuracy introduced by this practice ... ...

    Abstract Diffuse Optical Tomography commonly neglects or assumes as insignificant the presence of optically clear regions in biological tissues, estimating their contribution as a small perturbation to light transport. The inaccuracy introduced by this practice is examined in detail in the context of a complete, based on realistic geometry, virtual fluorescence Diffuse Optical Tomography experiment where a mouse head is imaged in the presence of cerebral spinal fluid. Despite the small thickness of such layer, we point out that an error is introduced when neglecting it from the model with possibly reduction in the accuracy of the reconstruction and localization of the fluorescence distribution within the brain. The results obtained in the extensive study presented here suggest that fluorescence diffuse neuroimaging studies can be improved in terms of quantitative and qualitative reconstruction by accurately taking into account optically transparent regions especially in the cases where the reconstruction is aided by the prior knowledge of the structural geometry of the specimen. Thus, this has only recently become an affordable choice, thanks to novel computation paradigms that allow to run Monte Carlo photon propagation on a simple graphic card, hence speeding up the process a thousand folds compared to CPU-based solutions.
    Language English
    Publishing date 2017-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 622531-7
    ISSN 1558-254X ; 0278-0062
    ISSN (online) 1558-254X
    ISSN 0278-0062
    DOI 10.1109/TMI.2016.2646518
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2546: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Single Plane Illumination Microscopy for Microfluidic Device Imaging.

    Gomez-Cruz, Clara / Laguna, Sonia / Bachiller-Pulido, Ariadna / Quilez, Cristina / Cañadas-Ortega, Marina / Albert-Smet, Ignacio / Ripoll, Jorge / Muñoz-Barrutia, Arrate

    Biosensors

    2022  Volume 12, Issue 12

    Abstract: Three-dimensional imaging of live processes at a cellular level is a challenging task. It requires high-speed acquisition capabilities, low phototoxicity, and low mechanical disturbances. Three-dimensional imaging in microfluidic devices poses additional ...

    Abstract Three-dimensional imaging of live processes at a cellular level is a challenging task. It requires high-speed acquisition capabilities, low phototoxicity, and low mechanical disturbances. Three-dimensional imaging in microfluidic devices poses additional challenges as a deep penetration of the light source is required, along with a stationary setting, so the flows are not perturbed. Different types of fluorescence microscopy techniques have been used to address these limitations; particularly, confocal microscopy and light sheet fluorescence microscopy (LSFM). This manuscript proposes a novel architecture of a type of LSFM, single-plane illumination microscopy (SPIM). This custom-made microscope includes two mirror galvanometers to scan the sample vertically and reduce shadowing artifacts while avoiding unnecessary movement. In addition, two electro-tunable lenses fine-tune the focus position and reduce the scattering caused by the microfluidic devices. The microscope has been fully set up and characterized, achieving a resolution of 1.50 μm in the x-y plane and 7.93 μm in the z-direction. The proposed architecture has risen to the challenges posed when imaging microfluidic devices and live processes, as it can successfully acquire 3D volumetric images together with time-lapse recordings, and it is thus a suitable microscopic technique for live tracking miniaturized tissue and disease models.
    Language English
    Publishing date 2022-12-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios12121110
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top