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  1. AU="Rita Sarkis"
  2. AU="Ghafouri, Mahsa"
  3. AU=Sanchez-Velazquez Alba AU=Sanchez-Velazquez Alba
  4. AU="Okuni, Noriko"
  5. AU="Katayama, Yuki"
  6. AU="Sarvazyan, Noune"
  7. AU="Mejaški-Bošnjak, Vlatka"
  8. AU="Solaru, Khendi White"
  9. AU="E. Priolo"
  10. AU="Lankelma, Jacqueline M"
  11. AU=Bryan Phillips-Farfn
  12. AU="Dwyer, Sheila E."
  13. AU="Fuentes, Gabriela Cárdenas"
  14. AU="Supekar, Mrudul V"
  15. AU=Benson M T AU=Benson M T
  16. AU="Chrysos, E"
  17. AU="Zhihong Long"
  18. AU=Stratil J
  19. AU="Furse, Cynthia M"
  20. AU="Andres, S. Bernet" AU="Andres, S. Bernet"
  21. AU="Lee, Sang Mok"
  22. AU="Wang, Fei-Yao"
  23. AU="Kanta, Josephine M"
  24. AU="Hatfield, Disa L"
  25. AU="Kalima, Pota"
  26. AU="Charisi, Martha"
  27. AU="Carrie L. Byington"
  28. AU="Weakland, Leo F"
  29. AU="Johannes Müller"
  30. AU="Yu, Jieh-Juen"

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  1. Artikel ; Online: Urtica dioica Leaf Infusion Enhances the Sensitivity of Triple-Negative Breast Cancer Cells to Cisplatin Treatment

    Guy Nafeh / Maria Abi Akl / Jad Samarani / Rawane Bahous / Georges Al Kari / Maria Younes / Rita Sarkis / Sandra Rizk

    Pharmaceuticals, Vol 16, Iss 780, p

    2023  Band 780

    Abstract: Urtica dioica (UD) has been widely used in traditional medicine due to its therapeutic benefits, including its anticancer effects. Natural compounds have a promising potential when used in combination with chemotherapeutic drugs. The present study ... ...

    Abstract Urtica dioica (UD) has been widely used in traditional medicine due to its therapeutic benefits, including its anticancer effects. Natural compounds have a promising potential when used in combination with chemotherapeutic drugs. The present study explores the anticancer and anti-proliferative properties of UD tea in combination with cisplatin on MDA-MB-231 breast cancer cells in vitro. To elucidate the effect of this combination, a cell viability assay, Annexin V/PI dual staining, cell death ELISA, and Western blots were performed. The results showed that the combination of UD and cisplatin significantly decreased the proliferation of MDA-MB-231 cells in a dose- and time-dependent manner compared to each treatment alone. This was accompanied by an increase in two major hallmarks of apoptosis, the flipping of phosphatidylserine to the outer membrane leaflet and DNA fragmentation, as revealed by Annexin V/PI staining and cell death ELISA, respectively. DNA damage was also validated by the upregulation of the cleaved PARP protein as revealed by Western blot analysis. Finally, the increase in the Bax/Bcl-2 ratio further supported the apoptotic mechanism of death induced by this combination. Thus, a leaf infusion of Urtica dioica enhanced the sensitivity of an aggressive breast cancer cell line to cisplatin via the activation of apoptosis.
    Schlagwörter Urtica dioica ; breast cancer ; apoptosis ; cisplatin ; combination therapy ; Medicine ; R ; Pharmacy and materia medica ; RS1-441
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2023-05-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: The selective anti-proliferative and pro-apoptotic effect of A. cherimola on MDA-MB-231 breast cancer cell line

    Maria Younes / Carl Ammoury / Tony Haykal / Leah Nasr / Rita Sarkis / Sandra Rizk

    BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-

    2020  Band 10

    Abstract: Abstract Background Herbal medicines have been a major target for numerous studies through the past years as an alternative treatment for cancer, mainly due to their minimal effects on normal healthy cells. Annona cherimola, popularly known as Cherimoya, ...

    Abstract Abstract Background Herbal medicines have been a major target for numerous studies through the past years as an alternative treatment for cancer, mainly due to their minimal effects on normal healthy cells. Annona cherimola, popularly known as Cherimoya, is an edible natural fruit rich in phytochemical components and known to possess various biological activities. Previous studies have reported the anti-cancerous effect of A. cherimola ethanolic leaf extract (AELE) on leukemia. This study aims at studying the potential anti-cancer activity of this extract in vitro in two different breast cancer cell lines, namely MDA-MB-231 and MCF-7, in addition to investigating its toxicity on normal mesenchymal stem cells. Methods The anti-proliferative effect of AELE was evaluated via cell viability assay. Propidium iodide staining, Cell Death Detection ELISA and flow cytometry analysis of Annexin V binding were used to assess cell cycle progression, DNA fragmentation and apoptosis induction, respectively. Protein expression was determined via Western Blot analysis to decipher the underlying apoptotic molecular mechanism induced upon AELE treatment. Results The anti-proliferative effect of the extract was found to be selective on the triple-negative breast cancer cell line (MDA-MB-231) in a time- and dose-dependent manner with an IC50 of 390.2 μg/mL at 48 h, with no cytotoxic effects on normal murine mesenchymal stem cells. The pro-apoptotic effect was confirmed by the increase in cellular and DNA fragmentation, flipping of the phosphatidylserine moiety to the outer leaflet, and the increase in Annexin V binding. The underlying molecular mechanism revealed the involvement of the mitochondrial pathway, as shown by alterations in mitochondrial permeability and the upregulation of cytochrome c expression. Conclusion All the data presented in our study suggest that AELE exhibits a selective anti-proliferative and pro-apoptotic effect on the chemo-resistant MDA-MB-231 breast cancer cells, providing evidence for the anti-tumor ...
    Schlagwörter Annona cherimola ; Apoptosis ; Breast Cancer ; MDA-MB-231 ; Medicinal foods ; Other systems of medicine ; RZ201-999
    Thema/Rubrik (Code) 616
    Sprache Englisch
    Erscheinungsdatum 2020-11-01T00:00:00Z
    Verlag BMC
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells

    Tony Haykal / Peter Nasr / Mohammad H. Hodroj / Robin I. Taleb / Rita Sarkis / Marvy Nadine El. Moujabber / Sandra Rizk

    Toxins, Vol 11, Iss 9, p

    2019  Band 506

    Abstract: Annona cherimola Mill is a large green fruit with black seeds widely known to possess toxic properties due to the presence of Annonaceous acetogenins. The present study investigates the anti-cancer properties of an Annona cherimola Mill ethanolic seed ... ...

    Abstract Annona cherimola Mill is a large green fruit with black seeds widely known to possess toxic properties due to the presence of Annonaceous acetogenins. The present study investigates the anti-cancer properties of an Annona cherimola Mill ethanolic seed extract on Acute Myeloid Leukemia (AML) cell lines in vitro and elucidates the underlying cellular mechanism. The anti-proliferative effects of the extract on various AML cell lines and normal mesenchymal cells (MSCs) were assessed using WST-1 viability reagent. The pro-apoptotic effect of the extract was evaluated using Annexin V/PI staining and Cell Death ELISA. The underlying mechanism was deciphered by analyzing the expression of various proteins using western blots. Treatment with an A. cherimola seed ethanolic extract promotes a dose- and time-dependent inhibition of the proliferation of various AML cell lines, but not MSCs. Positive Annexin V staining, as well as DNA fragmentation, confirm an increase in apoptotic cell death by upregulating the expression of pro-apoptotic proteins which control both intrinsic and extrinsic pathways of apoptosis. GC/MS analysis revealed the presence of phytosterols, in addition to other bioactive compounds. In conclusion, Annona cherimola Mill seed extract, previously known to possess a potent toxic activity, induces apoptosis in AML cell lines by the activation of both the extrinsic and the intrinsic pathways.
    Schlagwörter Annona cherimola Mill ; apoptosis ; cancer ; leukemia ; toxic ; Medicine ; R
    Thema/Rubrik (Code) 616
    Sprache Englisch
    Erscheinungsdatum 2019-08-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Non-canonical Hedgehog signaling mediates profibrotic hematopoiesis-stroma crosstalk in myeloproliferative neoplasms

    Jessica E. Pritchard / Juliette E. Pearce / Inge A.M. Snoeren / Stijn N.R. Fuchs / Katrin Götz / Fabian Peisker / Silke Wagner / Adam Benabid / Niklas Lutterbach / Vanessa Klöker / James S. Nagai / Monica T. Hannani / Anna K. Galyga / Ellen Sistemich / Bella Banjanin / Niclas Flosdorf / Eric Bindels / Kathrin Olschok / Katharina Biaesch /
    Nicolas Chatain / Neha Bhagwat / Andrew Dunbar / Rita Sarkis / Olaia Naveiras / Marie-Luise Berres / Steffen Koschmieder / Ross L. Levine / Ivan G. Costa / Hélène F.E. Gleitz / Rafael Kramann / Rebekka K. Schneider

    Cell Reports, Vol 43, Iss 1, Pp 113608- (2024)

    1481  

    Abstract: Summary: The role of hematopoietic Hedgehog signaling in myeloproliferative neoplasms (MPNs) remains incompletely understood despite data suggesting that Hedgehog (Hh) pathway inhibitors have therapeutic activity in patients. We aim to systematically ... ...

    Abstract Summary: The role of hematopoietic Hedgehog signaling in myeloproliferative neoplasms (MPNs) remains incompletely understood despite data suggesting that Hedgehog (Hh) pathway inhibitors have therapeutic activity in patients. We aim to systematically interrogate the role of canonical vs. non-canonical Hh signaling in MPNs. We show that Gli1 protein levels in patient peripheral blood mononuclear cells (PBMCs) mark fibrotic progression and that, in murine MPN models, absence of hematopoietic Gli1, but not Gli2 or Smo, significantly reduces MPN phenotype and fibrosis, indicating that GLI1 in the MPN clone can be activated in a non-canonical fashion. Additionally, we establish that hematopoietic Gli1 has a significant effect on stromal cells, mediated through a druggable MIF-CD74 axis. These data highlight the complex interplay between alterations in the MPN clone and activation of stromal cells and indicate that Gli1 represents a promising therapeutic target in MPNs, particularly that Hh signaling is dispensable for normal hematopoiesis.
    Schlagwörter CP: Stem cell research ; Biology (General) ; QH301-705.5
    Sprache Englisch
    Erscheinungsdatum 2024-01-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

    Carl Ammoury / Maria Younes / Marianne El Khoury / Mohammad H. Hodroj / Tony Haykal / Peter Nasr / Marilyne Sily / Robin I. Taleb / Rita Sarkis / Rana Khalife / Sandra Rizk

    BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-

    2019  Band 10

    Abstract: Abstract Background The edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute ...

    Abstract Abstract Background The edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1). Methods The anti-proliferative effect of A. cherimola ethanolic leaf extract was evaluated via cell viability assay. Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. Extract composition was elucidated by Gas Chromatography-Mass Spectrometry (GC-MS). Results Our results showed that the treatment with A. cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. These effects may be attributed to the presence of terpenes which constitute a large component of the leafy extract, as revealed via GC-MS. Conclusion All the data presented in our study show that the terpene-rich A. cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used.
    Schlagwörter Annona cherimola ; Acute myeloid leukemia ; Apoptosis ; Cancer ; Terpenes ; Other systems of medicine ; RZ201-999
    Thema/Rubrik (Code) 616
    Sprache Englisch
    Erscheinungsdatum 2019-12-01T00:00:00Z
    Verlag BMC
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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