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Article: Revisiting the Specificity of PCR Primers for Diagnostics of Xanthomonas citri pv. citri by Experimental and In Silico Analyses.

Delcourt, Suzy / Vernière, Christian / Boyer, Claudine / Pruvost, Olivier / Hostachy, Bruno / Robène-Soustrade, Isabelle

Plant disease

2019 Volume 97, Issue 3, Page(s) 373–378

Abstract: Asiatic citrus canker disease, caused by Xanthomonas citri pv. citri, seriously impacts citrus production worldwide. Two pathogenic variants, A and A*/ ... ...

Abstract	Asiatic citrus canker disease, caused by Xanthomonas citri pv. citri, seriously impacts citrus production worldwide. Two pathogenic variants, A and A*/A
Language	English
Publishing date	2019-02-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-04-12-0351-RE
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Article: Description and phylogenetic placement of Beauveria hoplocheli sp. nov. used in the biological control of the sugarcane white grub, Hoplochelus marginalis, on Reunion Island

Robène-Soustrade, Isabelle / Jouen, Emmanuel / Pastou, Didier / Payet-Hoarau, Magali / Goble, Tarryn / Linderme, Daphné / Lefeuvre, Pierre / Calmès, Cédric / Reynaud, Bernard / Nibouche, Samuel / Costet, Laurent

Mycologia. 2015 Nov. 1, v. 107, no. 6

2015

Abstract: On Reunion Island successful biological control of the sugarcane white grub Hoplochelus marginalis Fairmaire (Coleoptera: Melolonthidae) has been conducted for decades with strains from the entomopathogenic fungal genus Beauveria (Ascomycota: Hypocreales) ...

Abstract	On Reunion Island successful biological control of the sugarcane white grub Hoplochelus marginalis Fairmaire (Coleoptera: Melolonthidae) has been conducted for decades with strains from the entomopathogenic fungal genus Beauveria (Ascomycota: Hypocreales). A study based on morphological characters combined with a multisequence phylogenetic analysis of genes that encode the translation elongation factor 1-alpha (TEF1), RNA polymerase II largest subunit (RPB1), RNA polymerase II second largest subunit (RPB2) and the Bloc nuc intergenic region was carried out on Beauveria strains isolated on Reunion and Madagascar from H. marginalis. This study revealed that these strains, previously identified as Beauveria brongniartii, did not match that species and are closely related to but still distinct from B. malawiensis strains. Therefore we describe the Reunion Island fungus as the new species B. hoplocheli.
Keywords	Beauveria brongniartii ; Coleoptera ; DNA-directed RNA polymerase ; biological control ; fungi ; intergenic DNA ; new species ; peptide elongation factors ; phylogeny ; sugarcane ; Madagascar ; Reunion
Language	English
Dates of publication	2015-1101
Size	p. 1221-1232.
Publishing place	Taylor & Francis
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	281335-x
ISSN	1557-2536 ; 0027-5514
ISSN (online)	1557-2536
ISSN	0027-5514
DOI	10.3852/14-344
Database	NAL-Catalogue (AGRICOLA)

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Article: Multiplex Nested PCR for Detection of Xanthomonas axonopodis pv. allii from Onion Seeds

Robène-Soustrade, Isabelle / Legrand, Delphine / Gagnevin, Lionel / Chiroleu, Frédéric / Laurent, Annie / Pruvost, Olivier

Applied and environmental microbiology. 2010 May 1, v. 76, no. 9

2010

Abstract: Bacterial blight of onion (BBO) is an emerging disease that is present in many onion-producing areas. The causal agent, Xanthomonas axonopodis pv. allii, is seed transmitted. A reliable and sensitive diagnostic tool for testing seed health is needed. ... ...

Abstract	Bacterial blight of onion (BBO) is an emerging disease that is present in many onion-producing areas. The causal agent, Xanthomonas axonopodis pv. allii, is seed transmitted. A reliable and sensitive diagnostic tool for testing seed health is needed. Detection of X. axonopodis pv. allii was achieved using a multiplex nested PCR assay developed using two randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) sequences corresponding to pilus assembly genes (pilW and pilX) and the avrRxv gene, respectively. The multiplex nested PCR was used with a large collection of X. axonopodis pv. allii strains pathogenic to onion and/or other Allium species isolated in different regions of the world. The internal primers used in the multiplex PCR assay directed amplification for all 86 X. axonopodis pv. allii strains tested, resulting in a 401-bp amplicon, a 444- to 447-bp amplicon, or both amplicons, depending on the strain. No amplification was obtained for 41 unrelated phytopathogenic bacteria and for 14 saprophytic bacteria commonly isolated from onion leaves and seeds. Most Xanthomonas strains also did not produce amplicons, except for nine strains classified in X. axonopodis genetic subgroup 9.1 or 9.2 and not pathogenic to onion. Nevertheless, sequence signatures distinguished most of these strains from X. axonopodis pv. allii. The assay detected X. axonopodis pv. allii in seed lots with contamination levels of 5 x 102 CFU g−1 or higher. The sensitivity threshold of the multiplex nested PCR assay was found to be 1 infected seed in 27,340 seeds. This PCR-based assay should be useful for certifying that commercial seed lots are free of this important seed-borne pathogen.
Keywords	Allium ; Xanthomonas axonopodis ; amplified fragment length polymorphism ; blight ; diagnostic techniques ; emerging diseases ; genes ; leaves ; onions ; plant pathogenic bacteria ; random amplified polymorphic DNA technique ; seeds
Language	English
Dates of publication	2010-0501
Size	p. 2697-2703.
Publishing place	American Society for Microbiology
Document type	Article
Note	Includes references
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/AEM.02697-09
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Multiplex nested PCR for detection of Xanthomonas axonopodis pv. allii from onion seeds.

Robène-Soustrade, Isabelle / Legrand, Delphine / Gagnevin, Lionel / Chiroleu, Frédéric / Laurent, Annie / Pruvost, Olivier

Applied and environmental microbiology

2010 Volume 76, Issue 9, Page(s) 2697–2703

Abstract	Bacterial blight of onion (BBO) is an emerging disease that is present in many onion-producing areas. The causal agent, Xanthomonas axonopodis pv. allii, is seed transmitted. A reliable and sensitive diagnostic tool for testing seed health is needed. Detection of X. axonopodis pv. allii was achieved using a multiplex nested PCR assay developed using two randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) sequences corresponding to pilus assembly genes (pilW and pilX) and the avrRxv gene, respectively. The multiplex nested PCR was used with a large collection of X. axonopodis pv. allii strains pathogenic to onion and/or other Allium species isolated in different regions of the world. The internal primers used in the multiplex PCR assay directed amplification for all 86 X. axonopodis pv. allii strains tested, resulting in a 401-bp amplicon, a 444- to 447-bp amplicon, or both amplicons, depending on the strain. No amplification was obtained for 41 unrelated phytopathogenic bacteria and for 14 saprophytic bacteria commonly isolated from onion leaves and seeds. Most Xanthomonas strains also did not produce amplicons, except for nine strains classified in X. axonopodis genetic subgroup 9.1 or 9.2 and not pathogenic to onion. Nevertheless, sequence signatures distinguished most of these strains from X. axonopodis pv. allii. The assay detected X. axonopodis pv. allii in seed lots with contamination levels of 5 x 10(2) CFU g(-1) or higher. The sensitivity threshold of the multiplex nested PCR assay was found to be 1 infected seed in 27,340 seeds. This PCR-based assay should be useful for certifying that commercial seed lots are free of this important seed-borne pathogen.
MeSH term(s)	Amplified Fragment Length Polymorphism Analysis ; Base Sequence ; DNA Primers ; Molecular Sequence Data ; Onions/microbiology ; Plant Leaves/microbiology ; Polymerase Chain Reaction/methods ; Seeds/microbiology ; Xanthomonas axonopodis/genetics ; Xanthomonas axonopodis/isolation & purification
Chemical Substances	DNA Primers
Language	English
Publishing date	2010-03-05
Publishing country	United States
Document type	Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/AEM.02697-09
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Description and phylogenetic placement of Beauveria hoplocheli sp. nov. used in the biological control of the sugarcane white grub, Hoplochelus marginalis, on Reunion Island.

Mycologia

2015 Volume 107, Issue 6, Page(s) 1221–1232

Abstract	On Reunion Island successful biological control of the sugarcane white grub Hoplochelus marginalis Fairmaire (Coleoptera: Melolonthidae) has been conducted for decades with strains from the entomopathogenic fungal genus Beauveria (Ascomycota: Hypocreales). A study based on morphological characters combined with a multisequence phylogenetic analysis of genes that encode the translation elongation factor 1-alpha (TEF1), RNA polymerase II largest subunit (RPB1), RNA polymerase II second largest subunit (RPB2) and the Bloc nuc intergenic region was carried out on Beauveria strains isolated on Reunion and Madagascar from H. marginalis. This study revealed that these strains, previously identified as Beauveria brongniartii, did not match that species and are closely related to but still distinct from B. malawiensis strains. Therefore we describe the Reunion Island fungus as the new species B. hoplocheli.
MeSH term(s)	Animals ; Antibiosis ; Beauveria/classification ; Beauveria/genetics ; Beauveria/isolation & purification ; Beauveria/physiology ; Coleoptera/microbiology ; Coleoptera/physiology ; France ; Fungal Proteins/genetics ; Islands ; Mycological Typing Techniques ; Peptide Elongation Factor 1/genetics ; Pest Control, Biological ; Phylogeny ; Plant Diseases/parasitology ; Plant Diseases/prevention & control ; Saccharum/parasitology ; Spores, Fungal/classification ; Spores, Fungal/genetics ; Spores, Fungal/growth & development ; Spores, Fungal/isolation & purification
Chemical Substances	Fungal Proteins ; Peptide Elongation Factor 1
Language	English
Publishing date	2015-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	281335-x
ISSN	1557-2536 ; 0027-5514
ISSN (online)	1557-2536
ISSN	0027-5514
DOI	10.3852/14-344
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Article: Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium (Anthurium andreanum) Tissues by Nested PCR

Robène-Soustrade, Isabelle / Laurent, Philippe / Gagnevin, Lionel / Jouen, Emmanuel / Pruvost, Olivier

Applied and environmental microbiology. 2006 Feb., v. 72, no. 2

2006

Abstract: Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of anthurium bacterial blight, requires a sensitive and reliable diagnostic tool. A nested PCR test was developed from a sequence-characterized amplified region marker ... ...

Abstract	Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of anthurium bacterial blight, requires a sensitive and reliable diagnostic tool. A nested PCR test was developed from a sequence-characterized amplified region marker identified by randomly amplified polymorphic DNA PCR for the detection of X. axonopodis pv. dieffenbachiae. Serological and pathogenicity tests were performed concurrently with the nested PCR test with a large collection of X. axonopodis pv. dieffenbachiae strains that were isolated worldwide and are pathogenic to anthurium and/or other aroids. The internal primer pair directed amplification of the expected product (785 bp) for all 70 X. axonopodis pv. dieffenbachiae strains pathogenic to anthurium tested and for isolates originating from syngonium and not pathogenic to anthurium. This finding is consistent with previous studies which indicated that there is a high level of relatedness between strains from anthurium and strains from syngonium. Strains originating from the two host genera can be distinguished by restriction analysis of the amplification product. No amplification product was obtained with 98 strains of unrelated phytopathogenic bacteria or saprophytic bacteria from the anthurium phyllosphere, except for a weak signal obtained for one X. axonopodis pv. allii strain. Nevertheless, restriction enzyme analysis permitted the two pathovars to be distinguished. The detection threshold obtained with pure cultures or plant extracts (10³ CFU ml⁻¹) allowed detection of the pathogen from symptomless contaminated plants. This test could be a useful diagnostic tool for screening propagation stock plant material and for monitoring international movement of X. axonopodis pv. dieffenbachiae.
Keywords	Anthurium andraeanum ; Xanthomonas axonopodis pv. dieffenbachiae ; plant pathogenic bacteria ; bacterial diseases of plants ; microbial detection ; disease diagnosis ; disease detection ; polymerase chain reaction ; blight ; DNA primers ; nucleotide sequences ; random amplified polymorphic DNA technique
Language	English
Dates of publication	2006-02
Size	p. 1072-1078.
Document type	Article
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
Database	NAL-Catalogue (AGRICOLA)

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Article: Specific detection of Xanthomonas axonopodis pv. dieffenbachiae in anthurium (Anthurium andreanum) tissues by nested PCR.

Robène-Soustrade, Isabelle / Laurent, Philippe / Gagnevin, Lionel / Jouen, Emmanuel / Pruvost, Olivier

Applied and environmental microbiology

2006 Volume 72, Issue 2, Page(s) 1072–1078

Abstract	Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of anthurium bacterial blight, requires a sensitive and reliable diagnostic tool. A nested PCR test was developed from a sequence-characterized amplified region marker identified by randomly amplified polymorphic DNA PCR for the detection of X. axonopodis pv. dieffenbachiae. Serological and pathogenicity tests were performed concurrently with the nested PCR test with a large collection of X. axonopodis pv. dieffenbachiae strains that were isolated worldwide and are pathogenic to anthurium and/or other aroids. The internal primer pair directed amplification of the expected product (785 bp) for all 70 X. axonopodis pv. dieffenbachiae strains pathogenic to anthurium tested and for isolates originating from syngonium and not pathogenic to anthurium. This finding is consistent with previous studies which indicated that there is a high level of relatedness between strains from anthurium and strains from syngonium. Strains originating from the two host genera can be distinguished by restriction analysis of the amplification product. No amplification product was obtained with 98 strains of unrelated phytopathogenic bacteria or saprophytic bacteria from the anthurium phyllosphere, except for a weak signal obtained for one X. axonopodis pv. allii strain. Nevertheless, restriction enzyme analysis permitted the two pathovars to be distinguished. The detection threshold obtained with pure cultures or plant extracts (10(3) CFU ml(-1)) allowed detection of the pathogen from symptomless contaminated plants. This test could be a useful diagnostic tool for screening propagation stock plant material and for monitoring international movement of X. axonopodis pv. dieffenbachiae.
MeSH term(s)	Araceae/microbiology ; Base Sequence ; DNA, Bacterial/genetics ; Molecular Sequence Data ; Plant Diseases/microbiology ; Plant Extracts ; Polymerase Chain Reaction/methods ; Polymerase Chain Reaction/statistics & numerical data ; Random Amplified Polymorphic DNA Technique ; Sensitivity and Specificity ; Species Specificity ; Virulence ; Xanthomonas/genetics ; Xanthomonas/isolation & purification ; Xanthomonas/pathogenicity
Chemical Substances	DNA, Bacterial ; Plant Extracts
Language	English
Publishing date	2006-02-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/AEM.72.2.1072-1078.2006
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Article ; Online: Genome sequence of Xanthomonas fuscans subsp. fuscans strain 4834-R reveals that flagellar motility is not a general feature of xanthomonads.

Darrasse, Armelle / Carrère, Sébastien / Barbe, Valérie / Boureau, Tristan / Arrieta-Ortiz, Mario L / Bonneau, Sophie / Briand, Martial / Brin, Chrystelle / Cociancich, Stéphane / Durand, Karine / Fouteau, Stéphanie / Gagnevin, Lionel / Guérin, Fabien / Guy, Endrick / Indiana, Arnaud / Koebnik, Ralf / Lauber, Emmanuelle / Munoz, Alejandra / Noël, Laurent D /

Pieretti, Isabelle / Poussier, Stéphane / Pruvost, Olivier / Robène-Soustrade, Isabelle / Rott, Philippe / Royer, Monique / Serres-Giardi, Laurana / Szurek, Boris / van Sluys, Marie-Anne / Verdier, Valérie / Vernière, Christian / Arlat, Matthieu / Manceau, Charles / Jacques, Marie-Agnès

BMC genomics

2013 Volume 14, Page(s) 761

Abstract: Background: Xanthomonads are plant-associated bacteria responsible for diseases on economically important crops. Xanthomonas fuscans subsp. fuscans (Xff) is one of the causal agents of common bacterial blight of bean. In this study, the complete genome ... ...

Abstract	Background: Xanthomonads are plant-associated bacteria responsible for diseases on economically important crops. Xanthomonas fuscans subsp. fuscans (Xff) is one of the causal agents of common bacterial blight of bean. In this study, the complete genome sequence of strain Xff 4834-R was determined and compared to other Xanthomonas genome sequences. Results: Comparative genomics analyses revealed core characteristics shared between Xff 4834-R and other xanthomonads including chemotaxis elements, two-component systems, TonB-dependent transporters, secretion systems (from T1SS to T6SS) and multiple effectors. For instance a repertoire of 29 Type 3 Effectors (T3Es) with two Transcription Activator-Like Effectors was predicted. Mobile elements were associated with major modifications in the genome structure and gene content in comparison to other Xanthomonas genomes. Notably, a deletion of 33 kbp affects flagellum biosynthesis in Xff 4834-R. The presence of a complete flagellar cluster was assessed in a collection of more than 300 strains representing different species and pathovars of Xanthomonas. Five percent of the tested strains presented a deletion in the flagellar cluster and were non-motile. Moreover, half of the Xff strains isolated from the same epidemic than 4834-R was non-motile and this ratio was conserved in the strains colonizing the next bean seed generations. Conclusions: This work describes the first genome of a Xanthomonas strain pathogenic on bean and reports the existence of non-motile xanthomonads belonging to different species and pathovars. Isolation of such Xff variants from a natural epidemic may suggest that flagellar motility is not a key function for in planta fitness.
MeSH term(s)	Base Sequence ; Evolution, Molecular ; Fabaceae/genetics ; Fabaceae/growth & development ; Fabaceae/microbiology ; Flagella/genetics ; Flagella/physiology ; Genetic Fitness ; Genome, Bacterial ; Phylogeny ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Seeds/genetics ; Seeds/microbiology ; Sequence Analysis, DNA ; Xanthomonas/classification ; Xanthomonas/genetics ; Xanthomonas/pathogenicity
Language	English
Publishing date	2013-11-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2041499-7
ISSN	1471-2164 ; 1471-2164
ISSN (online)	1471-2164
ISSN	1471-2164
DOI	10.1186/1471-2164-14-761
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. Manihotis strain CIO151.

Arrieta-Ortiz, Mario L / Rodríguez-R, Luis M / Pérez-Quintero, Álvaro L / Poulin, Lucie / Díaz, Ana C / Arias Rojas, Nathalia / Trujillo, Cesar / Restrepo Benavides, Mariana / Bart, Rebecca / Boch, Jens / Boureau, Tristan / Darrasse, Armelle / David, Perrine / Dugé de Bernonville, Thomas / Fontanilla, Paula / Gagnevin, Lionel / Guérin, Fabien / Jacques, Marie-Agnès / Lauber, Emmanuelle /

Lefeuvre, Pierre / Medina, Cesar / Medina, Edgar / Montenegro, Nathaly / Muñoz Bodnar, Alejandra / Noël, Laurent D / Ortiz Quiñones, Juan F / Osorio, Daniela / Pardo, Carolina / Patil, Prabhu B / Poussier, Stéphane / Pruvost, Olivier / Robène-Soustrade, Isabelle / Ryan, Robert P / Tabima, Javier / Urrego Morales, Oscar G / Vernière, Christian / Carrere, Sébastien / Verdier, Valérie / Szurek, Boris / Restrepo, Silvia / López, Camilo / Koebnik, Ralf / Bernal, Adriana

PloS one

2013 Volume 8, Issue 11, Page(s) e79704

Abstract: Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of bacterial blight of cassava, which is among the main components of human diet in Africa and South America. Current information about the molecular pathogenicity factors involved in the ... ...

Abstract	Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of bacterial blight of cassava, which is among the main components of human diet in Africa and South America. Current information about the molecular pathogenicity factors involved in the infection process of this organism is limited. Previous studies in other bacteria in this genus suggest that advanced draft genome sequences are valuable resources for molecular studies on their interaction with plants and could provide valuable tools for diagnostics and detection. Here we have generated the first manually annotated high-quality draft genome sequence of Xam strain CIO151. Its genomic structure is similar to that of other xanthomonads, especially Xanthomonas euvesicatoria and Xanthomonas citri pv. citri species. Several putative pathogenicity factors were identified, including type III effectors, cell wall-degrading enzymes and clusters encoding protein secretion systems. Specific characteristics in this genome include changes in the xanthomonadin cluster that could explain the lack of typical yellow color in all strains of this pathovar and the presence of 50 regions in the genome with atypical nucleotide composition. The genome sequence was used to predict and evaluate 22 variable number of tandem repeat (VNTR) loci that were subsequently demonstrated as polymorphic in representative Xam strains. Our results demonstrate that Xanthomonas axonopodis pv. manihotis strain CIO151 possesses ten clusters of pathogenicity factors conserved within the genus Xanthomonas. We report 126 genes that are potentially unique to Xam, as well as potential horizontal transfer events in the history of the genome. The relation of these regions with virulence and pathogenicity could explain several aspects of the biology of this pathogen, including its ability to colonize both vascular and non-vascular tissues of cassava plants. A set of 16 robust, polymorphic VNTR loci will be useful to develop a multi-locus VNTR analysis scheme for epidemiological surveillance of this disease.
MeSH term(s)	Genome, Bacterial/genetics ; Minisatellite Repeats/genetics ; Virulence/genetics ; Xanthomonas axonopodis/genetics ; Xanthomonas axonopodis/pathogenicity
Language	English
Publishing date	2013-11-22
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0079704
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Article: Genome sequence of Xanthomonas fuscans subsp fuscans strain 4834-R reveals that flagellar motility is not a general feature of xanthomonads

Darrasse, Armelle / Carrere, Sebastien / Barbe, Valerie / Boureau, Tristan / Arrieta-Ortiz, Mario L. / Bonneau, Sophie / Briand, Martial / Brin, Chrystelle / Cociancich, Stephane / Durand, Karine / Fouteau, Stephanie / Gagnevin, Lionel / Guerin, Fabien / Guy, Endrick / Indiana, Arnaud / Koebnik, Ralf / Lauber, Emmanuelle / Munoz, Alejandra / Noël, Laurent /

Pieretti, Isabelle / Poussier, Stéphane / Pruvost, Olivier / Robene-Soustrade, Isabelle / Rott, Philippe / Royer, Monique / Serres-giardi, Laurana / Szurek, Boris / van Sluys, Marie-Anne / Verdier, Valerie / Verniere, Christian / Arlat, Matthieu / Manceau, Charles / Jacques, Marie Agnes

BMC Genomics (14), . (2013)

Abstract	Background: Xanthomonads are plant-associated bacteria responsible for diseases on economically important crops. Xanthomonas fuscans subsp. fuscans (Xff) is one of the causal agents of common bacterial blight of bean. In this study, the complete genome sequence of strain Xff 4834-R was determined and compared to other Xanthomonas genome sequences.[br/] Results: Comparative genomics analyses revealed core characteristics shared between Xff 4834-R and other xanthomonads including chemotaxis elements, two-component systems, TonB-dependent transporters, secretion systems (from T1SS to T6SS) and multiple effectors. For instance a repertoire of 29 Type 3 Effectors (T3Es) with two Transcription Activator-Like Effectors was predicted. Mobile elements were associated with major modifications in the genome structure and gene content in comparison to other Xanthomonas genomes. Notably, a deletion of 33 kbp affects flagellum biosynthesis in Xff 4834-R. The presence of a complete flagellar cluster was assessed in a collection of more than 300 strains representing different species and pathovars of Xanthomonas. Five percent of the tested strains presented a deletion in the flagellar cluster and were non-motile. Moreover, half of the Xff strains isolated from the same epidemic than 4834-R was non-motile and this ratio was conserved in the strains colonizing the next bean seed generations.[br/] Conclusions: This work describes the first genome of a Xanthomonas strain pathogenic on bean and reports the existence of non-motile xanthomonads belonging to different species and pathovars. Isolation of such Xff variants from a natural epidemic may suggest that flagellar motility is not a key function for in planta fitness.
Language	English
Document type	Article
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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Inter-library loan at ZB MED

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Kategorien

Inter-library loan at ZB MED