LIVIVO - Search results -

Search results

Result 1 - 10 of total 249

Search options

Book ; Online ; E-Book: Ireland and the Climate Crisis

Robbins, David / Torney, Diarmuid / Brereton, Pat

(Palgrave Studies in Media and Environmental Communication,)

2020

Abstract: This book provides a comprehensive overview of Ireland’s response to the climate crisis. The contributions, written by leading scholars across a range of disciplines in the social sciences, humanities and beyond, shed light on diverse aspects of the ... ...

Author's details	edited by David Robbins, Diarmuid Torney, Pat Brereton
Series title	Palgrave Studies in Media and Environmental Communication,
Abstract	This book provides a comprehensive overview of Ireland’s response to the climate crisis. The contributions, written by leading scholars across a range of disciplines in the social sciences, humanities and beyond, shed light on diverse aspects of the climate crisis, the factors shaping Ireland’s response, and prospects for the future. Long regarded as a ‘climate laggard’, Ireland’s response to the urgent societal challenge of climate change has seen new momentum in recent times. The volume will serve as a key reference point for academics, students, policymakers, and a wide range of stakeholders. It will be of interest to readers within Ireland, as well as further afield, who wish to gain a deeper understanding of the constraints on, and opportunities for, successful climate action in Ireland.
Keywords	Communication in the environmental sciences ; Communication ; Climatology ; Environment ; Ethnology/Europe ; Culture ; Environmental Communication ; Media and Communication ; Climate Sciences ; Environmental Sciences ; European Culture
Subject code	551.69415
Language	English
Size	1 online resource (XXV, 311 p. 7 illus.)
Edition	1st ed. 2020.
Publisher	Springer International Publishing ; Imprint: Palgrave Macmillan
Publishing place	Cham
Document type	Book ; Online ; E-Book
Remark	Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
ISBN	3-030-47587-5 ; 3-030-47586-7 ; 978-3-030-47587-1 ; 978-3-030-47586-4
DOI	10.1007/978-3-030-47587-1
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Media coverage of CPEC in Pakistan: the case of the missing frame

Qusien, Rabia / Robbins, David

Climate and Development. 2023 Jan. 02, v. 15, no. 1 p.30-44

2023

Abstract: When the media cover mega infrastructural and economic development projects, the benefits of the projects in terms of job creation and economic gains are emphasized, while the environmental impacts remain generally unreported. This research article ... ...

Abstract	When the media cover mega infrastructural and economic development projects, the benefits of the projects in terms of job creation and economic gains are emphasized, while the environmental impacts remain generally unreported. This research article examines coverage of the China–Pakistan Economic Corridor (CPEC) in the Pakistani media, with a particular focus on how the project is framed by the media in a developing country. We find that the media in Pakistan foreground economic considerations, the project’s geostrategic implications, the internal challenges to its completion, and its impact on national security. Despite Pakistan’s vulnerability to the impacts of climate change, the media frame the project in terms of its potential economic benefits, while frames which foreground climate or environmental risk are largely absent. We theorize that the coverage may provide an example of ‘diversionary framing’ whereby the attention of audiences is directed away from the environmental consequences of CPEC and towards its economic benefits. Our study adds to our understanding of how the media of developing countries respond to the arrival of a mega infrastructure project, and also to the small but growing literature focused on the Pakistani media.
Keywords	climate ; climate change ; economic development ; infrastructure ; risk ; Pakistan ; mega-project ; CPEC ; BRI ; diversionary reframing
Language	English
Dates of publication	2023-0102
Size	p. 30-44.
Publishing place	Taylor & Francis
Document type	Article ; Online
ISSN	1756-5537
DOI	10.1080/17565529.2022.2052258
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Improved process intermediate stability through the identification and elimination of reactive glycation residues - a monoclonal antibody case study.

Bosley, Allen / Cook, Kimberly / Lin, Shihua / Robbins, David

Bioengineered

2022 Volume 13, Issue 6, Page(s) 14402–14412

Abstract: The manufacturing of therapeutic biologics can result in a heterogeneous population of charge variants, encompassing many quality attributes which could impact activity and pharmacokinetics. Monitoring the relative abundance of these charge variants to ... ...

Abstract	The manufacturing of therapeutic biologics can result in a heterogeneous population of charge variants, encompassing many quality attributes which could impact activity and pharmacokinetics. Monitoring the relative abundance of these charge variants to demonstrate process consistency is an expectation of regulatory agencies. Control of the relative abundance of charge variants is also necessary to ensure product comparability across the product lifecycle. We have observed a significant shift in the relative abundance of charged species, as measured by capillary isoelectric focusing, during clarified cell culture fluid holds for several monoclonal antibodies. This lack of stability requires that the hold time for this process intermediate be significantly curtailed, eliminating manufacturing flexibility. We have identified the cause of this shift in relative abundance of charged species as changes in glycation levels, focused predominantly on three conserved, solvent accessible, lysine residues. Mutants of a model protein were generated that show increased charge state stability can be gained by eliminating these reactive lysines. Further, characterization studies were conducted on these mutants to determine the impact to biological activity and stability of the molecule, with no detrimental effects observed. Incorporating this knowledge into the assessments of candidate drugs could allow for the selection of molecules less susceptible to this product degradation pathway, allowing for greater manufacturing flexibility. This process of identifying and removing reactive lysine residues could be useful in the design of drug candidates with improved charge state stability, across a range of modalities.
MeSH term(s)	Antibodies, Monoclonal/genetics ; Cell Culture Techniques ; Glycosylation ; Lysine
Chemical Substances	Antibodies, Monoclonal ; Lysine (K3Z4F929H6)
Language	English
Publishing date	2022-06-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2737830-5
ISSN	2165-5987 ; 2165-5979
ISSN (online)	2165-5987
ISSN	2165-5979
DOI	10.1080/21655979.2022.2086350
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Understanding the effects of system differences for parameter estimation and scale-up of high throughput chromatographic data

Keller, William R. / Evans, Steven T. / Ferreira, Gisela / Robbins, David / Cramer, Steven M.

Journal of chromatography. 2022 Jan. 04, v. 1661

2022

Abstract: In this paper, we evaluate how employing fraction collection and multistep gradients with RoboColumns® (Repligen, formally Atoll) affects both comparison to benchtop experimental data and column simulation parameter estimation. These operational ... ...

Abstract	In this paper, we evaluate how employing fraction collection and multistep gradients with RoboColumns® (Repligen, formally Atoll) affects both comparison to benchtop experimental data and column simulation parameter estimation. These operational differences arise from the RoboColumn® system (operated on an automated liquid handling device) requiring offline analysis for determination of elution profiles rather than the continuous in-line UV curves obtained with larger scale systems. In addition, multistep gradients are used to model the smooth linear gradients of larger scale systems because sequential injections are used to provide liquid flow. Comparisons of two sets of column simulations was first carried out to demonstrate that fraction collection reduced the first moments of the elution peaks by 1/2 of the fraction volumes. Additional column simulations determined that the effect of a multistep gradient approximation on retention volume was dependent upon the gradient step length. An empirical transformation was then developed to correct the first moments obtained from gradient experimental data using the RoboColumn® system. These corrected values provided a more direct comparison of the experimental data at different scales and resulted in a significant improvement in agreement with results obtained using a 20 mL benchtop column. Linear steric mass-action (SMA) parameters were then estimated using the corrected values and employed to successfully predict the performance of the benchtop system data. Finally, these parameters were demonstrated to be well suited for modeling the RoboColumn® gradient data when properly accounting for multistep gradients and fraction collection. This work continues previous investigations into understanding system differences associated with robotic liquid handling devices and proposes a methodology for properly accounting for operational differences to predict operation at larger scales using conventional chromatography systems.
Keywords	chromatography ; liquids ; robots
Language	English
Dates of publication	2022-0104
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	218139-3
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
DOI	10.1016/j.chroma.2021.462696
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Multiprotein GLI Transcriptional Complexes as Therapeutic Targets in Cancer.

Yang, Fan / Wynn, Daniel T / Shen, Chen / Ayad, Nagi G / Robbins, David J

Life (Basel, Switzerland)

2022 Volume 12, Issue 12

Abstract: The Hedgehog signaling pathway functions in both embryonic development and adult tissue homeostasis. Importantly, its aberrant activation is also implicated in the progression of multiple types of cancer, including basal cell carcinoma and ... ...

Abstract	The Hedgehog signaling pathway functions in both embryonic development and adult tissue homeostasis. Importantly, its aberrant activation is also implicated in the progression of multiple types of cancer, including basal cell carcinoma and medulloblastoma. GLI transcription factors function as the ultimate effectors of the Hedgehog signaling pathway. Their activity is regulated by this signaling cascade via their mRNA expression, protein stability, subcellular localization, and ultimately their transcriptional activity. Further, GLI proteins are also regulated by a variety of non-canonical mechanisms in addition to the canonical Hedgehog pathway. Recently, with an increased understanding of epigenetic gene regulation, novel transcriptional regulators have been identified that interact with GLI proteins in multi-protein complexes to regulate GLI transcriptional activity. Such complexes have added another layer of complexity to the regulation of GLI proteins. Here, we summarize recent work on the regulation of GLI transcriptional activity by these novel protein complexes and describe their relevance to cancer, as such GLI regulators represent alternative and innovative druggable targets in GLI-dependent cancers.
Language	English
Publishing date	2022-11-24
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662250-6
ISSN	2075-1729
ISSN	2075-1729
DOI	10.3390/life12121967
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Electronic Monitoring of Mom's Schedule (eMOMS

Jacobson, Lisette T / Bakhache, Nathalie / Dowling, Jolynn / Okut, Hayrettin / Zackula, Rosey / Robbins, David C / Stern, Judy E / Grainger, David A / Befort, Christie

American journal of health promotion : AJHP

2023 Volume 37, Issue 7, Page(s) 953–963

Abstract: Purpose: To elicit feedback from participants who completed the eMOMS: Study design: Qualitative - Semi-structured, telephone interview guide using open-ended questions.: Setting: Rural Great Plains state, United States.: Participants: Of 26 ... ...

Abstract	Purpose: To elicit feedback from participants who completed the eMOMS Study design: Qualitative - Semi-structured, telephone interview guide using open-ended questions. Setting: Rural Great Plains state, United States. Participants: Of 26 individuals who completed the eMOMS Method: Interviews were completed between October 2020 and May 2021. Audio-recordings were transcribed verbatim and organized in Microsoft 365. Data were analyzed using an exploratory, inductive thematic analysis. Results: Participants' mean age was 27.5 (± 5.4) years and mean pre-pregnancy BMI was 29.5 kg/m Conclusion: Findings suggest participants' perceived value of a lifestyle change program coupled with lactation education and support delivered using social media. Findings inform future studies to further adapt lifestyle change programs.
MeSH term(s)	Female ; Pregnancy ; Humans ; Adult ; COVID-19/prevention & control ; Health Behavior ; Life Style ; Electronics ; Lactation
Language	English
Publishing date	2023-07-18
Publishing country	United States
Document type	Randomized Controlled Trial ; Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	645160-3
ISSN	2168-6602 ; 0890-1171
ISSN (online)	2168-6602
ISSN	0890-1171
DOI	10.1177/08901171231189540
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2531: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Process characterization strategy for a precipitation step for host cell protein reduction.

Prentice, Jessica / Vu, Diemchi / Robbins, David / Ferreira, Gisela

Biotechnology progress

2019 Volume 36, Issue 1, Page(s) e2908

Abstract: Process characterization using QbD approaches has rarely been described for precipitation steps used for impurity removal in biopharmaceutical processes. We propose a two-step approach for process characterization in which the first step focuses on ... ...

Abstract	Process characterization using QbD approaches has rarely been described for precipitation steps used for impurity removal in biopharmaceutical processes. We propose a two-step approach for process characterization in which the first step focuses on product quality and the second focuses on process performance. This approach provides an efficient, streamlined strategy for the characterization of precipitation steps under the Quality by Design paradigm. This strategy is demonstrated by a case study for the characterization of a precipitation using sodium caprylate to reduce host cell proteins (HCP) during a monoclonal antibody purification process. Process parameters were methodically selected through a risk assessment based on prior development data and scientific knowledge described in the literature. The characterization studies used two multivariate blocks to decouple and distinguish the impact of product quality (e.g., measured HCP of the recovered product from the precipitation) and process performance (e.g., step yield). Robustness of the precipitation step was further demonstrated through linkage studies across the overall purification process. HCP levels could be robustly reduced to ≤100 ppm in the drug substance when the precipitation step operated within an operation space of ≤1% (m/v) sodium caprylate, pH 5.0-6.0, and filter flux ≤300 L/m
MeSH term(s)	Animals ; CHO Cells ; Caprylates/chemistry ; Cells, Cultured ; Chemical Precipitation ; Cricetulus ; Humans ; Hydrogen-Ion Concentration ; Immunoglobulin G/chemistry ; Oxidation-Reduction
Chemical Substances	Caprylates ; Immunoglobulin G ; octanoic acid (OBL58JN025)
Language	English
Publishing date	2019-10-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	165657-0
ISSN	1520-6033 ; 8756-7938
ISSN (online)	1520-6033
ISSN	8756-7938
DOI	10.1002/btpr.2908
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 4253: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Nuclear Regulation of Wnt/β-Catenin Signaling: It's a Complex Situation.

Anthony, Christin C / Robbins, David J / Ahmed, Yashi / Lee, Ethan

Genes

2020 Volume 11, Issue 8

Abstract: Wnt signaling is an evolutionarily conserved metazoan cell communication pathway required for proper animal development. Of the myriad of signaling events that have been ascribed to cellular activation by Wnt ligands, the canonical Wnt/β-catenin pathway ... ...

Abstract	Wnt signaling is an evolutionarily conserved metazoan cell communication pathway required for proper animal development. Of the myriad of signaling events that have been ascribed to cellular activation by Wnt ligands, the canonical Wnt/β-catenin pathway has been the most studied and best understood. Misregulation of Wnt/β-catenin signaling has been implicated in developmental defects in the embryo and major diseases in the adult. Despite the latter, no drugs that inhibit the Wnt/β-catenin pathway have been approved by the FDA. In this review, we explore the least understood step in the Wnt/β-catenin pathway-nuclear regulation of Wnt target gene transcription. We initially describe our current understanding of the importation of β-catenin into the nucleus. We then focus on the mechanism of action of the major nuclear proteins implicated in driving gene transcription. Finally, we explore the concept of a nuclear Wnt enhanceosome and propose a modified model that describes the necessary components for the transcription of Wnt target genes.
MeSH term(s)	Active Transport, Cell Nucleus ; Animals ; Cell Nucleus/metabolism ; Gene Expression Regulation, Developmental ; Humans ; Wnt Signaling Pathway ; beta Catenin/metabolism
Chemical Substances	beta Catenin
Language	English
Publishing date	2020-08-04
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes11080886
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Pressure-Flow experiments, packing, and modeling for scale-up of a mixed mode chromatography column for biopharmaceutical manufacturing.

Prentice, Jessica / Evans, Steven T / Robbins, David / Ferreira, Gisela

Journal of chromatography. A

2020 Volume 1625, Page(s) 461117

Abstract: To obtain consistent chromatographic behavior, it is important to develop resin packing methods in accordance with the characteristics of each resin. Resins, particularly those with a significant level of compressibility, require proper knowledge of the ... ...

Abstract	To obtain consistent chromatographic behavior, it is important to develop resin packing methods in accordance with the characteristics of each resin. Resins, particularly those with a significant level of compressibility, require proper knowledge of the packing methodology to ensure scalable performance. The study demonstrates the applicability of pressure-flow modeling based on the Blake-Kozeny equation for cellulose based resins, using the MEP HyperCel (Pall) resin as a case study. This approach enabled the understanding of the appropriate bed compressibility and the determination of the minimum column diameter that can predict bed integrity during commercial manufacturing scale operation. Studies suggested that scale-dependent wall effects become negligible for column diameters exceeding 20 cm. Pressure-flow modeling produced a minimum compression recommendation of 0.206 for the MEP HyperCel resin. Columns with diameters up to 80 cm packed with this bed compression yielded incompressible beds with pressure-flow curves consistent with model predictions. Model parameter (particle diameter, viscosity, porosity) values were then varied to demonstrate how changing operating conditions influence model predictions. This analysis supported the successful troubleshooting of unexpected high pressures at the commercial manufacturing scale using MEP HyperCel resin, further supporting the applicability of this approach.
MeSH term(s)	Antibodies, Monoclonal/isolation & purification ; Biological Products/isolation & purification ; Chromatography, Liquid/methods ; Gels/chemistry ; Porosity ; Pressure ; Viscosity
Chemical Substances	Antibodies, Monoclonal ; Biological Products ; Gels
Keywords	covid19
Language	English
Publishing date	2020-04-22
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1171488-8
ISSN	1873-3778 ; 0021-9673
ISSN (online)	1873-3778
ISSN	0021-9673
DOI	10.1016/j.chroma.2020.461117
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Understanding the effects of system differences for parameter estimation and scale-up of high throughput chromatographic data.

Keller, William R / Evans, Steven T / Ferreira, Gisela / Robbins, David / Cramer, Steven M

Journal of chromatography. A

2021 Volume 1661, Page(s) 462696

Abstract	In this paper, we evaluate how employing fraction collection and multistep gradients with RoboColumns® (Repligen, formally Atoll) affects both comparison to benchtop experimental data and column simulation parameter estimation. These operational differences arise from the RoboColumn® system (operated on an automated liquid handling device) requiring offline analysis for determination of elution profiles rather than the continuous in-line UV curves obtained with larger scale systems. In addition, multistep gradients are used to model the smooth linear gradients of larger scale systems because sequential injections are used to provide liquid flow. Comparisons of two sets of column simulations was first carried out to demonstrate that fraction collection reduced the first moments of the elution peaks by 1/2 of the fraction volumes. Additional column simulations determined that the effect of a multistep gradient approximation on retention volume was dependent upon the gradient step length. An empirical transformation was then developed to correct the first moments obtained from gradient experimental data using the RoboColumn® system. These corrected values provided a more direct comparison of the experimental data at different scales and resulted in a significant improvement in agreement with results obtained using a 20 mL benchtop column. Linear steric mass-action (SMA) parameters were then estimated using the corrected values and employed to successfully predict the performance of the benchtop system data. Finally, these parameters were demonstrated to be well suited for modeling the RoboColumn® gradient data when properly accounting for multistep gradients and fraction collection. This work continues previous investigations into understanding system differences associated with robotic liquid handling devices and proposes a methodology for properly accounting for operational differences to predict operation at larger scales using conventional chromatography systems.
MeSH term(s)	Chromatography ; Computer Simulation
Language	English
Publishing date	2021-11-21
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1171488-8
ISSN	1873-3778 ; 0021-9673
ISSN (online)	1873-3778
ISSN	0021-9673
DOI	10.1016/j.chroma.2021.462696
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

Full text online

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito