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  1. AU="Roberto Castronari"
  2. AU="Malkova, Anna"
  3. AU="Wilden, L"
  4. AU="Hamel, Meriem"
  5. AU=Marim Feride
  6. AU="Zhou, Yiwang"
  7. AU="Spengler Neff, Anet" AU="Spengler Neff, Anet"
  8. AU="Stefan Barth"
  9. AU=Gong P

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  1. Artikel ; Online: Monitoring of indoor bioaerosol for the detection of SARS-CoV-2 in different hospital settings

    Emma Tedeschini / Stefania Pasqualini / Carla Emiliani / Ettore Marini / Alessandro Valecchi / Chiara Laoreti / Stefano Ministrini / Barbara Camilloni / Roberto Castronari / Lucio Patoia / Francesco Merante / Stefano Baglioni / Edoardo De Robertis / Matteo Pirro / Antonella Mencacci / Leonella Pasqualini

    Frontiers in Public Health, Vol

    2023  Band 11

    Abstract: BackgroundSpore Trap is an environmental detection technology, already used in the field of allergology to monitor the presence and composition of potentially inspirable airborne micronic bioparticulate. This device is potentially suitable for ... ...

    Abstract BackgroundSpore Trap is an environmental detection technology, already used in the field of allergology to monitor the presence and composition of potentially inspirable airborne micronic bioparticulate. This device is potentially suitable for environmental monitoring of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in hospital, as well as in other high-risk closed environments. The aim of the present study is to investigate the accuracy of the Spore Trap system in detecting SARS-CoV-2 in indoor bioaerosol of hospital rooms.MethodsThe Spore Trap was placed in hospital rooms hosting patients with documented SARS-CoV-2 infection (n = 36) or, as a negative control, in rooms where patients with documented negativity to a Real-Time Polymerase Chain Reaction molecular test for SARS-CoV-2 were admitted (n = 10). The monitoring of the bioaerosol was carried on for 24 h. Collected samples were analyzed by real-time polymerase chain reaction.ResultsThe estimated sensitivity of the Spore Trap device for detecting SARS-CoV-2 in an indoor environment is 69.4% (95% C.I. 54.3-84.4%), with a specificity of 100%.ConclusionThe Spore Trap technology is effective in detecting airborne SARS-CoV-2 virus with excellent specificity and high sensitivity, when compared to previous reports. The SARS-CoV-2 pandemic scenario has suggested that indoor air quality control will be a priority in future public health management and will certainly need to include an environmental bio-investigation protocol.
    Schlagwörter SARS-CoV-2 ; bioaerosol ; aerobiology ; environmental monitoring ; environmental prevention ; Public aspects of medicine ; RA1-1270
    Thema/Rubrik (Code) 333
    Sprache Englisch
    Erscheinungsdatum 2023-04-01T00:00:00Z
    Verlag Frontiers Media S.A.
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: SARS-CoV2 infection impairs the metabolism and redox function of cellular glutathione

    Desirée Bartolini / Anna Maria Stabile / Sabrina Bastianelli / Daniela Giustarini / Sara Pierucci / Chiara Busti / Carmine Vacca / Anna Gidari / Daniela Francisci / Roberto Castronari / Antonella Mencacci / Manlio Di Cristina / Riccardo Focaia / Samuele Sabbatini / Mario Rende / Antimo Gioiello / Gabriele Cruciani / Ranieri Rossi / Francesco Galli

    Redox Biology, Vol 45, Iss , Pp 102041- (2021)

    2021  

    Abstract: Viral infections sustain their replication cycle promoting a pro-oxidant environment in the host cell. In this context, specific alterations of the levels and homeostatic function of the tripeptide glutathione have been reported to play a causal role in ... ...

    Abstract Viral infections sustain their replication cycle promoting a pro-oxidant environment in the host cell. In this context, specific alterations of the levels and homeostatic function of the tripeptide glutathione have been reported to play a causal role in the pro-oxidant and cytopathic effects (CPE) of the virus. In this study, these aspects were investigated for the first time in SARS-CoV2-infected Vero E6 cells, a reliable and well-characterized in vitro model of this infection. SARS-CoV2 markedly decreased the levels of cellular thiols, essentially lowering the reduced form of glutathione (GSH). Such an important defect occurred early in the CPE process (in the first 24 hpi). Thiol analysis in N-acetyl-Cys (NAC)-treated cells and membrane transporter expression data demonstrated that both a lowered uptake of the GSH biosynthesis precursor Cys and an increased efflux of cellular thiols, could play a role in this context. Increased levels of oxidized glutathione (GSSG) and protein glutathionylation were also observed along with upregulation of the ER stress marker PERK. The antiviral drugs Remdesivir (Rem) and Nelfinavir (Nel) influenced these changes at different levels, essentially confirming the importance or blocking viral replication to prevent GSH depletion in the host cell. Accordingly, Nel, the most potent antiviral in our in vitro study, produced a timely activation of Nrf2 transcription factor and a GSH enhancing response that synergized with NAC to restore GSH levels in the infected cells. Despite poor in vitro antiviral potency and GSH enhancing function, Rem treatment was found to prevent the SARS-CoV2-induced glutathionylation of cellular proteins. In conclusion, SARS-CoV2 infection impairs the metabolism of cellular glutathione. NAC and the antiviral Nel can prevent such defect in vitro.
    Schlagwörter COVID-19 ; SARS-CoV-2 ; Glutathione ; Thiols ; Nrf2 ; Protein glutathionylation ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 570
    Sprache Englisch
    Erscheinungsdatum 2021-09-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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