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  1. Article ; Online: Antiviral, Cytoprotective, and Anti-Inflammatory Effect of

    Rocha, Daniele C P / Sisnande, Tháyna / Gavino-Leopoldino, Daniel / Guimarães-Andrade, Iris Paula / Cruz, Fernanda F / Assunção-Miranda, Iranaia / Mendonça, Simony C / Leitão, Gilda Guimarães / Simas, Rosineide Costa / Mohana-Borges, Ronaldo / Leitão, Suzana Guimarães / Allonso, Diego

    Viruses

    2023  Volume 15, Issue 11

    Abstract: Chikungunya fever, a debilitating disease caused by Chikungunya virus (CHIKV), is characterized by a high fever of sudden onset and an intense arthralgia that impairs individual regular activities. Although most symptoms are self-limited, long-term ... ...

    Abstract Chikungunya fever, a debilitating disease caused by Chikungunya virus (CHIKV), is characterized by a high fever of sudden onset and an intense arthralgia that impairs individual regular activities. Although most symptoms are self-limited, long-term persistent arthralgia is observed in 30-40% of infected individuals. Currently, there is no vaccine or specific treatment against CHIKV infection, so there is an urgent need for the discovery of new therapeutic options for CHIKF chronic cases. This present study aims to test the antiviral, cytoprotective, and anti-inflammatory activities of an ethanol extract (FF72) from
    MeSH term(s) Humans ; Chikungunya Fever/drug therapy ; Wood ; Triterpenes/pharmacology ; Chikungunya virus ; Virus Replication ; Saponins/pharmacology ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Ethanol/pharmacology ; Arthralgia/drug therapy ; Dammaranes
    Chemical Substances Triterpenes ; Saponins ; Antiviral Agents ; Plant Extracts ; Ethanol (3K9958V90M)
    Language English
    Publishing date 2023-11-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15112232
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Increased circulating levels of High Mobility Group Box 1 (HMGB1) in acute-phase Chikungunya virus infection: Potential disease biomarker.

    Rocha, Daniele C P / Souza, Thiara Manuelle Alves / Nunes, Priscila Conrado Guerra / Mohana-Borges, Ronaldo / Paes, Marciano V / Guimarães, Gabriel M C / Arcila, Juan C S / Paiva, Iury Amâncio / Azeredo, Elzinandes Leal de / Damasco, Paulo Vieira / de Souza, Luiz José / Dos Santos, Flavia B / Allonso, Diego

    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

    2021  Volume 146, Page(s) 105054

    Abstract: Background: Chikungunya virus (CHIKV) causes a febrile syndrome with intense and debilitating arthralgia that can persist for several months or years after complete virus clearance. As there is no specific antiviral treatment or vaccine against CHIKV, ... ...

    Abstract Background: Chikungunya virus (CHIKV) causes a febrile syndrome with intense and debilitating arthralgia that can persist for several months or years after complete virus clearance. As there is no specific antiviral treatment or vaccine against CHIKV, identification of serological markers that help clinical management of CHIKV patients is urgent. The High Mobility Group Box 1 (HMGB1) protein is secreted to extracellular milieu and triggers an intense inflammatory process by inducing the overexpression of pro-inflammatory cytokines. HMGB1 plays an important role in several virus diseases as well as in rheumatoid arthritis.
    Objectives: This study focus on the investigation of HMGB1 serum levels in a sera panel from CHIKV-infected patients in an attempt to assess its potential as a biomarker for chikungunya clinical management.
    Study design: Eighty CHIKV-positive samples and 32 samples from healthy donors were subjected to a quantitative HMGB1 ELISA assay to assess the HMGB1 circulating levels.
    Results: HMGB1 levels were significantly higher in CHIKV-positive samples (516.12 ng/mL, SEM ± 48.83 ng/mL) compared to negative control (31.20 ng/mL, SEM ± 3.24 ng/mL, p < 0.0001). Circulating levels of HMGB1 persisted elevated during the whole acute-phase of disease and correlated with virus titer (p < 0.05).
    Conclusions: The present study is the first to describe increased serum levels of HMGB1 in CHIKV infection and its positive correlation with virus titer, suggesting its potential use as a biomarker for diagnosis and treatment of chikungunya fever.
    MeSH term(s) Biomarkers ; Case-Control Studies ; Chikungunya Fever ; Chikungunya virus ; Enzyme-Linked Immunosorbent Assay ; HMGB1 Protein/blood ; Humans
    Chemical Substances Biomarkers ; HMGB1 Protein ; HMGB1 protein, human
    Language English
    Publishing date 2021-12-11
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1446080-4
    ISSN 1873-5967 ; 1386-6532
    ISSN (online) 1873-5967
    ISSN 1386-6532
    DOI 10.1016/j.jcv.2021.105054
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3790: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Dengue Virus NS1 Protein Modulates Cellular Energy Metabolism by Increasing Glyceraldehyde-3-Phosphate Dehydrogenase Activity.

    Allonso, Diego / Andrade, Iamara S / Conde, Jonas N / Coelho, Diego R / Rocha, Daniele C P / da Silva, Manuela L / Ventura, Gustavo T / Silva, Emiliana M / Mohana-Borges, Ronaldo

    Journal of virology

    2015  Volume 89, Issue 23, Page(s) 11871–11883

    Abstract: Unlabelled: Dengue is one of the main public health concerns worldwide. Recent estimates indicate that over 390 million people are infected annually with the dengue virus (DENV), resulting in thousands of deaths. Among the DENV nonstructural proteins, ... ...

    Abstract Unlabelled: Dengue is one of the main public health concerns worldwide. Recent estimates indicate that over 390 million people are infected annually with the dengue virus (DENV), resulting in thousands of deaths. Among the DENV nonstructural proteins, the NS1 protein is the only one whose function during replication is still unknown. NS1 is a 46- to 55-kDa glycoprotein commonly found as both a membrane-associated homodimer and a soluble hexameric barrel-shaped lipoprotein. Despite its role in the pathogenic process, NS1 is essential for proper RNA accumulation and virus production. In the present study, we identified that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) interacts with intracellular NS1. Molecular docking revealed that this interaction occurs through the hydrophobic protrusion of NS1 and the hydrophobic residues located at the opposite side of the catalytic site. Moreover, addition of purified recombinant NS1 enhanced the glycolytic activity of GAPDH in vitro. Interestingly, we observed that DENV infection promoted the relocalization of GAPDH to the perinuclear region, where NS1 is commonly found. Both DENV infection and expression of NS1 itself resulted in increased GAPDH activity. Our findings indicate that the NS1 protein acts to increase glycolytic flux and, consequently, energy production, which is consistent with the recent finding that DENV induces and requires glycolysis for proper replication. This is the first report to propose that NS1 is an important modulator of cellular energy metabolism. The data presented here provide new insights that may be useful for further drug design and the development of alternative antiviral therapies against DENV.
    Importance: Dengue represents a serious public health problem worldwide and is caused by infection with dengue virus (DENV). Estimates indicate that half of the global population is at risk of infection, with almost 400 million cases occurring per year. The NS1 glycoprotein is found in both the intracellular and the extracellular milieus. Despite the fact that NS1 has been commonly associated with DENV pathogenesis, it plays a pivotal but unknown role in the replication process. In an effort to understand the role of intracellular NS1, we demonstrate that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) interacts with NS1. Our results indicate that NS1 increases the glycolytic activity of GAPDH in vitro. Interestingly, the GAPDH activity was increased during DENV infection, and NS1 expression alone was sufficient to enhance intracellular GAPDH activity in BHK-21 cells. Overall, our findings suggest that NS1 is an important modulator of cellular energy metabolism by increasing glycolytic flux.
    MeSH term(s) Analysis of Variance ; Animals ; Cell Line ; Chromatography, Liquid ; Cricetinae ; Energy Metabolism/physiology ; Enzyme-Linked Immunosorbent Assay ; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/metabolism ; Human Umbilical Vein Endothelial Cells ; Humans ; Immunoprecipitation ; Microscopy, Fluorescence ; Models, Molecular ; Protein Binding ; Tandem Mass Spectrometry ; Viral Nonstructural Proteins/chemistry ; Viral Nonstructural Proteins/genetics ; Viral Nonstructural Proteins/metabolism
    Chemical Substances NS1 protein, Dengue virus type 2 ; Viral Nonstructural Proteins ; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) (EC 1.2.1.12)
    Language English
    Publishing date 2015-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.01342-15
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 609: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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