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Article ; Online: Intermediate-Term Clinical Outcomes of High-Density Autologous Chondrocyte Implantation in Patients with Concomitant Anterior Cruciate Ligament Reconstruction and Focal Chondral Lesions.

Guillén-Vicente, Isabel / Herros-García, Ramón / Guillén-Vicente, Marta / Ruiz, Borja / Lopez-Alcorocho, Juan Manuel / Rodríguez-Iñigo, Elena / Barrera, Adrián / Fernández-Jaén, Tomás F / Abelow, Steve / Guillén-García, Pedro

Cartilage

2024 , Page(s) 19476035241247642

Abstract: Objective: To investigate intermediate-term clinical results in patients with concomitant anterior cruciate ligament (ACL) reconstruction and chondral defect treated with high-density autologous chondrocyte implantation (HD-ACI) compared to patients ... ...

Abstract	Objective: To investigate intermediate-term clinical results in patients with concomitant anterior cruciate ligament (ACL) reconstruction and chondral defect treated with high-density autologous chondrocyte implantation (HD-ACI) compared to patients without ACL tear but with a chondral lesion and HD-ACI treatment. Design: Forty-eight patients with focal chondral lesions underwent HD-ACI (24 with ACL reconstruction after an ACL injury and 24 with an intact ACL). Follow-up assessments occurred at 6, 12, and 24 months. Patient-reported knee function and symptoms were assessed using the International Knee Documentation Committee (IKDC) questionnaire, pain was measured using the Visual Analog Scale (VAS), and adverse events were monitored. Physical activity was assessed using the Tegner Activity Level Scale, and cartilage healing was evaluated with the Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) score. Results: No significant adverse events occurred during follow-up. Both groups showed significant improvements at 2 years compared to baseline (VAS: 8.0 ± 1.3 to 1.4 ± 2.0 [normal ACL]; 7.4 ± 2.3 to 2.1 ± 2.3 [ACL reconstruction]; IKDC: 39.2 ± 10.6 to 76.1 ± 22.0 [intact ACL]; 35.6 ± 12.1 to 74.6 ± 20.9 [ACL reconstruction]). Patients in both groups exceeded the minimal clinically important difference (MCID) for IKDC scores. The Tegner Activity Level Scale decreased immediately after surgery and increased after 2 years, with 70.6% (normal ACL) and 89.5% (ACL reconstruction) returning to their preinjury activity levels. No significant differences in the MOCART score were observed between the groups. Conclusions: ACL reconstruction does not appear to reduce the outcomes (at 2 years) of HD-ACI.
Language	English
Publishing date	2024-04-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2515870-3
ISSN	1947-6043 ; 1947-6035
ISSN (online)	1947-6043
ISSN	1947-6035
DOI	10.1177/19476035241247642
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Article: Cartilage Defect Treatment Using High-Density Autologous Chondrocyte Implantation (HD-ACI).

Guillén-García, Pedro / Guillén-Vicente, Isabel / Rodríguez-Iñigo, Elena / Guillén-Vicente, Marta / Fernández-Jaén, Tomás Fernando / Navarro, Ramón / Aboli, Lucía / Torres, Raúl / Abelow, Steve / López-Alcorocho, Juan Manuel

Bioengineering (Basel, Switzerland)

2023 Volume 10, Issue 9

Abstract: Hyaline cartilage's inability to self-repair can lead to osteoarthritis and joint replacement. Various treatments, including cell therapy, have been developed for cartilage damage. Autologous chondrocyte implantation (ACI) is considered the best option ... ...

Abstract	Hyaline cartilage's inability to self-repair can lead to osteoarthritis and joint replacement. Various treatments, including cell therapy, have been developed for cartilage damage. Autologous chondrocyte implantation (ACI) is considered the best option for focal chondral lesions. In this article, we aimed to create a narrative review that highlights the evolution and enhancement of our chondrocyte implantation technique: High-Density-ACI (HD-ACI) Membrane-assisted Autologous Chondrocyte Implantation (MACI) improved ACI using a collagen membrane as a carrier. However, low cell density in MACI resulted in softer regenerated tissue. HD-ACI was developed to improve MACI, implanting 5 million chondrocytes per cm
Language	English
Publishing date	2023-09-13
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2746191-9
ISSN	2306-5354
ISSN	2306-5354
DOI	10.3390/bioengineering10091083
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Article ; Online: High-Density Autologous Chondrocyte Implantation as Treatment for Ankle Osteochondral Defects.

López-Alcorocho, Juan Manuel / Guillén-Vicente, Isabel / Rodríguez-Iñigo, Elena / Navarro, Ramón / Caballero-Santos, Rosa / Guillén-Vicente, Marta / Casqueiro, Mercedes / Fernández-Jaén, Tomás F / Sanz, Fernando / Arauz, Santiago / Abelow, Steve / Guillén-García, Pedro

Cartilage

2019 Volume 12, Issue 3, Page(s) 307–319

Abstract: Purpose: Two-year follow-up to assess efficacy and safety of high-density autologous chondrocyte implantation (HD-ACI) in patients with cartilage lesions in the ankle.: Design: Twenty-four consecutive patients with International Cartilage repair ... ...

Abstract	Purpose: Two-year follow-up to assess efficacy and safety of high-density autologous chondrocyte implantation (HD-ACI) in patients with cartilage lesions in the ankle. Design: Twenty-four consecutive patients with International Cartilage repair Society (ICRS) grade 3-4 cartilage lesions of the ankle were included. Five million chondrocytes per cm Results: Patients' median age was 31 years (range 18-55 years). Median VAS score was 8 (range 5-10) at baseline, 1.5 (range 0-8) at 12-month follow-up, and 2 (rang e0-5) at 24-month follow-up ( Conclusion: HD-ACI is a safe and effective technique to treat osteochondral lesions in the talus, providing good clinical and histological results at short- and mid-term follow-ups.
MeSH term(s)	Adolescent ; Adult ; Ankle ; Ankle Joint/surgery ; Chondrocytes ; Humans ; Intra-Articular Fractures ; Middle Aged ; Talus ; Transplantation, Autologous ; Young Adult
Language	English
Publishing date	2019-03-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2515870-3
ISSN	1947-6043 ; 1947-6035
ISSN (online)	1947-6043
ISSN	1947-6035
DOI	10.1177/1947603519835898
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Article ; Online: Study of Telomere Length in Preimplanted Cultured Chondrocytes.

López-Alcorocho, Juan Manuel / Guillén-Vicente, Isabel / Rodríguez-Iñigo, Elena / Guillén-Vicente, Marta / Fernández-Jaén, Tomás Fernando / Caballero, Rosa / Casqueiro, Mercedes / Najarro, Pilar / Abelow, Steve / Guillén-García, Pedro

Cartilage

2018 Volume 10, Issue 1, Page(s) 36–42

Abstract: Design: In the process of cell division, the extremes of the eukaryotic chromosomes are progressively shortening, and this phenomenon is related to cell degeneration and senescence. The treatment of cartilage lesions with autologous chondrocytes implies ...

Abstract	Design: In the process of cell division, the extremes of the eukaryotic chromosomes are progressively shortening, and this phenomenon is related to cell degeneration and senescence. The treatment of cartilage lesions with autologous chondrocytes implies that cells proliferate in an artificial environment. We have studied the viability of cultured chondrocytes after measurement of their telomere length before implantation. Methods: Articular cartilage biopsies (B1, B2, and B3) were obtained from 3 patients (2 males and 1 female) with knee cartilage defects, who were going to be treated with chondrocyte implantation. Chondrocytes were cultured in DMEM with autologous serum. After the third passage, an aliquot of 1 million cells was removed to estimate the telomere length and the remaining cells were implanted. Telomere length was measured by quantitative fluorescent in situ hybridization (Q-FISH). Patients' clinical outcome was determined preoperatively, and 12 and 24 months postimplantation with the International Knee Documentation Committee (IKDC) questionnaire. Results: After chondrocyte implantation, IKDC score doubled at 12 and 24 months with regard to the basal value. After 3 passages, chondrocytes were cultured for a mean of 45.67 days, the mean duplication time being 4.53 days and the mean number of cell divisions being 10.04 during the culture period. The 20th percentile of telomere lengths were 6.84, 6.96, and 7.06 kbp and the median telomere lengths 10.30, 10.47, and 10.73 kbp, respectively. No significant correlation was found between IKDC score and telomere length. Conclusion: Culturing autologous chondrocytes for implantation is not related to cell senescence in terms of telomere length.
MeSH term(s)	Adult ; Cartilage Diseases/pathology ; Cartilage Diseases/therapy ; Cartilage, Articular/cytology ; Cartilage, Articular/pathology ; Cells, Cultured ; Chondrocytes/pathology ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Knee Joint/cytology ; Knee Joint/pathology ; Male ; Stem Cell Transplantation ; Telomere/pathology ; Transplantation, Autologous
Language	English
Publishing date	2018-01-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2515870-3
ISSN	1947-6043 ; 1947-6035
ISSN (online)	1947-6043
ISSN	1947-6035
DOI	10.1177/1947603517749918
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Article: The Importance of the Intercondylar Notch in Anterior Cruciate Ligament Tears.

Fernández-Jaén, Tomás / López-Alcorocho, Juan Manuel / Rodriguez-Iñigo, Elena / Castellán, Fabián / Hernández, Juan Carlos / Guillén-García, Pedro

Orthopaedic journal of sports medicine

2015 Volume 3, Issue 8, Page(s) 2325967115597882

Abstract: Background: The factors associated with anterior cruciate ligament (ACL) tears are not completely clear. Some studies have shown that patients with a narrow intercondylar notch have a predisposition for ACL tears.: Purpose: To determine the ... ...

Abstract	Background: The factors associated with anterior cruciate ligament (ACL) tears are not completely clear. Some studies have shown that patients with a narrow intercondylar notch have a predisposition for ACL tears. Purpose: To determine the relationship between the α angle and intercondylar notch width measurements and ACL tears. Study design: Case-control study; Level of evidence, 3. Methods: A total of 530 patients (308 with ACL rupture, 222 with healthy ACLs) were included in this study. The α angle and intercondylar width were measured from magnetic resonance images (MRIs). Binary logistic regression analysis was performed to determine the influence of the variables on ACL status (normal or torn). Odds ratios (ORs) and their respective 95% CIs were also calculated. Results: No significant differences in patient age and the affected knee were found between patients with normal or torn ACLs. The mean α angle was higher in patients with a torn ACL than in those with an intact one (57.5° ± 5.5° vs 56.2° ± 4.5°; P = .009). Intercondylar width was significantly lower in patients with a torn ACL than in those with an intact one (18.2 ± 3.1 vs 19.5 ± 3.6 mm; P < .001). A highly significant difference between men and women was found for mean intercondylar notch width (19.3 ± 3.3 vs 17.4 ± 3.1 mm; P < .001). In a logistic regression model, sex, intercondylar width, and α angle were statistically significant when adjusted for age. Conclusion: Study results suggest that the ACL tears are associated with a narrow intercondylar notch and a high α angle, and that tears occur more frequently in men than in women. Clinical relevance: The model proposed in this study could be used by the physician in the medical office as a tool to identify the risk factors that may predispose a patient for a potential ACL tear.
Language	English
Publishing date	2015-08-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2706251-X
ISSN	2325-9671
ISSN	2325-9671
DOI	10.1177/2325967115597882
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Article ; Online: Cartilage Defect Treatment Using High-Density Autologous Chondrocyte Implantation: Two-Year Follow-up.

Lopez-Alcorocho, Juan Manuel / Aboli, Lucia / Guillen-Vicente, Isabel / Rodriguez-Iñigo, Elena / Guillen-Vicente, Marta / Fernández-Jaén, Tomás F / Arauz, Santiago / Abelow, Steve / Guillen-García, Pedro

Cartilage

2017 Volume 9, Issue 4, Page(s) 363–369

Abstract: Objective: The aim of this work was to study the short- and mid-term effectiveness and safety of high-density autologous chondrocyte implantation (HD-ACI) in the first 50 patients with knee cartilage damage treated in our unit.: Design: Fifty ... ...

Abstract	Objective: The aim of this work was to study the short- and mid-term effectiveness and safety of high-density autologous chondrocyte implantation (HD-ACI) in the first 50 patients with knee cartilage damage treated in our unit. Design: Fifty consecutive patients with cartilage lesions (Outerbridge grade III-IV) in the knee treated with HD-ACI were included in this study. Chondrocytes were isolated from a nonbearing cartilage area biopsy and were cultured until 40 to 50 million cells were obtained. Five million chondrocytes per cm Results: The percentage of patients with pain and swelling decreased progressively in the following visits, with differences being statistically significant ( P < 0.001 and P = 0.040, respectively). IKDC scores improved progressively throughout the 24-month follow-up ( P < 0.001). Thus, the mean IKDC score improvement was 26.3 points (95% confidence interval [CI] = 18.2-34.4 points) at 12 months and 31.0 points (95% CI = 22.9-39 points) at 24 months. No significant differences were found when performing extension ( P = 0.112). Flexion significantly improved by 25.1° at 24-month follow-up ( P = 0.013). Conclusions: HD-ACI is a safe and effective technique for the treatment of cartilage defects, improving clinical and subjective perception of knee functionality. These preliminary results encourage future studies comparing this technique with traditional ACI.
MeSH term(s)	Adolescent ; Adult ; Animals ; Arthroplasty, Subchondral/methods ; Cartilage Diseases/surgery ; Cartilage, Articular/surgery ; Chondrocytes/transplantation ; Female ; Follow-Up Studies ; Humans ; Knee Joint/surgery ; Male ; Middle Aged ; Prospective Studies ; Swine ; Transplantation, Autologous ; Treatment Outcome ; Young Adult
Language	English
Publishing date	2017-02-15
Publishing country	United States
Document type	Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2515870-3
ISSN	1947-6043 ; 1947-6035
ISSN (online)	1947-6043
ISSN	1947-6035
DOI	10.1177/1947603517693045
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Article: Viability of Pathologic Cartilage Fragments as a Source for Autologous Chondrocyte Cultures.

Guillén-García, Pedro / Rodríguez-Iñigo, Elena / Guillén-Vicente, Isabel / Guillén-Vicente, Marta / Fernández-Jaén, Tomás / Concejero, Vicente / Val, Daniel / Maestro, Antonio / Abelow, Steve / López-Alcorocho, Juan Manuel

Cartilage

2016 Volume 7, Issue 2, Page(s) 149–156

Abstract: Objective: To study if a culture of chondrocytes can be obtained from pathologic hyaline cartilage (PHC) fragments.: Design: Twenty-five men and 9 women with osteochondritis dissecans (OCD) in 11 cases, arthrosis in 13 patients, and trauma in the ... ...

Abstract	Objective: To study if a culture of chondrocytes can be obtained from pathologic hyaline cartilage (PHC) fragments. Design: Twenty-five men and 9 women with osteochondritis dissecans (OCD) in 11 cases, arthrosis in 13 patients, and trauma in the remaining 10 cases were included. The PHC fragments and a small sample of the next healthy cartilage were extracted by arthroscopy. According to the appearance, the PHC samples were divided into fixed (3 cases), flapped (6 patients), or loose bodies (25 cases), depending on the attachment degree of the cartilage to the subchondral bone. Approximately half of each pathologic sample and the whole healthy one were digested to isolate the cells trying to establish the cell culture. Results: We were able to establish a cell culture in 7 out of 34 (20.6%) PHC samples (positive samples), whereas in the remaining 27 (79.4%) no cell growth was observed (negative samples). Most of the negative samples were loose bodies (P = 0.005) taken from patients with OCD or arthrosis (P = 0.001) with an evolution time of more than 1 year (P < 0.001). The best binary logistic regression model (P < 0.001) showed that the only factor affecting the establishment of cell culture was the evolution time (P = 0.044). Conclusion: It is possible to culture chondrocytes from osteochondral fragments if they are traumatic, within a year of injury and not from fragments due to arthrosis or OCD.
Language	English
Publishing date	2016-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	2515870-3
ISSN	1947-6043 ; 1947-6035
ISSN (online)	1947-6043
ISSN	1947-6035
DOI	10.1177/1947603515621998
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Article: Hepatitis C virus does not infect muscle, the intervertebral disk, or the meniscus in patients with chronic hepatitis C

Bartolomé, Javier / Rodríguez-Iñigo, Elena / Erice, Alejo / Vidal, Sonia / Castillo, Inmaculada / Carreño, Vicente

Journal of medical virology. 2007 Dec., v. 79, no. 12

2007

Abstract: Chronic infection with hepatitis C virus (HCV) is associated with several extrahepatic manifestations, including neuromuscular and joint disorders, and HCV RNA has been detected in muscle fibers of patients with myosistis and chronic hepatitis C. However, ...

Abstract	Chronic infection with hepatitis C virus (HCV) is associated with several extrahepatic manifestations, including neuromuscular and joint disorders, and HCV RNA has been detected in muscle fibers of patients with myosistis and chronic hepatitis C. However, whether HCV infects muscle cells in patients without myosistis is unknown. The presence of HCV in other sites of the musculoskeletal system has not been investigated. In the present study the presence of HCV RNA was sought in muscle (2 cases), intervertebral disk (1 case) and meniscus (1 case) samples from patients with chronic hepatitis C. HCV RNA was not detected by reverse transcription and real-time polymerase chain reaction in any of the samples tested. In conclusion, the results do not support a direct role of HCV in musculoskeletal disorders associated with chronic hepatitis C. J. Med. Virol. 79:1818-1820, 2007. © Wiley-Liss, Inc.
Language	English
Dates of publication	2007-12
Size	p. 1818-1820.
Publishing place	Wiley Subscription Services, Inc., A Wiley Company
Document type	Article
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.21005
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 1320: Show issues

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Article ; Online: αKLOTHO and sTGFβR2 treatment counteract the osteoarthritic phenotype developed in a rat model.

Martinez-Redondo, Paloma / Guillen-Guillen, Isabel / Davidsohn, Noah / Wang, Chao / Prieto, Javier / Kurita, Masakazu / Hatanaka, Fumiyuki / Zhong, Cuiqing / Hernandez-Benitez, Reyna / Hishida, Tomoaki / Lezaki, Takashi / Sakamoto, Akihisa / Nemeth, Amy N / Hishida, Yuriko / Esteban, Concepcion Rodriguez / Shojima, Kensaku / Huang, Ling / Shokhirev, Maxim / Nuñez-Delicado, Estrella /

Campistol, Josep M / Guillen-Vicente, Isabel / Rodriguez-Iñigo, Elena / Lopez-Alcorocho, Juan Manuel / Guillen-Vicente, Marta / Church, George / Reddy, Pradeep / Guillen-Garcia, Pedro / Liu, Guang-Hui / Belmonte, Juan Carlos Izpisua

Protein & cell

2020 Volume 11, Issue 3, Page(s) 219–226

MeSH term(s)	Animals ; Cartilage, Articular/drug effects ; Cartilage, Articular/pathology ; Cell Culture Techniques ; Chondrocytes/drug effects ; Chondrocytes/pathology ; Glucuronidase/therapeutic use ; Humans ; Osteoarthritis, Knee/chemically induced ; Osteoarthritis, Knee/drug therapy ; Papain ; Rats ; Receptor, Transforming Growth Factor-beta Type II/therapeutic use ; Recombinant Proteins/therapeutic use
Chemical Substances	Recombinant Proteins ; Receptor, Transforming Growth Factor-beta Type II (EC 2.7.11.30) ; Glucuronidase (EC 3.2.1.31) ; klotho protein (EC 3.2.1.31) ; Papain (EC 3.4.22.2)
Language	English
Publishing date	2020-01-16
Publishing country	Germany
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	2543451-2
ISSN	1674-8018 ; 1674-800X
ISSN (online)	1674-8018
ISSN	1674-800X
DOI	10.1007/s13238-019-00685-7
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Article: Combined Hepatitis C Virus (HCV) Antigen-Antibody Detection Assay Does Not Improve Diagnosis for Seronegative Individuals with Occult HCV Infection

Quiroga, Juan A / Castillo, Inmaculada / Pardo, Margarita / Rodríguez-Iñigo, Elena / Carreño, Vicente

Journal of clinical microbiology JCM. 2006 Dec., v. 44, no. 12

2006

Abstract: A combined hepatitis C virus (HCV) antigen-antibody assay was evaluated for 115 seronegative individuals with occult HCV infection. The assay was reactive in one patient and negative to weakly reactive in three others (all four gave indeterminate results ...

Abstract	A combined hepatitis C virus (HCV) antigen-antibody assay was evaluated for 115 seronegative individuals with occult HCV infection. The assay was reactive in one patient and negative to weakly reactive in three others (all four gave indeterminate results by supplemental assay) but failed to detect HCV in the remaining patients. Despite increased sensitivity the combined assay does not improve serodiagnosis of occult HCV infection.
Language	English
Dates of publication	2006-12
Size	p. 4559-4560.
Publishing place	American Society for Microbiology
Document type	Article
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 1188: Show issues

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