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  1. Article ; Online: Mapping competitive pathways to terpenoid biosynthesis in Synechocystis sp. PCC 6803 using an antisense RNA synthetic tool.

    Rodrigues, João S / Bourgade, Barbara / Galle, Karen R / Lindberg, Pia

    Microbial cell factories

    2023  Volume 22, Issue 1, Page(s) 35

    Abstract: Background: Synechocystis sp. PCC 6803 utilizes pyruvate and glyceraldehyde 3-phosphate via the methylerythritol 4-phosphate (MEP) pathway for the biosynthesis of terpenoids. Considering the deep connection of the MEP pathway to the central carbon ... ...

    Abstract Background: Synechocystis sp. PCC 6803 utilizes pyruvate and glyceraldehyde 3-phosphate via the methylerythritol 4-phosphate (MEP) pathway for the biosynthesis of terpenoids. Considering the deep connection of the MEP pathway to the central carbon metabolism, and the low carbon partitioning towards terpenoid biosynthesis, significant changes in the metabolic network are required to increase cyanobacterial production of terpenoids.
    Results: We used the Hfq-MicC antisense RNA regulatory tool, under control of the nickel-inducible P
    Conclusions: We demonstrated that the Hfq-MicC synthetic tool can be used to evaluate the effects of gene knockdown on heterologous terpenoid production, despite the need for further optimization of the technique. Possible targets for future engineering of Synechocystis aiming at improved terpenoid microbial production were identified.
    MeSH term(s) Synechocystis/genetics ; Synechocystis/metabolism ; Chlorophyll A/metabolism ; Metabolic Engineering/methods ; Terpenes/metabolism ; Carbon/metabolism ; Amino Acids/metabolism
    Chemical Substances isoprene (0A62964IBU) ; Chlorophyll A (YF5Q9EJC8Y) ; Terpenes ; 2-C-methylerythritol 4-phosphate ; Carbon (7440-44-0) ; Amino Acids
    Language English
    Publishing date 2023-02-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091377-1
    ISSN 1475-2859 ; 1475-2859
    ISSN (online) 1475-2859
    ISSN 1475-2859
    DOI 10.1186/s12934-023-02040-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Metabolic engineering of

    Rodrigues, João S / Lindberg, Pia

    Metabolic engineering communications

    2020  Volume 12, Page(s) e00159

    Abstract: Terpenoids are a wide class of organic compounds with industrial relevance. The natural ability of cyanobacteria to produce terpenoids via the methylerythritol 4-phosphate (MEP) pathway makes these organisms appealing candidates for the generation of ... ...

    Abstract Terpenoids are a wide class of organic compounds with industrial relevance. The natural ability of cyanobacteria to produce terpenoids via the methylerythritol 4-phosphate (MEP) pathway makes these organisms appealing candidates for the generation of light-driven cell factories for green chemistry. Here we address the improvement of the production of (
    Language English
    Publishing date 2020-12-25
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2821894-2
    ISSN 2214-0301 ; 2214-0301
    ISSN (online) 2214-0301
    ISSN 2214-0301
    DOI 10.1016/j.mec.2020.e00159
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Characterizing isoprene production in cyanobacteria – insights into the effects of light, temperature, and isoprene on Synechocystis sp. PCC 6803

    Rodrigues, João S. / Kovács, László / Lukes, Martin / Hoeper, Rune / Steuer, Ralf / Červený, Jan / Lindberg, Pia / Zavřel, Tomáš

    Bioresource Technology. 2023 Apr. 19, p.129068-

    2023  , Page(s) 129068–

    Abstract: Engineering cyanobacteria for the production of isoprene and other terpenoids has gained increasing attention in the field of biotechnology. Several studies have addressed optimization of isoprene synthesis in cyanobacteria via enzyme and pathway ... ...

    Abstract Engineering cyanobacteria for the production of isoprene and other terpenoids has gained increasing attention in the field of biotechnology. Several studies have addressed optimization of isoprene synthesis in cyanobacteria via enzyme and pathway engineering. However, only little attention has been paid to the optimization of cultivation conditions. In this study, an isoprene-producing strain of Synechocystis sp. PCC 6803 and two control strains were grown under a variety of cultivation conditions. Isoprene production, as quantified by modified membrane inlet mass spectrometer (MIMS) and interpreted using Flux Balance Analysis (FBA), increased under violet light and at elevated temperature. Increase of thermotolerance in the isoprene producer was attributed to the physical presence of isoprene, similar to plants. The results demonstrate a beneficial effect of isoprene on cell survival at higher temperatures. This increased thermotolerance opens new possibilities for sustainable bio-production of isoprene and other products.
    Keywords Synechocystis ; biotechnology ; cell viability ; enzymes ; heat tolerance ; isoprene ; spectrometers ; temperature ; cyanobacteria ; cultivation conditions ; thermotolerance ; MIMS ; Flux Balance Analysis
    Language English
    Dates of publication 2023-0419
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2023.129068
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Characterizing isoprene production in cyanobacteria - Insights into the effects of light, temperature, and isoprene on Synechocystis sp. PCC 6803.

    Rodrigues, João S / Kovács, László / Lukeš, Martin / Höper, Rune / Steuer, Ralf / Červený, Jan / Lindberg, Pia / Zavřel, Tomáš

    Bioresource technology

    2023  Volume 380, Page(s) 129068

    Abstract: Engineering cyanobacteria for the production of isoprene and other terpenoids has gained increasing attention in the field of biotechnology. Several studies have addressed optimization of isoprene synthesis in cyanobacteria via enzyme and pathway ... ...

    Abstract Engineering cyanobacteria for the production of isoprene and other terpenoids has gained increasing attention in the field of biotechnology. Several studies have addressed optimization of isoprene synthesis in cyanobacteria via enzyme and pathway engineering. However, only little attention has been paid to the optimization of cultivation conditions. In this study, an isoprene-producing strain of Synechocystis sp. PCC 6803 and two control strains were grown under a variety of cultivation conditions. Isoprene production, as quantified by modified membrane inlet mass spectrometer (MIMS) and interpreted using Flux Balance Analysis (FBA), increased under violet light and at elevated temperature. Increase of thermotolerance in the isoprene producer was attributed to the physical presence of isoprene, similar to plants. The results demonstrate a beneficial effect of isoprene on cell survival at higher temperatures. This increased thermotolerance opens new possibilities for sustainable bio-production of isoprene and other products.
    MeSH term(s) Synechocystis/metabolism ; Temperature ; Hemiterpenes/metabolism ; Butadienes/metabolism
    Chemical Substances isoprene (0A62964IBU) ; Hemiterpenes ; Butadienes
    Language English
    Publishing date 2023-04-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2023.129068
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 3258: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

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  5. Article: Heterologous Production of Glycine Betaine Using

    Ferreira, Eunice A / Pacheco, Catarina C / Rodrigues, João S / Pinto, Filipe / Lamosa, Pedro / Fuente, David / Urchueguía, Javier / Tamagnini, Paula

    Frontiers in bioengineering and biotechnology

    2022  Volume 9, Page(s) 821075

    Abstract: Among compatible solutes, glycine betaine has various applications in the fields of nutrition, pharmaceuticals, and cosmetics. Currently, this compound can be extracted from sugar beet plants or obtained by chemical synthesis, resulting in low yields or ... ...

    Abstract Among compatible solutes, glycine betaine has various applications in the fields of nutrition, pharmaceuticals, and cosmetics. Currently, this compound can be extracted from sugar beet plants or obtained by chemical synthesis, resulting in low yields or high carbon footprint, respectively. Hence, in this work we aimed at exploring the production of glycine betaine using the unicellular cyanobacterium
    Language English
    Publishing date 2022-01-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2021.821075
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803.

    Dienst, Dennis / Wichmann, Julian / Mantovani, Oliver / Rodrigues, João S / Lindberg, Pia

    Scientific reports

    2020  Volume 10, Issue 1, Page(s) 5932

    Abstract: Cyanobacteria and microalgae are attractive photoautotrophic host systems for climate-friendly production of fuels and other value-added biochemicals. However, for economic applications further development and implementation of efficient and sustainable ... ...

    Abstract Cyanobacteria and microalgae are attractive photoautotrophic host systems for climate-friendly production of fuels and other value-added biochemicals. However, for economic applications further development and implementation of efficient and sustainable cultivation strategies are essential. Here, we present a comparative study on cyanobacterial sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803 using a commercial lab-scale High Density Cultivation (HDC) platform in the presence of dodecane as in-situ extractant. Operating in a two-step semi-batch mode over a period of eight days, volumetric yields of (E)-α-bisabolene were more than two orders of magnitude higher than previously reported for cyanobacteria, with final titers of 179.4 ± 20.7 mg * L
    MeSH term(s) Alkanes ; Cell Growth Processes ; Metabolic Engineering/methods ; Monocyclic Sesquiterpenes ; Photosynthesis ; Phototrophic Processes ; Sesquiterpenes/metabolism ; Synechocystis/physiology
    Chemical Substances Alkanes ; Monocyclic Sesquiterpenes ; Sesquiterpenes ; n-dodecane (11A386X1QH) ; bisabolol (24WE03BX2T)
    Language English
    Publishing date 2020-04-03
    Publishing country England
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-62681-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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