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Article: Mechanical Characterization at the Microscale of Mineralized Bone Callus after Bone Lengthening.

Roseren, Flavy / Roffino, Sandrine / Pithioux, Martine

2022 Volume 15, Issue 18

Abstract: Distraction osteogenesis (DO) involves several processes to form an organized distracted callus. While bone regeneration during DO has been widely described, no study has yet focused on the evolution profile of mechanical properties of mineralized ... ...

Abstract	Distraction osteogenesis (DO) involves several processes to form an organized distracted callus. While bone regeneration during DO has been widely described, no study has yet focused on the evolution profile of mechanical properties of mineralized tissues in the distracted callus. The aim of this study was therefore to measure the elastic modulus and hardness of calcified cartilage and trabecular and cortical bone within the distracted callus during the consolidation phase. We used a microindentation assay to measure the mechanical properties of periosteal and endosteal calluses; each was subdivided into two regions. Histological sections were used to localize the tissues. The results revealed that the mechanical properties of calcified cartilage did not evolve over time. However, trabecular bone showed temporal variation. For elastic modulus, in three out of four regions, a similar evolution profile was observed with an increase and decrease over time. Concerning hardness, this evolves differently depending on the location in the distracted callus. We also observed spatial changes in between regions. A first duality was apparent between regions close to the native cortices and the central area, while latter differences were seen between periosteal and endosteal calluses. Data showed a heterogeneity of mechanical properties in the distracted callus with a specific mineralization profile.
Language	English
Publishing date	2022-09-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma15186207
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Article: Characterization of the mechanical properties of the mouse Achilles tendon enthesis by microindentation. Effects of unloading and subsequent reloading.

Camy, Claire / Grünewald, Tilman / Lamy, Edouard / Roseren, Flavy / Caumes, Mathieu / Fovet, Théo / Brioche, Thomas / Genovesio, Cecile / Chopard, Angèle / Pithioux, Martine / Roffino, Sandrine

Bone reports

2024 Volume 20, Page(s) 101734

Abstract: The fibrocartilaginous tendon enthesis, i.e. the site where a tendon is attached to bone through a fibrocartilaginous tissue, is considered as a functionally graded interface. However, at local scale, a very limited number of studies have characterized ... ...

Abstract	The fibrocartilaginous tendon enthesis, i.e. the site where a tendon is attached to bone through a fibrocartilaginous tissue, is considered as a functionally graded interface. However, at local scale, a very limited number of studies have characterized micromechanical properties of this transitional tissue. The first goal of this work was to characterize the micromechanical properties of the mineralized part of the healthy Achilles tendon enthesis (ATE) through microindentation testing and to assess the degree of mineralization and of carbonation of mineral crystals by Raman spectroscopy. Since little is known about enthesis biological plasticity, our second objective was to examine the effects of unloading and reloading, using a mouse hindlimb-unloading model, on both the micromechanical properties and the mineral phase of the ATE. Elastic modulus, hardness, degree of mineralization, and degree of carbonation were assessed after 14 days of hindlimb suspension and again after a subsequent 6 days of reloading. The elastic modulus gradually increased along the mineralized part of the ATE from the tidemark to the subchondral bone, with the same trend being found for hardness. Whereas the degree of carbonation did not differ according to zone of measurement, the degree of mineralization increased by >70 % from tidemark to subchondral bone. Thus, the gradient in micromechanical properties is in part explained by a mineralization gradient. A 14-day unloading period did not appear to affect the gradient of micromechanical properties of the ATE, nor the degree of mineralization or carbonation. However, contrary to a short period of unloading, early return to normal mechanical load reduced the micromechanical properties gradient, regardless of carbonate-to-phosphate ratios, likely due to the more homogeneous degree of mineralization. These findings provide valuable data not only for tissue bioengineering, but also for musculoskeletal clinical studies and microgravity studies focusing on long-term space travel by astronauts.
Language	English
Publishing date	2024-01-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	2821774-3
ISSN	2352-1872
ISSN	2352-1872
DOI	10.1016/j.bonr.2024.101734
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Article ; Online: Probing the Cellular Size Distribution in Cell Samples Undergoing Cell Death.

Franceschini, Emilie / Balasse, Laure / Roffino, Sandrine / Guillet, Benjamin

Ultrasound in medicine & biology

2019 Volume 45, Issue 7, Page(s) 1787–1798

Abstract: A polydisperse scattering model adapted for concentrated medium, namely the polydisperse structure factor model, was examined to explain the backscatter coefficients (BSCs) measured from packed cell samples undergoing cell death. Cell samples were ... ...

Abstract	A polydisperse scattering model adapted for concentrated medium, namely the polydisperse structure factor model, was examined to explain the backscatter coefficients (BSCs) measured from packed cell samples undergoing cell death. Cell samples were scanned using high-frequency ultrasound in the 10-42 MHz bandwidth. A parameter estimation procedure was proposed to estimate the volume fraction and the relative impedance contrast that could explain the changes in BSC pattern by considering the actual change in cellular size distribution. Quantitative ultrasound parameters were estimated and related to the percentage of dead cells determined by flow cytometry. The standard deviation of scatterer size distribution extracted from the polydisperse structure factor model and the spectral intercept were found to be strongly correlated to the percentage of dead cells (r
MeSH term(s)	Adenocarcinoma/pathology ; Apoptosis/physiology ; Cell Culture Techniques ; Cell Death/physiology ; Colonic Neoplasms/pathology ; Flow Cytometry/methods ; Humans ; In Vitro Techniques ; Phantoms, Imaging ; Ultrasonography/methods
Language	English
Publishing date	2019-04-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	186150-5
ISSN	1879-291X ; 0301-5629
ISSN (online)	1879-291X
ISSN	0301-5629
DOI	10.1016/j.ultrasmedbio.2019.01.006
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Article ; Online: Mortality, cardiac and cerebral damages reduction by IL-1 inhibition in a murine model of TTP.

Muller, Romain / Cauchois, Raphael / Lagarde, Marie / Roffino, Sandrine / Genovesio, Cecile / Fernandez, Samantha / Hache, Guillaume / Guillet, Benjamin / Kara, Yeter / Marlinge, Marion / Lenting, Peter J / Poullin, Pascale / Dignat-George, Françoise / Tellier, Edwige / Kaplanski, Gilles

Blood

2024

Abstract: Thrombotic thrombocytopenic purpura (TTP), a rare but fatal disease if untreated, is due to alteration in Von Willebrand factor cleavage resulting in capillary microthrombi formation and ischemic organ damage. Interleukin-1 (IL-1), has been shown to ... ...

Abstract	Thrombotic thrombocytopenic purpura (TTP), a rare but fatal disease if untreated, is due to alteration in Von Willebrand factor cleavage resulting in capillary microthrombi formation and ischemic organ damage. Interleukin-1 (IL-1), has been shown to drive sterile inflammation following ischemia and could play an essential contribution to post-ischemic organ damage in TTP. Our objectives were to evaluate IL-1 involvement during TTP and to test the efficacy of the recombinant IL-1 receptor antagonist, anakinra, in a murine TTP model. We retrospectively measured plasmatic IL-1 concentrations in TTP patients and controls. TTP patients exhibited elevated plasma IL-1α and β concentrations, which correlated with disease course and survival. In a TTP mouse model, we administered anakinra (IL-1 inhibitor) or placebo for 5 days and evaluated the efficacy of this treatment. Anakinra significantly reduced mortality of mice (P<0.001). Anakinra significantly decreased TTP-induced cardiac damages as assessed by blood troponin concentrations, evaluation of left ventricular function by echocardiography, [18F]FDG PET of myocardial glucose metabolism, and cardiac histology. Anakinra also significantly reduced brain TTP-induced damages, evaluated through blood PS100b concentrations, nuclear imaging and histology. We finally showed that IL-1α and β trigger endothelial degranulation in vitro, leading to the release of Von Willebrand factor. In conclusion, Anakinra significantly reduced TTP mortality in a pre-clinical model of the disease by inhibiting both endothelial degranulation and post-ischemic inflammation, supporting further evaluations in humans.
Language	English
Publishing date	2024-04-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	80069-7
ISSN	1528-0020 ; 0006-4971
ISSN (online)	1528-0020
ISSN	0006-4971
DOI	10.1182/blood.2023021974
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Article ; Online: Systemic Administration of G-CSF Accelerates Bone Regeneration and Modulates Mobilization of Progenitor Cells in a Rat Model of Distraction Osteogenesis.

Roseren, Flavy / Pithioux, Martine / Robert, Stéphane / Balasse, Laure / Guillet, Benjamin / Lamy, Edouard / Roffino, Sandrine

International journal of molecular sciences

2021 Volume 22, Issue 7

Abstract: Granulocyte colony-stimulating factor (G-CSF) was shown to promote bone regeneration and mobilization of vascular and osteogenic progenitor cells. In this study, we investigated the effects of a systemic low dose of G-CSF on both bone consolidation and ... ...

Abstract	Granulocyte colony-stimulating factor (G-CSF) was shown to promote bone regeneration and mobilization of vascular and osteogenic progenitor cells. In this study, we investigated the effects of a systemic low dose of G-CSF on both bone consolidation and mobilization of hematopoietic stem/progenitor cells (HSPCs), endothelial progenitor cells (EPCs) and mesenchymal stromal cells (MSCs) in a rat model of distraction osteogenesis (DO). Neovascularization and mineralization were longitudinally monitored using positron emission tomography and planar scintigraphy. Histological analysis was performed and the number of circulating HSPCs, EPCs and MSCs was studied by flow cytometry. Contrary to control group, in the early phase of consolidation, a bony bridge with lower osteoclast activity and a trend of an increase in osteoblast activity were observed in the distracted callus in the G-CSF group, whereas, at the late phase of consolidation, a significantly lower neovascularization was observed. While no difference was observed in the number of circulating EPCs between control and G-CSF groups, the number of MSCs was significantly lower at the end of the latency phase and that of HSPCs was significantly higher 4 days after the bone lengthening. Our results indicate that G-CSF accelerates bone regeneration and modulates mobilization of progenitor cells during DO.
MeSH term(s)	Animals ; Bone Regeneration/drug effects ; Disease Models, Animal ; Durapatite/chemistry ; Flow Cytometry ; Granulocyte Colony-Stimulating Factor/administration & dosage ; Hematopoietic Stem Cell Mobilization ; Kinetics ; Male ; Mesenchymal Stem Cells/cytology ; Neovascularization, Physiologic/drug effects ; Osteoblasts/metabolism ; Osteoclasts/drug effects ; Osteogenesis, Distraction ; Positron-Emission Tomography ; Rats ; Rats, Sprague-Dawley ; Single Photon Emission Computed Tomography Computed Tomography ; Stem Cells/cytology ; Stem Cells/metabolism
Chemical Substances	Granulocyte Colony-Stimulating Factor (143011-72-7) ; Durapatite (91D9GV0Z28)
Language	English
Publishing date	2021-03-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22073505
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Article ; Online: Effects of hindlimb unloading and subsequent reloading on the structure and mechanical properties of Achilles tendon-to-bone attachment.

Camy, Claire / Brioche, Thomas / Senni, Karim / Bertaud, Alexandrine / Genovesio, Cécile / Lamy, Edouard / Fovet, Théo / Chopard, Angèle / Pithioux, Martine / Roffino, Sandrine

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

2022 Volume 36, Issue 10, Page(s) e22548

Abstract: While muscle and bone adaptations to deconditioning have been widely described, few studies have focused on the tendon enthesis. Our study examined the effects of mechanical loading on the structure and mechanical properties of the Achilles tendon ... ...

Abstract	While muscle and bone adaptations to deconditioning have been widely described, few studies have focused on the tendon enthesis. Our study examined the effects of mechanical loading on the structure and mechanical properties of the Achilles tendon enthesis. We assessed the fibrocartilage surface area, the organization of collagen, the expression of collagen II, the presence of osteoclasts, and the tensile properties of the mouse enthesis both after 14 days of hindlimb suspension (HU) and after a subsequent 6 days of reloading. Although soleus atrophy was severe after HU, calcified fibrocartilage (CFc) was a little affected. In contrast, we observed a decrease in non-calcified fibrocartilage (UFc) surface area, collagen fiber disorganization, modification of morphological characteristics of the fibrocartilage cells, and altered collagen II distribution. Compared to the control group, restoring normal loads increased both UFc surface area and expression of collagen II, and led to a crimp pattern in collagen. Reloading induced an increase in CFc surface area, probably due to the mineralization front advancing toward the tendon. Functionally, unloading resulted in decreased enthesis stiffness and a shift in site of failure from the osteochondral interface to the bone, whereas 6 days of reloading restored the original elastic properties and site of failure. In the context of spaceflight, our results suggest that care must be taken when performing countermeasure exercises both during missions and during the return to Earth.
MeSH term(s)	Achilles Tendon/metabolism ; Animals ; Bone and Bones ; Collagen/metabolism ; Hindlimb Suspension ; Mice ; Muscle, Skeletal/metabolism
Chemical Substances	Collagen (9007-34-5)
Language	English
Publishing date	2022-09-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639186-2
ISSN	1530-6860 ; 0892-6638
ISSN (online)	1530-6860
ISSN	0892-6638
DOI	10.1096/fj.202200713R
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Zs.A 2266: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Oral manifestations associated with inherited hyperhomocysteinemia: A first case description.

Husseini, Bachar / Nehme, Edgard / Senni, Karim / Ghorra, Claude Sader / Younes, Khalil / Roffino, Sandrine / Ghorra, Pierre / Changotade, Sylvie / Younes, Ronald

Oral surgery, oral medicine, oral pathology and oral radiology

2021 Volume 133, Issue 5, Page(s) e105–e112

Abstract: Hyperhomocysteinemia is a rare disease caused by nutritional deficiencies or genetic impairment of cysteine metabolism. To date, no oral manifestations of hyperhomocysteinemia have been described in humans. Therefore, to our knowledge, the present case ... ...

Abstract	Hyperhomocysteinemia is a rare disease caused by nutritional deficiencies or genetic impairment of cysteine metabolism. To date, no oral manifestations of hyperhomocysteinemia have been described in humans. Therefore, to our knowledge, the present case report is the first description of a hyperhomocysteinemic patient showing oral tissue alterations leading to both early tooth loss and failed implant osseointegration. The patient presented with a methylenetetrahydrofolate reductase gene mutation (677T polymorphism) leading to mild hyperhomocysteinemia. The radiologic analysis showed hyperdense lesions scattered in the maxillae. The histologic observations indicated alterations in both collagen and elastic networks in the gingiva and dermis. Interestingly, the presence of ectopic mineralized inclusions was noted in both periodontal ligament and gingiva. Strong osteoclastic activity was associated with abnormal calcification of trabecular spaces. Uneven oral tissue remodeling due to high tissue levels of homocysteine could explain the pathologic manifestations observed in this case.
MeSH term(s)	Humans ; Hyperhomocysteinemia/complications ; Hyperhomocysteinemia/genetics ; Methylenetetrahydrofolate Reductase (NADPH2)/genetics ; Polymorphism, Genetic
Chemical Substances	Methylenetetrahydrofolate Reductase (NADPH2) (EC 1.5.1.20)
Language	English
Publishing date	2021-09-20
Publishing country	United States
Document type	Case Reports
ZDB-ID	2650843-6
ISSN	2212-4411 ; 2212-4403
ISSN (online)	2212-4411
ISSN	2212-4403
DOI	10.1016/j.oooo.2021.09.007
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Article ; Online: Early-stage knee OA induced by MIA and MMT compared in the murine model via histological and topographical approaches.

Aüllo-Rasser, Gaetan / Dousset, Erick / Roffino, Sandrine / Zahouani, Hassan / Lecurieux-Clerville, Roger / Argenson, Jean-Noël / Chabrand, Patrick

Scientific reports

2020 Volume 10, Issue 1, Page(s) 15430

Abstract: Osteoarthritis (OA) is a common degenerative disease whose early management includes promising mechanical treatments. New treatments are initially validated using an animal model in which OA is induced. The MMT (mechanical induction) and MIA (chemical ... ...

Abstract	Osteoarthritis (OA) is a common degenerative disease whose early management includes promising mechanical treatments. New treatments are initially validated using an animal model in which OA is induced. The MMT (mechanical induction) and MIA (chemical induction) models of OA induction are widespread, but their use to generate early OA is poorly documented. We analyzed and compared early-stage knee OA-induction via these two methods in 16 rats divided into two groups. After 4 weeks of induction, the knees were sampled and studied using both histology (Toluidine Blue and Sirius Red) and surface topology, an innovative technique for characterizing osteoarthritic cartilage. The Mankin-modified score confirms that the two OA-induction models evolved at the same speed. At this early stage, the two models can be differentiated morphologically, although no significant differences were revealed by either cellularity or birefringence analysis. However, the topological analysis generated two forms of quantitative data, the deformation ratio and the cohesion index, that differentiated between the two groups. Thus, the early-stage OA induced by these two models is revealed to differ. The patterns of cartilage damage induced point to MMT as the better choice to assess mechanical approaches to clinical OA treatment.
MeSH term(s)	Animals ; Arthritis, Experimental/pathology ; Cartilage, Articular/pathology ; Disease Models, Animal ; Male ; Osteoarthritis, Knee/pathology ; Rats ; Rats, Sprague-Dawley
Language	English
Publishing date	2020-09-22
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-72350-7
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Article ; Online: Total Knee Arthroplasty with a Ti6Al4V/PEEK Prosthesis on an Osteoarthritis Rat Model: Behavioral and Neurophysiological Analysis.

Lecocq, Mathieu / Linares, Jean-Marc / Chaves-Jacob, Julien / Coyle, Thelma / Roffino, Sandrine / Eyraud, Marielle / Gigmes, Didier / Decherchi, Patrick / Dousset, Erick

Scientific reports

2020 Volume 10, Issue 1, Page(s) 5277

Abstract: Arthroplasty is a surgical procedure to restore the function of the joint of patient suffering from knee osteoarthritis. However, postoperative functional deficits are reported even after a rehabilitation program. In order to determine the origin of ... ...

Abstract	Arthroplasty is a surgical procedure to restore the function of the joint of patient suffering from knee osteoarthritis. However, postoperative functional deficits are reported even after a rehabilitation program. In order to determine the origin of functional deficits of patient suffering from knee osteoarthritis and total knee arthroplasty, we developed a rodent model including a chemically-induced-osteoarthritis and designed a knee prosthesis (Ti6Al4V/PEEK) biomechanically and anatomically adapted to rat knee joint. Dynamic Weight-Bearing, gait kinematics, H-reflex from vastus medialis muscle and activities from metabosensitive III and IV afferent fibers in femoral nerve were assessed at 1 and 3 months post-surgery. Results indicate that knee osteoarthritis altered considerably the responses of afferent fibers to their known activators (i.e., lactic acid and potassium chloride) and consequently their ability to modulate the spinal sensorimotor loop, although, paradoxically, motor deficits seemed relatively light. On the contrary, results indicate that, after the total knee arthroplasty, the afferent responses and the sensorimotor function were slightly altered but that motor deficits were more severe. We conclude that neural changes attested by the recovery of the metabosensitive afferent activity and the sensorimotor loop were induced when a total knee replacement was performed and that these changes may disrupt or delay the locomotor recovery.
MeSH term(s)	Afferent Pathways/physiology ; Animals ; Arthroplasty, Replacement, Knee/adverse effects ; Arthroplasty, Replacement, Knee/instrumentation ; Arthroplasty, Replacement, Knee/methods ; Disease Models, Animal ; Femoral Nerve/injuries ; Femoral Nerve/physiopathology ; Gait ; Gait Disorders, Neurologic/etiology ; Gait Disorders, Neurologic/physiopathology ; H-Reflex ; Iodoacetic Acid/toxicity ; Ketones ; Knee Prosthesis ; Male ; Muscle, Skeletal/physiopathology ; Osteoarthritis, Knee/chemically induced ; Osteoarthritis, Knee/surgery ; Peripheral Nerve Injuries/etiology ; Peripheral Nerve Injuries/physiopathology ; Polyethylene Glycols ; Postoperative Complications/etiology ; Random Allocation ; Rats ; Recovery of Function ; Titanium ; Weight-Bearing
Chemical Substances	Ketones ; titanium alloy (TiAl6V4) (12743-70-3) ; polyetheretherketone (31694-16-3) ; Polyethylene Glycols (3WJQ0SDW1A) ; Titanium (D1JT611TNE) ; Iodoacetic Acid (WF5188V710)
Language	English
Publishing date	2020-03-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-62146-0
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Article ; Online: Granulocyte-colony stimulating factor enhances bone fracture healing.

Moukoko, Didier / Pourquier, Didier / Genovesio, Cécile / Thezenas, Simon / Chabrand, Patrick / Roffino, Sandrine / Pithioux, Martine

Clinical biomechanics (Bristol, Avon)

2018 Volume 58, Page(s) 62–68

Abstract: Background: Circulating mesenchymal stem cells contribute to bone repair. Their incorporation in fracture callus is correlated to their bioavailability. In addition, Granulocyte-colony stimulating factor induces the release of vascular and mesenchymal ... ...

Abstract	Background: Circulating mesenchymal stem cells contribute to bone repair. Their incorporation in fracture callus is correlated to their bioavailability. In addition, Granulocyte-colony stimulating factor induces the release of vascular and mesenchymal progenitors. We hypothesized that this glycoprotein stimulates fracture healing, and analyzed the effects of its administration at low doses on bone healing. Methods: 27 adult male Sprague-Dawley rats underwent mid-femur osteotomy stabilized by centromedullar pinning. In a post (pre) operative group, rats were subcutaneously injected with 5 μg/kg per day of Granulocyte-colony stimulating factor for 5 days after (before) surgery. In a control group, rats were injected with saline solution for 5 days immediately after surgery. A radiographic consolidation score was calculated. At day 35, femurs were studied histologically and underwent biomechanical tests. Findings: 5 weeks after surgery, mean radiographic scores were significantly higher in the Preop group 7.75 (SD 0.42) and in the Postop group 7.67 (SD 0.52) than in the control group 6.75 (SD 0.69). Biomechanical tests showed femur stiffness to be more than three times higher in both the Preop 109.24 N/mm (SD 51.86) and Postop groups 100.05 N/mm (SD 60.24) than in control 32.01 N/mm (SD 15.78). Mean maximal failure force was twice as high in the Preop group 68.66 N (SD 27.78) as in the control group 34.21 N (SD 11.79). Histological results indicated a later consolidation process in control than in treated groups. Interpretation: Granulocyte-colony stimulating factor injections strongly stimulated early femur fracture healing, indicating its potential utility in human clinical situations such as programmed osteotomy and fracture.
MeSH term(s)	Animals ; Bone Nails ; Bony Callus/physiology ; Femoral Fractures/physiopathology ; Femoral Fractures/surgery ; Fracture Fixation, Intramedullary ; Fracture Healing/drug effects ; Fracture Healing/physiology ; Granulocyte Colony-Stimulating Factor/administration & dosage ; Humans ; Male ; Osteotomy ; Rats ; Rats, Sprague-Dawley
Chemical Substances	Granulocyte Colony-Stimulating Factor (143011-72-7)
Language	English
Publishing date	2018-07-20
Publishing country	England
Document type	Journal Article
ZDB-ID	632747-3
ISSN	1879-1271 ; 0268-0033
ISSN (online)	1879-1271
ISSN	0268-0033
DOI	10.1016/j.clinbiomech.2018.07.010
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