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  1. AU="Roger Le Grand"
  2. AU="Tesfalidet, Solomon"
  3. AU="Geladari, Eleni"
  4. AU="Pallabi Mustafi"
  5. AU=Mountfort Katrina
  6. AU="Horne, Patrick"
  7. AU="Mhurchu, Cliona Ni"
  8. AU="Yatoo, Ali Mohd"
  9. AU="Zhang, Zhiru"
  10. AU="Hadie Adams"
  11. AU="Gaskin, Thomas R"
  12. AU="Guo, Chong"
  13. AU="Guocan Wang"
  14. AU="Catherine Crenn-Hebert"
  15. AU="Alistar, Cristina F"
  16. AU="Makhani, Sarah S"
  17. AU="Tayyeb Pourfallah"
  18. AU="Mauad, Thais"

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Treffer 1 - 10 von insgesamt 65

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  1. Artikel ; Online: Special Issue “Immune Ontogeny and Vaccination in Early Life

    Nabila Seddiki / Roger Le Grand

    Vaccines, Vol 9, Iss 1014, p

    How the Non-Human Primate Model Can Help Expand the Current Knowledge in Pediatric Immunology and Infectious Diseases Research”

    2021  Band 1014

    Abstract: The development of the immune system requires a number of changes that occur during the first months of life [.] ...

    Abstract The development of the immune system requires a number of changes that occur during the first months of life [.]
    Schlagwörter n/a ; Medicine ; R
    Sprache Englisch
    Erscheinungsdatum 2021-09-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Impact of a PMMA tube on performances of a Vereos PET/CT system adapted for BSL-3 environment according to the NEMA NU2-2012 standard

    Nidhal Kahlaoui / Thibaut Naninck / Roger Le Grand / Catherine Chapon

    EJNMMI Physics, Vol 9, Iss 1, Pp 1-

    2022  Band 10

    Abstract: Abstract Introduction A Vereos PET/CT device was adapted to be compatible with the experimentation in large animals within BSL-3 environment. The aim of this study was to investigate the impact of this modification on the performance according to NEMA ... ...

    Abstract Abstract Introduction A Vereos PET/CT device was adapted to be compatible with the experimentation in large animals within BSL-3 environment. The aim of this study was to investigate the impact of this modification on the performance according to NEMA NU2-2012 standard. Methods Spatial resolution, sensitivity, count rate performance, accuracies of corrections and image quality were assessed using the NEMA NU2-2012 standards before and after installation of a transparent poly-methyl methacrylate tube of 8 mm thickness, 680 mm diameter and 2800 mm long inside the tunnel of the system. In addition, CT performance tests were performed according to manufacturer standard procedure. Results Although the presence of the tube led to a slight decrease in sensitivity, performance measurements were in accordance with manufacturer preconisation ranges and comparable to previous performance published data. Conclusion Modifications of Vereos PET/CT system allowing its use in BSL-3 conditions did not affect significantly its performance according to NEMA NU2-2012 standard. Key points Question. Does a BSL-3 compatible modification alter Philips Vereos PET/CT performances according to NEMA NU2-2012 standards? Pertinent findings. Our Vereos PET/CT system was modified by a wall separating BSL-1 and BSL-3 sides and an 8 mm thickness PMMA tube inserted into the bore of the camera in order to extend the BSL-3 containment along the bed movement. The performances of our modified system according to NEMA NU2-2012 standards were not significantly impacted by the modifications and were in accordance with the values prescribed by the manufacturer. Implications for patients care. Our clinical PET/CT device was modified for human infectious diseases studies in Non-Human Primates. This unusual set up may then provide truly transposable data from preclinical studies into clinical application in infected patients.
    Schlagwörter PET/CT ; NEMA NU2-2012 standard ; BSL-3 conditions ; Infectious diseases ; Medical physics. Medical radiology. Nuclear medicine ; R895-920
    Sprache Englisch
    Erscheinungsdatum 2022-03-01T00:00:00Z
    Verlag SpringerOpen
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Combined treatment of molnupiravir and favipiravir against SARS-CoV-2 infection

    Philippine Eloy / Roger Le Grand / Denis Malvy / Jérémie Guedj

    EBioMedicine, Vol 74, Iss , Pp 103663- (2021)

    One + zero equals two?

    2021  

    Schlagwörter Medicine ; R ; Medicine (General) ; R5-920
    Sprache Englisch
    Erscheinungsdatum 2021-12-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
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  4. Artikel ; Online: Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells

    Keltouma Benmeziane / Benoit Delache / Sébastien Langlois / Gabriella Scarlatti / Roger Le Grand / Mariangela Cavarelli

    STAR Protocols, Vol 3, Iss 4, Pp 101815- (2022)

    2022  

    Abstract: Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina ... ...

    Abstract Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications.For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
    Schlagwörter Cell isolation ; Flow Cytometry/Mass Cytometry ; Immunology ; Science (General) ; Q1-390
    Thema/Rubrik (Code) 004
    Sprache Englisch
    Erscheinungsdatum 2022-12-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: Early antiretroviral therapy favors post-treatment SIV control associated with the expansion of enhanced memory CD8+ T-cells

    Caroline Passaes / Delphine Desjardins / Anaïs Chapel / Valérie Monceaux / Julien Lemaitre / Adeline Mélard / Federico Perdomo-Celis / Cyril Planchais / Maël Gourvès / Nastasia Dimant / Annie David / Nathalie Dereuddre-Bosquet / Aurélie Barrail-Tran / Hélène Gouget / Céline Guillaume / Francis Relouzat / Olivier Lambotte / Jérémie Guedj / Michaela Müller-Trutwin /
    Hugo Mouquet / Christine Rouzioux / Véronique Avettand-Fenoël / Roger Le Grand / Asier Sáez-Cirión

    Nature Communications, Vol 15, Iss 1, Pp 1-

    2024  Band 19

    Abstract: Abstract HIV remission can be achieved in some people, called post-treatment HIV controllers, after antiretroviral treatment discontinuation. Treatment initiation close to the time of infection was suggested to favor post-treatment control, but the ... ...

    Abstract Abstract HIV remission can be achieved in some people, called post-treatment HIV controllers, after antiretroviral treatment discontinuation. Treatment initiation close to the time of infection was suggested to favor post-treatment control, but the circumstances and mechanisms leading to this outcome remain unclear. Here we evaluate the impact of early (week 4) vs. late (week 24 post-infection) treatment initiation in SIVmac251-infected male cynomolgus macaques receiving 2 years of therapy before analytical treatment interruption. We show that early treatment strongly promotes post-treatment control, which is not related to a lower frequency of infected cells at treatment interruption. Rather, early treatment favors the development of long-term memory CD8+ T cells with enhanced proliferative and SIV suppressive capacity that are able to mediate a robust secondary-like response upon viral rebound. Our model allows us to formally demonstrate a link between treatment initiation during primary infection and the promotion of post-treatment control and provides results that may guide the development of new immunotherapies for HIV remission.
    Schlagwörter Science ; Q
    Thema/Rubrik (Code) 616
    Sprache Englisch
    Erscheinungsdatum 2024-01-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Sotrovimab retains activity against SARS-CoV-2 omicron variant BQ.1.1 in a non-human primate model

    Cécile Hérate / Romain Marlin / Franck Touret / Nathalie Dereuddre-Bosquet / Flora Donati / Francis Relouzat / Laura Junges / Mathilde Galhaut / Océane Dehan / Quentin Sconosciuti / Antoine Nougairède / Xavier de Lamballerie / Sylvie van der Werf / Roger Le Grand

    Heliyon, Vol 9, Iss 6, Pp e16664- (2023)

    2023  

    Abstract: The SARS-CoV2 Omicron variants have acquired new Spike mutations leading to escape from the most of the currently available monoclonal antibody treatments reducing the options for patients suffering from severe Covid-19. Recently, both in vitro and in ... ...

    Abstract The SARS-CoV2 Omicron variants have acquired new Spike mutations leading to escape from the most of the currently available monoclonal antibody treatments reducing the options for patients suffering from severe Covid-19. Recently, both in vitro and in vivo data have suggested that Sotrovimab could retain partial activity against recent omicron sub-lineage such as BA.5 variants, including BQ.1.1. Here we report full efficacy of Sotrovimab against BQ.1.1 viral replication as measure by RT-qPCR in a non-human primate challengemodel.
    Schlagwörter SARS-CoV2 ; Sotrovimab ; Non human primate ; BQ.1.1 ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Sprache Englisch
    Erscheinungsdatum 2023-06-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel ; Online: Effectiveness of CHIKV vaccine VLA1553 demonstrated by passive transfer of human sera

    Pierre Roques / Andrea Fritzer / Nathalie Dereuddre-Bosquet / Nina Wressnigg / Romana Hochreiter / Laetitia Bossevot / Quentin Pascal / Fabienne Guehenneux / Annegret Bitzer / Irena Corbic Ramljak / Roger Le Grand / Urban Lundberg / Andreas Meinke

    JCI Insight, Vol 7, Iss

    2022  Band 14

    Abstract: Chikungunya virus (CHIKV) is a reemerging mosquito-borne alphavirus responsible for numerous outbreaks. Chikungunya can cause debilitating acute and chronic disease. Thus, the development of a safe and effective CHIKV vaccine is an urgent global health ... ...

    Abstract Chikungunya virus (CHIKV) is a reemerging mosquito-borne alphavirus responsible for numerous outbreaks. Chikungunya can cause debilitating acute and chronic disease. Thus, the development of a safe and effective CHIKV vaccine is an urgent global health priority. This study evaluated the effectiveness of the live-attenuated CHIKV vaccine VLA1553 against WT CHIKV infection by using passive transfer of sera from vaccinated volunteers to nonhuman primates (NHP) subsequently exposed to WT CHIKV and established a serological surrogate of protection. We demonstrated that human VLA1553 sera transferred to NHPs conferred complete protection from CHIKV viremia and fever after challenge with homologous WT CHIKV. In addition, serum transfer protected animals from other CHIKV-associated clinical symptoms and from CHIKV persistence in tissue. Based on this passive transfer study, a 50% micro–plaque reduction neutralization test titer of ≥ 150 was determined as a surrogate of protection, which was supported by analysis of samples from a seroepidemiological study. In conclusion, considering the unfeasibility of an efficacy trial due to the unpredictability and explosive, rapidly moving nature of chikungunya outbreaks, the definition of a surrogate of protection for VLA1553 is an important step toward vaccine licensure to reduce the medical burden caused by chikungunya.
    Schlagwörter Infectious disease ; Vaccines ; Medicine ; R
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2022-07-01T00:00:00Z
    Verlag American Society for Clinical investigation
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: Identification of CX3CR1+ mononuclear phagocyte subsets involved in HIV-1 and SIV colorectal transmission

    Mariangela Cavarelli / Chiara Foglieni / Naima Hantour / Tilo Schorn / Antonello Ferrazzano / Stefania Dispinseri / Delphine Desjardins / Ugo Elmore / Nathalie Dereuddre-Bosquet / Gabriella Scarlatti / Roger Le Grand

    iScience, Vol 25, Iss 6, Pp 104346- (2022)

    2022  

    Abstract: Summary: The difficulty to unambiguously identify the various subsets of mononuclear phagocytes (MNPs) of the intestinal lamina propria has hindered our understanding of the initial events occurring after mucosal exposure to HIV-1.Here, we compared the ... ...

    Abstract Summary: The difficulty to unambiguously identify the various subsets of mononuclear phagocytes (MNPs) of the intestinal lamina propria has hindered our understanding of the initial events occurring after mucosal exposure to HIV-1.Here, we compared the composition and function of MNP subsets at steady-state and following ex vivo and in vivo viral exposure in human and macaque colorectal tissues.Combined evaluation of CD11c, CD64, CD103, and CX3CR1 expression allowed to differentiate lamina propria MNPs subsets common to both species. Among them, CD11c+ CX3CR1+ cells expressing CCR5 migrated inside the epithelium following ex vivo and in vivo exposure of colonic tissue to HIV-1 or SIV. In addition, the predominant population of CX3CR1high macrophages present at steady-state partially shifted to CX3CR1low macrophages as early as three days following in vivo SIV rectal challenge of macaques.Our analysis identifies CX3CR1+ MNPs as novel players in the early events of HIV-1 and SIV colorectal transmission.
    Schlagwörter Physiology ; Molecular biology ; Immunology ; Science ; Q
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2022-06-01T00:00:00Z
    Verlag Elsevier
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  9. Artikel ; Online: Detection of SARS-CoV-2 in subcutaneous fat but not visceral fat, and the disruption of fat lymphocyte homeostasis in both fat tissues in the macaque

    Anaëlle Olivo / Romain Marlin / Thierry Lazure / Pauline Maisonnasse / Laetitia Bossevot / Christelliah Mouanga / Julien Lemaitre / Guillaume Pourcher / Stéphane Benoist / Roger Le Grand / Olivier Lambotte / Nathalie Dereuddre-Bosquet / Christine Bourgeois

    Communications Biology, Vol 5, Iss 1, Pp 1-

    2022  Band 10

    Abstract: Subcutaneous fat tissue expresses higher angiotensin-converting-enzyme 2 mRNA than visceral fat tissue and is selectively infected by SARS-Cov-2, while both fat tissues show drastic reduction in CD69 expression in T cells. ...

    Abstract Subcutaneous fat tissue expresses higher angiotensin-converting-enzyme 2 mRNA than visceral fat tissue and is selectively infected by SARS-Cov-2, while both fat tissues show drastic reduction in CD69 expression in T cells.
    Schlagwörter Biology (General) ; QH301-705.5
    Sprache Englisch
    Erscheinungsdatum 2022-06-01T00:00:00Z
    Verlag Nature Portfolio
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  10. Artikel ; Online: Isotopic Radiolabeling of the Antiretroviral Drug [ 18 F]Dolutegravir for Pharmacokinetic PET Imaging

    Marion Tisseraud / Sébastien Goutal / Thomas Bonasera / Maud Goislard / Delphine Desjardins / Roger Le Grand / Chris M. Parry / Nicolas Tournier / Bertrand Kuhnast / Fabien Caillé

    Pharmaceuticals, Vol 15, Iss 587, p

    2022  Band 587

    Abstract: Deciphering the drug/virus/host interactions at infected cell reservoirs is a key leading to HIV-1 remission for which positron emission tomography (PET) imaging using radiolabeled antiretroviral (ARV) drugs is a powerful asset. Dolutegravir (DTG) is one ...

    Abstract Deciphering the drug/virus/host interactions at infected cell reservoirs is a key leading to HIV-1 remission for which positron emission tomography (PET) imaging using radiolabeled antiretroviral (ARV) drugs is a powerful asset. Dolutegravir (DTG) is one of the preferred therapeutic options to treat HIV and can be isotopically labeled with fluorine-18. [ 18 F]DTG was synthesized via a three-step approach of radiofluorination/nitrile reduction/peptide coupling with optimization for each step. Radiofluorination was performed on 2-fluoro-4-nitrobenzonitrile in 90% conversion followed by nitrile reduction using sodium borohydride and aqueous nickel(II) chloride with 72% conversion. Final peptide coupling reaction followed by HPLC purification and formulation afforded ready-to-inject [ 18 F]DTG in 5.1 ± 0.8% ( n = 10) decay-corrected radiochemical yield within 95 min. The whole process was automatized using a TRACERlab ® FX NPro module, and quality control performed by analytical HPLC showed that [ 18 F]DTG was suitable for in vivo injection with >99% chemical and radiochemical purity and a molar activity of 83 ± 18 GBq/µmol ( n = 10). Whole-body distribution of [ 18 F]DTG was performed by PET imaging on a healthy macaque and highlighted the elimination routes of the tracer. This study demonstrated the feasibility of in vivo [ 18 F]DTG PET imaging and paved the way to explore drug/virus/tissues interactions in animals and humans.
    Schlagwörter fluorine-18 ; radiolabeling ; dolutegravir ; PET imaging ; Medicine ; R ; Pharmacy and materia medica ; RS1-441
    Thema/Rubrik (Code) 333
    Sprache Englisch
    Erscheinungsdatum 2022-05-01T00:00:00Z
    Verlag MDPI AG
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    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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