LIVIVO - Suchergebnisse -

Suchergebnis

Treffer 1 - 10 von insgesamt 16

Suchoptionen

Artikel ; Online: Detection of intrathecal IgG antibody for varicella and measles diagnosis by evaluation and comparison of a commercial IgG chemiluminescent immunoassay with two ELISAs.

Garcia, Rafael / Jiménez-Valera, Maria / Ruiz-Buck, Daniel / Sanchez, Carlos / Rojas, Almudena / Schütz, Malte Hendrik / Rojas, Jose / Hunfeld, Klaus- Peter

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

2024

Abstract: Objectives: Analyse alternative methods of intrathecal antibody detection by comparing chemiluminescent immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) techniques to determine if CLIA can replace ELISA in the diagnosis of CNS infections. ...

Abstract	Objectives: Analyse alternative methods of intrathecal antibody detection by comparing chemiluminescent immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) techniques to determine if CLIA can replace ELISA in the diagnosis of CNS infections. Methods: A panel of 280 paired samples-cerebrospinal fluid (CSF) and serum-with known antibody reactivities (Varicella, n = 60; Measles, n = 120) and negative samples (n = 100) were used to evaluate the performance of six serological test kits (Enzygnost, VirClia®, and Serion ELISA (Measles and Variella). Results: For Measles virus IgG, the VirClia® IgG monotest revealed 97% and 94% positive and negative agreement to the Enzygnost as reference test, respectively. In contrast, Serion ELISA kits yielded values of 18% and 90%. For the Varicella Zoster virus (VZV) IgG, the VirClia® IgG monotest showed 97% and 90% positive and negative agreement compared to Enzygnost. The Serion ELISA kits showed values of 55% and 86%, respectively. ROC analysis revealed that the areas under the curve for Measles and VZV IgGs were 0.7 and 0.852, respectively, using the Serion kit, and 0.963 and 0.955, for Vircell S.L CLIA technique. VirClia® monotest values were calculated using an antibody index cut-off of 1.3. Conclusion: The findings indicate that CLIA testing can improve antibody detection in CSF samples, aiding the diagnosis of infectious neurological impairments.
Sprache	Englisch
Erscheinungsdatum	2024-04-13
Erscheinungsland	Germany
Dokumenttyp	Journal Article
ZDB-ID	603155-9
ISSN	1435-4373 ; 0934-9723 ; 0722-2211
ISSN (online)	1435-4373
ISSN	0934-9723 ; 0722-2211
DOI	10.1007/s10096-024-04822-x
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Zs.A 1732: Hefte anzeigen

Standort:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Details ▾

Artikel: In vitro neutralizing activity of BNT162b2 mRNA‐induced antibodies against full B.1.351 SARS‐CoV‐2 variant

Serrano‐Conde, Esther / Leyva, Alba / Fuentes, Ana / de Salazar, Adolfo / Chueca, Natalia / Pérez‐Castro, Sonia / Regueiro, Benito / Rojas, Almudena / Mendoza, Joaquín / Rojas, Jose / García, Federico

Transboundary and emerging diseases. 2022 Sept., v. 69, no. 5

2022

Abstract: SARS‐CoV‐2 variation represents a serious challenge to current COVID‐19 vaccines. Recent reports suggest that B.1.351 and other variants may escape the neutralization activity of the antibodies generated by current vaccines. Ninety‐nine healthcare ... ...

Abstract	SARS‐CoV‐2 variation represents a serious challenge to current COVID‐19 vaccines. Recent reports suggest that B.1.351 and other variants may escape the neutralization activity of the antibodies generated by current vaccines. Ninety‐nine healthcare workers undertaking BNT162b2 mRNA vaccination were sampled at baseline, on the day of the second dose, and 14 days after the latter. Neutralization activity against SARS‐CoV‐2 B.1, B.1.1.7 and B.1.351 was investigated using a Vero‐E6 model. Eleven of the study participants had prior infection with SARS‐CoV‐2. Neutralization titers against the B.1 and the B.1.1.7 variants were not statistically different and were significantly higher than titers against the B.1.351 variant across pre‐exposed and non‐pre‐exposed vaccinated individuals (p < .01). While all vaccinated individuals presented neutralizing antibodies against B.1 and B 1.1.7 after the second dose, 14% were negative against B.1.351 and 76% had low titers (1/201/80). Pre‐exposed vaccinated individuals showed higher titers than non‐pre‐exposed after the first (median titers of 1/387 versus 1/28, respectively) and the second doses (1/995 versus 1/703, respectively). As high as 72% of the pre‐exposed vaccines presented titers >1/80 after a single dose, while only 11% of non‐exposed vaccinated individuals had titers >1/80. BNT162b2 mRNA‐induced antibodies show a lower in vitro neutralizing activity against B.1.351 variant compared to neutralization against B.1.1.7 or B.1 variants. Interestingly, for individuals pre‐exposed to SARS‐CoV‐2, one dose of BNT162b2 mRNA may be adequate to produce neutralizing antibodies against B.1.1.7 and B.1, while two doses of BNT162b2 mRNA provide optimal neutralizing antibody response against B.1.351 too.
Schlagwörter	COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; antibody formation ; health services ; models ; neutralization ; vaccination
Sprache	Englisch
Erscheinungsverlauf	2022-09
Umfang	p. 2649-2655.
Erscheinungsort	John Wiley & Sons, Ltd
Dokumenttyp	Artikel
Anmerkung	JOURNAL ARTICLE
ZDB-ID	2414822-2
ISSN	1865-1682 ; 1865-1674
ISSN (online)	1865-1682
ISSN	1865-1674
DOI	10.1111/tbed.14417
Datenquelle	NAL Katalog (AGRICOLA)

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Über subito bestellen

Details ▾
- Volltext online
- Kostenpflichtig bestellen

Artikel ; Online: The DNA methylation landscape of the root‐knot nematode‐induced pseudo‐organ, the gall, in Arabidopsis, is dynamic, contrasting over time, and critically important for successful parasitism

Silva, Ana Cláudia / Ruiz‐Ferrer, Virginia / Müller, Sebastian Y. / Pellegrin, Clement / Abril‐Urías, Patricia / Martínez‐Gómez, Ángela / Gómez‐Rojas, Almudena / Berenguer, Eduardo / Testillano, Pilar S. / Andrés, Maria Fe / Fenoll, C. / Eves‐van den Akker, Sebastian / Escobar, Carolina

New Phytologist. 2022 Dec., v. 236, no. 5 p.1888-1907

2022

Abstract: Root‐knot nematodes (RKNs) induce giant cells (GCs) within galls which are characterized by large‐scale gene repression at early stages. However, the epigenetic mechanism(s) underlying gene silencing is (are) still poorly characterized. DNA methylation ... ...

Abstract	Root‐knot nematodes (RKNs) induce giant cells (GCs) within galls which are characterized by large‐scale gene repression at early stages. However, the epigenetic mechanism(s) underlying gene silencing is (are) still poorly characterized. DNA methylation in Arabidopsis galls induced by Meloidogyne javanica was studied at crucial infection stages (3 d post‐infection (dpi) and 14 dpi) using enzymatic, cytological, and sequencing approaches. DNA methyltransferase mutants (met1, cmt2, cmt3, cmt2/3, drm1/2, ddc) and a DNA demethylase mutant (ros1), were analyzed for RKN resistance/tolerance, and galls were characterized by confocal microscopy and RNA‐seq. Early galls were hypermethylated, and the GCs were found to be the major contributors to this hypermethylation, consistent with the very high degree of gene repression they exhibit. By contrast, medium/late galls showed no global increase in DNA methylation compared to uninfected roots, but exhibited large‐scale redistribution of differentially methylated regions (DMRs). In line with these findings, it was also shown that DNA methylation and demethylation mutants showed impaired nematode reproduction and gall/GC‐development. Moreover, siRNAs that were exclusively present in early galls accumulated at hypermethylated DMRs, overlapping mostly with retrotransposons in the CHG/CG contexts that might be involved in their repression, contributing to their stability/genome integrity. Promoter/gene methylation correlated with differentially expressed genes encoding proteins with basic cell functions. Both mechanisms are consistent with reprogramming host tissues for gall/GC formation. In conclusion, RNA‐directed DNA methylation (RdDM; DRM2/1) pathways, maintenance methyltransferases (MET1/CMT3) and demethylation (ROS1) appear to be prominent mechanisms driving a dynamic regulation of the epigenetic landscape during RKN infection.
Schlagwörter	Arabidopsis ; DNA ; DNA methylation ; DNA methyltransferase ; Meloidogyne javanica ; confocal microscopy ; demethylation ; epigenetics ; gene expression regulation ; genes ; mutants ; parasitism ; reproduction ; retrotransposons ; root-knot nematodes ; sequence analysis
Sprache	Englisch
Erscheinungsverlauf	2022-12
Umfang	p. 1888-1907.
Erscheinungsort	John Wiley & Sons, Ltd
Dokumenttyp	Artikel ; Online
Anmerkung	JOURNAL ARTICLE
ZDB-ID	208885-x
ISSN	1469-8137 ; 0028-646X
ISSN (online)	1469-8137
ISSN	0028-646X
DOI	10.1111/nph.18395
Datenquelle	NAL Katalog (AGRICOLA)

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Verfügbar in ZB MED Bonn

Z 868: Hefte anzeigen

Über subito bestellen

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾

Artikel: Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis.

Abril-Urias, Patricia / Ruiz-Ferrer, Virginia / Cabrera, Javier / Olmo, Rocio / Silva, Ana Cláudia / Díaz-Manzano, Fernando Evaristo / Domínguez-Figueroa, Jose / Martínez-Gómez, Ángela / Gómez-Rojas, Almudena / Moreno-Risueno, Miguel Ángel / Fenoll, Carmen / Escobar, Carolina

Frontiers in plant science

2023 Band 14, Seite(n) 1024815

Abstract: Cysts (CNs) and root-knot nematodes (RKNs) induce specialized feeding cells, syncytia, and giant cells (GCs), respectively, within plant roots. The plant tissues around the GCs usually by respond forming a root swelling called a gall that contains the ... ...

Abstract	Cysts (CNs) and root-knot nematodes (RKNs) induce specialized feeding cells, syncytia, and giant cells (GCs), respectively, within plant roots. The plant tissues around the GCs usually by respond forming a root swelling called a gall that contains the GCs. The ontogenesis of feeding cells is different. GC formation is a process of new organogenesis from vascular cells, which are still not well characterized, that differentiate into GCs. In contrast, syncytia formation involves the fusion of adjacent cells that have already differentiated. Nonetheless, both feeding sites show an auxin maximum pertinent to feeding site formation. However, data on the molecular divergences and similarities between the formation of both feeding sites regarding auxin-responsive genes are still scarce. We studied genes from the auxin transduction pathways that are crucial during gall and lateral root (LR) development in the CN interaction by using promoter-reporter (GUS/LUC)transgenic lines, as well as loss of function lines of Arabidopsis. The promoters
Sprache	Englisch
Erscheinungsdatum	2023-02-15
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2613694-6
ISSN	1664-462X
ISSN	1664-462X
DOI	10.3389/fpls.2023.1024815
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Reduced neutralizing antibody response to SARS-CoV-2 vaccine booster dose in people living with HIV with severe immunosuppression.

Corma-Gómez, Anaïs / Fernández-Fuertes, Marta / Viñuela, Laura / Domínguez, Carmen / Santos, Marta / Fuentes-López, Ana / Rojas, Almudena / Fernández-Pérez, Nieves / Martín-Carmona, Jessica / Serrano-Conde, Esther / Real, Luis M / Mendoza, Joaquín / Macías, Juan / Pineda, Juan A / García, Federico

Journal of medical virology

2023 Band 95, Heft 3, Seite(n) e28602

Abstract: The aim of this study was to assess the immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines among people living with HIV (PLWH) with severe immunosuppression, after a booster dose. The design was a case-control study ... ...

Abstract	The aim of this study was to assess the immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines among people living with HIV (PLWH) with severe immunosuppression, after a booster dose. The design was a case-control study nested in a prospective cohort of PLWH. All patients with CD4 cell count <200 cells/mm
Mesh-Begriff(e)	Humans ; COVID-19 Vaccines ; Antibodies, Neutralizing ; Antibody Formation ; Case-Control Studies ; Prospective Studies ; COVID-19/prevention & control ; SARS-CoV-2 ; Immunosuppression Therapy ; RNA, Messenger ; HIV Infections ; Antibodies, Viral
Chemische Substanzen	COVID-19 Vaccines ; Antibodies, Neutralizing ; RNA, Messenger ; Antibodies, Viral
Sprache	Englisch
Erscheinungsdatum	2023-03-07
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.28602
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Zs.A 1320: Hefte anzeigen

Standort:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Details ▾

Artikel ; Online: In vitro neutralizing activity of BNT162b2 mRNA-induced antibodies against full B.1.351 SARS-CoV-2 variant.

Serrano-Conde, Esther / Leyva, Alba / Fuentes, Ana / de Salazar, Adolfo / Chueca, Natalia / Pérez-Castro, Sonia / Regueiro, Benito / Rojas, Almudena / Mendoza, Joaquín / Rojas, Jose / García, Federico

Transboundary and emerging diseases

2022 Band 69, Heft 5, Seite(n) 2649–2655

Abstract: SARS-CoV-2 variation represents a serious challenge to current COVID-19 vaccines. Recent reports suggest that B.1.351 and other variants may escape the neutralization activity of the antibodies generated by current vaccines. Ninety-nine healthcare ... ...

Abstract	SARS-CoV-2 variation represents a serious challenge to current COVID-19 vaccines. Recent reports suggest that B.1.351 and other variants may escape the neutralization activity of the antibodies generated by current vaccines. Ninety-nine healthcare workers undertaking BNT162b2 mRNA vaccination were sampled at baseline, on the day of the second dose, and 14 days after the latter. Neutralization activity against SARS-CoV-2 B.1, B.1.1.7 and B.1.351 was investigated using a Vero-E6 model. Eleven of the study participants had prior infection with SARS-CoV-2. Neutralization titers against the B.1 and the B.1.1.7 variants were not statistically different and were significantly higher than titers against the B.1.351 variant across pre-exposed and non-pre-exposed vaccinated individuals (p < .01). While all vaccinated individuals presented neutralizing antibodies against B.1 and B 1.1.7 after the second dose, 14% were negative against B.1.351 and 76% had low titers (1/201/80). Pre-exposed vaccinated individuals showed higher titers than non-pre-exposed after the first (median titers of 1/387 versus 1/28, respectively) and the second doses (1/995 versus 1/703, respectively). As high as 72% of the pre-exposed vaccines presented titers >1/80 after a single dose, while only 11% of non-exposed vaccinated individuals had titers >1/80. BNT162b2 mRNA-induced antibodies show a lower in vitro neutralizing activity against B.1.351 variant compared to neutralization against B.1.1.7 or B.1 variants. Interestingly, for individuals pre-exposed to SARS-CoV-2, one dose of BNT162b2 mRNA may be adequate to produce neutralizing antibodies against B.1.1.7 and B.1, while two doses of BNT162b2 mRNA provide optimal neutralizing antibody response against B.1.351 too.
Mesh-Begriff(e)	Animals ; Antibodies, Neutralizing ; Antibodies, Viral ; BNT162 Vaccine ; COVID-19/prevention & control ; COVID-19/veterinary ; COVID-19 Vaccines ; Humans ; Membrane Glycoproteins ; Neutralization Tests/veterinary ; RNA, Messenger/genetics ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus ; Viral Envelope Proteins/genetics
Chemische Substanzen	Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 Vaccines ; Membrane Glycoproteins ; RNA, Messenger ; Spike Glycoprotein, Coronavirus ; Viral Envelope Proteins ; spike protein, SARS-CoV-2 ; BNT162 Vaccine (N38TVC63NU)
Sprache	Englisch
Erscheinungsdatum	2022-01-03
Erscheinungsland	Germany
Dokumenttyp	Journal Article
ZDB-ID	2414822-2
ISSN	1865-1682 ; 1865-1674
ISSN (online)	1865-1682
ISSN	1865-1674
DOI	10.1111/tbed.14417
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Über subito bestellen

Details ▾
- Volltext online
- Kostenpflichtig bestellen

Artikel ; Online: The DNA methylation landscape of the root-knot nematode-induced pseudo-organ, the gall, in Arabidopsis, is dynamic, contrasting over time, and critically important for successful parasitism.

Silva, Ana Cláudia / Ruiz-Ferrer, Virginia / Müller, Sebastian Y / Pellegrin, Clement / Abril-Urías, Patricia / Martínez-Gómez, Ángela / Gómez-Rojas, Almudena / Berenguer, Eduardo / Testillano, Pilar S / Andrés, Maria Fe / Fenoll, Carmen / Eves-van den Akker, Sebastian / Escobar, Carolina

The New phytologist

2022 Band 236, Heft 5, Seite(n) 1888–1907

Abstract: Root-knot nematodes (RKNs) induce giant cells (GCs) within galls which are characterized by large-scale gene repression at early stages. However, the epigenetic mechanism(s) underlying gene silencing is (are) still poorly characterized. DNA methylation ... ...

Abstract	Root-knot nematodes (RKNs) induce giant cells (GCs) within galls which are characterized by large-scale gene repression at early stages. However, the epigenetic mechanism(s) underlying gene silencing is (are) still poorly characterized. DNA methylation in Arabidopsis galls induced by Meloidogyne javanica was studied at crucial infection stages (3 d post-infection (dpi) and 14 dpi) using enzymatic, cytological, and sequencing approaches. DNA methyltransferase mutants (met1, cmt2, cmt3, cmt2/3, drm1/2, ddc) and a DNA demethylase mutant (ros1), were analyzed for RKN resistance/tolerance, and galls were characterized by confocal microscopy and RNA-seq. Early galls were hypermethylated, and the GCs were found to be the major contributors to this hypermethylation, consistent with the very high degree of gene repression they exhibit. By contrast, medium/late galls showed no global increase in DNA methylation compared to uninfected roots, but exhibited large-scale redistribution of differentially methylated regions (DMRs). In line with these findings, it was also shown that DNA methylation and demethylation mutants showed impaired nematode reproduction and gall/GC-development. Moreover, siRNAs that were exclusively present in early galls accumulated at hypermethylated DMRs, overlapping mostly with retrotransposons in the CHG/CG contexts that might be involved in their repression, contributing to their stability/genome integrity. Promoter/gene methylation correlated with differentially expressed genes encoding proteins with basic cell functions. Both mechanisms are consistent with reprogramming host tissues for gall/GC formation. In conclusion, RNA-directed DNA methylation (RdDM; DRM2/1) pathways, maintenance methyltransferases (MET1/CMT3) and demethylation (ROS1) appear to be prominent mechanisms driving a dynamic regulation of the epigenetic landscape during RKN infection.
Mesh-Begriff(e)	Animals ; Arabidopsis/metabolism ; Protein-Tyrosine Kinases/genetics ; Protein-Tyrosine Kinases/metabolism ; Gene Expression Regulation, Plant ; DNA Methylation/genetics ; Plant Roots/genetics ; Plant Roots/metabolism ; Proto-Oncogene Proteins/genetics ; Proto-Oncogene Proteins/metabolism ; Tylenchoidea/physiology ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; DNA (Cytosine-5-)-Methyltransferases/genetics ; DNA (Cytosine-5-)-Methyltransferases/metabolism
Chemische Substanzen	Protein-Tyrosine Kinases (EC 2.7.10.1) ; Proto-Oncogene Proteins ; Arabidopsis Proteins ; MET1 protein, Arabidopsis (EC 2.1.1.-) ; DNA (Cytosine-5-)-Methyltransferases (EC 2.1.1.37)
Sprache	Englisch
Erscheinungsdatum	2022-09-02
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	208885-x
ISSN	1469-8137 ; 0028-646X
ISSN (online)	1469-8137
ISSN	0028-646X
DOI	10.1111/nph.18395
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Verfügbar in ZB MED Bonn

Z 868: Hefte anzeigen

Über subito bestellen

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾

Artikel: Vircell assays for detection of antibodies against Legionella pneumophila.

Rojas, Almudena / Rojas, José / Mendoza, Joaquín

Clinical and vaccine immunology : CVI

2007 Band 14, Heft 2, Seite(n) 208; author reply 208–9

Mesh-Begriff(e)	Antibodies, Bacterial/blood ; Enzyme-Linked Immunosorbent Assay ; Humans ; Legionella pneumophila/immunology ; Legionnaires' Disease/diagnosis ; Legionnaires' Disease/immunology
Chemische Substanzen	Antibodies, Bacterial
Sprache	Englisch
Erscheinungsdatum	2007-02
Erscheinungsland	United States
Dokumenttyp	Comment ; Comparative Study ; Letter ; Validation Studies
ZDB-ID	2221082-9
ISSN	1556-679X ; 1556-6811
ISSN (online)	1556-679X
ISSN	1556-6811
DOI	10.1128/CVI.00128-06
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Volltext online

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Zs.A 4000: Hefte anzeigen

Standort:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Details ▾

Artikel ; Online: Development of an enzyme-linked immunosorbent assay-based test with a cocktail of nucleocapsid and spike proteins for detection of severe acute respiratory syndrome-associated coronavirus-specific antibody.

Giménez, Luis G / Rojas, Jose / Rojas, Almudena / Mendoza, Joaquín / Camacho, Ana G

Clinical and vaccine immunology : CVI

2008 Band 16, Heft 2, Seite(n) 241–245

Abstract: A new enzyme-linked immunosorbent assay (ELISA)-based immunoglobulin G (IgG)-plus-IgM antibody detection test for severe acute respiratory syndrome (SARS) has been developed by using a cocktail of four recombinant polypeptides as the antigen. These ... ...

Abstract	A new enzyme-linked immunosorbent assay (ELISA)-based immunoglobulin G (IgG)-plus-IgM antibody detection test for severe acute respiratory syndrome (SARS) has been developed by using a cocktail of four recombinant polypeptides as the antigen. These recombinant fragments were designed as parts of two different structural proteins from SARS-associated coronavirus (SARS-CoV). One recombinant polypeptide, S251-683, was designed as part of the spike glycoprotein, and the other three polypeptides comprised almost the whole nucleocapsid protein, avoiding the last 25 C-terminal amino acids. Immunization with a cocktail of these four polypeptides yielded a specific polyclonal antibody that is able to recognize SARS-CoV-infected cells by an immunofluorescence assay. This polypeptide cocktail was also used to set up an ELISA-based IgG-plus-IgM antibody detection test, which showed 99% specificity and 90% sensitivity upon evaluation using sera from 100 healthy negative controls and 20 SARS patients. Separate immunoreactivity assays with each recombinant polypeptide demonstrated that a combination of N and S protein fragments was more suitable than the individual peptides for developing a serological assay for SARS-CoV.
Mesh-Begriff(e)	Antibodies, Viral/blood ; Antigens, Viral ; Coronavirus Nucleocapsid Proteins ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Membrane Glycoproteins/immunology ; Nucleocapsid Proteins/immunology ; Recombinant Proteins ; SARS Virus/immunology ; Sensitivity and Specificity ; Severe Acute Respiratory Syndrome/diagnosis ; Spike Glycoprotein, Coronavirus ; Viral Envelope Proteins/immunology
Chemische Substanzen	Antibodies, Viral ; Antigens, Viral ; Coronavirus Nucleocapsid Proteins ; Immunoglobulin G ; Immunoglobulin M ; Membrane Glycoproteins ; Nucleocapsid Proteins ; Recombinant Proteins ; Spike Glycoprotein, Coronavirus ; Viral Envelope Proteins ; spike glycoprotein, SARS-CoV ; spike protein, mouse hepatitis virus
Schlagwörter	covid19
Sprache	Englisch
Erscheinungsdatum	2008-11-26
Erscheinungsland	United States
Dokumenttyp	Evaluation Study ; Journal Article
ZDB-ID	2221082-9
ISSN	1556-679X ; 1556-6811
ISSN (online)	1556-679X
ISSN	1556-6811
DOI	10.1128/CVI.00252-08
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Zs.A 4000: Hefte anzeigen

Standort:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Details ▾
- Im ZB MED-Bestand
- Kostenpflichtig bestellen

Konferenzbeitrag: SARS-associated coronavirus diagnostic kit: development of 4 recombinant polypeptides and a polyclonal Ab for direct and indirect virus detection

Camacho, Ana / Rojas, Jose / Rojas, Almudena / Mendoza, Joaquín

2004 , Seite(n) 04sarsP8.01

Veranstaltung/Kongress	International Conference on SARS - one year after the (first) outbreak; Lübeck; 2004
Schlagwörter	Medizin, Gesundheit
Erscheinungsdatum	2004-05-26
Verlag	German Medical Science; Düsseldorf, Köln
Dokumenttyp	Konferenzbeitrag
Datenquelle	German Medical Science

Zusatzmaterialien

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾

Zum Seitenanfang

Ihre letzten Suchen

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Bonn

Über subito bestellen

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Bonn

Über subito bestellen

Fernleihe an ZB MED

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Zusatzmaterialien

Kategorien

Fernleihe an ZB MED