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  1. AU="Rollins, Alicia"
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  1. Artikel: SARS-CoV-2 serosurvey of healthy, privately owned cats presenting to a New York City animal hospital in the early phase of the COVID-19 pandemic (2020-2021).

    Choi, Annette / Stout, Alison E / Rollins, Alicia / Wang, Kally / Guo, Qinghua / Javier, A Jaimes / Kennedy, Monica / Wagner, Bettina / Whittaker, Gary R

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Both domestic and non-domestic cats are now established to be susceptible to infection by SARS-CoV-2, the cause of the ongoing COVID-19 pandemic. While serious disease in cats may occur in some instances, the majority of infections appear to be ... ...

    Abstract Both domestic and non-domestic cats are now established to be susceptible to infection by SARS-CoV-2, the cause of the ongoing COVID-19 pandemic. While serious disease in cats may occur in some instances, the majority of infections appear to be subclinical. Differing prevalence data for SARS-CoV-2 infection of cats have been reported, and are highly context-dependent. Here, we report a retrospective serological survey of cats presented to an animal practice in New York City, located in close proximity to a large medical center that treated the first wave of COVID-19 patients in the US in the Spring of 2020. We sampled 79, mostly indoor, cats between June 2020 to May 2021, the early part of which time the community was under a strict public health "lock-down". Using a highly sensitive and specific fluorescent bead-based multiplex assay, we found an overall prevalence of 13/79 (16%) serologically-positive animals for the study period; however, cats sampled in the Fall of 2020 had a confirmed positive prevalence of 44%. For SARS-CoV-2 seropositive cats, we performed viral neutralization test with live SARS-CoV-2 to additionally confirm presence of SARS-CoV-2 specific antibodies. Of the thirteen seropositive cats, 7/13 (54%) were also positive by virus neutralization, and 2 of seropositive cats had previously documented respiratory signs, with high neutralization titers of 1:1024 and 1:4096; overall however, there was no statistically significant association of SARS-CoV-2 seropositivity with respiratory signs, or with breed, sex or age of the animals. Follow up sampling of cats, while limited in scope, showed that positive serological titers were maintained over time. In comparison, we found an overall confirmed positive prevalence of 51% for feline coronavirus (FCoV), an endemic virus of cats, with 30% confirmed negative for FCoV. We demonstrate the impact of SARS-CoV in a defined feline population during the first wave of SARS-CoV-2 infection of humans, and suggest that human-cat transmission was substantial in our study group. Our data provide a new context for SARS-CoV-2 transmission events across species.
    Sprache Englisch
    Erscheinungsdatum 2024-02-15
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2024.02.13.580068
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Inflammatory markers for differentiation of endometritis in the mare.

    Lection, Jennine / Wagner, Bettina / Byron, Michael / Miller, Andrew / Rollins, Alicia / Chenier, Tracey / Cheong, Soon Hon / Diel de Amorim, Mariana

    Equine veterinary journal

    2024  

    Abstract: Background: Endometrial biopsy is required to diagnose mares with chronic endometritis and endometrial degenerative fibrosis. An increase in understanding of equine reproductive immunology could be utilised to create less-invasive, time-efficient ... ...

    Abstract Background: Endometrial biopsy is required to diagnose mares with chronic endometritis and endometrial degenerative fibrosis. An increase in understanding of equine reproductive immunology could be utilised to create less-invasive, time-efficient diagnostic tools especially when evaluating mares for chronic endometritis.
    Objectives: To evaluate inflammatory cytokine and chemokine concentrations in uterine fluid samples collected by low-volume lavage (LVL) as a potential screening diagnostic biomarker for endometritis.
    Study design: Prospective cross-sectional clinical study.
    Methods: Forty-six mares underwent a LVL and subsequently endometrial biopsy. Mares were split in three groups: healthy, acute endometritis, and chronic endometrial fibrosis (CEF) based on cytological and histological evaluation. A fluorescent bead-based multiplex assay for IFN-γ, IFN-α, IL-1β, IL-4, IL-10, IL-17, sCD14, TNF-α, CCL2, CCL3, CCL5 and CCL11 were carried out on the LVL fluid. The endometrial biopsy was utilised for histology and qPCR of IFN-γ, IL-1β, IL-6, IL-8, IL-17, TNF-α, CCL2 and CCL3 genes. Statistical analyses examined differences in inflammatory markers and predictive modelling for diseased endometrium.
    Results: Secreted concentrations of IFN-γ were lower in LVL fluid from reproductively healthy mares compared with acute endometritis (p = 0.04) and CEF (p = 0.006). Additionally, IL-17, IL-10, IL-1β, TNF-α, CCL2, CCL3, CCL5 and CCL11 were significantly increased (p ≤ 0.04) in LVL from CEF mares compared with healthy mares. Mares with CCL2 concentrations ≥550 pg/mL (14/14) had 100% probability of having CEF and/or acute endometritis. Healthy mares had lower relative abundance of IL-17 mRNA compared with mares in CEF group [median (interquartile rage) = 14.76 (13.3, 15.3) and 12.4 (10.54, 13.81)], respectively (p = 0.02).
    Main limitations: Limited sample size: larger numbers of mares with and without endometritis are required and reference intervals in LVL samples have to be established.
    Conclusions: Inflammatory chemokines and cytokines concentrations differed between healthy mares and mares with acute endometritis or CEF in LVL.
    Sprache Englisch
    Erscheinungsdatum 2024-01-14
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 41606-x
    ISSN 2042-3306 ; 0425-1644
    ISSN (online) 2042-3306
    ISSN 0425-1644
    DOI 10.1111/evj.14058
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: SARS-CoV-2 serosurvey of healthy, privately owned cats presenting to a New York City animal hospital in the early phase of the COVID-19 pandemic (2020-2021)

    Choi, Annette / Stout, Alison E. / Rollins, Alicia / Wang, Kally / Guo, Qinghua / Javier, A. Jaimes / Kennedy, Monica / Wagner, Bettina / Whittaker, Gary R.

    bioRxiv

    Abstract: Both domestic and non-domestic cats are now established to be susceptible to infection by SARS-CoV-2, the cause of the ongoing COVID-19 pandemic. While serious disease in cats may occur in some instances, the majority of infections appear to be ... ...

    Abstract Both domestic and non-domestic cats are now established to be susceptible to infection by SARS-CoV-2, the cause of the ongoing COVID-19 pandemic. While serious disease in cats may occur in some instances, the majority of infections appear to be subclinical. Differing prevalence data for SARS-CoV-2 infection of cats have been reported, and are highly context-dependent. Here, we report a retrospective serological survey of cats presented to an animal practice in New York City, located in close proximity to a large medical center that treated the first wave of COVID-19 patients in the US in the Spring of 2020. We sampled 79, mostly indoor, cats between June 2020 to May 2021, the early part of which time the community was under a strict public health lock-down. Using a highly sensitive and specific fluorescent bead-based multiplex assay, we found an overall prevalence of 13/79 (16%) serologically-positive animals for the study period; however, cats sampled in the Fall of 2020 had a confirmed positive prevalence of 44%. For SARS-CoV-2 seropositive cats, we performed viral neutralization test with live SARS-CoV-2 to additionally confirm presence of SARS-CoV-2 specific antibodies. Of the thirteen seropositive cats, 7/13 (54%) were also positive by virus neutralization, and 2 of seropositive cats had previously documented respiratory signs, with high neutralization titers of 1:1024 and 1:4096; overall however, there was no statistically significant association of SARS-CoV-2 seropositivity with respiratory signs, or with breed, sex or age of the animals. Follow up sampling of cats, while limited in scope, showed that positive serological titers were maintained over time. In comparison, we found an overall confirmed positive prevalence of 51% for feline coronavirus (FCoV), an endemic virus of cats, with 30% confirmed negative for FCoV. We demonstrate the impact of SARS-CoV in a defined feline population during the first wave of SARS-CoV-2 infection of humans, and suggest that human-cat transmission was substantial in our study group. Our data provide a new context for SARS-CoV-2 transmission events across species.
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2024-02-15
    Verlag Cold Spring Harbor Laboratory
    Dokumenttyp Artikel ; Online
    DOI 10.1101/2024.02.13.580068
    Datenquelle COVID19

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  4. Artikel ; Online: SARS-CoV-2 serosurvey of healthy, privately owned cats presenting to a New York City animal hospital in the early phase of the COVID-19 pandemic (2020-2021)

    Choi, Annette / Stout, Alison E. / Rollins, Alicia / Wang, Kally / Guo, Qinghua / Jaimes, Javier A. / Kennedy, Monica / Wagner, Bettina / Whittaker, Gary R.

    bioRxiv

    Abstract: Both domestic and non-domestic cats are now established to be susceptible to infection by SARS-CoV-2, the cause of the ongoing COVID-19 pandemic. While serious disease in cats may occur in some instances, the majority of infections appear to be ... ...

    Abstract Both domestic and non-domestic cats are now established to be susceptible to infection by SARS-CoV-2, the cause of the ongoing COVID-19 pandemic. While serious disease in cats may occur in some instances, the majority of infections appear to be subclinical. Differing prevalence data for SARS-CoV-2 infection of cats have been reported, and are highly context-dependent. Here, we report a retrospective serological survey of cats presented to an animal practice in New York City, located in close proximity to a large medical center that treated the first wave of COVID-19 patients in the US in the Spring of 2020. We sampled 79, mostly indoor, cats between June 2020 to May 2021, the early part of which time the community was under a strict public health lock-down. Using a highly sensitive and specific fluorescent bead-based multiplex assay, we found an overall prevalence of 13/79 (16%) serologically-positive animals for the study period; however, cats sampled in the Fall of 2020 had a confirmed positive prevalence of 44%. For SARS-CoV-2 seropositive cats, we performed viral neutralization test with live SARS-CoV-2 to additionally confirm presence of SARS-CoV-2 specific antibodies. Of the thirteen seropositive cats, 7/13 (54%) were also positive by virus neutralization, and 2 of seropositive cats had previously documented respiratory signs, with high neutralization titers of 1:1024 and 1:4096; overall however, there was no statistically significant association of SARS-CoV-2 seropositivity with respiratory signs, or with breed, sex or age of the animals. Follow up sampling of cats, while limited in scope, showed that positive serological titers were maintained over time. In comparison, we found an overall confirmed positive prevalence of 51% for feline coronavirus (FCoV), an endemic virus of cats, with 30% confirmed negative for FCoV. We demonstrate the impact of SARS-CoV in a defined feline population during the first wave of SARS-CoV-2 infection of humans, and suggest that human-cat transmission was substantial in our study group. Our data provide a new context for SARS-CoV-2 transmission events across species.
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2024-02-15
    Verlag Cold Spring Harbor Laboratory
    Dokumenttyp Artikel ; Online
    DOI 10.1101/2024.02.13.580068
    Datenquelle COVID19

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  5. Artikel ; Online: Use of endometrial swab and cytobrush for detection of inflammatory diagnostic markers of endometritis

    Lection, Jennine / Schnobrich, Maria / Wagner, Bettina / Byron, Michael / Back, Bradley / Rollins, Alicia / Cheong, Soon Hon / de Amorim, Mariana Diel

    Journal of Equine Veterinary Science. 2023 June, v. 125 p.104725-

    2023  

    Abstract: Screening methods for endometritis are often performed through endometrial culture and cytology with a variety of techniques including endometrial swab, cytobrush, low-volume lavage, or biopsy. With growing research interest in equine reproductive ... ...

    Abstract Screening methods for endometritis are often performed through endometrial culture and cytology with a variety of techniques including endometrial swab, cytobrush, low-volume lavage, or biopsy. With growing research interest in equine reproductive immunology and low sensitivity of current screening tools for endometritis, one area that would be of immediate benefit to clinicians and clients is inflammatory diagnostic development. Previous research detected inflammatory markers in low-volume lavage fluid in mares with chronic and acute endometritis (Lection et al. AAEP Proceedings. 2020; 66: 150-151). Therefore, our objective was to investigate the utility of the endometrial swab and cytobrush to screen mares for endometritis. Mares (n=84) of reproductive age ranging 3-20 years of age had an endometrial swab (n=103) and/or followed by cytobrush (n=94) taken once in the same estrous cycle and placed in Amies media (n=172) or PBS (n=25). Aerobic culture was performed in 139 of those samples. An endometrial biopsy was taken on a subset of mares (n=46) and graded by a board-certified veterinary pathologist. The media in which the cytobrushes and swabs were stored was used in a bead-based multiplex assay (Luminex Corp. Austin, TX) to quantify concentration of the following equine specific biomarkers: IFN-α, IFN-γ, IL-1β, IL-4, IL-10, IL-17, sCD14, TNF-α, CCL2, CCL3, CCL5, and CCL11. A Shapiro-Wilk test was used to check normality, followed by a Wilcoxon testperformed in JMP Pro 16 (Cary, NC) to assess for significant differences in inflammatory marker concentrations between healthy mares and those with either cytology-diagnosed endometritis (≥1 neutrophil/hpf), positive bacterial culture, or poor biopsy scores (IIB or III on the Kenney-Doig scale). IFN-γ (P≤0.02) and IL-17 (P≤0.04) were significantly increased in mares with poor biopsy scores in both swab and cytobrush compared to mares with biopsy I or IIA. Mares with inflammation on cytology showed increased concentration of IL-1β (P≤0.05) and decreased levels of IFN-γ (P≤0.02) and CCL5 (P≤0.03) in swab. Mares with intrauterine fluid on ultrasound had increased IL-17 (P≤0.05) on cytobrush. Bacterial growth was detected in 62/139 samples, with 31 of those being gram-positive. sCD14 and TNF-α tended to be increased in cytobrush samples from mares with a positive culture, and with gram-positive bacteria compared to gram-negative, respectively (P=0.06). Cytokine levels significantly differed between cytobrush and swab for IFN-γ (P≤0.004) with increased levels for cytobrush, likely due to the higher amount of cellular exfoliation. Several inflammatory markers show promise as an ancillary screening test for equine endometritis. In conclusion, as equine reproductive immunology expands as a field, our understanding of patterns of inflammatory cytokines and chemokines can allow for new diagnostic techniques for endometritis in mares.
    Schlagwörter bacterial culture ; bacterial growth ; biomarkers ; biopsy ; chemokines ; endometritis ; endometrium ; estrous cycle ; horses ; inflammation ; interleukin-10 ; interleukin-17 ; interleukin-4 ; neutrophils ; ultrasonics ; veterinary medicine
    Sprache Englisch
    Erscheinungsverlauf 2023-06
    Erscheinungsort Elsevier Inc.
    Dokumenttyp Artikel ; Online
    ZDB-ID 2102631-2
    ISSN 1542-7412 ; 0737-0806
    ISSN (online) 1542-7412
    ISSN 0737-0806
    DOI 10.1016/j.jevs.2023.104725
    Datenquelle NAL Katalog (AGRICOLA)

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  6. Artikel: Intranasal IgG4/7 antibody responses protect horses against equid herpesvirus-1 (EHV-1) infection including nasal virus shedding and cell-associated viremia

    Perkins, Gillian / Babasyan, Susanna / Stout, Alison E / Freer, Heather / Rollins, Alicia / Wimer, Christine L / Wagner, Bettina

    Virology. 2019 May, v. 531

    2019  

    Abstract: Equid herpesvirus-1 (EHV-1) outbreaks continue despite widely used vaccination. We demonstrated previously that an ORF1/ORF71 gene deletion mutant of the EHV-1 strain Ab4 (Ab4ΔORF1/71) is less virulent than its parent Ab4 virus. Here, we describe the Ab4 ...

    Abstract Equid herpesvirus-1 (EHV-1) outbreaks continue despite widely used vaccination. We demonstrated previously that an ORF1/ORF71 gene deletion mutant of the EHV-1 strain Ab4 (Ab4ΔORF1/71) is less virulent than its parent Ab4 virus. Here, we describe the Ab4 challenge infection evaluating protection induced by the Ab4ΔORF1/71 vaccine candidate. Susceptible control horses developed respiratory disease, fever, nasal shedding, and viremia. Full protection after challenge infection was observed in 5/5 previously Ab4 infected horses and 3/5 Ab4ΔORF1/71 horses. Two Ab4ΔORF1/71 horses developed short-lasting viremia and/or virus shedding. Protective immunity in the respiratory tract was characterized by pre-existing EHV-1-specific IgG4/7 antibodies, the absence of IFN-α secretion and rapidly increasing IgG4/7 upon challenge infection. Pre-existing systemic EHV-1-specific IgG4/7 highly correlated with protection. T-cell immunity was overall low. In conclusion, protective immunity against EHV-1 infection including prevention of viremia was associated with robust systemic and intranasal IgG4/7 antibodies suggesting immediate virus neutralization at the local site.
    Schlagwörter Equid alphaherpesvirus 1 ; T-lymphocytes ; antibodies ; fever ; gene deletion ; horses ; immunoglobulin G ; interferon-alpha ; mutants ; neutralization tests ; nose ; respiratory tract diseases ; secretion ; vaccination ; vaccines ; viral shedding ; viremia ; virulence ; viruses
    Sprache Englisch
    Erscheinungsverlauf 2019-05
    Umfang p. 219-232.
    Erscheinungsort Elsevier Inc.
    Dokumenttyp Artikel
    ZDB-ID 200425-2
    ISSN 1096-0341 ; 0042-6822
    ISSN (online) 1096-0341
    ISSN 0042-6822
    DOI 10.1016/j.virol.2019.03.014
    Datenquelle NAL Katalog (AGRICOLA)

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    Z 3686: Hefte anzeigen

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  7. Artikel ; Online: Development of a quantitative COVID-19 multiplex assay and its use for serological surveillance in a low SARS-CoV-2 incidence community.

    Guarino, Cassandra / Larson, Elisabeth / Babasyan, Susanna / Rollins, Alicia / Joshi, Lok R / Laverack, Melissa / Parrilla, Lara / Plocharczyk, Elizabeth / Diel, Diego G / Wagner, Bettina

    PloS one

    2022  Band 17, Heft 1, Seite(n) e0262868

    Abstract: A serological COVID-19 Multiplex Assay was developed and validated using serum samples from convalescent patients and those collected prior to the 2020 pandemic. After initial testing of multiple potential antigens, the SARS-CoV-2 nucleocapsid protein ( ... ...

    Abstract A serological COVID-19 Multiplex Assay was developed and validated using serum samples from convalescent patients and those collected prior to the 2020 pandemic. After initial testing of multiple potential antigens, the SARS-CoV-2 nucleocapsid protein (NP) and receptor-binding domain (RBD) of the spike protein were selected for the human COVID-19 Multiplex Assay. A comparison of synthesized and mammalian expressed RBD proteins revealed clear advantages of mammalian expression. Antibodies directed against NP strongly correlated with SARS-CoV-2 virus neutralization assay titers (rsp = 0.726), while anti-RBD correlation was moderate (rsp = 0.436). Pan-Ig, IgG, IgA, and IgM against NP and RBD antigens were evaluated on the validation sample sets. Detection of NP and RBD specific IgG and IgA had outstanding performance (AUC > 0.90) for distinguishing patients from controls, but the dynamic range of the IgG assay was substantially greater. The COVID-19 Multiplex Assay was utilized to identify seroprevalence to SARS-CoV-2 in people living in a low-incidence community in Ithaca, NY. Samples were taken from a cohort of healthy volunteers (n = 332) in early June 2020. Only two volunteers had a positive result on a COVID-19 PCR test performed prior to serum sampling. Serological testing revealed an exposure rate of at least 1.2% (NP) or as high as 5.7% (RBD), higher than the measured incidence rate of 0.16% in the county at that time. This highly sensitive and quantitative assay can be used for monitoring community exposure rates and duration of immune response following both infection and vaccination.
    Mesh-Begriff(e) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies, Neutralizing/chemistry ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/chemistry ; Antibodies, Viral/immunology ; COVID-19/blood ; COVID-19/diagnosis ; COVID-19/epidemiology ; COVID-19 Serological Testing/methods ; COVID-19 Serological Testing/standards ; Coronavirus Nucleocapsid Proteins/chemistry ; Coronavirus Nucleocapsid Proteins/immunology ; Epidemiological Monitoring ; Female ; Humans ; Immunoglobulin A/chemistry ; Immunoglobulin A/immunology ; Immunoglobulin G/chemistry ; Immunoglobulin G/immunology ; Immunoglobulin M/chemistry ; Immunoglobulin M/immunology ; Male ; Middle Aged ; New York/epidemiology ; Phosphoproteins/chemistry ; Phosphoproteins/immunology ; Protein Interaction Domains and Motifs ; Recombinant Proteins/chemistry ; Recombinant Proteins/immunology ; SARS-CoV-2/classification ; SARS-CoV-2/immunology ; Sensitivity and Specificity ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/immunology
    Chemische Substanzen Antibodies, Neutralizing ; Antibodies, Viral ; Coronavirus Nucleocapsid Proteins ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Phosphoproteins ; Recombinant Proteins ; Spike Glycoprotein, Coronavirus ; nucleocapsid phosphoprotein, SARS-CoV-2 ; spike protein, SARS-CoV-2
    Sprache Englisch
    Erscheinungsdatum 2022-01-21
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0262868
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel: Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models

    Watkins, Amanda / Fasanello, Diana / Stefanovski, Darko / Schurer, Sydney / Caracappa, Katherine / D’Agostino, Albert / Costello, Emily / Freer, Heather / Rollins, Alicia / Read, Claire / Su, Jin / Colville, Marshall / Paszek, Matthew / Wagner, Bettina / Reesink, Heidi

    BMC veterinary research. 2021 Dec., v. 17, no. 1

    2021  

    Abstract: BACKGROUND: Lameness is a debilitating condition in equine athletes that leads to more performance limitation and loss of use than any other medical condition. There are a limited number of non-terminal experimental models that can be used to study early ...

    Abstract BACKGROUND: Lameness is a debilitating condition in equine athletes that leads to more performance limitation and loss of use than any other medical condition. There are a limited number of non-terminal experimental models that can be used to study early inflammatory and synovial fluid biophysical changes that occur in the equine joint. Here, we compare the well-established carpal IL-1β-induced synovitis model to a tarsal intra-articular lavage model, focusing on serial changes in synovial fluid inflammatory cytokines/chemokines and the synovial fluid lubricating molecules lubricin/proteoglycan 4 and hyaluronic acid. The objectives of this study were to evaluate clinical signs; synovial membrane and synovial fluid inflammation; and synovial fluid lubricants and biophysical properties in response to carpal IL-1β synovitis and tarsal intra-articular lavage. RESULTS: Hyaluronic acid (HA) concentrations, especially high molecular weight HA, and synovial fluid viscosity decreased after both synovitis and lavage interventions. Synovial fluid lubricin concentrations increased 17–20-fold for both synovitis and lavage models, with similar changes in both affected and contralateral joints, suggesting that repeated arthrocentesis alone resulted in elevated synovial fluid lubricin concentrations. Synovitis resulted in a more severe inflammatory response based on clinical signs (temperature, heart rate, respiratory rate, lameness and joint effusion) and clinicopathological and biochemical parameters (white blood cell count, total protein, prostaglandin E₂, sulfated glycosaminoglycans, tumor necrosis factor-α and CC chemokine ligands − 2, − 3, − 5 and − 11) as compared to lavage. CONCLUSIONS: Synovial fluid lubricin increased in response to IL-1β synovitis and joint lavage but also as a result of repeated arthrocentesis. Frequent repeated arthrocentesis is associated with inflammatory changes, including increased sulfated glycosaminoglycan concentrations and decreased hyaluronic acid concentrations. Synovitis results in more significant inflammatory changes than joint lavage. Our data suggests that synovial fluid lubricin, TNF-α, CCL2, CCL3, CCL5, CCL11 and sGAG may be useful biomarkers for synovitis and post-lavage joint inflammation. Caution should be exercised when performing repeated arthrocentesis clinically or in experimental studies due to the inflammatory response and loss of HA and synovial fluid viscosity.
    Schlagwörter biomarkers ; heart rate ; horses ; hyaluronic acid ; inflammation ; lameness ; leukocyte count ; ligands ; models ; molecular weight ; necrosis ; neoplasms ; prostaglandins ; protein content ; proteoglycans ; respiratory rate ; synovial fluid ; synovitis ; temperature ; veterinary medicine ; viscosity
    Sprache Englisch
    Erscheinungsverlauf 2021-12
    Umfang p. 189.
    Erscheinungsort BioMed Central
    Dokumenttyp Artikel
    ISSN 1746-6148
    DOI 10.1186/s12917-021-02873-2
    Datenquelle NAL Katalog (AGRICOLA)

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  9. Artikel ; Online: An Equine Herpesvirus Type 1 (EHV-1) Ab4 Open Reading Frame 2 Deletion Mutant Provides Immunity and Protection from EHV-1 Infection and Disease.

    Schnabel, Christiane L / Babasyan, Susanna / Rollins, Alicia / Freer, Heather / Wimer, Christine L / Perkins, Gillian A / Raza, Fahad / Osterrieder, Nikolaus / Wagner, Bettina

    Journal of virology

    2019  Band 93, Heft 22

    Abstract: Equine herpesvirus type 1 (EHV-1) outbreaks continue to occur despite widely used vaccination. Therefore, development of EHV-1 vaccines providing improved immunity and protection is ongoing. Here, an open reading frame 2 deletion mutant of the ... ...

    Abstract Equine herpesvirus type 1 (EHV-1) outbreaks continue to occur despite widely used vaccination. Therefore, development of EHV-1 vaccines providing improved immunity and protection is ongoing. Here, an open reading frame 2 deletion mutant of the neuropathogenic EHV-1 strain Ab4 (Ab4ΔORF2) was tested as a vaccine candidate. Three groups of horses (
    Mesh-Begriff(e) Administration, Intranasal/methods ; Animals ; Antibodies, Viral ; Female ; Herpesviridae Infections/virology ; Herpesvirus 1, Equid/genetics ; Herpesvirus 1, Equid/immunology ; Herpesvirus 1, Equid/metabolism ; Herpesvirus Vaccines/immunology ; Horse Diseases/virology ; Horses ; Male ; Nasal Mucosa/virology ; Open Reading Frames ; Rhadinovirus/immunology ; Vaccination/veterinary ; Viremia/immunology ; Virulence ; Virus Shedding/immunology
    Chemische Substanzen Antibodies, Viral ; Herpesvirus Vaccines
    Sprache Englisch
    Erscheinungsdatum 2019-10-29
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.01011-19
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Intranasal IgG4/7 antibody responses protect horses against equid herpesvirus-1 (EHV-1) infection including nasal virus shedding and cell-associated viremia.

    Perkins, Gillian / Babasyan, Susanna / Stout, Alison E / Freer, Heather / Rollins, Alicia / Wimer, Christine L / Wagner, Bettina

    Virology

    2019  Band 531, Seite(n) 219–232

    Abstract: Equid herpesvirus-1 (EHV-1) outbreaks continue despite widely used vaccination. We demonstrated previously that an ORF1/ORF71 gene deletion mutant of the EHV-1 strain Ab4 (Ab4ΔORF1/71) is less virulent than its parent Ab4 virus. Here, we describe the Ab4 ...

    Abstract Equid herpesvirus-1 (EHV-1) outbreaks continue despite widely used vaccination. We demonstrated previously that an ORF1/ORF71 gene deletion mutant of the EHV-1 strain Ab4 (Ab4ΔORF1/71) is less virulent than its parent Ab4 virus. Here, we describe the Ab4 challenge infection evaluating protection induced by the Ab4ΔORF1/71 vaccine candidate. Susceptible control horses developed respiratory disease, fever, nasal shedding, and viremia. Full protection after challenge infection was observed in 5/5 previously Ab4 infected horses and 3/5 Ab4ΔORF1/71 horses. Two Ab4ΔORF1/71 horses developed short-lasting viremia and/or virus shedding. Protective immunity in the respiratory tract was characterized by pre-existing EHV-1-specific IgG4/7 antibodies, the absence of IFN-α secretion and rapidly increasing IgG4/7 upon challenge infection. Pre-existing systemic EHV-1-specific IgG4/7 highly correlated with protection. T-cell immunity was overall low. In conclusion, protective immunity against EHV-1 infection including prevention of viremia was associated with robust systemic and intranasal IgG4/7 antibodies suggesting immediate virus neutralization at the local site.
    Mesh-Begriff(e) Administration, Intranasal ; Animals ; Antibodies, Viral/immunology ; Female ; Herpesviridae Infections/immunology ; Herpesviridae Infections/prevention & control ; Herpesviridae Infections/veterinary ; Herpesviridae Infections/virology ; Herpesvirus 1, Equid/drug effects ; Herpesvirus 1, Equid/genetics ; Herpesvirus 1, Equid/immunology ; Herpesvirus 1, Equid/physiology ; Herpesvirus Vaccines/administration & dosage ; Herpesvirus Vaccines/immunology ; Horse Diseases/immunology ; Horse Diseases/prevention & control ; Horse Diseases/virology ; Horses ; Immunoglobulin G/immunology ; Male ; Nasal Mucosa/immunology ; Nasal Mucosa/virology ; Vaccination ; Viremia/immunology ; Viremia/prevention & control ; Viremia/veterinary ; Viremia/virology ; Virus Shedding
    Chemische Substanzen Antibodies, Viral ; Herpesvirus Vaccines ; Immunoglobulin G
    Sprache Englisch
    Erscheinungsdatum 2019-03-22
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 200425-2
    ISSN 1096-0341 ; 0042-6822
    ISSN (online) 1096-0341
    ISSN 0042-6822
    DOI 10.1016/j.virol.2019.03.014
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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