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Article ; Online: Epidemiology and factors associated with Extra-pulmonary tuberculosis in a Low-prevalence area.

Rolo, M / González-Blanco, B / Reyes, C A / Rosillo, N / López-Roa, P

Journal of clinical tuberculosis and other mycobacterial diseases

2023 Volume 32, Page(s) 100377

Abstract: Background: Tuberculosis is a global public health problem. Extra-pulmonary tuberculosis accounts for an increasing proportion of cases worldwide, although information about epidemiological, clinical, or microbiological factors is lacking.: Methods: ... ...

Abstract	Background: Tuberculosis is a global public health problem. Extra-pulmonary tuberculosis accounts for an increasing proportion of cases worldwide, although information about epidemiological, clinical, or microbiological factors is lacking. Methods: We conducted a retrospective observational study of tuberculosis cases diagnosed between 2016 and 2021, classified into Pulmonary and Extra-pulmonary tuberculosis. Univariable and multivariable logistic regression models were used to investigate risk factors of Extra-pulmonary tuberculosis. Results: 20.9% of overall cases were classified as Extra-pulmonary tuberculosis, with a rising trend from 22.6% in 2016 to 27.9% in 2021. Lymphatic tuberculosis accounted for 50.6% of cases, followed by pleural tuberculosis (24.1%). 55.4% of cases belonged to foreign-born patients. Microbiological culture tested positive in 92.8% of Extra-pulmonary cases. Logistic regression analysis showed that women were more predisposed to develop Extra-pulmonary tuberculosis (aOR 2.46, 95% CI 1.45-4.20) as well as elderly patients (aged ≥ 65 years) (aOR 2.47, 95% CI 1.19-5.13) and persons with previous history of tuberculosis (4.99, 95% CI 1.40-17.82). Conclusions: Extra-pulmonary Tuberculosis have increased within our study period. A profound decline occurred in 2021 tuberculosis cases, probably due to COVID-19. Women, elderly population, and persons with previous history of tuberculosis are at higher risk of developing Extra-pulmonary tuberculosis in our setting.
Language	English
Publishing date	2023-05-12
Publishing country	England
Document type	Journal Article
ISSN	2405-5794
ISSN (online)	2405-5794
DOI	10.1016/j.jctube.2023.100377
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Article ; Online: Xpert MTB/RIF Ultra CT value provides a rapid measure of sputum bacillary burden and predicts smear status in patients with pulmonary tuberculosis.

Martin-Higuera, M C / Rivas, G / Rolo, M / Muñoz-Gallego, I / Lopez-Roa, Paula

Scientific reports

2023 Volume 13, Issue 1, Page(s) 1591

Abstract: Traditionally, smear microscopy has been used to estimate bacillary burden in order to assess infectiousness in tuberculosis (TB) patients. Since Xpert MTB assays might replace smear microscopy as the first-line diagnostic test for pulmonary tuberculosis, ...

Abstract	Traditionally, smear microscopy has been used to estimate bacillary burden in order to assess infectiousness in tuberculosis (TB) patients. Since Xpert MTB assays might replace smear microscopy as the first-line diagnostic test for pulmonary tuberculosis, an alternative measure of bacillary load that correlates with smear positivity is needed. This study assessed the correlation between C
MeSH term(s)	Humans ; Mycobacterium tuberculosis/genetics ; Bacillus ; Sputum ; Sensitivity and Specificity ; Tuberculosis, Pulmonary/diagnostic imaging ; Firmicutes ; Lacticaseibacillus casei ; Tomography, X-Ray Computed
Language	English
Publishing date	2023-01-28
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-28869-6
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Article ; Online: Unusual microscopic finding in a hepatic abscess content.

Rolo, Marta / Reyes, Alhena / Torres, Juan Martín / Pérez-Ayala, Ana

Enfermedades infecciosas y microbiologia clinica (English ed.)

2023 Volume 41, Issue 4, Page(s) 254–255

MeSH term(s)	Humans ; Liver Abscess ; Tomography, X-Ray Computed
Language	English
Publishing date	2023-01-25
Publishing country	Spain
Document type	Case Reports ; Journal Article
ISSN	2529-993X
ISSN (online)	2529-993X
DOI	10.1016/j.eimce.2022.06.018
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Article ; Online: Preliminary Characterization of an Active CMOS Pad Detector for Tracking and Dosimetry in HDR Brachytherapy.

Bui, Thi Ngoc Hang / Large, Matthew / Poder, Joel / Bucci, Joseph / Bianco, Edoardo / Giampaolo, Raffaele Aaron / Rivetti, Angelo / Da Rocha Rolo, Manuel / Pastuovic, Zeljko / Corradino, Thomas / Pancheri, Lucio / Petasecca, Marco

Sensors (Basel, Switzerland)

2024 Volume 24, Issue 2

Abstract: We assessed the accuracy of a prototype radiation detector with a built in CMOS amplifier for use in dosimetry for high dose rate brachytherapy. The detectors were fabricated on two substrates of epitaxial high resistivity silicon. The radiation ... ...

Abstract	We assessed the accuracy of a prototype radiation detector with a built in CMOS amplifier for use in dosimetry for high dose rate brachytherapy. The detectors were fabricated on two substrates of epitaxial high resistivity silicon. The radiation detection performance of prototypes has been tested by ion beam induced charge (IBIC) microscopy using a 5.5 MeV alpha particle microbeam. We also carried out the HDR Ir-192 radiation source tracking at different depths and angular dose dependence in a water equivalent phantom. The detectors show sensitivities spanning from (5.8 ± 0.021) × 10
Language	English
Publishing date	2024-01-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s24020692
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Article ; Online: Superior neutralizing response after first versus second SARS-CoV-2 infection in fully vaccinated individuals.

Rivas, Gonzalo / Labiod, Nuria / Luczkowiak, Joanna / Lasala, Fátima / Rolo, Marta / Mancheño-Losa, Mikel / Rial-Crestelo, David / Lora-Tamayo, Jaime / Pérez-Rivilla, Alfredo / Folgueira, María Dolores / Delgado, Rafael

Journal of medical virology

2023 Volume 95, Issue 11, Page(s) e29225

Abstract: Currently, the majority of the population has been vaccinated against COVID-19 and/or has experienced SARS-CoV-2 infection either before or after vaccination. The immunological response to repeated episodes of infections is not completely clear. We ... ...

Abstract	Currently, the majority of the population has been vaccinated against COVID-19 and/or has experienced SARS-CoV-2 infection either before or after vaccination. The immunological response to repeated episodes of infections is not completely clear. We measured SARS-CoV-2 specific neutralization titers by a pseudovirus assay after BA.1 infection and RBD-specific immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) in a cohort of COVID-19 uninfected and triple vaccinated individuals (breakthrough infection group, BTI) as compared with those previously infected by SARS-CoV-2 (reinfection group, REI) who underwent identical vaccination schedule. SARS-CoV-2 specific neutralizing response after BA.1 infection was significantly higher in the BTI group as compared with the REI. Furthermore, neutralization titers in REI were not significant different from convalescent non reinfected controls. RBD-specific IgG and IgA, but not IgM, were also significantly higher in BTI as compared with REI. Our results show that the first episode of SARS-CoV-2 infection induces a significant increase in neutralizing titers in triple vaccinated individuals and that previous SARS-CoV-2 infection compromise significantly the neutralization response induced by reinfection, even by divergent SARS-CoV-2 variants and at least up to 2 years postinfection, suggesting a fundamental limitation in inducing effective booster through the intranasal route in previously infected individuals.
MeSH term(s)	Humans ; COVID-19/prevention & control ; SARS-CoV-2 ; Reinfection ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Vaccination ; Antibodies, Neutralizing ; Antibodies, Viral
Chemical Substances	Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Antibodies, Neutralizing ; Antibodies, Viral
Language	English
Publishing date	2023-11-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.29225
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Article ; Online: Clinical impact of time-to-positivity of blood cultures on mortality in patients with Pseudomonas aeruginosa bacteremia.

Rolo, Marta / Martín-Higuera, María Carmen / Viedma, Esther / Villa, Jennifer / Mancheño-Losa, Mikel / Lora-Tamayo, Jaime / Chaves, Fernando / Orellana, María Ángeles / Recio, Raúl

Journal of global antimicrobial resistance

2022 Volume 30, Page(s) 269–275

Abstract: Objectives: To investigate the impact of the time-to-positivity of blood cultures (TTP) on 30-day mortality in patients with Pseudomonas aeruginosa bacteremia.: Methods: All nonduplicated episodes of P. aeruginosa monomicrobial bacteremia in adult ... ...

Abstract	Objectives: To investigate the impact of the time-to-positivity of blood cultures (TTP) on 30-day mortality in patients with Pseudomonas aeruginosa bacteremia. Methods: All nonduplicated episodes of P. aeruginosa monomicrobial bacteremia in adult patients from January 2013 to February 2020 were analysed. Epidemiological and clinical data were collected. TTP of blood cultures for P. aeruginosa isolates was automatically recorded. Multivariate analysis identified factors predicting 30-day overall mortality. Results: A total of 328 patients were identified. The median TTP for P. aeruginosa isolates was 15 h (interquartile range [IQR] 12-18 h). All multidrug-resistant and extensively drug-resistant (MDR/XDR) episodes were positive within the first 36 h. The 30-day mortality rate was 32.3%. The best cut-off value of the TTP for predicting mortality was 16 h (area under the receiver operating characteristic curve 0.62, 95% confidence interval [CI] 0.56-0.67, P = 0.001). The 30-day mortality rate was significantly higher in the TTP ≤16 h group (41.0% vs. 19.5%, P < 0.001). In a multivariate analysis, severe neutropenia (adjusted odds ratio [aOR] 2.67, 95% CI 1.4-5.09, P = 0.002), septic shock (aOR 3.21, 95% CI 1.57-5.89, P < 0.001), respiratory source (aOR 4.37, 95% CI 2.24-8.52, P < 0.001), nosocomial acquisition (aOR 1.99, 95% CI 1.06-3.71, P = 0.030), TTP ≤16 h (aOR 2.27, 95% CI 2.12-4.25, P = 0.010), and MDR/XDR phenotype (aOR 2.54, 95% CI 1.38-4.67, P = 0.002) were independently associated with 30-day mortality. Conclusions: A short TTP (≤16 h) was independently associated with increased 30-day mortality. After local validation, this routinely available microbiological parameter might be useful for guiding empirical antipseudomonal therapies and supporting the close monitoring of patients with P. aeruginosa bacteremia.
MeSH term(s)	Bacteremia/microbiology ; Blood Culture ; Humans ; Pseudomonas aeruginosa ; Risk Factors ; Shock, Septic
Language	English
Publishing date	2022-07-03
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2710046-7
ISSN	2213-7173 ; 2213-7173
ISSN (online)	2213-7173
ISSN	2213-7173
DOI	10.1016/j.jgar.2022.06.026
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Article ; Online: Cross neutralization of SARS-CoV-2 omicron subvariants after repeated doses of COVID-19 mRNA vaccines.

Luczkowiak, Joanna / Rivas, Gonzalo / Labiod, Nuria / Lasala, Fátima / Rolo, Marta / Lora-Tamayo, Jaime / Mancheno-Losa, Mikel / Rial-Crestelo, David / Pérez-Rivilla, Alfredo / Folgueira, María Dolores / Delgado, Rafael

Journal of medical virology

2022 Volume 95, Issue 1, Page(s) e28268

Abstract: We have measured the humoral response to messenger RNA (mRNA) vaccines in COVID-19 naïve and convalescent individuals. Third doses of mRNA COVID-19 vaccines induced a significant increase in potency and breadth of neutralization against SARS-CoV-2 ... ...

Abstract	We have measured the humoral response to messenger RNA (mRNA) vaccines in COVID-19 naïve and convalescent individuals. Third doses of mRNA COVID-19 vaccines induced a significant increase in potency and breadth of neutralization against SARS-CoV-2 variants of concern (VoC) including Omicron subvariants BA.1, BA.2, and BA.2.12.1, that were cross-neutralized at comparable levels and less for BA.4/5. This booster effect was especially important in naïve individuals that only after the third dose achieved a level that was comparable with that of vaccinated COVID-19 convalescents except for BA.4/5. Avidity of RBD-binding antibodies was also significantly increased in naïve individuals after the third dose, indicating an association between affinity maturation and cross neutralization of VoC. These results suggest that at least three antigenic stimuli by infection or vaccination with ancestral SARS-CoV-2 sequences are required to induce high avidity cross-neutralizing antibodies. Nevertheless, the circulation of new subvariants such as BA.4/5 with partial resistance to neutralization will have to be closely monitored and eventually consider for future vaccine developments.
MeSH term(s)	Humans ; COVID-19/prevention & control ; COVID-19 Vaccines ; SARS-CoV-2/genetics ; RNA, Messenger/genetics ; mRNA Vaccines ; Antibodies, Neutralizing ; Antibodies, Viral ; Spike Glycoprotein, Coronavirus
Chemical Substances	COVID-19 Vaccines ; RNA, Messenger ; mRNA Vaccines ; Antibodies, Neutralizing ; Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2022-11-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.28268
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Article: Prime-Boost Vaccination With BNT162b2 Induces High Neutralizing Activity Against SARS-CoV-2 Variants in Naïve and COVID-19-Convalescent Individuals.

Luczkowiak, Joanna / Labiod, Nuria / Rivas, Gonzalo / Rolo, Marta / Lasala, Fátima / Lora-Tamayo, Jaime / Mancheno-Losa, Mikel / Rial, David / Pérez-Rivilla, Alfredo / Folgueira, María D / Delgado, Rafael

Open forum infectious diseases

2021 Volume 8, Issue 10, Page(s) ofab468

Abstract: Background: The objective of this study was to investigate the neutralizing response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VoC) during coronavirus disease 2019 (COVID-19) convalescence and after ... ...

Abstract	Background: The objective of this study was to investigate the neutralizing response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VoC) during coronavirus disease 2019 (COVID-19) convalescence and after vaccination. Methods: COVID-19-convalescent and -naïve individuals were tested for neutralizing activity against SARS-CoV-2 VoC Alpha, Beta, Gamma, and Delta at 1 and 7 months postinfection and 4-6 weeks after BNT162b2 vaccination. Results: Vaccination induced a high neutralizing response in naïve individuals. Interestingly, vaccination of convalescent patients induced a boosted response that was able to neutralize all VoC at high titers. Conclusions: Vaccination with BNT162b2 induced high levels of neutralization against SARS-CoV-2 VoC in most patients; this is especially beneficial in COVID-19-convalescent individuals.
Language	English
Publishing date	2021-09-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	2757767-3
ISSN	2328-8957
ISSN	2328-8957
DOI	10.1093/ofid/ofab468
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Article ; Online: Neutralizing Response Against SARS-CoV-2 Variants 8 Months After BNT162b2 Vaccination in Naive and COVID-19-Convalescent Individuals.

Luczkowiak, Joanna / Labiod, Nuria / Rivas, Gonzalo / Rolo, Marta / Lasala, Fátima / Lora-Tamayo, Jaime / Mancheno-Losa, Mikel / Rial-Crestelo, David / Pérez-Rivilla, Alfredo / Folgueira, María Dolores / Delgado, Rafael

The Journal of infectious diseases

2021 Volume 225, Issue 11, Page(s) 1905–1908

Abstract: We have investigated the evolution of the neutralizing response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants at 8 months after Pfizer-BNT162b2 vaccination in coronavirus disease 2019 (COVID-19)-naive (n = 21) and COVID-19- ...

Abstract	We have investigated the evolution of the neutralizing response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants at 8 months after Pfizer-BNT162b2 vaccination in coronavirus disease 2019 (COVID-19)-naive (n = 21) and COVID-19-convalescent (n = 21) individuals. Neutralizing levels declined for all variants (range 2- to 3.7-fold). Eight months after vaccination, a significant proportion (4/21) of naive individuals lacked detectable neutralizing activity against the highly transmissible SARS-CoV-2 delta variant. In the convalescent group, the impressive high initial humoral response resulted in detectable neutralizing antibody levels against all variants throughout this period.
MeSH term(s)	Antibodies, Neutralizing ; Antibodies, Viral ; BNT162 Vaccine ; COVID-19/prevention & control ; Humans ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus ; Vaccination
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; BNT162 Vaccine (N38TVC63NU)
Language	English
Publishing date	2021-12-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3019-3
ISSN	1537-6613 ; 0022-1899
ISSN (online)	1537-6613
ISSN	0022-1899
DOI	10.1093/infdis/jiab634
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Uh II Zs.50: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MO 445: Show issues

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Article ; Online: Intranasal delivery of a synthetic Entamoeba histolytica vaccine containing adjuvant (LecA + GLA-3 M-052 liposomes): In vitro characterization.

Murphy, Brynn M / Chen, John Z / Rolo, Michael / Eldam, Mohamed / Jordan, Lynn / Sivananthan, Sandra J / Kinsey, Robert / Guderian, Jeffrey A / Pedersen, Karl / Abhyankar, Mayuresh / Petri, William A / Fox, Christopher B / Finlay, Warren H / Vehring, Reinhard / Martin, Andrew R

International journal of pharmaceutics

2022 Volume 626, Page(s) 122141

Abstract: Amebiasis, a disease caused by the parasite Entamoeba histolytica, is estimated to cause millions of infections and at least 55,000 deaths globally each year. With no vaccine currently available, there is an urgent need for an accessible means of ... ...

Abstract	Amebiasis, a disease caused by the parasite Entamoeba histolytica, is estimated to cause millions of infections and at least 55,000 deaths globally each year. With no vaccine currently available, there is an urgent need for an accessible means of stimulating protective mucosal immunity. The objective of this study was to characterize the nasal spray of a novel amebiasis vaccine candidate from a syringe-based liquid atomization device, the Teleflex MAD Nasal™, in both adult and infant nasal airways. Human ergonomic testing was completed to determine realistic actuation parameters. Spray pattern, plume geometry, and droplet size distribution were measured to evaluate reproducibility of free plume characteristics. The Alberta Idealized Nasal Inlet (AINI) and three realistic infant nasal airways were used to determine the in vitro deposition profile in adult and infant airways, respectively. Collectively, in vitro results demonstrated the feasibility of delivering the vaccine candidate to target sites within the nasal airways. Penetration through the nasal airways that could lead to deposition in the lungs was below the limit of quantification for both adult and infant geometries, indicating a low likelihood of adverse events due to lung exposure. These results support continued investigation of intranasal delivery of the synthetic Entamoeba histolytica vaccine.
MeSH term(s)	Adjuvants, Pharmaceutic ; Adjuvants, Vaccine ; Administration, Intranasal ; Adult ; Aerosols ; Amebiasis ; Entamoeba histolytica ; Humans ; Liposomes ; Nasal Sprays ; Reproducibility of Results ; Vaccines, Synthetic
Chemical Substances	Adjuvants, Pharmaceutic ; Adjuvants, Vaccine ; Aerosols ; Liposomes ; Nasal Sprays ; Vaccines, Synthetic
Language	English
Publishing date	2022-09-02
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	428962-6
ISSN	1873-3476 ; 0378-5173
ISSN (online)	1873-3476
ISSN	0378-5173
DOI	10.1016/j.ijpharm.2022.122141
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