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Article ; Online: Salivary IgA subtypes as novel disease biomarkers in systemic lupus erythematosus.

Romero-Ramírez, Sandra / Sosa-Hernández, Víctor A / Cervantes-Díaz, Rodrigo / Carrillo-Vázquez, Daniel A / Meza-Sánchez, David E / Núñez-Álvarez, Carlos / Torres-Ruiz, Jiram / Gómez-Martín, Diana / Maravillas-Montero, José L

Frontiers in immunology

2023 Volume 14, Page(s) 1080154

Abstract: Introduction: Immunoglobulin A (IgA) is the main antibody isotype in body fluids such as tears, intestinal mucous, colostrum, and saliva. There are two subtypes of IgA in humans: IgA1, mainly present in blood and mucosal sites, and IgA2, preferentially ... ...

Abstract	Introduction: Immunoglobulin A (IgA) is the main antibody isotype in body fluids such as tears, intestinal mucous, colostrum, and saliva. There are two subtypes of IgA in humans: IgA1, mainly present in blood and mucosal sites, and IgA2, preferentially expressed in mucosal sites like the colon. In clinical practice, immunoglobulins are typically measured in venous or capillary blood; however, alternative samples, including saliva, are now being considered, given their non-invasive and easy collection nature. Several autoimmune diseases have been related to diverse abnormalities in oral mucosal immunity, such as rheumatoid arthritis, Sjogren's syndrome, and systemic lupus erythematosus (SLE). Methods: We decided to evaluate the levels of both IgA subtypes in the saliva of SLE patients. A light chain capture-based ELISA measured specific IgA1 and IgA2 levels in a cohort of SLE patients compared with age and gender-matched healthy volunteers. Results: Surprisingly, our results indicated that in the saliva of SLE patients, total IgA and IgA1 subtype were significantly elevated; we also found that salivary IgA levels, particularly IgA2, positively correlate with anti-dsDNA IgG antibody titers. Strikingly, we also detected the presence of salivary anti-nucleosome IgA antibodies in SLE patients, a feature not previously reported elsewhere. Conclusions: According to our results and upon necessary validation, IgA characterization in saliva could represent a potentially helpful tool in the clinical care of SLE patients with the advantage of being a more straightforward, faster, and safer method than manipulating blood samples.
MeSH term(s)	Humans ; Immunoglobulin A, Secretory ; Lupus Erythematosus, Systemic ; Immunoglobulin A ; Immunoglobulin G ; Mouth Mucosa ; Biomarkers
Chemical Substances	Immunoglobulin A, Secretory ; Immunoglobulin A ; Immunoglobulin G ; Biomarkers
Language	English
Publishing date	2023-02-22
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1080154
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Article ; Online: Circulating B10 regulatory cells are decreased in severe and critical COVID-19.

Cervantes-Díaz, Rodrigo / Sosa-Hernández, Víctor A / Romero-Ramírez, Sandra / Torres-Ruiz, Jiram / Pérez-Fragoso, Alfredo / Meza-Sánchez, David E / Gómez-Martín, Diana / Maravillas-Montero, José L

Journal of leukocyte biology

2022 Volume 112, Issue 2, Page(s) 333–337

Abstract: The contribution of B cells in COVID-19 pathogenesis, beyond the production of specific antibodies against SARS-CoV-2, is still not well understood. Since one of their most relevant functional roles includes their immune-suppressive mechanisms, we ... ...

Abstract	The contribution of B cells in COVID-19 pathogenesis, beyond the production of specific antibodies against SARS-CoV-2, is still not well understood. Since one of their most relevant functional roles includes their immune-suppressive mechanisms, we decided to evaluate one of the most recognized human B regulatory subpopulations: the IL-10
MeSH term(s)	B-Lymphocytes, Regulatory ; COVID-19 ; Flow Cytometry ; Humans ; Interleukin-10 ; SARS-CoV-2
Chemical Substances	Interleukin-10 (130068-27-8)
Language	English
Publishing date	2022-02-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605722-6
ISSN	1938-3673 ; 0741-5400
ISSN (online)	1938-3673
ISSN	0741-5400
DOI	10.1002/JLB.5COVCRA0721-387RR
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Article ; Online: Severity of SARS-CoV-2 infection is linked to double-negative (CD27

Cervantes-Díaz, Rodrigo / Sosa-Hernández, Víctor Andrés / Torres-Ruíz, Jiram / Romero-Ramírez, Sandra / Cañez-Hernández, Mariana / Pérez-Fragoso, Alfredo / Páez-Franco, José C / Meza-Sánchez, David E / Pescador-Rojas, Miriam / Sosa-Hernández, Víctor Adrián / Gómez-Martín, Diana / Maravillas-Montero, José L

Inflammation research : official journal of the European Histamine Research Society ... [et al.

2021 Volume 71, Issue 1, Page(s) 131–140

Abstract: Objectives: The role of B cells in COVID-19, beyond the production of specific antibodies against SARS-CoV-2, is still not well understood. Here, we describe the novel landscape of circulating double-negative (DN) CD27: Methods: Using multiparametric ...

Abstract	Objectives: The role of B cells in COVID-19, beyond the production of specific antibodies against SARS-CoV-2, is still not well understood. Here, we describe the novel landscape of circulating double-negative (DN) CD27 Methods: Using multiparametric flow cytometry, we determined DN B cell subset amounts from 91 COVID-19 patients, correlated those with cytokines, clinical and laboratory parameters, and segregated them by principal components analysis. Results: We detected significant increments in the DN2 and DN3 B cell subsets, while we found a relevant decrease in the DN1 B cell subpopulation, according to disease severity and patient outcomes. These DN cell numbers also appeared to correlate with pro- or anti-inflammatory signatures, respectively, and contributed to the segregation of the patients into disease severity groups. Conclusion: This study provides insights into DN B cell subsets' potential role in immune responses against SARS-CoV-2, particularly linked to the severity of COVID-19.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; B-Lymphocytes/cytology ; COVID-19/blood ; COVID-19/diagnosis ; COVID-19/immunology ; COVID-19/virology ; Cell Lineage ; Computational Biology ; Disease Progression ; Female ; Humans ; Immunoglobulin D/blood ; Male ; Middle Aged ; Principal Component Analysis ; Prognosis ; Respiration, Artificial ; SARS-CoV-2 ; Severity of Illness Index ; Tumor Necrosis Factor Receptor Superfamily, Member 7/blood ; Young Adult
Chemical Substances	Immunoglobulin D ; Tumor Necrosis Factor Receptor Superfamily, Member 7
Language	English
Publishing date	2021-11-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1221794-3
ISSN	1420-908X ; 1023-3830
ISSN (online)	1420-908X
ISSN	1023-3830
DOI	10.1007/s00011-021-01525-3
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Article ; Online: CD11c

Sosa-Hernández, Víctor A / Romero-Ramírez, Sandra / Cervantes-Díaz, Rodrigo / Carrillo-Vázquez, Daniel A / Navarro-Hernandez, Itze C / Whittall-García, Laura P / Absalón-Aguilar, Abdiel / Vargas-Castro, Ana S / Reyes-Huerta, Raúl F / Juárez-Vega, Guillermo / Meza-Sánchez, David E / Ortiz-Navarrete, Vianney / Torres-Ruiz, Jiram / Mejía-Domínguez, Nancy R / Gómez-Martín, Diana / Maravillas-Montero, José L

Frontiers in immunology

2022 Volume 13, Page(s) 892241

Abstract: Lupus nephritis (LN) is one of the most common manifestations of systemic lupus erythematosus (SLE), characterized by abnormal B cell activation and differentiation to memory or plasma effector cells. However, the role of these cells in the pathogenesis ... ...

Abstract	Lupus nephritis (LN) is one of the most common manifestations of systemic lupus erythematosus (SLE), characterized by abnormal B cell activation and differentiation to memory or plasma effector cells. However, the role of these cells in the pathogenesis of LN is not fully understood, as well as the effect of induction therapy on B cell subsets, possibly associated with this manifestation, like aged-associated B cells (ABCs). Consequently, we analyzed the molecules defining the ABCs subpopulation (CD11c, T-bet, and CD21) through flow cytometry of blood samples from patients with lupus presenting or not LN, following up a small sub-cohort after six months of induction therapy. The frequency of ABCs resulted higher in LN patients compared to healthy subjects. Unexpectedly, we identified a robust reduction of a CD21
MeSH term(s)	Aged ; B-Lymphocyte Subsets ; Biomarkers ; CD11c Antigen ; Complement System Proteins/therapeutic use ; Humans ; Lupus Erythematosus, Systemic/complications ; Lupus Erythematosus, Systemic/diagnosis ; Lupus Erythematosus, Systemic/drug therapy ; Lupus Nephritis/diagnosis ; Renal Insufficiency
Chemical Substances	Biomarkers ; CD11c Antigen ; Complement System Proteins (9007-36-7)
Language	English
Publishing date	2022-05-19
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.892241
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Article ; Online: Myosin 1F Regulates M1-Polarization by Stimulating Intercellular Adhesion in Macrophages.

Piedra-Quintero, Zayda L / Serrano, Carolina / Villegas-Sepúlveda, Nicolás / Maravillas-Montero, José L / Romero-Ramírez, Sandra / Shibayama, Mineko / Medina-Contreras, Oscar / Nava, Porfirio / Santos-Argumedo, Leopoldo

Frontiers in immunology

2019 Volume 9, Page(s) 3118

Abstract: Intestinal macrophages are highly mobile cells with extraordinary plasticity and actively contribute to cytokine-mediated epithelial cell damage. The mechanisms triggering macrophage polarization into a proinflammatory phenotype are unknown. Here, we ... ...

Abstract	Intestinal macrophages are highly mobile cells with extraordinary plasticity and actively contribute to cytokine-mediated epithelial cell damage. The mechanisms triggering macrophage polarization into a proinflammatory phenotype are unknown. Here, we report that during inflammation macrophages enhance its intercellular adhesion properties in order to acquire a M1-phenotype. Using
MeSH term(s)	Animals ; Cell Line, Tumor ; Colitis, Ulcerative/chemically induced ; Colitis, Ulcerative/immunology ; Cytoskeleton/immunology ; Cytoskeleton/metabolism ; Dextran Sulfate/administration & dosage ; Dextran Sulfate/toxicity ; Disease Models, Animal ; Humans ; Integrin alphaVbeta3/immunology ; Integrin alphaVbeta3/metabolism ; Interleukin-1beta/immunology ; Interleukin-1beta/metabolism ; Macrophage Activation ; Macrophages/immunology ; Macrophages/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Myosin Type I/genetics ; Myosin Type I/immunology ; Myosin Type I/metabolism ; Primary Cell Culture ; RAW 264.7 Cells
Chemical Substances	IL1B protein, mouse ; Integrin alphaVbeta3 ; Interleukin-1beta ; Myo1f protein, mouse ; Dextran Sulfate (9042-14-2) ; Myosin Type I (EC 3.6.1.-)
Language	English
Publishing date	2019-01-10
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2018.03118
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Article: Participación de los linfocitos B reguladores (Breg) en las enfermedades alérgicas.

Navarro-Hernández, Itze Cecilia / Cervantes-Díaz, Rodrigo / Romero-Ramírez, Sandra / Sosa-Hernández, Víctor Andrés / Kleinberg, Ari / Meza-Sánchez, David Eduardo / Maravillas-Montero, José L

Revista alergia Mexico (Tecamachalco, Puebla, Mexico : 1993)

2019 Volume 65, Issue 4, Page(s) 400–413

Abstract: Immune tolerance, both to exogenous antigens and autoantigens, is essential for restraining undesired inflammatory responses that might result in severe damage to body tissues or cause chronic diseases. During the past few decades, different cell ... ...

Title translation	Regulatory B cells (Bregs) role in allergic diseases.
Abstract	Immune tolerance, both to exogenous antigens and autoantigens, is essential for restraining undesired inflammatory responses that might result in severe damage to body tissues or cause chronic diseases. During the past few decades, different cell populations and molecules by them secreted have been associated with suppressing and regulatory mechanisms of immune responses. Although B cells typically acquire relevance as precursors of antibody-producing cells, they can also develop potent regulatory functions through the production of soluble molecules or by establishing direct cellular interactions mediated by different surface proteins implicated in signal transduction. While most studies of regulatory B cells define the role of these lymphocytes in autoimmune diseases, evidence of their importance and mechanisms of action in allergic diseases has accumulated in recent years. As a result, regulatory B cells appear to be relevant elements for the establishment or loss of allergen tolerance in different allergic diseases, although they still have been little explored.
MeSH term(s)	Animals ; B-Lymphocytes, Regulatory/physiology ; Disease Models, Animal ; Humans ; Hypersensitivity/immunology ; Immune Tolerance
Language	Spanish
Publishing date	2019-01-02
Publishing country	Mexico
Document type	Journal Article
ZDB-ID	639125-4
ISSN	0002-5151
ISSN	0002-5151
DOI	10.29262/ram.v65i4.529
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Article ; Online: Redefining COVID-19 Severity and Prognosis: The Role of Clinical and Immunobiotypes.

Torres-Ruiz, Jiram / Pérez-Fragoso, Alfredo / Maravillas-Montero, José Luis / Llorente, Luis / Mejía-Domínguez, Nancy R / Páez-Franco, José Carlos / Romero-Ramírez, Sandra / Sosa-Hernández, Victor Andrés / Cervantes-Díaz, Rodrigo / Absalón-Aguilar, Abdiel / Nuñez-Aguirre, Miroslava / Juárez-Vega, Guillermo / Meza-Sánchez, David / Kleinberg-Bid, Ari / Hernández-Gilsoul, Thierry / Ponce-de-León, Alfredo / Gómez-Martín, Diana

Frontiers in immunology

2021 Volume 12, Page(s) 689966

Abstract: Background: Most of the explanatory and prognostic models of COVID-19 lack of a comprehensive assessment of the wide COVID-19 spectrum of abnormalities. The aim of this study was to unveil novel biological features to explain COVID-19 severity and ... ...

Abstract	Background: Most of the explanatory and prognostic models of COVID-19 lack of a comprehensive assessment of the wide COVID-19 spectrum of abnormalities. The aim of this study was to unveil novel biological features to explain COVID-19 severity and prognosis (death and disease progression). Methods: A predictive model for COVID-19 severity in 121 patients was constructed by ordinal logistic regression calculating odds ratio (OR) with 95% confidence intervals (95% CI) for a set of clinical, immunological, metabolomic, and other biological traits. The accuracy and calibration of the model was tested with the area under the curve (AUC), Somer's D, and calibration plot. Hazard ratios with 95% CI for adverse outcomes were calculated with a Cox proportional-hazards model. Results: The explanatory variables for COVID-19 severity were the body mass index (BMI), hemoglobin, albumin, 3-Hydroxyisovaleric acid, CD8+ effector memory T cells, Th1 cells, low-density granulocytes, monocyte chemoattractant protein-1, plasma TRIM63, and circulating neutrophil extracellular traps. The model showed an outstanding performance with an optimism-adjusted AUC of 0.999, and Somer's D of 0.999. The predictive variables for adverse outcomes in COVID-19 were severe and critical disease diagnosis, BMI, lactate dehydrogenase, Troponin I, neutrophil/lymphocyte ratio, serum levels of IP-10, malic acid, 3, 4 di-hydroxybutanoic acid, citric acid, myoinositol, and cystine. Conclusions: Herein, we unveil novel immunological and metabolomic features associated with COVID-19 severity and prognosis. Our models encompass the interplay among innate and adaptive immunity, inflammation-induced muscle atrophy and hypoxia as the main drivers of COVID-19 severity.
MeSH term(s)	Adult ; Blood Coagulation ; Body Mass Index ; COVID-19/blood ; COVID-19/immunology ; COVID-19/metabolism ; Cytokines/blood ; Extracellular Traps/immunology ; Female ; Hemoglobins/analysis ; Humans ; Male ; Metabolome ; Middle Aged ; Muscular Atrophy ; Neutrophils/immunology ; Phenotype ; Prognosis ; SARS-CoV-2 ; Serum Albumin, Human/analysis ; Severity of Illness Index ; T-Lymphocytes/immunology ; Valerates/blood
Chemical Substances	Cytokines ; Hemoglobins ; Valerates ; beta-hydroxyisovaleric acid (3F752311CD) ; Serum Albumin, Human (ZIF514RVZR)
Language	English
Publishing date	2021-09-08
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2021.689966
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Article ; Online: Metabolomics analysis reveals a modified amino acid metabolism that correlates with altered oxygen homeostasis in COVID-19 patients.

Páez-Franco, José C / Torres-Ruiz, Jiram / Sosa-Hernández, Víctor A / Cervantes-Díaz, Rodrigo / Romero-Ramírez, Sandra / Pérez-Fragoso, Alfredo / Meza-Sánchez, David E / Germán-Acacio, Juan Manuel / Maravillas-Montero, José L / Mejía-Domínguez, Nancy R / Ponce-de-León, Alfredo / Ulloa-Aguirre, Alfredo / Gómez-Martín, Diana / Llorente, Luis

Scientific reports

2021 Volume 11, Issue 1, Page(s) 6350

Abstract: We identified the main changes in serum metabolites associated with severe (n = 46) and mild (n = 19) COVID-19 patients by gas chromatography coupled to mass spectrometry. The modified metabolic profiles were associated to an altered amino acid ... ...

Abstract	We identified the main changes in serum metabolites associated with severe (n = 46) and mild (n = 19) COVID-19 patients by gas chromatography coupled to mass spectrometry. The modified metabolic profiles were associated to an altered amino acid catabolism in hypoxic conditions. Noteworthy, three α-hydroxyl acids of amino acid origin increased with disease severity and correlated with altered oxygen saturation levels and clinical markers of lung damage. We hypothesize that the enzymatic conversion of α-keto-acids to α- hydroxyl-acids helps to maintain NAD recycling in patients with altered oxygen levels, highlighting the potential relevance of amino acid supplementation during SARS-CoV-2 infection.
MeSH term(s)	Adult ; Amino Acids/metabolism ; COVID-19/metabolism ; Case-Control Studies ; Female ; Homeostasis ; Humans ; Male ; Metabolomics ; Middle Aged ; Mitochondria/metabolism ; Oxygen/metabolism
Chemical Substances	Amino Acids ; Oxygen (S88TT14065)
Language	English
Publishing date	2021-03-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-85788-0
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Article ; Online: Severe COVID-19 is marked by dysregulated serum levels of carboxypeptidase A3 and serotonin.

Soria-Castro, Rodolfo / Meneses-Preza, Yatsiri G / Rodríguez-López, Gloria M / Romero-Ramírez, Sandra / Sosa-Hernández, Víctor A / Cervantes-Díaz, Rodrigo / Pérez-Fragoso, Alfredo / Torres-Ruíz, José J / Gómez-Martín, Diana / Campillo-Navarro, Marcia / Álvarez-Jiménez, Violeta D / Pérez-Tapia, Sonia M / Chávez-Blanco, Alma D / Estrada-Parra, Sergio / Maravillas-Montero, José L / Chacón-Salinas, Rommel

Journal of leukocyte biology

2021 Volume 110, Issue 3, Page(s) 425–431

Abstract: The immune response plays a critical role in the pathophysiology of SARS-CoV-2 infection ranging from protection to tissue damage and all occur in the development of acute respiratory distress syndrome (ARDS). ARDS patients display elevated levels of ... ...

Abstract	The immune response plays a critical role in the pathophysiology of SARS-CoV-2 infection ranging from protection to tissue damage and all occur in the development of acute respiratory distress syndrome (ARDS). ARDS patients display elevated levels of inflammatory cytokines and innate immune cells, and T and B cell lymphocytes have been implicated in this dysregulated immune response. Mast cells are abundant resident cells of the respiratory tract and are able to release different inflammatory mediators rapidly following stimulation. Recently, mast cells have been associated with tissue damage during viral infections, but their role in SARS-CoV-2 infection remains unclear. In this study, we examined the profile of mast cell activation markers in the serum of COVID-19 patients. We noticed that SARS-CoV-2-infected patients showed increased carboxypeptidase A3 (CPA3) and decreased serotonin levels in their serum when compared with symptomatic SARS-CoV-2-negative patients. CPA3 levels correlated with C-reactive protein, the number of circulating neutrophils, and quick SOFA. CPA3 in serum was a good biomarker for identifying severe COVID-19 patients, whereas serotonin was a good predictor of SARS-CoV-2 infection. In summary, our results show that serum CPA3 and serotonin levels are relevant biomarkers during SARS-CoV-2 infection. This suggests that mast cells and basophils are relevant players in the inflammatory response in COVID-19 and may represent targets for therapeutic intervention.
MeSH term(s)	Biomarkers/analysis ; COVID-19/complications ; COVID-19/diagnosis ; COVID-19/metabolism ; COVID-19/virology ; Carboxypeptidases A/metabolism ; Humans ; Inflammation/diagnosis ; Inflammation/etiology ; Inflammation/metabolism ; Inflammation/pathology ; Inflammation Mediators/metabolism ; Mast Cells/immunology ; Mast Cells/pathology ; SARS-CoV-2/isolation & purification ; Serotonin/metabolism ; Severity of Illness Index
Chemical Substances	Biomarkers ; Inflammation Mediators ; Serotonin (333DO1RDJY) ; CPA3 protein, human (EC 3.4.17.1) ; Carboxypeptidases A (EC 3.4.17.1)
Language	English
Publishing date	2021-05-31
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605722-6
ISSN	1938-3673 ; 0741-5400
ISSN (online)	1938-3673
ISSN	0741-5400
DOI	10.1002/JLB.4HI0221-087R
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Article: Tetraspanin 33 (TSPAN33) regulates endocytosis and migration of human B lymphocytes by affecting the tension of the plasma membrane

Navarro‐Hernandez, Itze C / López‐Ortega, Orestes / Acevedo‐Ochoa, Ernesto / Cervantes‐Díaz, Rodrigo / Romero‐Ramírez, Sandra / Sosa‐Hernández, Víctor A / Meza‐Sánchez, David E / Juárez‐Vega, Guillermo / Pérez‐Martínez, César A / Chávez‐Munguía, Bibiana / Galván‐Hernández, Arturo / Antillón, Armando / Ortega‐Blake, Iván / Santos‐Argumedo, Leopoldo / Hernández‐Hernández, José M / Maravillas‐Montero, José L

FEBS journal. 2020 Aug., v. 287, no. 16

2020

Abstract: B lymphocytes are a leukocyte subset capable of developing several functions apart from differentiating into antibody‐secreting cells. These processes are triggered by external activation signals that induce changes in the plasma membrane properties, ... ...

Abstract	B lymphocytes are a leukocyte subset capable of developing several functions apart from differentiating into antibody‐secreting cells. These processes are triggered by external activation signals that induce changes in the plasma membrane properties, regulated by the formation of different lipid‐bilayer subdomains that are associated with the underlying cytoskeleton through different linker molecules, thus allowing the functional specialization of regions within the membrane. Among these, there are tetraspanin‐enriched domains. Tetraspanins constitute a superfamily of transmembrane proteins that establish lateral associations with other molecules, determining its activity and localization. In this study, we identified TSPAN33 as an active player during B‐lymphocyte cytoskeleton and plasma membrane‐related phenomena, including protrusion formation, adhesion, phagocytosis, and cell motility. By using an overexpression model of TSPAN33 in human Raji cells, we detected a specific distribution of this protein that includes membrane microvilli, the Golgi apparatus, and extracellular vesicles. Additionally, we identified diminished phagocytic ability and altered cell adhesion properties due to the aberrant expression of integrins. Accordingly, these cells presented an enhanced migratory phenotype, as shown by its augmented chemotaxis and invasion rates. When we evaluated the mechanic response of cells during fibronectin‐induced spreading, we found that TSPAN33 expression inhibited changes in roughness and membrane tension. Contrariwise, TSPAN33 knockdown cells displayed opposite phenotypes to those observed in the overexpression model. Altogether, our data indicate that TSPAN33 represents a regulatory element of the adhesion and migration of B lymphocytes, suggesting a novel implication of this tetraspanin in the control of the mechanical properties of their plasma membrane.
Keywords	B-lymphocytes ; Golgi apparatus ; adhesion ; cell adhesion ; cell movement ; chemotaxis ; cytoskeleton ; humans ; integrins ; microvilli ; migratory behavior ; models ; phagocytosis ; phenotype ; plasma membrane ; roughness
Language	English
Dates of publication	2020-08
Size	p. 3449-3471.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	NAL-AP-2-clean ; JOURNAL ARTICLE
ZDB-ID	2173655-8
ISSN	1742-4658 ; 1742-464X
ISSN (online)	1742-4658
ISSN	1742-464X
DOI	10.1111/febs.15216
Database	NAL-Catalogue (AGRICOLA)

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