Article ; Online: A high efficiency precision genome editing method with CRISPR in iPSCs.
2024 Volume 14, Issue 1, Page(s) 9933
Abstract: The use of genetic engineering to generate point mutations in induced pluripotent stem cells (iPSCs) is essential for studying a specific genetic effect in an isogenic background. We demonstrate that a combination of p53 inhibition and pro-survival small ...
| Abstract | The use of genetic engineering to generate point mutations in induced pluripotent stem cells (iPSCs) is essential for studying a specific genetic effect in an isogenic background. We demonstrate that a combination of p53 inhibition and pro-survival small molecules achieves a homologous recombination rate higher than 90% using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in human iPSCs. Our protocol reduces the effort and time required to create isogenic lines. |
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| MeSH term(s) | Induced Pluripotent Stem Cells/metabolism ; Induced Pluripotent Stem Cells/cytology ; Humans ; Gene Editing/methods ; CRISPR-Cas Systems ; Tumor Suppressor Protein p53/genetics ; Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; Homologous Recombination |
| Chemical Substances | Tumor Suppressor Protein p53 |
| Language | English |
| Publishing date | 2024-04-30 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2615211-3 |
| ISSN | 2045-2322 ; 2045-2322 |
| ISSN (online) | 2045-2322 |
| ISSN | 2045-2322 |
| DOI | 10.1038/s41598-024-60766-4 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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