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  1. Article ; Online: Spiritual and Emotional Experience With a Diagnosis of Breast Cancer: A Scoping Review.

    Leão, Diva Cristina M R / Pereira, Eliane R / Silva, Rose Mary C R A / Rocha, Renata Carla N P / Cruz-Quintana, Francisco / García-Caro, María Paz

    Cancer nursing

    2021  Volume 45, Issue 3, Page(s) 224–235

    Abstract: Background: The breast cancer diagnosis causes a high level of suffering and distress in patients who experience difficulties in coping. There is a need to improve knowledge of emotional and spiritual coping in response to the stressful situation of ... ...

    Abstract Background: The breast cancer diagnosis causes a high level of suffering and distress in patients who experience difficulties in coping. There is a need to improve knowledge of emotional and spiritual coping in response to the stressful situation of women who must face this diagnosis.
    Objectives: The aims of this study were to map women's spiritual and emotional coping experiences reported after a breast cancer diagnosis and examine the proposed interventions and suggestions for clinical practice.
    Methods: A scoping review was performed by searching the Scientific Electronic Library Online, Scopus, Cumulative Index to Nursing and Allied Health Literature, Latin American & Caribbean Health Sciences Literature, Medical Literature Analyses and Retrieval System Online, Spanish Bibliographic Index of Health Sciences, PSYCINFO, and Google Scholar databases using Medical Subject Headings terms. Additional pertinent studies were identified by reviewing the bibliographies of the included studies. Twenty articles were included according to the recommendations for scoping reviews.
    Results: Study findings regarding emotional and spiritual coping with the diagnosis and proposed interventions were synthesized. A thematic list of interventions and recommendations for clinical practice is also provided.
    Conclusions: The studies demonstrated that women with breast cancer are challenged by their emotions and experiences. The review highlights the importance of spiritual coping for redefining women's meaning in life. In clinical practice, caring for women's inherent needs when they are coping with a diagnosis is important to establish integral care.
    Implications for practice: Nurses can evaluate coping strategies, offer support for adaptation to the disease, provide qualified listening, help women in their search for significance while coping with cancer, and help them identify ways to overcome this stressful situation. Similarly, they can encourage patients to find spiritual comfort and emotional support.
    MeSH term(s) Adaptation, Psychological ; Breast Neoplasms/diagnosis ; Breast Neoplasms/psychology ; Emotions ; Female ; Humans ; Spirituality
    Language English
    Publishing date 2021-03-04
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 391995-x
    ISSN 1538-9804 ; 0162-220X
    ISSN (online) 1538-9804
    ISSN 0162-220X
    DOI 10.1097/NCC.0000000000000936
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    Zs.A 1538: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: A novel dissociative steroid VBP15 reduces MUC5AC gene expression in airway epithelial cells but lacks the GRE mediated transcriptional properties of dexamethasone.

    Garvin, Lindsay M / Chen, Yajun / Damsker, Jesse M / Rose, Mary C

    Pulmonary pharmacology & therapeutics

    2016  Volume 38, Page(s) 17–26

    Abstract: Overproduction of secretory mucins contributes to morbidity/mortality in inflammatory lung diseases. Inflammatory mediators directly increase expression of mucin genes, but few drugs have been shown to directly repress mucin gene expression. IL-1β ... ...

    Abstract Overproduction of secretory mucins contributes to morbidity/mortality in inflammatory lung diseases. Inflammatory mediators directly increase expression of mucin genes, but few drugs have been shown to directly repress mucin gene expression. IL-1β upregulates the MUC5AC mucin gene in part via the transcription factors NFκB while the glucocorticoid Dexamethasone (Dex) transcriptionally represses MUC5AC expression by Dex-activated GR binding to two GRE cis-sites in the MUC5AC promoter in lung epithelial cells. VBP compounds (ReveraGen BioPharma) maintain anti-inflammatory activity through inhibition of NFκB but exhibit reduced GRE-mediated transcriptional properties associated with adverse side-effects and thus have potential to minimize harmful side effects of long-term steroid therapy in inflammatory lung diseases. We investigated VBP15 efficacy as an anti-mucin agent in two types of airway epithelial cells and analyzed the transcription factor activity and promoter binding associated with VBP15-induced MUC5AC repression. VBP15 reduced MUC5AC mRNA abundance in a dose- and time-dependent manner similar to Dex in the presence or absence of IL-1β in A549 and differentiated human bronchial epithelial cells. Repression was abrogated in the presence of RU486, demonstrating a requirement for GR in the VBP15-induced repression of MUC5AC. Inhibition of NFκB activity resulted in reduced baseline expression of MUC5AC indicating that constitutive activity maintains MUC5AC production. Chromatin immunoprecipitation analysis demonstrated lack of GR and of p65 (NFκB) binding to composite GRE domains in the MUC5AC promoter following VBP15 exposure of cells, in contrast to Dex. These data demonstrate that VBP15 is a novel anti-mucin agent that mediates the reduction of MUC5AC gene expression differently than the classical glucocorticoid, Dex.
    MeSH term(s) A549 Cells ; Anti-Inflammatory Agents/administration & dosage ; Anti-Inflammatory Agents/pharmacology ; Bronchi/cytology ; Bronchi/drug effects ; Cell Line ; Dexamethasone/administration & dosage ; Dexamethasone/pharmacology ; Dose-Response Relationship, Drug ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; Gene Expression Regulation/drug effects ; Glucocorticoids/administration & dosage ; Glucocorticoids/pharmacology ; Humans ; Inflammation Mediators/metabolism ; Interleukin-1beta/metabolism ; Mucin 5AC/genetics ; Mucins/antagonists & inhibitors ; Mucins/metabolism ; Pregnadienediols/administration & dosage ; Pregnadienediols/pharmacology ; RNA, Messenger/metabolism ; Time Factors
    Chemical Substances Anti-Inflammatory Agents ; Glucocorticoids ; Inflammation Mediators ; Interleukin-1beta ; MUC5AC protein, human ; Mucin 5AC ; Mucins ; Pregnadienediols ; RNA, Messenger ; VBP15 compound ; Dexamethasone (7S5I7G3JQL)
    Language English
    Publishing date 2016-06
    Publishing country England
    Document type Comparative Study ; Journal Article
    ZDB-ID 1399707-5
    ISSN 1522-9629 ; 1094-5539
    ISSN (online) 1522-9629
    ISSN 1094-5539
    DOI 10.1016/j.pupt.2016.04.004
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    Zs.A 2389: Show issues Location:
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  3. Article ; Online: Middle Ear Response of Muc5ac and Muc5b Mucins to Nontypeable Haemophilus influenzae.

    Val, Stéphanie / Kwon, Hyung-Joo / Rose, Mary C / Preciado, Diego

    JAMA otolaryngology-- head & neck surgery

    2015  Volume 141, Issue 11, Page(s) 997–1005

    Abstract: Importance: Chronic otitis media with effusion is characterized by middle ear secretion of mucin glycoproteins, predominantly MUC5B; MUC5AC, the other secretory mucin studied frequently, has also been identified in the middle ear. Emerging evidence ... ...

    Abstract Importance: Chronic otitis media with effusion is characterized by middle ear secretion of mucin glycoproteins, predominantly MUC5B; MUC5AC, the other secretory mucin studied frequently, has also been identified in the middle ear. Emerging evidence suggests a dichotomous role for these mucins in innate immune responses. We hypothesized that MUC5AC is an acute responder and MUC5B is expressed at later time points, reflecting a chronic situation.
    Objective: To determine middle ear regulation of MUC5B and MUC5AC following in vitro bacterial and cytokine exposure.
    Design, setting, and samples: An in vitro cell-based model of mucin gene regulation was conducted in a basic science laboratory at a tertiary pediatric hospital. The study was conducted from July 1, 2014, to June 30, 2015; data analysis was performed in July 2015.
    Interventions: Nontypeable Haemophilus influenzae (NTHi) lysates were generated and used to stimulate mouse middle ear epithelial cells (mMEECs) for 2 hours during 3 weeks.
    Main outcomes and measures: Real-time quantitative polymerase chain reaction, luciferase assays, Western blot assay, and immunofluorescence techniques were performed to determine Muc5ac and Muc5b expression over time, Cxcl2 chemokine response, and nuclear factor-κB activation. Luciferase reporter assays were performed to evaluate specific promoter responses after NTHi exposure.
    Results: Nontypeable H influenzae lysates (200 μg/mL) drove differential mucin gene activation, with Muc5ac being induced up to 2.04 fold at 24 hours and 2.79 fold at 96 hours (P < .05) and Muc5b being induced only at more long-term points: 1.61 fold at 96 hours, 1.41 fold at 1 week, and 1.53 fold at 3 weeks (P < .05). Although NTHi lysates induced robust, early nuclear factor-κB nuclear translocation with nuclear factor-κB-dependent induction of Cxlc2 expression, the lysates had minimal to no effect on Muc5ac and Muc5b promoter activity. However, in contrast to NTHi lysates, CXCL2 induced significant transcription of both Muc5b and Muc5ac as early as 24 hours.
    Conclusions and relevance: Nontypeable H influenzae lysates activate differential mucin gene activation in mMEECs. Although Muc5ac is an early response mucin gene, Muc5b appears to react as a chronic response mucin.
    MeSH term(s) Animals ; Blotting, Western ; Cells, Cultured ; Chemokine CXCL2/biosynthesis ; Ear, Middle/cytology ; Ear, Middle/microbiology ; Epithelial Cells/metabolism ; Epithelial Cells/pathology ; Gene Expression Regulation ; Haemophilus Infections/metabolism ; Haemophilus influenzae/physiology ; Mice ; Mucin 5AC/genetics ; Mucin 5AC/metabolism ; Mucin-5B/genetics ; Mucin-5B/metabolism ; NF-kappa B/metabolism ; Real-Time Polymerase Chain Reaction ; Time Factors ; Transcriptional Activation
    Chemical Substances Chemokine CXCL2 ; Cxcl2 protein, mouse ; Muc5ac protein, mouse ; Muc5b protein, mouse ; Mucin 5AC ; Mucin-5B ; NF-kappa B
    Language English
    Publishing date 2015-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2701825-8
    ISSN 2168-619X ; 2168-6181
    ISSN (online) 2168-619X
    ISSN 2168-6181
    DOI 10.1001/jamaoto.2015.2338
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    Ul II Zs.87: Show issues Location:
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  4. Article ; Online: Proteomic analysis of pediatric sinonasal secretions shows increased MUC5B mucin in CRS.

    Saieg, Amarel / Brown, Kristy J / Pena, Maria T / Rose, Mary C / Preciado, Diego

    Pediatric research

    2015  Volume 77, Issue 2, Page(s) 356–362

    Abstract: Background: Chronic rhinosinusitis (CRS) is characterized by mucous overproduction and submucosal gland hyperplasia. The global protein profile of sinonasal secretions in pediatric CRS has not been studied. We hypothesized that MUC5B, a glandular mucin, ...

    Abstract Background: Chronic rhinosinusitis (CRS) is characterized by mucous overproduction and submucosal gland hyperplasia. The global protein profile of sinonasal secretions in pediatric CRS has not been studied. We hypothesized that MUC5B, a glandular mucin, would be relatively increased in CRS secretions compared to other mucins.
    Methods: Secretions were collected at Children's National Health System (Children's National) from CRS patients undergoing sinus surgery and from control patients without CRS undergoing craniofacial procedures. Proteins were extracted, digested to peptides, and analyzed by mass spectometry. Fold change significance was calculated using the QSpec algorithm. Western blot analysis was performed to validate proteomic findings.
    Results: In total, 294 proteins were identified. Although both MUC5B and MUC5AC were identified in a majority of samples, the relative abundance of MUC5B was found to be significantly higher (P < 0.05). Western blot data validated these findings. Other proteins with the highest significant positive-fold change in CRS samples were BP1 fold-containing family A member 1, chitinase-3-like protein 1, plastin-2, serpin 10, and BP1 fold-containing family B member 1.
    Conclusion: Overall, our data demonstrate an increase of MUC5B abundance in the sinus secretions of pediatric patients with CRS.
    MeSH term(s) Adolescent ; Blotting, Western ; Child ; Child, Preschool ; Electrophoresis, Polyacrylamide Gel ; Gene Ontology ; Humans ; Mucin-5B/secretion ; Mucous Membrane/metabolism ; Paranasal Sinuses/secretion ; Proteomics ; Rhinitis/metabolism ; Sinusitis/metabolism
    Chemical Substances MUC5B protein, human ; Mucin-5B
    Language English
    Publishing date 2015-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 4411-8
    ISSN 1530-0447 ; 0031-3998
    ISSN (online) 1530-0447
    ISSN 0031-3998
    DOI 10.1038/pr.2014.187
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    Zs.A 564: Show issues Location:
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    Zs.MO 373: Show issues

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  5. Article ; Online: Nasopharyngeal microbiome in premature infants and stability during rhinovirus infection.

    Perez, Geovanny F / Pérez-Losada, Marcos / Isaza, Natalia / Rose, Mary C / Colberg-Poley, Anamaris M / Nino, Gustavo

    Journal of investigative medicine : the official publication of the American Federation for Clinical Research

    2017  Volume 65, Issue 6, Page(s) 984–990

    Abstract: Rationale: The nasopharyngeal (NP) microbiota of newborns and infants plays a key role in modulating airway inflammation and respiratory symptoms during viral infections. Premature (PM) birth modifies the early NP environment and is a major risk factor ... ...

    Abstract Rationale: The nasopharyngeal (NP) microbiota of newborns and infants plays a key role in modulating airway inflammation and respiratory symptoms during viral infections. Premature (PM) birth modifies the early NP environment and is a major risk factor for severe viral respiratory infections. However, it is currently unknown if the NP microbiota of PM infants is altered relative to full-term (FT) individuals.
    Objectives: To characterize the NP microbiota differences in preterm and FT infants during rhinovirus (RV) infection.
    Methods: We determined the NP microbiota of infants 6 months to ≤2 years of age born FT (n=6) or severely PM<32 weeks gestation (n=7). We compared microbiota composition in healthy NP samples and performed a longitudinal analysis during naturally occurring RV infections to contrast the microbiota dynamics in PM versus FT infants.
    Results: We observed significant differences in the NP bacterial community of PM versus FT. NP from PM infants had higher within-group dissimilarity (heterogeneity) relative to FT infants. Bacterial composition of NP samples from PM infants showed increased
    Conclusions: PM is associated with NP microbiota changes beyond the neonatal stage. PM infants have an NP microbiota with high heterogeneity relative to FT infants. These prematurity-related microbiota features persisted during RV infection, suggesting that the NP microbiota of PM may play an important role in modulating airway inflammatory and immune responses in this vulnerable group.
    Language English
    Publishing date 2017-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 1217870-6
    ISSN 1708-8267 ; 0009-9279 ; 1081-5589
    ISSN (online) 1708-8267
    ISSN 0009-9279 ; 1081-5589
    DOI 10.1136/jim-2017-000414
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    Zs.A 307: Show issues Location:
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  6. Article ; Online: Human Metapneumovirus Infection is Associated with Severe Respiratory Disease in Preschool Children with History of Prematurity.

    Pancham, Krishna / Sami, Iman / Perez, Geovanny F / Huseni, Shehlanoor / Kurdi, Bassem / Rose, Mary C / Rodriguez-Martinez, Carlos E / Nino, Gustavo

    Pediatrics and neonatology

    2016  Volume 57, Issue 1, Page(s) 27–34

    Abstract: Background: Human metapneumovirus (HMPV) is a recently discovered respiratory pathogen of the family Paramyxoviridae, the same family as that of respiratory syncytial virus (RSV). Premature children are at high risk of severe RSV infections, however, it ...

    Abstract Background: Human metapneumovirus (HMPV) is a recently discovered respiratory pathogen of the family Paramyxoviridae, the same family as that of respiratory syncytial virus (RSV). Premature children are at high risk of severe RSV infections, however, it is unclear whether HMPV infection is more severe in hospitalized children with a history of severe prematurity.
    Methods: We conducted a retrospective analysis of the clinical respiratory presentation of all polymerase chain reaction-confirmed HMPV infections in preschool-age children (≤5 years) with and without history of severe prematurity (<32 weeks gestation). Respiratory distress scores were developed to examine the clinical severity of HMPV infections. Demographic and clinical variables were obtained from reviewing electronic medical records.
    Results: A total of 571 preschool children were identified using polymerase chain reaction-confirmed viral respiratory tract infection during the study period. HMPV was identified as a causative organism in 63 cases (11%). Fifty-eight (n = 58) preschool-age children with HMPV infection were included in this study after excluding those with significant comorbidities. Our data demonstrated that 32.7% of children admitted with HMPV had a history of severe prematurity. Preschool children with a history of prematurity had more severe HMPV disease as illustrated by longer hospitalizations, new or increased need for supplemental O2, and higher severity scores independently of age, ethnicity, and history of asthma.
    Conclusion: Our study suggests that HMPV infection causes significant disease burden among preschool children with a history of prematurity leading to severe respiratory infections and increasing health care resource utilization due to prolonged hospitalizations.
    MeSH term(s) Child, Hospitalized ; Child, Preschool ; Cross-Sectional Studies ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Metapneumovirus ; Paramyxoviridae Infections/complications ; Premature Birth/epidemiology ; Respiratory Syncytial Virus Infections/complications ; Respiratory Tract Infections/etiology ; Retrospective Studies
    Language English
    Publishing date 2016-02
    Publishing country Singapore
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2441816-X
    ISSN 2212-1692 ; 1875-9572
    ISSN (online) 2212-1692
    ISSN 1875-9572
    DOI 10.1016/j.pedneo.2015.03.008
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    Zs.B 1083: Show issues Location:
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  7. Article ; Online: Different next generation sequencing platforms produce different microbial profiles and diversity in cystic fibrosis sputum.

    Hahn, Andrea / Sanyal, Amit / Perez, Geovanny F / Colberg-Poley, Anamaris M / Campos, Joseph / Rose, Mary C / Pérez-Losada, Marcos

    Journal of microbiological methods

    2016  Volume 130, Page(s) 95–99

    Abstract: Background: Cystic fibrosis (CF) is an autosomal recessive disease characterized by recurrent lung infections. Studies of the lung microbiome have shown an association between decreasing diversity and progressive disease. 454 pyrosequencing has ... ...

    Abstract Background: Cystic fibrosis (CF) is an autosomal recessive disease characterized by recurrent lung infections. Studies of the lung microbiome have shown an association between decreasing diversity and progressive disease. 454 pyrosequencing has frequently been used to study the lung microbiome in CF, but will no longer be supported. We sought to identify the benefits and drawbacks of using two state-of-the-art next generation sequencing (NGS) platforms, MiSeq and PacBio RSII, to characterize the CF lung microbiome. Each has its advantages and limitations.
    Methods: Twelve samples of extracted bacterial DNA were sequenced on both MiSeq and PacBio NGS platforms. DNA was amplified for the V4 region of the 16S rRNA gene and libraries were sequenced on the MiSeq sequencing platform, while the full 16S rRNA gene was sequenced on the PacBio RSII sequencing platform. Raw FASTQ files generated by the MiSeq and PacBio platforms were processed in mothur v1.35.1.
    Results: There was extreme discordance in alpha-diversity of the CF lung microbiome when using the two platforms. Because of its depth of coverage, sequencing of the 16S rRNA V4 gene region using MiSeq allowed for the observation of many more operational taxonomic units (OTUs) and higher Chao1 and Shannon indices than the PacBio RSII. Interestingly, several patients in our cohort had Escherichia, an unusual pathogen in CF. Also, likely because of its coverage of the complete 16S rRNA gene, only PacBio RSII was able to identify Burkholderia, an important CF pathogen.
    Conclusion: When comparing microbiome diversity in clinical samples from CF patients using 16S sequences, MiSeq and PacBio NGS platforms may generate different results in microbial community composition and structure. It may be necessary to use different platforms when trying to correctly identify dominant pathogens versus measuring alpha-diversity estimates, and it would be important to use the same platform for comparisons to minimize errors in interpretation.
    MeSH term(s) Bacteria/classification ; Bacteria/genetics ; Bacteria/pathogenicity ; Base Sequence ; Biodiversity ; Classification ; Computational Biology/methods ; Cystic Fibrosis/microbiology ; DNA, Bacterial/genetics ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Lung/microbiology ; Metagenome ; Microbiota/genetics ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Sputum/microbiology
    Chemical Substances DNA, Bacterial ; RNA, Ribosomal, 16S
    Language English
    Publishing date 2016-11
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2016.09.002
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    Zs.A 1849: Show issues Location:
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  8. Article ; Online: Cigarette smoke alters tissue inhibitor of metalloproteinase 1 and matrix metalloproteinase 9 levels in the basolateral secretions of human asthmatic bronchial epithelium in vitro.

    Watson, Alan M / Benton, Angela S / Rose, Mary C / Freishtat, Robert J

    Journal of investigative medicine : the official publication of the American Federation for Clinical Research

    2010  Volume 58, Issue 5, Page(s) 725–729

    Abstract: Background: Asthma, a major cause of chronic lung disease worldwide, has increased in prevalence in all age and ethnic groups, particularly in urban areas where cigarette smoking is common. Cigarette smoke (CS) significantly impacts the development of ... ...

    Abstract Background: Asthma, a major cause of chronic lung disease worldwide, has increased in prevalence in all age and ethnic groups, particularly in urban areas where cigarette smoking is common. Cigarette smoke (CS) significantly impacts the development of asthma and is strongly associated with increased asthma-related morbidity.
    Purpose: To evaluate bioinformatic analyses predicting that CS would alter expression of tissue inhibitor of metalloproteinase (TIMP) 1 and matrix metalloproteinase (MMP) 9 in asthmatic epithelium.
    Methods: Primary differentiated normal (n = 4) and asthmatic (n = 4) human respiratory epithelia on collagen-coated Transwells at air-liquid interface were exposed for 1 hour to CS condensate (CSC) or hydrogen peroxide (H2O2). Tissue inhibitor of metalloproteinase 1 and MMP-9 protein levels were measured at 24 hours by enzyme-linked immunosorbent assay in cell lysates and in apical and basolateral secretions.
    Results: Tissue inhibitor of metalloproteinase 1 and MMP-9 levels in the apical secretions of normal and asthmatic epithelia were unchanged after exposure to CSC and H2O2. However, CSC increased TIMP-1 levels in the basolateral secretions of both normal and asthmatic epithelia, but decreased MMP-9 levels only in asthmatic basolateral secretions, resulting in a 2.5-fold lower MMP-9/TIMP-1 ratio that corresponded to decreased MMP-9 activity in CS-exposed asthmatic basolateral secretions.
    Conclusions: These data validate our prior bioinformatic analyses predicting that TIMP-1 plays a role in the stress response to CS and indicate that asthmatics exposed to CS may be more susceptible to MMP-9-mediated airway remodeling. This is in agreement with the current paradigm that a reduction in the MMP-9/TIMP-1 ratio is a milieu that favors subepithelial airway remodeling in chronic asthma.
    MeSH term(s) Adult ; Asthma/enzymology ; Asthma/pathology ; Bronchi ; Cells, Cultured ; Child ; Child, Preschool ; Computational Biology ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Male ; Matrix Metalloproteinase 9/metabolism ; Middle Aged ; Physiology ; Respiratory Mucosa/drug effects ; Respiratory Mucosa/enzymology ; Respiratory Mucosa/pathology ; Smoking/adverse effects ; Tissue Inhibitor of Metalloproteinase-1/metabolism ; Young Adult
    Chemical Substances Tissue Inhibitor of Metalloproteinase-1 ; Matrix Metalloproteinase 9 (EC 3.4.24.35)
    Language English
    Publishing date 2010-07-01
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1217870-6
    ISSN 1708-8267 ; 0009-9279 ; 1081-5589
    ISSN (online) 1708-8267
    ISSN 0009-9279 ; 1081-5589
    DOI 10.231/JIM.0b013e3181db874e
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  9. Article ; Online: Rhinovirus-induced airway cytokines and respiratory morbidity in severely premature children.

    Perez, Geovanny F / Pancham, Krishna / Huseni, Shehlanoor / Jain, Amisha / Rodriguez-Martinez, Carlos E / Preciado, Diego / Rose, Mary C / Nino, Gustavo

    Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

    2015  Volume 26, Issue 2, Page(s) 145–152

    Abstract: Background: Rhinovirus (RV) has been linked to the pathogenesis of asthma. Prematurity is a risk factor for severe RV infection in early life, but is unknown if RV elicits enhanced pro-asthmatic airway cytokine responses in premature infants. This study ...

    Abstract Background: Rhinovirus (RV) has been linked to the pathogenesis of asthma. Prematurity is a risk factor for severe RV infection in early life, but is unknown if RV elicits enhanced pro-asthmatic airway cytokine responses in premature infants. This study investigated whether young children born severely premature (<32 wks gestation) exhibit airway secretion of Th2 and Th17 cytokines during natural RV infections and whether RV-induced Th2-Th17 responses are linked to more respiratory morbidity in premature children during the first 2 yrs of life.
    Methods: We measured Th2 and Th17 nasal airway cytokines in a retrospective cohort of young children aged 0-2 yrs with PCR-confirmed RV infection or non-detectable virus. Protein levels of IL-4, IL-13, TSLP, and IL-17 were determined with multiplex immunoassays. Demographic and clinical variables were obtained by electronic medical record (EMR) review.
    Results: The study comprised 214 children born full term (n = 108), preterm (n = 44) or severely premature (n = 62). Natural RV infection in severely premature children was associated with elevated airway secretion of Th2 (IL-4 and IL-13) and Th17 (IL-17) cytokines, particularly in subjects with history of bronchopulmonary dysplasia. Severely premature children with high RV-induced airway IL-4 had recurrent respiratory hospitalizations (median 3.65 hosp/yr; IQR 2.8-4.8) and were more likely to have at least one pediatric intensive care unit admission during the first 2 yrs of life (OR 8.72; 95% CI 1.3-58.7; p = 0.02).
    Conclusions: Severely premature children have increased airway secretion of Th2 and Th17 cytokines during RV infections, which is associated with more respiratory morbidity in the first 2 yrs of life.
    MeSH term(s) Asthma/immunology ; Asthma/virology ; Bronchopulmonary Dysplasia/immunology ; Bronchopulmonary Dysplasia/virology ; Cohort Studies ; Common Cold/complications ; Common Cold/immunology ; Cytokines/biosynthesis ; Cytokines/immunology ; Female ; Humans ; Infant, Extremely Premature/immunology ; Infant, Newborn ; Infant, Premature ; Male ; Multiplex Polymerase Chain Reaction ; Respiratory System/immunology ; Respiratory System/virology ; Retrospective Studies ; Rhinovirus
    Chemical Substances Cytokines
    Language English
    Publishing date 2015-03
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1057059-7
    ISSN 1399-3038 ; 0905-6157 ; 0906-5784
    ISSN (online) 1399-3038
    ISSN 0905-6157 ; 0906-5784
    DOI 10.1111/pai.12346
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  10. Article ; Online: Quantitative proteomics reveals an altered cystic fibrosis in vitro bronchial epithelial secretome.

    Peters-Hall, Jennifer R / Brown, Kristy J / Pillai, Dinesh K / Tomney, Amarel / Garvin, Lindsay M / Wu, Xiaofang / Rose, Mary C

    American journal of respiratory cell and molecular biology

    2015  Volume 53, Issue 1, Page(s) 22–32

    Abstract: Alterations in epithelial secretions and mucociliary clearance contribute to chronic bacterial infection in cystic fibrosis (CF) lung disease, but whether CF lungs are unchanged in the absence of infection remains controversial. A proteomic comparison of ...

    Abstract Alterations in epithelial secretions and mucociliary clearance contribute to chronic bacterial infection in cystic fibrosis (CF) lung disease, but whether CF lungs are unchanged in the absence of infection remains controversial. A proteomic comparison of airway secretions from subjects with CF and control subjects shows alterations in key biological processes, including immune response and proteolytic activity, but it is unclear if these are due to mutant CF transmembrane conductance regulator (CFTR) and/or chronic infection. We hypothesized that the CF lung apical secretome is altered under constitutive conditions in the absence of inflammatory cells and pathogens. To test this, we performed quantitative proteomics of in vitro apical secretions from air-liquid interface cultures of three life-extended CF (ΔF508/ΔF508) and three non-CF human bronchial epithelial cells after labeling of CF cells by stable isotope labeling with amino acids in cell culture. Mass spectrometry analysis identified and quantitated 666 proteins across samples, of which 70 exhibited differential enrichment or depletion in CF secretions (±1.5-fold change; P < 0.05). The key molecular functions were innate immunity (24%), cytoskeleton/extracellular matrix organization (24%), and protease/antiprotease activity (17%). Oxidative proteins and classical complement pathway proteins that are altered in CF secretions in vivo were not altered in vitro. Specific differentially increased proteins-MUC5AC and MUC5B mucins, fibronectin, and matrix metalloproteinase-9-were validated by antibody-based assays. Overall, the in vitro CF secretome data are indicative of a constitutive airway epithelium with altered innate immunity, suggesting that downstream consequences of mutant CFTR set the stage for chronic inflammation and infection in CF airways.
    MeSH term(s) Bronchi/metabolism ; Bronchi/pathology ; Cell Line ; Chronic Disease ; Cystic Fibrosis/genetics ; Cystic Fibrosis/metabolism ; Cystic Fibrosis/pathology ; Humans ; Inflammation/genetics ; Inflammation/metabolism ; Inflammation/pathology ; Proteome/genetics ; Proteome/metabolism ; Proteomics ; Respiratory Mucosa/metabolism ; Respiratory Mucosa/pathology
    Chemical Substances Proteome
    Language English
    Publishing date 2015-02-18
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1025960-0
    ISSN 1535-4989 ; 1044-1549
    ISSN (online) 1535-4989
    ISSN 1044-1549
    DOI 10.1165/rcmb.2014-0256RC
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