Article ; Online: Azurin-poly(N-isopropylacrylamide) conjugates by site-directed mutagenesis and their thermosensitive behavior in electron-transfer processes.
Angewandte Chemie (International ed. in English)
2009 Volume 48, Issue 11, Page(s) 1946–1949
Abstract: Searching for "intelligence": Azurin-PNIPAM conjugates were prepared by site-directed mutagenesis followed by protein reconstitution by using imidazole-conjugated poly(N-isopropylacrylamides). The polymer-bound imidazole acts as a ligand in the active ... ...
| Abstract | Searching for "intelligence": Azurin-PNIPAM conjugates were prepared by site-directed mutagenesis followed by protein reconstitution by using imidazole-conjugated poly(N-isopropylacrylamides). The polymer-bound imidazole acts as a ligand in the active site of the blue copper protein azurin. The bioconjugates showed thermosensitive behavior in electron-transfer processes with reduced cytochrome c. |
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| MeSH term(s) | Acrylamides/chemical synthesis ; Acrylamides/chemistry ; Acrylic Resins ; Azurin/chemical synthesis ; Azurin/chemistry ; Azurin/genetics ; Circular Dichroism ; Copper/chemistry ; Electron Spin Resonance Spectroscopy ; Electron Transport ; Mutagenesis, Site-Directed ; Polymers/chemical synthesis ; Polymers/chemistry ; Protein Engineering ; Spectrophotometry, Ultraviolet |
| Chemical Substances | Acrylamides ; Acrylic Resins ; Polymers ; Azurin (12284-43-4) ; poly-N-isopropylacrylamide (25189-55-3) ; Copper (789U1901C5) |
| Language | English |
| Publishing date | 2009 |
| Publishing country | Germany |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2011836-3 |
| ISSN | 1521-3773 ; 1433-7851 |
| ISSN (online) | 1521-3773 |
| ISSN | 1433-7851 |
| DOI | 10.1002/anie.200804440 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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