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  1. Article: Evolving a New Efficient Mode of Fructose Utilization for Improved Bioproduction in

    Krahn, Irene / Bonder, Daniel / Torregrosa-Barragán, Lucía / Stoppel, Dominik / Krause, Jens P / Rosenfeldt, Natalie / Meiswinkel, Tobias M / Seibold, Gerd M / Wendisch, Volker F / Lindner, Steffen N

    Frontiers in bioengineering and biotechnology

    2021  Volume 9, Page(s) 669093

    Abstract: Fructose utilization ... ...

    Abstract Fructose utilization in
    Language English
    Publishing date 2021-05-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2021.669093
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Production of the compatible solute α-D-glucosylglycerol by metabolically engineered Corynebacterium glutamicum.

    Roenneke, Benjamin / Rosenfeldt, Natalie / Derya, Sami M / Novak, Jens F / Marin, Kay / Krämer, Reinhard / Seibold, Gerd M

    Microbial cell factories

    2018  Volume 17, Issue 1, Page(s) 94

    Abstract: Background: α-D-Glucosylglycerol (αGG) has beneficial functions as a moisturizing agent in cosmetics and potential as a health food material, and therapeutic agent. αGG serves as compatible solute in various halotolerant cyanobacteria such as ... ...

    Abstract Background: α-D-Glucosylglycerol (αGG) has beneficial functions as a moisturizing agent in cosmetics and potential as a health food material, and therapeutic agent. αGG serves as compatible solute in various halotolerant cyanobacteria such as Synechocystis sp. PCC 6803, which synthesizes αGG in a two-step reaction: The enzymatic condensation of ADP-glucose and glycerol 3-phosphate by GG-phosphate synthase (GGPS) is followed by the dephosphorylation of the intermediate by the GG-phosphate phosphatase (GGPP). The Gram-positive Corynebacterium glutamicum, an industrial workhorse for amino acid production, does not utilize αGG as a substrate and was therefore chosen for the development of a heterologous microbial production platform for αGG.
    Results: Plasmid-bound expression of ggpS and ggpP from Synechocystis sp. PCC 6803 enabled αGG synthesis exclusively in osmotically stressed cells of C. glutamicum (pEKEx2-ggpSP), which is probably due to the unique intrinsic control mechanism of GGPS activity in response to intracellular ion concentrations. C. glutamicum was then engineered to optimize precursor supply for αGG production: The precursor for αGG synthesis ADP-glucose gets metabolized by both the glgA encoded glycogen synthase and the otsA encoded trehalose-6-phosphate synthase. Upon deletion of both genes the αGG concentration in culture supernatants was increased from 0.5 mM in C. glutamicum (pEKEx3-ggpSP) to 2.9 mM in C. glutamicum ΔotsA IMglgA (pEKEx3-ggpSP). Upon nitrogen limitation, which inhibits synthesis of amino acids as compatible solutes, C. glutamicum ΔotsA IMglgA (pEKEx3-ggpSP) produced more than 10 mM αGG (about 2 g L
    Conclusions: Corynebacterium glutamicum can be engineered as efficient platform for the production of the compatible solute αGG. Redirection of carbon flux towards αGG synthesis by elimination of the competing pathways for glycogen and trehalose synthesis as well as optimization of nitrogen supply is an efficient strategy to further optimize production of αGG.
    MeSH term(s) Corynebacterium glutamicum/metabolism ; Glucosides/metabolism ; Glycogen/metabolism ; Metabolic Engineering/methods ; Trehalose/metabolism
    Chemical Substances Glucosides ; glucosylglycerol ; Glycogen (9005-79-2) ; Trehalose (B8WCK70T7I)
    Language English
    Publishing date 2018-06-16
    Publishing country England
    Document type Journal Article
    ISSN 1475-2859
    ISSN (online) 1475-2859
    DOI 10.1186/s12934-018-0939-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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