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  1. Book ; Thesis: Neue Cumarin-Substrate für Cytochrom P450-abhängige Monooxygenasen

    Roser, René

    Entwicklung von direkt-fluorometrischen Tests zur Bestimmung der Enzymaktivität

    1997  

    Author's details René Roser
    Language German
    Size 81 S. : graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Ulm, Univ., Diss., 1998
    HBZ-ID HT008501670
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    98 E 984 order for loan Magazin

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  2. Book ; Thesis: Computertomographische und klinische Untersuchung nach lumbalen Bandscheibenoperationen

    Roser, Ralf

    1986  

    Size 68 S. : Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Giessen, Univ., Diss., 1988
    HBZ-ID HT003176720
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

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  3. Article: A Direct, Highly Sensitive Fluorometric Assay for a Microsomal Cytochrome P450-Mediated O-Demethylation Using a Novel Coumarin Analog as Substrate

    Roser, René / Helmut Thomas

    Zeitschrift für Naturforschung C. 2014 June 2, v. 55, no. 11-12

    2014  

    Abstract: A highly sensitive fluorometric assay for the determination of monooxygenase activity in liver microsomes is described. The assay is based on the use of 3-chloro-7-methoxy-4-methylcoumarin which is demethylated to 3-chloro-7-hydroxy-4-methylcoumarin. The ...

    Abstract A highly sensitive fluorometric assay for the determination of monooxygenase activity in liver microsomes is described. The assay is based on the use of 3-chloro-7-methoxy-4-methylcoumarin which is demethylated to 3-chloro-7-hydroxy-4-methylcoumarin. The rate of formation of 3-chloro-7-hydroxy-4-methylcoumarin was recorded as an increase of fluorescence (λA = 380 nm, λF = 480 nm) with time. When 3-chloro-7-methoxy-4-methylcoumarin was incubated in the presence of MgCl₂and NADPH with rat liver microsomes, a continuous increase of the fluorescence could be measured. The reaction proceeded linearly for about 10 min and at least up to a concentration of 0.1 mg/ml of microsomal protein. Besides 3-chloro-7-hydroxy-4-methylcoumarin a hydroxylated derivative of the substrate was formed as a second metabolite during the incubation. Using an excitation wavelength of 480 nm and a fluorescence/em ission w avelength of 480 nm, the fluorescence of this substance (λA = 338 nm, λF = 422 nm) amounted only to about 1% of the fluorescence of the main product. The use of 3-chloro-7-methoxy-4-methylcoumarin as substrate enables the fluorometric determination of the O-dealkylation activity of a cytochrom e P450-dependent monooxygenase system in rat liver which is inducible by phenobarbital but not by 3-methylcholanthrene.
    Keywords coumarin ; fluorescence ; fluorometry ; liver ; liver microsomes ; metabolites ; NADP (coenzyme) ; phenobarbital ; rats ; wavelengths
    Language English
    Dates of publication 2014-0602
    Size p. 915-922.
    Publishing place Verlag der Zeitschrift für Naturforschung
    Document type Article
    ZDB-ID 124636-7
    ISSN 1865-7125 ; 0341-0382 ; 0341-0471 ; 0939-5075
    ISSN (online) 1865-7125
    ISSN 0341-0382 ; 0341-0471 ; 0939-5075
    DOI 10.1515/znc-2000-11-1212
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4' 47/11 c: Show issues
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  4. Article: A direct, highly sensitive fluorometric assay for a microsomal cytochrome P450-mediated O-demethylation using a novel coumarin analog as substrate.

    Roser, R / Thomas, H

    Zeitschrift fur Naturforschung. C, Journal of biosciences

    2000  Volume 55, Issue 11-12, Page(s) 915–922

    Abstract: A highly sensitive fluorometric assay for the determination of monooxygenase activity in liver microsomes is described. The assay is based on the use of 3-chloro-7-methoxy-4-methylcoumarin which is demethylated to 3-chloro-7-hydroxy-4-methylcoumarin. The ...

    Abstract A highly sensitive fluorometric assay for the determination of monooxygenase activity in liver microsomes is described. The assay is based on the use of 3-chloro-7-methoxy-4-methylcoumarin which is demethylated to 3-chloro-7-hydroxy-4-methylcoumarin. The rate of formation of 3-chloro-7-hydroxy-4-methylcoumarin was recorded as an increase of fluorescence (lambdaA = 380 nm, lambdaF = 480 nm) with time. When 3-chloro-7-methoxy-4-methylcoumarin was incubated in the presence of MgCl2 and NADPH with rat liver microsomes, a continuous increase of the fluorescence could be measured. The reaction proceeded linearly for about 10 min and at least up to a concentration of 0.1 mg/ml of microsomal protein. Besides 3-chloro-7-hydroxy-4-methylcoumarin a hydroxylated derivative of the substrate was formed as a second metabolite during the incubation. Using an excitation wavelength of 380 nm and a fluorescence/emission wavelength of 480 nm, the fluorescence of this substance (lambdaA = 338 nm, lambdaF = 422 nm) amounted only to about 1% of the fluorescence of the main product. The use of 3-chloro-7-methoxy-4-methylcoumarin as substrate enables the fluorometric determination of the O-dealkylation activity of a cytochrome P450-dependent monooxygenase system in rat liver which is inducible by phenobarbital but not by 3-methylcholanthrene.
    MeSH term(s) Animals ; Cytochrome P-450 Enzyme System/analysis ; Cytochrome P-450 Enzyme System/metabolism ; Enzyme Inhibitors/pharmacology ; Hydroxylation ; Kinetics ; Male ; Methylcholanthrene/pharmacology ; Microsomes, Liver/drug effects ; Microsomes, Liver/enzymology ; Oxidoreductases, O-Demethylating/analysis ; Oxidoreductases, O-Demethylating/metabolism ; Phenobarbital/pharmacology ; Rats ; Rats, Sprague-Dawley ; Sensitivity and Specificity ; Spectrometry, Fluorescence/methods
    Chemical Substances Enzyme Inhibitors ; Methylcholanthrene (56-49-5) ; Cytochrome P-450 Enzyme System (9035-51-2) ; Oxidoreductases, O-Demethylating (EC 1.-) ; Phenobarbital (YQE403BP4D)
    Language English
    Publishing date 2000-11
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 124636-7
    ISSN 1865-7125 ; 0939-5075 ; 0341-0382 ; 0341-0471
    ISSN (online) 1865-7125
    ISSN 0939-5075 ; 0341-0382 ; 0341-0471
    DOI 10.1515/znc-2000-11-1212
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 962: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  5. Article: Zur Problematik des idiopathischen Chylothorax*

    Roser, R. / Große-Thie, W. / Stallkamp, B.

    DMW - Deutsche Medizinische Wochenschrift

    2008  Volume 113, Issue 49, Page(s) 1916–1919

    Abstract: Bei einem 57jährigen Patienten fand sich als Ursache rechtsseitiger atemabhängiger Thoraxschmerzen ein Chylothorax. Eine dreiwöchige konservative Behandlung, bei der insgesamt 25 Liter Chylus entleert wurden, führte nicht zur Besserung. Nach Thorakotomie ...

    Abstract Bei einem 57jährigen Patienten fand sich als Ursache rechtsseitiger atemabhängiger Thoraxschmerzen ein Chylothorax. Eine dreiwöchige konservative Behandlung, bei der insgesamt 25 Liter Chylus entleert wurden, führte nicht zur Besserung. Nach Thorakotomie mit Ligatur und Resektion des Ductus thoracicus kam es zu sofortigem Sistieren des Lymphflusses. Weder eine umfangreiche präoperative Diagnostik noch die histologische Untersuchung des Lymphganges konnten die Krankheitsurache klären.
    Language German
    Publishing date 2008-03-25
    Publishing place Stuttgart ; New York
    Document type Article
    ZDB-ID 200446-x
    ISSN 1439-4413 ; 0012-0472
    ISSN (online) 1439-4413
    ISSN 0012-0472
    DOI 10.1055/s-2008-1067912
    Database Thieme publisher's database

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    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.60: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Ua VI Zs.60/X: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  6. Book ; Thesis: Neue Cumarin-Substrate für Cytochrom P450-abhängige Monooxygenasen

    Roser, René

    Entwicklung von direkt-fluorometrischen Tests zur Bestimmung der Enzymaktivität

    1997  

    Author's details René Roser
    Language German
    Size 81 S, graph. Darst, 30 cm
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., Diss.--Ulm, 1998
    Database Former special subject collection: coastal and deep sea fishing

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  7. Article: A Direct, Highly Sensitive Fluorometric Assay for a Microsomal Cytochrome P 450-Mediated Demethylation Using a Novel Coumarin Analog as Substrate

    Roser, R. / Thomas, H.

    Zeitschrift für Naturforschung : Section C

    2000  Volume 55, Issue 11/12, Page(s) 915

    Language German
    Document type Article
    ZDB-ID 124636-7
    ISSN 0341-0382 ; 0341-0471
    Database Current Contents Medicine

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 962: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  8. Book: Eins von hundert Jahren

    Hermes, Michael / Röser, Hubert / Röser, Richard

    das Jubiläum von DJK Altendorf 09

    2010  

    Institution DJK Altendorf 09
    Author's details [Text: Michael Hermes; Hubert Röser; Richard Röser]
    Keywords DJK Altendorf 09 ; Essen ; Geschichte 2009
    Size 73 Seiten: zahlreiche Illustrationen
    Publisher Verl. für Regionalgeschichte; Gütersloh
    Document type Book
    HBZ-ID HT016367047
    ISBN 978-3-89534-749-8 ; 3-89534-749-3
    Database Central Library of Sport Science of the German Sport University Cologne

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  9. Book ; Thesis: Computertomographische und klinische Untersuchung nach lumbalen Bandscheibenoperationen

    Roser, Ralf

    1986  

    Author's details Ralf Roser
    Language Undetermined
    Size 68 S, Ill., graph. Darst, 21 cm
    Document type Book ; Thesis
    Thesis / German Habilitation thesis @Giessen, Univ., Diss. : 1988
    Database Former special subject collection: coastal and deep sea fishing

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    Inter-library loan at ZB MED

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  10. Article: A study of reference parameters in the CFY (remote Sprague-Dawley) rat.

    Demestre, I / Sagarra, R / Palacios, G / Roser, R

    Methods and findings in experimental and clinical pharmacology

    1989  Volume 11, Issue 2, Page(s) 101–109

    Abstract: The reference parameters for 10- and 19-week old CFY (remote Sprague-Dawley) rats fed powdered feed have been studied: growth curves, food consumption, ophthalmoscopy, results of urinalyses and hematological and biochemical assays, and absolute and ... ...

    Abstract The reference parameters for 10- and 19-week old CFY (remote Sprague-Dawley) rats fed powdered feed have been studied: growth curves, food consumption, ophthalmoscopy, results of urinalyses and hematological and biochemical assays, and absolute and relative organ weights. Detailed information is given of instrumentation and methods employed, of the animals, strain and environment, and of blood-sampling technique. In general, the results obtained were similar to those previously reported in literature.
    MeSH term(s) Animals ; Behavior, Animal ; Body Weight/drug effects ; Eating/drug effects ; Eye/drug effects ; Female ; Globulins/metabolism ; Leukocyte Count ; Male ; Organ Size/drug effects ; Rats ; Rats, Inbred Strains ; Reference Standards ; Urine/analysis
    Chemical Substances Globulins
    Language English
    Publishing date 1989-02
    Publishing country Spain
    Document type Journal Article
    ZDB-ID 446847-8
    ISSN 2013-0155 ; 0379-0355
    ISSN (online) 2013-0155
    ISSN 0379-0355
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1525: Show issues Location:
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