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  1. Article: Metagenomic Predictions: A Review 10 years on.

    Ross, Elizabeth M / Hayes, Ben J

    Frontiers in genetics

    2022  Volume 13, Page(s) 865765

    Abstract: Metagenomic predictions use variation in the metagenome (microbiome profile) to predict the unknown phenotype of the associated host. Metagenomic predictions were first developed 10 years ago, where they were used to predict which cattle would produce ... ...

    Abstract Metagenomic predictions use variation in the metagenome (microbiome profile) to predict the unknown phenotype of the associated host. Metagenomic predictions were first developed 10 years ago, where they were used to predict which cattle would produce high or low levels of enteric methane. Since then, the approach has been applied to several traits and species including residual feed intake in cattle, and carcass traits, body mass index and disease state in pigs. Additionally, the method has been extended to include predictions based on other multi-dimensional data such as the metabolome, as well to combine genomic and metagenomic information. While there is still substantial optimisation required, the use of metagenomic predictions is expanding as DNA sequencing costs continue to fall and shows great promise particularly for traits heavily influenced by the microbiome such as feed efficiency and methane emissions.
    Language English
    Publishing date 2022-07-20
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2022.865765
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A novel remote assessment pathway to streamline the management of two-week-wait suspected head and neck cancer referrals: a prospective analysis of 660 patients.

    Metcalfe, Christopher / Oh, Soo / Glazzard, Nina / Ross, Elizabeth / George, Ajith

    The Journal of laryngology and otology

    2024  , Page(s) 1–5

    Abstract: Objective: This study analyses outcomes for 660 patients managed via a novel telescopic pathway for suspected head and neck cancer referrals.: Method: Data were collected prospectively between January 2021 and December 2022, capturing all two-week- ... ...

    Abstract Objective: This study analyses outcomes for 660 patients managed via a novel telescopic pathway for suspected head and neck cancer referrals.
    Method: Data were collected prospectively between January 2021 and December 2022, capturing all two-week-wait referrals triaged as low risk and managed via a nurse-led clinic for nasendoscopic examination and consultant-led remote assessment.
    Results: In total, 660 patients were included. There were six head and neck cancers diagnosed, giving a conversion rate of 0.9 per cent. Mean (standard deviation) time to informing the patient whether they did or did not have cancer (28-day faster diagnosis standard) was 28.6 days (20.2), with no significant difference observed in patients imaged prior to review (
    Conclusion: Low-risk head and neck cancer referrals can be safely managed in a nurse-led clinic for recorded examination with asynchronous consultant-led management. Further work is required to ensure adherence to the new faster diagnosis standard.
    Language English
    Publishing date 2024-01-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 218299-3
    ISSN 1748-5460 ; 0022-2151
    ISSN (online) 1748-5460
    ISSN 0022-2151
    DOI 10.1017/S002221512400015X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Dilutional Effect of Ethylenediaminetetraacetic Acid on Packed Cell Volume in Healthy Dogs.

    Ross, Elizabeth H / Dickinson, Amy

    Journal of the American Animal Hospital Association

    2021  Volume 57, Issue 5, Page(s) 199–204

    Abstract: Packed cell volume (PCV) is commonly used to assess and monitor red blood cell count in animals, but the results can be altered if inappropriate ratios of anticoagulant/blood are used. The purpose of this study was to determine the effect of ideally ... ...

    Abstract Packed cell volume (PCV) is commonly used to assess and monitor red blood cell count in animals, but the results can be altered if inappropriate ratios of anticoagulant/blood are used. The purpose of this study was to determine the effect of ideally filled, overfilled, and underfilled K3 ethylenediaminetetraacetic acid (EDTA) tubes with various volumes of healthy dog blood on centrifuged PCV. Six milliliters of blood was obtained from 94 blood donors each. Initial distribution was injected into two nonheparinized microhematocrit tubes. The remainder was instilled into 1.3 mL K3 EDTA spray-dried tubes as 1.5 mL, 1.3 mL, 0.75 mL, 0.5 mL, and 0.25 mL aliquots. Normality was determined using the D'agostino-Pearson method and by visual examination of histograms. Data were analyzed using a repeated-measures analysis of variance with post hoc testing using Tukey's test. There is a statistically significant decrease in the PCV between all groups with progressive underfilling of tubes (P < .0001). The closest difference is between 1.5 and 1.3 mL (P = .0138). Our study suggested that underfilling K3 EDTA tubes significantly and negatively influences the PCV in healthy dogs. Using underfilled K3 EDTA tubes result in a lower PCV compared with directly filled microhematocrit tubes without anticoagulant.
    MeSH term(s) Animals ; Anticoagulants/pharmacology ; Cell Size ; Dog Diseases ; Dogs ; Edetic Acid ; Hematocrit/veterinary
    Chemical Substances Anticoagulants ; Edetic Acid (9G34HU7RV0)
    Language English
    Publishing date 2021-08-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 410383-x
    ISSN 1547-3317 ; 1062-8266 ; 0587-2871
    ISSN (online) 1547-3317
    ISSN 1062-8266 ; 0587-2871
    DOI 10.5326/JAAHA-MS-7060
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Elastic Chain Premaxillary Retraction Appliance Does Not Increase Inter-Canthal Dimension in Patients with Bilateral Cleft Lip and Palate.

    Burashed, Hamad / Resnick, Cory M / Ross, Elizabeth E / Mulliken, John B / Padwa, Bonnie L

    The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association

    2024  , Page(s) 10556656241241200

    Abstract: Objective: To determine if the elastic chain premaxillary retraction (ECPR) appliance increases inter-medial and inter-lateral canthal dimension in patients with bilateral complete cleft lip and palate (BCLP).: Design: Retrospective cohort study.: ... ...

    Abstract Objective: To determine if the elastic chain premaxillary retraction (ECPR) appliance increases inter-medial and inter-lateral canthal dimension in patients with bilateral complete cleft lip and palate (BCLP).
    Design: Retrospective cohort study.
    Setting: Specialized tertiary care facility.
    Patients, participants: 126 patients with BCLP; 75 had ECPR, 51 had no pre-surgical manipulation.
    Interventions: Three-dimensional facial photographs were obtained prior to insertion of appliance (T0), post-appliance therapy prior to appliance removal/labial repair (T1), and several months after labial repair (T2) for a longitudinal ECPR group, and were obtained after age 4 years (T3) for a non-longitudinal ECPR group and for the non-ECPR group.
    Main outcome measures: Inter-medial and inter-lateral canthal dimension (en-en, ex-ex) was determined for all groups/time-points. Measurements were compared between groups and to norms.
    Results: The mean en-en and ex-ex was 32.6 ± 3.2 mm and 84.4 ± 6.3 mm for the ECPR group and 33.5 ± 3.1 mm and 86.7 ± 7.2 mm for the non-ECPR group at T3. Inter-medial and inter-lateral canthal dimensions were significantly greater than normal (
    Conclusions: Inter-medial and inter-lateral canthal dimension increased after ECPR but returned to baseline growth trajectory. These dimensions were above normal at all time-points. There was no difference between those that did and did not have dentofacial orthopedic manipulation.
    Language English
    Publishing date 2024-03-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1069409-2
    ISSN 1545-1569 ; 0009-8701 ; 1055-6656
    ISSN (online) 1545-1569
    ISSN 0009-8701 ; 1055-6656
    DOI 10.1177/10556656241241200
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Non-pharmacologic therapies in treatment of childhood-onset systemic lupus erythematosus: A systematic review.

    Ross, Elizabeth / Abulaban, Khalid / Kessler, Elizabeth / Cunningham, Natoshia

    Lupus

    2022  Volume 31, Issue 7, Page(s) 864–879

    Abstract: Background: Childhood-onset systemic lupus erythematosus (cSLE) is a complex multisystem autoimmune disease often associated with pain, fatigue, and mood-related disturbances. cSLE is associated with increased disease severity and higher rates of ... ...

    Abstract Background: Childhood-onset systemic lupus erythematosus (cSLE) is a complex multisystem autoimmune disease often associated with pain, fatigue, and mood-related disturbances. cSLE is associated with increased disease severity and higher rates of mortality as compared to adult onset SLE. Therefore, a multi-faceted approach to care, including the use of non-pharmacologic therapies, is essential to ensure optimal patient outcomes. The use of non-pharmacologic therapies as adjunctive treatments has been shown to be beneficial in adults with SLE, yet, their use and effect is less well understood in cSLE. This is the first systematic review to explore the use and quality of evidence of non-pharmacologic approaches to treat cSLE.
    Methods: A literature review was performed using PRISMA guidelines. Studies until March 2021 with participants diagnosed with cSLE were included. The quality of the evidence was graded via OCEBM levels of evidence guidelines and bias assessed using Cochrane guidelines. Completed clinical trials (via clinicaltrials.gov) were also searched to identify unpublished results.
    Results: Eleven published studies consisting of 1152 patients met inclusion criteria for this review, as well as three additional studies with unpublished data on clinicaltrial.gov. Of the published trials, four studies used patient education/support, three studies used dietary supplementation, three used forms of psychotherapy (e.g., Cognitive behavioral therapy), and 1 used aerobic exercise to target the following issues: treatment adherence (
    Conclusion: This review identifies several promising non-pharmacologic therapies to use as adjunctive treatments to traditional pharmacologic regimens in health and mental health-related outcomes in patients with cSLE. Future well controlled clinical trials would be beneficial to more rigorously evaluate the effects of non-pharmacologic therapies in pediatric populations.
    MeSH term(s) Adult ; Cardiovascular Diseases ; Child ; Fatigue/etiology ; Fatigue/therapy ; Humans ; Lupus Erythematosus, Systemic/complications ; Lupus Erythematosus, Systemic/diagnosis ; Lupus Erythematosus, Systemic/therapy ; Pain/etiology ; Quality of Life
    Language English
    Publishing date 2022-04-20
    Publishing country England
    Document type Journal Article ; Systematic Review
    ZDB-ID 1154407-7
    ISSN 1477-0962 ; 0961-2033
    ISSN (online) 1477-0962
    ISSN 0961-2033
    DOI 10.1177/09612033221094704
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Long-read Pore-C shows the 3D structure of the cattle genome

    Nguyen, Loan T. / Jung, Hyungtaek / Ma, Jun / Andersen, Stacey / Ross, Elizabeth

    Animal Production Science. 2023, v. 63, no. 11 p.972-982

    2023  

    Abstract: Context Recent advances in molecular technology have allowed us to examine the cattle genome with an accuracy never before possible. Genetic variations, both small and large, as well as the transcriptional landscape of the bovine genome, have both been ... ...

    Abstract Context Recent advances in molecular technology have allowed us to examine the cattle genome with an accuracy never before possible. Genetic variations, both small and large, as well as the transcriptional landscape of the bovine genome, have both been explored in many studies. However, the topological configuration of the genome has not been extensively investigated, largely due to the cost of the assays required. Such assays can both identify topologically associated domains and be used for genome scaffolding. Aims This study aimed to implement a chromatin conformation capture together with long-read nanopore sequencing (Pore-C) pipeline for scaffolding a draft assembly and identifying topologically associating domains (TADs) of a Bos indicus Brahman cow. Methods Genomic DNA from a liver sample was first cross-linked to proteins, preserving the spatial proximity of loci. Restriction digestion and proximity ligation were then used to join cross-linked fragments, followed by nucleic isolation. The Pore-C DNA extracts were then prepped and sequenced on a PromethION device. Two genome assemblies were used to analyse the data, namely, one generated from sequencing of the same Brahman cow, and the other is the ARS-UCD1.2 Bos taurus assembly. The Pore-C snakemake pipeline was used to map, assign bins and scaffold the draft and current annotated bovine assemblies. The contact matrices were then used to identify TADs. Key results The study scaffolded a chromosome-level Bos indicus assembly representing 30 chromosomes. The scaffolded assembly showed a total of 215 contigs (2.6Gbp) with N50 of 44.8Mb. The maximum contig length was 156.8Mb. The GC content of the scaffold assembly is 41±0.02%. Over 50% of mapped chimeric reads identified for both assemblies had three or more contacts. This is the first experimental study to identify TADs in bovine species. In total, 3036 and 3094 TADs across 30 chromosomes were identified for input Brahman and ARS-UCD1.2 assemblies respectively. Conclusions The Pore-C pipeline presented herein will be a valuable approach to scaffold draft assemblies for agricultural species and understand the chromatin structure at different scales. Implications The Pore-C approach will open a new era of 3D genome-organisation studies across agriculture species.
    Keywords Brahman ; DNA ; animal production ; chromatin ; cows ; crosslinking ; genome ; liver ; nanopores ; topology ; transcription (genetics) ; zebu ; 3D chromatin conformation capture ; Bos indicus ; Bos taurus ; Brahman cattle ; multi-way contacts ; nanopore sequencing technologies ; Pore-C ; topologically associating domains</kwd>
    Language English
    Size p. 972-982.
    Publishing place CSIRO Publishing
    Document type Article ; Online
    ZDB-ID 2472524-9
    ISSN 1836-5787 ; 1836-0939
    ISSN (online) 1836-5787
    ISSN 1836-0939
    DOI 10.1071/AN22479
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Vocational Tests for Mental Defectives.

    Ross, Elizabeth L S

    Studies in mental inefficiency

    2017  Volume 2, Issue 1, Page(s) 1–6

    Language English
    Publishing date 2017-09-14
    Publishing country England
    Document type Journal Article
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Multi-breed genomic evaluation for tropical beef cattle when no pedigree information is available.

    Hayes, Ben J / Copley, James / Dodd, Elsie / Ross, Elizabeth M / Speight, Shannon / Fordyce, Geoffry

    Genetics, selection, evolution : GSE

    2023  Volume 55, Issue 1, Page(s) 71

    Abstract: Background: It has been challenging to implement genomic selection in multi-breed tropical beef cattle populations. If commercial (often crossbred) animals could be used in the reference population for these genomic evaluations, this could allow for ... ...

    Abstract Background: It has been challenging to implement genomic selection in multi-breed tropical beef cattle populations. If commercial (often crossbred) animals could be used in the reference population for these genomic evaluations, this could allow for very large reference populations. In tropical beef systems, such animals often have no pedigree information. Here we investigate potential models for such data, using marker heterozygosity (to model heterosis) and breed composition derived from genetic markers, as covariates in the model. Models treated breed effects as either fixed or random, and included genomic best linear unbiased prediction (GBLUP) and BayesR. A tropically-adapted beef cattle dataset of 29,391 purebred, crossbred and composite commercial animals was used to evaluate the models.
    Results: Treating breed effects as random, in an approach analogous to genetic groups allowed partitioning of the genetic variance into within-breed and across breed-components (even with a large number of breeds), and estimation of within-breed and across-breed genomic estimated breeding values (GEBV). We demonstrate that moderately-accurate (0.30-0.43) GEBV can be calculated using these models. Treating breed effects as random gave more accurate GEBV than treating breed as fixed. A simple GBLUP model where no breed effects were fitted gave the same accuracy (and correlations of GEBV very close to 1) as a model where GEBV for within-breed and the GEBV for (random) across-breed effects were included. When GEBV were predicted for herds with no data in the reference population, BayesR resulted in the highest accuracy, with 3% accuracy improvement averaged across traits, especially when the validation population was less related to the reference population. Estimates of heterosis from our models were in line with previous estimates from beef cattle. A method for estimating the number of effective breed comparisons for each breed combination accumulated across contemporary groups is presented.
    Conclusions: When no pedigree is available, breed composition and heterosis for inclusion in multi-breed genomic evaluation can be estimated from genotypes. When GEBV were predicted for herds with no data in the reference population, BayesR resulted in the highest accuracy.
    MeSH term(s) Animals ; Cattle/genetics ; Polymorphism, Single Nucleotide ; Genome ; Genomics/methods ; Genotype ; Phenotype ; Models, Genetic
    Language English
    Publishing date 2023-10-16
    Publishing country France
    Document type Journal Article
    ZDB-ID 1005838-2
    ISSN 1297-9686 ; 0754-0264 ; 0999-193X
    ISSN (online) 1297-9686
    ISSN 0754-0264 ; 0999-193X
    DOI 10.1186/s12711-023-00847-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Regional Anesthesia Use in Pediatric Burn Surgery: A Descriptive Retrospective Series.

    Richman, Michael / Berman, Jeffrey M / Ross, Elizabeth M

    Cureus

    2021  Volume 13, Issue 10, Page(s) e19063

    Abstract: Pediatric burns are a common and often devastating injury. Treatment, reconstruction, and rehabilitation are painful experiences. For some, the experience triggers post-traumatic stress disorder and/or a chronic pain syndrome. Given the role pain plays ... ...

    Abstract Pediatric burns are a common and often devastating injury. Treatment, reconstruction, and rehabilitation are painful experiences. For some, the experience triggers post-traumatic stress disorder and/or a chronic pain syndrome. Given the role pain plays as a major secondary disease, it must be addressed to achieve optimal healing. Regional anesthesia has been used extensively to manage postoperative pain and reduce the need for opioids following other surgical procedures in children. However, regional anesthesia is not widely used in pediatric burn care. We present a descriptive, retrospective case series of 15 pediatric burn patients who received regional anesthesia as part of their intra-operative pain management. In our patient population, we saw low levels of anesthetic as well as opioid usage with well-controlled pain. In this cohort, 93% of patients scored a 0/10 on the Face, Legs, Activity, Cry and Consolability (FLACC) scale for pain by post-anesthesia care unit (PACU) discharge, with an average PACU stay of 70 minutes. Thirty-three percent of patients received no opioids, with the average opioid dose being 0.06mg/kg morphine equivalents. This case series serves to make clinicians aware of the feasibility of use and benefits of regional anesthesia in pediatric burn patients requiring operative repair.
    Language English
    Publishing date 2021-10-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.19063
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Voice outcomes after transoral laser surgery for T1b squamous cell carcinoma of the glottis: Our experience in 20 patients over 12 years.

    Jones, Heidi / Kershaw, Elizabeth / Ross, Elizabeth / Jose, Jemy

    Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery

    2021  Volume 46, Issue 3, Page(s) 679–681

    MeSH term(s) Aged ; Aged, 80 and over ; Carcinoma, Squamous Cell/pathology ; Carcinoma, Squamous Cell/surgery ; Female ; Glottis/pathology ; Glottis/surgery ; Humans ; Laryngeal Neoplasms/pathology ; Laryngeal Neoplasms/surgery ; Laser Therapy ; Male ; Middle Aged ; Neoplasm Staging ; Retrospective Studies ; Voice Quality
    Language English
    Publishing date 2021-02-10
    Publishing country England
    Document type Letter
    ZDB-ID 2205891-6
    ISSN 1749-4486 ; 1749-4478 ; 0307-7772 ; 1365-2273
    ISSN (online) 1749-4486
    ISSN 1749-4478 ; 0307-7772 ; 1365-2273
    DOI 10.1111/coa.13715
    Database MEDical Literature Analysis and Retrieval System OnLINE

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