Artikel ; Online: Functionally impaired antibody response to BNT162b2 booster vaccination in CVID IgG responders.
The Journal of allergy and clinical immunology
2022 Band 151, Heft 4, Seite(n) 922–925
Abstract: Background: Although previous studies described the production of IgG antibodies in a subgroup of patients with common variable immunodeficiency (CVID) following messenger RNA vaccinations with BNT162b2 severe acute respiratory syndrome coronavirus 2 ( ... ...
| Abstract | Background: Although previous studies described the production of IgG antibodies in a subgroup of patients with common variable immunodeficiency (CVID) following messenger RNA vaccinations with BNT162b2 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (CVID responders), the functionality of these antibodies in terms of avidity as measured by the dissociation rate constant (k Objective: We sought to analyze in CVID responders and healthy individuals, the avidity of anti-SARS-CoV-2 serum antibodies and their neutralization capacity as measured by surrogate virus-neutralizing antibodies in addition to IgG-, IgM-, and IgA-antibody levels and the response of circulating (peripheral blood) follicular T-helper cells after a third vaccination with BNT162b2 SARS-CoV-2 messenger RNA vaccine. Methods: Binding IgG, IgA, and IgM serum levels were analyzed by ELISA in patients with CVID responding to the primary vaccination (CVID responders, n = 10) and healthy controls (n = 41). The binding avidity of anti-spike antibodies was investigated using biolayer interferometry in combination with biotin-labeled receptor-binding-domain of SARS-CoV-2 spike protein and streptavidin-labeled sensors. Antigen-specific recall T-cell responses were assessed by measuring activation-induced markers by flow cytometry. Results: After the third vaccination with BNT162b2, IgG-, IgM-, and IgA-antibody levels, surrogate virus-neutralizing antibody levels, and antibody avidity were lower in CVID responders than in healthy controls. In contrast, anti-SARS-CoV-2 spike protein avidity was comparable in CVID responders and healthy individuals following primary vaccination. Follicular T-helper cell response to booster vaccination in CVID responders was significantly reduced when compared with that in healthy individuals. Conclusions: Impaired affinity maturation during booster response provides new insight into CVID pathophysiology. |
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| Mesh-Begriff(e) | Humans ; BNT162 Vaccine ; Common Variable Immunodeficiency ; Antibody Formation ; COVID-19/prevention & control ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; COVID-19 Vaccines ; Antibodies, Blocking ; Antibodies, Viral ; Immunoglobulin A ; Immunoglobulin M |
| Chemische Substanzen | BNT162 Vaccine ; Spike Glycoprotein, Coronavirus ; COVID-19 Vaccines ; Antibodies, Blocking ; Antibodies, Viral ; Immunoglobulin A ; Immunoglobulin M ; spike protein, SARS-CoV-2 |
| Sprache | Englisch |
| Erscheinungsdatum | 2022-12-02 |
| Erscheinungsland | United States |
| Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 121011-7 |
| ISSN | 1097-6825 ; 1085-8725 ; 0091-6749 |
| ISSN (online) | 1097-6825 ; 1085-8725 |
| ISSN | 0091-6749 |
| DOI | 10.1016/j.jaci.2022.11.013 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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