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  1. Book ; Online: Nanobody

    Rothbauer, Ulrich / Chames, Patrick

    2021  

    Keywords Medicine ; Bacillus anthracis ; immunoassay ; single-domain antibody ; genetic fusion ; Beta galactosidase ; P-type ATPase ; nanobody ; llama ; Zinc-transport ; Zinc-transporting P-ATPase ; ZntA ; TNF ; fluorescent ; nanobodies ; sensor ; anti-cytokine therapy ; autoimmune disease ; Western equine encephalitis virus ; MagPlex ; toxin ; antibody ; camelid ; vaccine ; biodefense ; hydrogen exchange-mass spectrometry ; virus ; formatting ; Fc-domain ; half-life ; ischemia ; stroke ; MCAO ; single domain antibodies ; phage display ; intrabody ; intracellular antibody ; GTPase RHO ; BRET ; RAS ; chromobodies ; live-cell imaging ; compound screening ; cellular models ; single-domain antibody fragments ; molecular imaging ; molecular therapy ; nuclear imaging ; targeted fluorescence imaging ; intraoperative imaging ; Nanobody ; Single Domain Antibody ; Cancer ; Immunotherapy ; Imaging ; influenza ; influenza B virus ; hemagglutinin ; single domain antibody ; NanobodyTM ; yeast display ; epitope mapping ; GFP ; C. elegans ; development ; drosophila ; zebrafish ; targeted photodynamic therapy ; hepatocyte growth factor receptor ; HGFR ; c-Met ; Met ; VHH ; photosensitizer ; single-domain antibodies ; neurodegenerative diseases ; brain imaging ; blood-brain barrier ; delivery ; Aids ; HIV ; Llama Antibodies ; bi-specific VHH ; pepscan ; competition studies ; co-crystallisation ; n/a
    Size 1 electronic resource (300 pages)
    Publisher MDPI - Multidisciplinary Digital Publishing Institute
    Publishing place Basel, Switzerland
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021044013
    ISBN 9783036503790 ; 303650379X
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: Speed up to find the right ones: rapid discovery of functional nanobodies.

    Rothbauer, Ulrich

    Nature structural & molecular biology

    2018  Volume 25, Issue 3, Page(s) 199–201

    MeSH term(s) Animals ; Peptide Library ; Receptors, G-Protein-Coupled/immunology ; Single-Domain Antibodies/immunology
    Chemical Substances Peptide Library ; Receptors, G-Protein-Coupled ; Single-Domain Antibodies
    Language English
    Publishing date 2018-02-07
    Publishing country United States
    Document type News
    ZDB-ID 2126708-X
    ISSN 1545-9985 ; 1545-9993
    ISSN (online) 1545-9985
    ISSN 1545-9993
    DOI 10.1038/s41594-018-0038-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4101: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 56: Show issues

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  3. Article ; Online: Peptide-Tag Specific Nanobodies for Studying Proteins in Live Cells.

    Fagbadebo, Funmilayo O / Rothbauer, Ulrich

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2446, Page(s) 555–579

    Abstract: Single-domain antibodies such as nanobodies (Nbs) have substantially expanded the possibilities of advanced cellular imaging. In comparison to conventional antibodies, Nbs are characterized by small size, high stability, and solubility in many ... ...

    Abstract Single-domain antibodies such as nanobodies (Nbs) have substantially expanded the possibilities of advanced cellular imaging. In comparison to conventional antibodies, Nbs are characterized by small size, high stability, and solubility in many environments, including the cytoplasm. Nbs can be efficiently functionalized or modified according to the needs of the imaging approach. Target-specific Nbs can be easily converted into genetically encoded fluorescently labeled intrabodies, also known as chromobodies (CBs), which represent powerful tools to study the dynamics of different proteins of interest within living cells. In this context, CBs specific for a short peptide epitope provide a versatile alternative to bypass the limitations observed with larger fluorescent protein fusions and can be readily used to visualize and monitor peptide-tagged proteins for which specific Nbs are not available. Here, we present our novel detection system comprising a 15 amino acid peptide-tag (PepTag) in combination with a peptide-tag specific CB (PepCB). We provide protocols for adding the PepTag to different proteins of interest, reformatting the peptide-specific Nb (PepNb) into a CB for expression in mammalian cells, and establishment of stable cell lines expressing the PepCB for protein interaction assays and compound screenings.
    MeSH term(s) Animals ; Antibodies/metabolism ; Cytoplasm/metabolism ; Epitopes/metabolism ; Peptides/metabolism ; Single-Domain Antibodies/chemistry
    Chemical Substances Antibodies ; Epitopes ; Peptides ; Single-Domain Antibodies
    Language English
    Publishing date 2022-02-14
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2075-5_29
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: 30 years of nanobodies - an ongoing success story of small binders in biological research.

    Frecot, Desiree I / Froehlich, Theresa / Rothbauer, Ulrich

    Journal of cell science

    2023  Volume 136, Issue 21

    Abstract: A milestone in the field of recombinant binding molecules was achieved 30 years ago with the discovery of single-domain antibodies from which antigen-binding variable domains, better known as nanobodies (Nbs), can be derived. Being only one tenth the ... ...

    Abstract A milestone in the field of recombinant binding molecules was achieved 30 years ago with the discovery of single-domain antibodies from which antigen-binding variable domains, better known as nanobodies (Nbs), can be derived. Being only one tenth the size of conventional antibodies, Nbs feature high affinity and specificity, while being highly stable and soluble. In addition, they display accessibility to cryptic sites, low off-target accumulation and deep tissue penetration. Efficient selection methods, such as (semi-)synthetic/naïve or immunized cDNA libraries and display technologies, have facilitated the isolation of Nbs against diverse targets, and their single-gene format enables easy functionalization and high-yield production. This Review highlights recent advances in Nb applications in various areas of biological research, including structural biology, proteomics and high-resolution and in vivo imaging. In addition, we provide insights into intracellular applications of Nbs, such as live-cell imaging, biosensors and targeted protein degradation.
    MeSH term(s) Single-Domain Antibodies/metabolism
    Chemical Substances Single-Domain Antibodies
    Language English
    Publishing date 2023-11-08
    Publishing country England
    Document type Review ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2993-2
    ISSN 1477-9137 ; 0021-9533
    ISSN (online) 1477-9137
    ISSN 0021-9533
    DOI 10.1242/jcs.261395
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1200: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 14: Show issues

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  5. Article ; Online: Nanobodies - Little helpers unravelling intracellular signaling.

    Wagner, Teresa R / Rothbauer, Ulrich

    Free radical biology & medicine

    2021  Volume 176, Page(s) 46–61

    Abstract: The identification of diagnostic and therapeutic targets requires a comprehensive understanding of cellular processes, for which advanced technologies in biomedical research are needed. The emergence of nanobodies (Nbs) derived from antibody fragments of ...

    Abstract The identification of diagnostic and therapeutic targets requires a comprehensive understanding of cellular processes, for which advanced technologies in biomedical research are needed. The emergence of nanobodies (Nbs) derived from antibody fragments of camelid heavy chain-only antibodies as intracellular research tools offers new possibilities to study and modulate target antigens in living cells. Here we summarize this rapidly changing field, beginning with a brief introduction of Nbs, followed by an overview of how target-specific Nbs can be generated, and introduce the selection of intrabodies as research tools. Intrabodies, by definition, are intracellular functional Nbs that target ectopic or endogenous intracellular antigens within living cells. Such binders can be applied in various formats, e.g. as chromobodies for live cell microscopy or as biosensors to decipher complex intracellular signaling pathways. In addition, protein knockouts can be achieved by target-specific Nbs, while modulating Nbs have the potential as future therapeutics. The development of fine-tunable and switchable Nb-based systems that simultaneously provide spatial and temporal control has recently taken the application of these binders to the next level.
    MeSH term(s) Antibodies ; Antigens ; Single-Domain Antibodies
    Chemical Substances Antibodies ; Antigens ; Single-Domain Antibodies
    Language English
    Publishing date 2021-09-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 807032-5
    ISSN 1873-4596 ; 0891-5849
    ISSN (online) 1873-4596
    ISSN 0891-5849
    DOI 10.1016/j.freeradbiomed.2021.09.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2109: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Special Issue: Nanobody.

    Chames, Patrick / Rothbauer, Ulrich

    Antibodies (Basel, Switzerland)

    2020  Volume 9, Issue 1

    Abstract: Since their first description in 1993 [1], single-domain antibody fragments derived from heavy-chain-only antibodies of camelids have received increasing attention as highly versatile binding molecules in the fields of biotechnology and medicine [ ... ]. ...

    Abstract Since their first description in 1993 [1], single-domain antibody fragments derived from heavy-chain-only antibodies of camelids have received increasing attention as highly versatile binding molecules in the fields of biotechnology and medicine [...].
    Language English
    Publishing date 2020-03-06
    Publishing country Switzerland
    Document type Editorial
    ISSN 2073-4468
    ISSN (online) 2073-4468
    DOI 10.3390/antib9010006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Nanobodies Right in the Middle: Intrabodies as Toolbox to Visualize and Modulate Antigens in the Living Cell.

    Wagner, Teresa R / Rothbauer, Ulrich

    Biomolecules

    2020  Volume 10, Issue 12

    Abstract: In biomedical research, there is an ongoing demand for new technologies to elucidate disease mechanisms and develop novel therapeutics. This requires comprehensive understanding of cellular processes and their pathophysiology based on reliable ... ...

    Abstract In biomedical research, there is an ongoing demand for new technologies to elucidate disease mechanisms and develop novel therapeutics. This requires comprehensive understanding of cellular processes and their pathophysiology based on reliable information on abundance, localization, post-translational modifications and dynamic interactions of cellular components. Traceable intracellular binding molecules provide new opportunities for real-time cellular diagnostics. Most prominently, intrabodies derived from antibody fragments of heavy-chain only antibodies of camelids (nanobodies) have emerged as highly versatile and attractive probes to study and manipulate antigens within the context of living cells. In this review, we provide an overview on the selection, delivery and usage of intrabodies to visualize and monitor cellular antigens in living cells and organisms. Additionally, we summarize recent advances in the development of intrabodies as cellular biosensors and their application to manipulate disease-related cellular processes. Finally, we highlight switchable intrabodies, which open entirely new possibilities for real-time cell-based diagnostics including live-cell imaging, target validation and generation of precisely controllable binding reagents for future therapeutic applications.
    MeSH term(s) Animals ; Antibodies/genetics ; Antigens/chemistry ; Biosensing Techniques ; Camelus ; Cytoplasm/metabolism ; Green Fluorescent Proteins/metabolism ; Humans ; Medical Oncology ; Nervous System Diseases ; Peptide Library ; Protein Binding ; Protein Processing, Post-Translational ; Single-Domain Antibodies/chemistry
    Chemical Substances Antibodies ; Antigens ; Peptide Library ; Single-Domain Antibodies ; Green Fluorescent Proteins (147336-22-9)
    Language English
    Publishing date 2020-12-21
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom10121701
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Book ; Online ; Thesis: A microplate co-culture assay comprising patient-derived 3D microtumors

    Bodenhöfer, Mona [Verfasser] / Rothbauer, Ulrich [Akademischer Betreuer]

    2023  

    Author's details Mona Bodenhöfer ; Akademischer Betreuer: Ulrich Rothbauer
    Keywords Naturwissenschaften ; Science
    Subject code sg500
    Language English
    Publisher Universitätsbibliothek Tübingen
    Publishing place Tübingen
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  9. Book ; Online ; Thesis: Entwicklung und Validierung multiplexer Assays zur Analyse der humoralen Immunantwort bei einer Borrelien-Infektion

    Häring, Julia [Verfasser] / Rothbauer, Ulrich [Akademischer Betreuer]

    2023  

    Author's details Julia Christina Häring ; Betreuer: Ulrich Rothbauer
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language German
    Publisher Universitätsbibliothek Tübingen
    Publishing place Tübingen
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  10. Book ; Online ; Thesis: Serological Analysis to Accompany Clinical Research, Vaccination and Evolution of the COVID-19 Pandemic

    Becker, Matthias [Verfasser] / Rothbauer, Ulrich [Akademischer Betreuer]

    2023  

    Author's details Matthias Becker ; Betreuer: Ulrich Rothbauer
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universitätsbibliothek Tübingen
    Publishing place Tübingen
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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