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Article ; Online: BCG infection dose guides dendritic cell migration and T cell priming in the draining lymph node.

Krmeská, Veronika / Shen, Lei / Nylén, Susanne / Wowk, Pryscilla Fanini / Rothfuchs, Antonio Gigliotti

2023 Volume 99, Issue 3, Page(s) e13342

Abstract: In contrast to delayed-type hypersensitivity (DTH) and other hallmark reactions of cell-mediated immunity that correlate with vaccine-mediated protection against Mycobacterium tuberculosis, the contribution of vaccine dose on responses that emerge early ... ...

Abstract	In contrast to delayed-type hypersensitivity (DTH) and other hallmark reactions of cell-mediated immunity that correlate with vaccine-mediated protection against Mycobacterium tuberculosis, the contribution of vaccine dose on responses that emerge early after infection in the skin with Bacille Calmette-Guérin (BCG) is not well understood. We used a mouse model of BCG skin infection to study the effect of BCG dose on the relocation of skin Dendritic cells (DCs) to draining lymph node (DLN). Mycobacterium antigen 85B-specific CD4
MeSH term(s)	Mice ; Animals ; Guinea Pigs ; T-Lymphocytes ; BCG Vaccine ; Immunity, Cellular ; Langerhans Cells ; Lymph Nodes
Chemical Substances	BCG Vaccine
Language	English
Publishing date	2023-11-21
Publishing country	England
Document type	Journal Article
ZDB-ID	120476-2
ISSN	1365-3083 ; 0300-9475
ISSN (online)	1365-3083
ISSN	0300-9475
DOI	10.1111/sji.13342
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Article ; Online: Cyclooxygenase-Derived Prostaglandin E

Krmeská, Veronika / Aggio, Juliana Bernardi / Nylén, Susanne / Wowk, Pryscilla Fanini / Rothfuchs, Antonio Gigliotti

Journal of immunology (Baltimore, Md. : 1950)

2022 Volume 208, Issue 11, Page(s) 2549–2557

Abstract: Inoculation ... ...

Abstract	Inoculation of
MeSH term(s)	Animals ; BCG Vaccine ; Cyclooxygenase 2/metabolism ; Dendritic Cells ; Dinoprostone/pharmacology ; Interleukin-1/metabolism ; Langerhans Cells ; Lymph Nodes ; Mice ; Mycobacterium bovis ; Receptors, Prostaglandin E, EP4 Subtype/metabolism
Chemical Substances	BCG Vaccine ; Interleukin-1 ; Receptors, Prostaglandin E, EP4 Subtype ; Cyclooxygenase 2 (EC 1.14.99.1) ; Dinoprostone (K7Q1JQR04M)
Language	English
Publishing date	2022-05-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.2100981
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Article ; Online: Vaccinia Virus Infection Inhibits Skin Dendritic Cell Migration to the Draining Lymph Node.

Aggio, Juliana Bernardi / Krmeská, Veronika / Ferguson, Brian J / Wowk, Pryscilla Fanini / Rothfuchs, Antonio Gigliotti

Journal of immunology (Baltimore, Md. : 1950)

2021 Volume 206, Issue 4, Page(s) 776–784

Abstract: There is a paucity of information on dendritic cell (DC) responses to vaccinia virus (VACV), including the traffic of DCs to the draining lymph node (dLN). In this study, using a mouse model of infection, we studied skin DC migration in response to VACV ... ...

Abstract	There is a paucity of information on dendritic cell (DC) responses to vaccinia virus (VACV), including the traffic of DCs to the draining lymph node (dLN). In this study, using a mouse model of infection, we studied skin DC migration in response to VACV and compared it with the tuberculosis vaccine
MeSH term(s)	Animals ; CD4-Positive T-Lymphocytes/immunology ; Cell Movement/genetics ; Cell Movement/immunology ; Dendritic Cells/immunology ; Lymph Nodes/immunology ; Mice ; Mice, Knockout ; Mycobacterium bovis/immunology ; Skin/immunology ; Vaccinia/genetics ; Vaccinia/immunology ; Vaccinia virus/genetics ; Vaccinia virus/immunology
Language	English
Publishing date	2021-01-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.2000928
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Article ; Online: Operative and Technical Modifications to the Coriolis® µ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling.

Rufino de Sousa, Nuno / Shen, Lei / Silcott, David / Call, Charles J / Rothfuchs, Antonio Gigliotti

Annals of work exposures and health

2020 Volume 64, Issue 8, Page(s) 852–865

Abstract: Detecting infectious aerosols is central for gauging and countering airborne threats. In this regard, the Coriolis® µ cyclonic air sampler is a practical, commercial collector that can be used with various analysis methods to monitor pathogens in air. ... ...

Abstract	Detecting infectious aerosols is central for gauging and countering airborne threats. In this regard, the Coriolis® µ cyclonic air sampler is a practical, commercial collector that can be used with various analysis methods to monitor pathogens in air. However, information on how to operate this unit under optimal sampling and biosafety conditions is limited. We investigated Coriolis performance in aerosol dispersal experiments with polystyrene microspheres and Bacillus globigii spores. We report inconsistent sample recovery from the collector cone due to loss of material when sampling continuously for more than 30 min. Introducing a new collector cone every 10 min improved this shortcoming. Moreover, we found that several surfaces on the device become contaminated during sampling. Adapting a high efficiency particulate air-filter system to the Coriolis prevented contamination without altering collection efficiency or tactical deployment. A Coriolis modified with these operative and technical improvements was used to collect aerosols carrying microspheres released inside a Biosafety Level-3 laboratory during simulations of microbiological spills and aerosol dispersals. In summary, we provide operative and technical solutions to the Coriolis that optimize microbiological air sampling and improve biosafety.
MeSH term(s)	Aerosols/analysis ; Air Pollutants ; Bacillus ; Containment of Biohazards ; Dust ; Humans ; Occupational Exposure/analysis
Chemical Substances	Aerosols ; Air Pollutants ; Dust
Keywords	covid19
Language	English
Publishing date	2020-05-29
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2885096-8
ISSN	2398-7316 ; 2398-7308
ISSN (online)	2398-7316
ISSN	2398-7308
DOI	10.1093/annweh/wxaa053
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Article ; Online: A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin.

Bollampalli, Vishnu Priya / Nylén, Susanne / Rothfuchs, Antonio Gigliotti

Journal of visualized experiments : JoVE

2016 , Issue 116

Abstract: Dendritic cells (DCs) are important for initiating immune responses, in part through their ability to acquire and shuttle antigen to the draining lymph node (DLN). The mobilization of DCs to the DLN is complex and remains to be fully elucidated during ... ...

Abstract	Dendritic cells (DCs) are important for initiating immune responses, in part through their ability to acquire and shuttle antigen to the draining lymph node (DLN). The mobilization of DCs to the DLN is complex and remains to be fully elucidated during infection. Herein described is the use of an innovative, simple assay that relies on the fluorochrome 5- and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) to track the migration of DCs during footpad infection with Mycobacterium bovis Bacille Calmette-Guérin (BCG) in C57BL/6 mice. This assay enables the characterization of skin DC sub-populations that actively relocate to the draining, popliteal LN in response to BCG. This protocol originates from a BCG model where migratory skin DCs were identified by flow cytometry. The assay is amiable to the study and identification of DCs or other cells that home to the popliteal LN after inoculation of microbes, their metabolites or other inflammatory stimuli in the footpad, and consequently to study factors that regulate the migration of these cells.
MeSH term(s)	Animals ; Cattle ; Dendritic Cells ; Flow Cytometry ; Langerhans Cells ; Lymph Nodes ; Mice ; Mice, Inbred C57BL ; Mycobacterium bovis ; Tuberculosis, Bovine
Language	English
Publishing date	2016-10-09
Publishing country	United States
Document type	Journal Article ; Video-Audio Media
ISSN	1940-087X
ISSN (online)	1940-087X
DOI	10.3791/54620
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Article: A cfse-based assay to study the migration of murine skin dendritic cells into draining lymph nodes during infection with Mycobacterium bovis bacille calmette-guérin

Bollampalli, Vishnu Priya / Nylén, Susanne / Rothfuchs, Antonio Gigliotti

Journal of visualized experiments. 2016 Oct. 09, , no. 116

2016

Abstract	Dendritic cells (DCs) are important for initiating immune responses, in part through their ability to acquire and shuttle antigen to the draining lymph node (DLN). The mobilization of DCs to the DLN is complex and remains to be fully elucidated during infection. Herein described is the use of an innovative, simple assay that relies on the fluorochrome 5- and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) to track the migration of DCs during footpad infection with Mycobacterium bovis Bacille Calmette-Guérin (BCG) in C57BL/6 mice. This assay enables the characterization of skin DC sub-populations that actively relocate to the draining, popliteal LN in response to BCG. This protocol originates from a BCG model where migratory skin DCs were identified by flow cytometry. The assay is amiable to the study and identification of DCs or other cells that home to the popliteal LN after inoculation of microbes, their metabolites or other inflammatory stimuli in the footpad, and consequently to study factors that regulate the migration of these cells.
Keywords	Mycobacterium bovis ; antigens ; dendritic cells ; flow cytometry ; fluorescent dyes ; immune response ; lymph nodes ; metabolites ; mice ; microorganisms ; migratory behavior ; models
Language	English
Dates of publication	2016-1009
Size	p. e54620.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/54620
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Plaque-forming units from air samples: Letter to Editor. Re: Jefferson et al., Indoor Air, 2022.

Rufino de Sousa, Nuno / Steponaviciute, Laura / Margerie, Lucille / Nissen, Karolina / Kjellin, Midori / Reinius, Björn / Salaneck, Erik / Udekwu, Klas I / Rothfuchs, Antonio Gigliotti

Indoor air

2022 Volume 32, Issue 11, Page(s) e13169

MeSH term(s)	Air Pollution, Indoor/analysis ; Air Microbiology ; Air Pollutants/analysis
Chemical Substances	Air Pollutants
Language	English
Publishing date	2022-11-27
Publishing country	England
Document type	Letter ; Research Support, Non-U.S. Gov't ; Comment
ZDB-ID	1081722-0
ISSN	1600-0668 ; 0905-6947
ISSN (online)	1600-0668
ISSN	0905-6947
DOI	10.1111/ina.13169
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Article ; Online: Corrigendum. Re: de Sousa, N.R., et al., 2022. Detection and isolation of airborne SARS-CoV-2 in a hospital setting. Indoor air, 32(3), e13023.

Rufino de Sousa, Nuno / Steponaviciute, Laura / Margerie, Lucille / Nissen, Karolina / Kjellin, Midori / Reinius, Björn / Salaneck, Erik / Udekwu, Klas I / Rothfuchs, Antonio Gigliotti

Indoor air

2022 Volume 32, Issue 8, Page(s) e13085

MeSH term(s)	Air Pollution, Indoor ; COVID-19 ; Hospitals ; Humans ; SARS-CoV-2
Language	English
Publishing date	2022-08-28
Publishing country	England
Document type	Letter ; Published Erratum
ZDB-ID	1081722-0
ISSN	1600-0668 ; 0905-6947
ISSN (online)	1600-0668
ISSN	0905-6947
DOI	10.1111/ina.13085
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Article ; Online: Detection and isolation of airborne SARS-CoV-2 in a hospital setting.

Rufino de Sousa, Nuno / Steponaviciute, Laura / Margerie, Lucille / Nissen, Karolina / Kjellin, Midori / Reinius, Björn / Salaneck, Erik / Udekwu, Klas I / Rothfuchs, Antonio Gigliotti

Indoor air

2022 Volume 32, Issue 3, Page(s) e13023

Abstract: Transmission mechanisms for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are incompletely understood. In particular, aerosol transmission remains unclear, with viral detection in air and demonstration of its infection potential being ... ...

Abstract	Transmission mechanisms for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are incompletely understood. In particular, aerosol transmission remains unclear, with viral detection in air and demonstration of its infection potential being actively investigated. To this end, we employed a novel electrostatic collector to sample air from rooms occupied by COVID-19 patients in a major Swedish hospital. Electrostatic air sampling in conjunction with extraction-free, reverse-transcriptase polymerase chain reaction (hid-RT-PCR) enabled detection of SARS-CoV-2 in air from patient rooms (9/22; 41%) and adjoining anterooms (10/22; 45%). Detection with hid-RT-PCR was concomitant with viral RNA presence on the surface of exhaust ventilation channels in patients and anterooms more than 2 m from the COVID-19 patient. Importantly, it was possible to detect active SARS-CoV-2 particles from room air, with a total of 496 plaque-forming units (PFUs) being isolated, establishing the presence of infectious, airborne SARS-CoV-2 in rooms occupied by COVID-19 patients. Our results support circulation of SARS-CoV-2 via aerosols and urge the revision of existing infection control frameworks to include airborne transmission.
MeSH term(s)	Air Pollution, Indoor ; COVID-19 ; Hospitals ; Humans ; RNA, Viral/analysis ; SARS-CoV-2
Chemical Substances	RNA, Viral
Language	English
Publishing date	2022-03-28
Publishing country	England
Document type	Journal Article
ZDB-ID	1081722-0
ISSN	1600-0668 ; 0905-6947
ISSN (online)	1600-0668
ISSN	0905-6947
DOI	10.1111/ina.13023
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Article ; Online: A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols.

Rufino de Sousa, Nuno / Sandström, Niklas / Shen, Lei / Håkansson, Kathleen / Vezozzo, Rafaella / Udekwu, Klas I / Croda, Julio / Rothfuchs, Antonio Gigliotti

Tuberculosis (Edinburgh, Scotland)

2019 Volume 120, Page(s) 101896

Abstract: Tuberculosis (TB) infects about 25% of the world population and claims more human lives than any other infectious disease. TB is spread by inhalation of aerosols containing viable Mycobacterium tuberculosis expectorated or exhaled by patients with active ...

Abstract	Tuberculosis (TB) infects about 25% of the world population and claims more human lives than any other infectious disease. TB is spread by inhalation of aerosols containing viable Mycobacterium tuberculosis expectorated or exhaled by patients with active pulmonary disease. Air-sampling technology could play an important role in TB control by enabling the detection of airborne M. tuberculosis, but tools that are easy to use and scalable in TB hotspots are lacking. We developed an electrostatic air sampler termed the TB Hotspot DetectOR (THOR) and investigated its performance in laboratory aerosol experiments and in a prison hotspot of TB transmission. We show that THOR collects aerosols carrying microspheres, Bacillus globigii spores and M. bovis BCG, concentrating these microparticles onto a collector piece designed for subsequent detection analysis. The unit was also successfully operated in the complex setting of a prison hotspot, enabling detection of a molecular signature for M. tuberculosis in the cough of inmates. Future deployment of this device may lead to a measurable impact on TB case-finding by screening individuals through the aerosols they generate.
MeSH term(s)	Aerosols ; Air Microbiology ; Bacteriological Techniques ; Cough/microbiology ; DNA, Bacterial/genetics ; Environmental Monitoring ; Humans ; Mycobacterium bovis/genetics ; Mycobacterium bovis/isolation & purification ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/isolation & purification ; Polymerase Chain Reaction ; Prisons ; Static Electricity ; Tuberculosis, Pulmonary/diagnosis ; Tuberculosis, Pulmonary/microbiology ; Tuberculosis, Pulmonary/transmission
Chemical Substances	Aerosols ; DNA, Bacterial
Language	English
Publishing date	2019-12-24
Publishing country	Scotland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2046804-0
ISSN	1873-281X ; 1472-9792
ISSN (online)	1873-281X
ISSN	1472-9792
DOI	10.1016/j.tube.2019.101896
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