Article: Study of a polymerase chain reaction-based method for detection of herpes simplex virus type 1 DNA among Iranian patients with ocular herpetic keratitis infection.
Japanese journal of ophthalmology
2004 Volume 48, Issue 4, Page(s) 328–332
Abstract: Purpose: To study the presence of the herpes simplex virus type 1 (HSV-1) glycoprotein D gene in tear films of Iranian patients with herpetic keratitis.: Methods: Twenty-five tear film and eye swab specimens from 25 herpetic keratitis patients and 10 ...
| Abstract | Purpose: To study the presence of the herpes simplex virus type 1 (HSV-1) glycoprotein D gene in tear films of Iranian patients with herpetic keratitis. Methods: Twenty-five tear film and eye swab specimens from 25 herpetic keratitis patients and 10 specimens from 10 healthy volunteers were collected in the Farabi Eye Hospital, Tehran, Iran. HSV-1 DNA was detected by using the nested polymerase chain reaction (nPCR) method. Viral isolation was done using conventional viral techniques. A monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) was used for confirmation of positive cytopathic effect cell culture. The results of a diagnosis by an ophthalmologist team were compared with those of nPCR. Results: HSV-1 DNA was identified in tear films of 88% (23/25) of suspected herpetic keratitis patients. All healthy controls (100%) had negative PCR results. HSV-1 was isolated in cell culture and confirmed by ELISA in 12% (3/25) of herpetic keratitis patients who had epithelial keratitis. The kappa value showed a high level of agreement between ophthalmologist team diagnosis and the PCR results (kappa = 0.86, P < 0.0001). Conclusions: nPCR is a sensitive, rapid, and powerful tool for detection of HSV-1 DNA in tear films of ocular herpetic keratitis patients and can serve as a supplemental method for diagnosis of herpetic keratitis infection. |
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| MeSH term(s) | Animals ; Antibodies, Viral/analysis ; Cercopithecus aethiops ; Cytopathogenic Effect, Viral ; DNA, Viral/analysis ; Enzyme-Linked Immunosorbent Assay ; Female ; HeLa Cells ; Herpesvirus 1, Human/genetics ; Herpesvirus 1, Human/immunology ; Herpesvirus 1, Human/isolation & purification ; Humans ; Iran ; Keratitis, Herpetic/virology ; Male ; Polymerase Chain Reaction/methods ; Tears/virology ; Vero Cells ; Viral Envelope Proteins/genetics ; Virus Cultivation/methods | |||||
| Chemical Substances | Antibodies, Viral ; DNA, Viral ; Viral Envelope Proteins ; glycoprotein D, Human herpesvirus 1 | |||||
| Language | English | |||||
| Publishing date | 2004-07 | |||||
| Publishing country | Japan | |||||
| Document type | Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 82032-5 | |||||
| ISSN | 1613-2246 ; 0021-5155 | |||||
| ISSN (online) | 1613-2246 | |||||
| ISSN | 0021-5155 | |||||
| DOI | 10.1007/s10384-004-0081-z | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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