LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU="Rovira-Clavé, Xavier"
  2. AU="Den Boer, Monique L"
  3. AU="Potts, T."
  4. AU="Cifuentes-Diaz, Carmen"
  5. AU="Alvim, Ricardo G"
  6. AU="Barron II, Joseph C"
  7. AU="Godin, Shea-Lee"
  8. AU="Leng, Chengcai"
  9. AU="Hyslop, Brian W"
  10. AU="Suzanne Fischer"
  11. AU="Aboelata, Noha"
  12. AU="Chiang, Sarah N"
  13. AU="Wessel, Kristin M"
  14. AU="Wilson, Jenna M"
  15. AU="Goines, Paula"
  16. AU=Ippolito Mariachiara AU=Ippolito Mariachiara
  17. AU="Jose Chauca"
  18. AU="Asih, Puji B S"
  19. AU="Dsane-Selby, Lydia"
  20. AU="Tolossa, Tadesse"
  21. AU="Erdal Bedir"

Suchergebnis

Treffer 1 - 10 von insgesamt 16

Suchoptionen

  1. Artikel: A Spatial Multi-Modal Dissection of Host-Microbiome Interactions within the Colitis Tissue Microenvironment.

    Zhu, Bokai / Bai, Yunhao / Yeo, Yao Yu / Lu, Xiaowei / Rovira-Clavé, Xavier / Chen, Han / Yeung, Jason / Gerber, Georg K / Angelo, Mike / Shalek, Alex K / Nolan, Garry P / Jiang, Sizun

    bioRxiv : the preprint server for biology

    2024  

    Abstract: The intricate and dynamic interactions between the host immune system and its microbiome constituents undergo dynamic shifts in response to perturbations to the intestinal tissue environment. Our ability to study these events on the systems level is ... ...

    Abstract The intricate and dynamic interactions between the host immune system and its microbiome constituents undergo dynamic shifts in response to perturbations to the intestinal tissue environment. Our ability to study these events on the systems level is significantly limited by
    Sprache Englisch
    Erscheinungsdatum 2024-03-06
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2024.03.04.583400
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  2. Artikel ; Online: ATM meets ERK5.

    Angulo-Ibáñez, Maria / Rovira-Clavé, Xavier / Espel, Enric

    Aging

    2017  Band 9, Heft 2, Seite(n) 299–300

    Mesh-Begriff(e) Animals ; Ataxia Telangiectasia Mutated Proteins/metabolism ; Humans ; Mitogen-Activated Protein Kinase 7/metabolism
    Chemische Substanzen Ataxia Telangiectasia Mutated Proteins (EC 2.7.11.1) ; Mitogen-Activated Protein Kinase 7 (EC 2.7.11.24)
    Sprache Englisch
    Erscheinungsdatum 2017-03-14
    Erscheinungsland United States
    Dokumenttyp Editorial
    ISSN 1945-4589
    ISSN (online) 1945-4589
    DOI 10.18632/aging.101189
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  3. Artikel ; Online: T cell-mediated curation and restructuring of tumor tissue coordinates an effective immune response.

    Hickey, John W / Haist, Maximillian / Horowitz, Nina / Caraccio, Chiara / Tan, Yuqi / Rech, Andrew J / Baertsch, Marc-Andrea / Rovira-Clavé, Xavier / Zhu, Bokai / Vazquez, Gustavo / Barlow, Graham / Agmon, Eran / Goltsev, Yury / Sunwoo, John B / Covert, Markus / Nolan, Garry P

    Cell reports

    2023  Band 42, Heft 12, Seite(n) 113494

    Abstract: Antigen-specific T cells traffic to, are influenced by, and create unique cellular microenvironments. Here we characterize these microenvironments over time with multiplexed imaging in a melanoma model of adoptive T cell therapy and human patients with ... ...

    Abstract Antigen-specific T cells traffic to, are influenced by, and create unique cellular microenvironments. Here we characterize these microenvironments over time with multiplexed imaging in a melanoma model of adoptive T cell therapy and human patients with melanoma treated with checkpoint inhibitor therapy. Multicellular neighborhood analysis reveals dynamic immune cell infiltration and inflamed tumor cell neighborhoods associated with CD8
    Mesh-Begriff(e) Humans ; Melanoma/pathology ; CD8-Positive T-Lymphocytes ; Immunotherapy/methods ; Cytokines ; Immunity ; Tumor Microenvironment
    Chemische Substanzen Cytokines
    Sprache Englisch
    Erscheinungsdatum 2023-12-11
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2649101-1
    ISSN 2211-1247 ; 2211-1247
    ISSN (online) 2211-1247
    ISSN 2211-1247
    DOI 10.1016/j.celrep.2023.113494
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  4. Artikel ; Online: Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology.

    Bai, Yunhao / Zhu, Bokai / Oliveria, John-Paul / Cannon, Bryan J / Feyaerts, Dorien / Bosse, Marc / Vijayaragavan, Kausalia / Greenwald, Noah F / Phillips, Darci / Schürch, Christian M / Naik, Samuel M / Ganio, Edward A / Gaudilliere, Brice / Rodig, Scott J / Miller, Michael B / Angelo, Michael / Bendall, Sean C / Rovira-Clavé, Xavier / Nolan, Garry P /
    Jiang, Sizun

    Nature communications

    2023  Band 14, Heft 1, Seite(n) 4013

    Abstract: Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex imaging technologies are limited in resolving subcellular biomolecular features. Expansion Microscopy (ExM) and related techniques physically expand samples for ... ...

    Abstract Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex imaging technologies are limited in resolving subcellular biomolecular features. Expansion Microscopy (ExM) and related techniques physically expand samples for enhanced spatial resolution, but are challenging to be combined with high-plex imaging technologies to enable integrative multiscaled tissue biology insights. Here, we introduce Expand and comPRESS hydrOgels (ExPRESSO), an ExM framework that allows high-plex protein staining, physical expansion, and removal of water, while retaining the lateral tissue expansion. We demonstrate ExPRESSO imaging of archival clinical tissue samples on Multiplexed Ion Beam Imaging and Imaging Mass Cytometry platforms, with detection capabilities of > 40 markers. Application of ExPRESSO on archival human lymphoid and brain tissues resolved tissue architecture at the subcellular level, particularly that of the blood-brain barrier. ExPRESSO hence provides a platform for extending the analysis compatibility of hydrogel-expanded biospecimens to mass spectrometry, with minimal modifications to protocols and instrumentation.
    Mesh-Begriff(e) Humans ; Vacuum ; Microscopy/methods ; Proteins ; Hydrogels/chemistry
    Chemische Substanzen Proteins ; Hydrogels
    Sprache Englisch
    Erscheinungsdatum 2023-07-07
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-39616-w
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  5. Artikel ; Online: Subcellular localization of biomolecules and drug distribution by high-definition ion beam imaging.

    Rovira-Clavé, Xavier / Jiang, Sizun / Bai, Yunhao / Zhu, Bokai / Barlow, Graham / Bhate, Salil / Coskun, Ahmet F / Han, Guojun / Ho, Chin-Min Kimmy / Hitzman, Chuck / Chen, Shih-Yu / Bava, Felice-Alessio / Nolan, Garry P

    Nature communications

    2021  Band 12, Heft 1, Seite(n) 4628

    Abstract: Simultaneous visualization of the relationship between multiple biomolecules and their ligands or small molecules at the nanometer scale in cells will enable greater understanding of how biological processes operate. We present here high-definition ... ...

    Abstract Simultaneous visualization of the relationship between multiple biomolecules and their ligands or small molecules at the nanometer scale in cells will enable greater understanding of how biological processes operate. We present here high-definition multiplex ion beam imaging (HD-MIBI), a secondary ion mass spectrometry approach capable of high-parameter imaging in 3D of targeted biological entities and exogenously added structurally-unmodified small molecules. With this technology, the atomic constituents of the biomolecules themselves can be used in our system as the "tag" and we demonstrate measurements down to ~30 nm lateral resolution. We correlated the subcellular localization of the chemotherapy drug cisplatin simultaneously with five subnuclear structures. Cisplatin was preferentially enriched in nuclear speckles and excluded from closed-chromatin regions, indicative of a role for cisplatin in active regions of chromatin. Unexpectedly, cells surviving multi-drug treatment with cisplatin and the BET inhibitor JQ1 demonstrated near total cisplatin exclusion from the nucleus, suggesting that selective subcellular drug relocalization may modulate resistance to this important chemotherapeutic treatment. Multiplexed high-resolution imaging techniques, such as HD-MIBI, will enable studies of biomolecules and drug distributions in biologically relevant subcellular microenvironments by visualizing the processes themselves in concert, rather than inferring mechanism through surrogate analyses.
    Mesh-Begriff(e) Antineoplastic Agents/metabolism ; Antineoplastic Agents/pharmacokinetics ; Azepines/metabolism ; Azepines/pharmacokinetics ; Cell Line, Tumor ; Cell Nucleus/metabolism ; Cisplatin/metabolism ; Cisplatin/pharmacokinetics ; Cytoplasm/metabolism ; HeLa Cells ; Humans ; Intracellular Space/metabolism ; Jurkat Cells ; Microscopy, Confocal ; Spectrometry, Mass, Secondary Ion/methods ; Triazoles/metabolism ; Triazoles/pharmacokinetics
    Chemische Substanzen (+)-JQ1 compound ; Antineoplastic Agents ; Azepines ; Triazoles ; Cisplatin (Q20Q21Q62J)
    Sprache Englisch
    Erscheinungsdatum 2021-07-30
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-021-24822-1
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  6. Artikel ; Online: Adjacent Cell Marker Lateral Spillover Compensation and Reinforcement for Multiplexed Images.

    Bai, Yunhao / Zhu, Bokai / Rovira-Clave, Xavier / Chen, Han / Markovic, Maxim / Chan, Chi Ngai / Su, Tung-Hung / McIlwain, David R / Estes, Jacob D / Keren, Leeat / Nolan, Garry P / Jiang, Sizun

    Frontiers in immunology

    2021  Band 12, Seite(n) 652631

    Abstract: Multiplex imaging technologies are now routinely capable of measuring more than 40 antibody-labeled parameters in single cells. However, lateral spillage of signals in densely packed tissues presents an obstacle to the assignment of high-dimensional ... ...

    Abstract Multiplex imaging technologies are now routinely capable of measuring more than 40 antibody-labeled parameters in single cells. However, lateral spillage of signals in densely packed tissues presents an obstacle to the assignment of high-dimensional spatial features to individual cells for accurate cell-type annotation. We devised a method to correct for lateral spillage of cell surface markers between adjacent cells termed REinforcement Dynamic Spillover EliminAtion (REDSEA). The use of REDSEA decreased contaminating signals from neighboring cells. It improved the recovery of marker signals across both isotopic (i.e., Multiplexed Ion Beam Imaging) and immunofluorescent (i.e., Cyclic Immunofluorescence) multiplexed images resulting in a marked improvement in cell-type classification.
    Mesh-Begriff(e) Animals ; Biomarkers ; Cell Lineage ; Fluorescent Antibody Technique/methods ; Image Processing, Computer-Assisted ; Molecular Imaging/methods ; Molecular Imaging/standards ; Reproducibility of Results ; Sensitivity and Specificity ; Signal-To-Noise Ratio ; Single-Cell Analysis/methods ; Single-Cell Analysis/standards
    Chemische Substanzen Biomarkers
    Sprache Englisch
    Erscheinungsdatum 2021-07-05
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2021.652631
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  7. Artikel ; Online: The PDZ-binding domain of syndecan-2 inhibits LFA-1 high-affinity conformation.

    Rovira-Clavé, Xavier / Angulo-Ibáñez, Maria / Reina, Manuel / Espel, Enric

    Cellular signalling

    2014  Band 26, Heft 7, Seite(n) 1489–1499

    Abstract: Syndecans are cell membrane proteoglycans that can modulate the activity and dynamics of some growth factor receptors and integrins. Here, we show the down-regulation of integrin lymphocyte function-associated antigen-1 (LFA-1) and inhibition of adhesion ...

    Abstract Syndecans are cell membrane proteoglycans that can modulate the activity and dynamics of some growth factor receptors and integrins. Here, we show the down-regulation of integrin lymphocyte function-associated antigen-1 (LFA-1) and inhibition of adhesion of Jurkat T cells transfected with syndecan-2. The PDZ-binding domain in the cytoplasmic region of syndecan-2 was necessary to block the LFA-1 high-affinity conformation, and to reduce cellular adhesion. A second cytoplasmic motif comprising tyrosines 179 and 191, and serines 187 and 188 contributed also to reduce LFA-1 function and cellular adhesion. Inhibition of the LFA-1 high-affinity conformation by syndecan-2 was independent of the expression of the talin head domain and RhoA, Rac1 and Cdc42 GTPases. These results demonstrate the importance of PDZ-binding domain of syndecan-2 for controlling LFA-1 affinity and cell adhesion.
    Mesh-Begriff(e) B-Lymphocytes/metabolism ; Cell Adhesion/physiology ; Cell Line, Tumor ; Cell Membrane/metabolism ; Cell Movement ; Down-Regulation ; Endothelium/cytology ; Endothelium/metabolism ; GTPase-Activating Proteins/biosynthesis ; Humans ; Jurkat Cells ; Lymphocyte Function-Associated Antigen-1/biosynthesis ; Lymphocyte Function-Associated Antigen-1/metabolism ; PDZ Domains/genetics ; Phosphoproteins/biosynthesis ; Protein Binding ; Syndecan-2/genetics ; Syndecan-2/metabolism ; T-Lymphocytes/metabolism ; Talin/biosynthesis ; Transfection ; rac1 GTP-Binding Protein/biosynthesis ; rhoA GTP-Binding Protein/biosynthesis
    Chemische Substanzen ARHGAP31 protein, human ; GTPase-Activating Proteins ; Lymphocyte Function-Associated Antigen-1 ; Phosphoproteins ; RAC1 protein, human ; SDC2 protein, human ; Talin ; Syndecan-2 (149769-25-5) ; rac1 GTP-Binding Protein (EC 3.6.5.2) ; rhoA GTP-Binding Protein (EC 3.6.5.2)
    Sprache Englisch
    Erscheinungsdatum 2014-07
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1002702-6
    ISSN 1873-3913 ; 0898-6568
    ISSN (online) 1873-3913
    ISSN 0898-6568
    DOI 10.1016/j.cellsig.2014.03.012
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 2579: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  8. Artikel ; Online: Dual role of ERK5 in the regulation of T cell receptor expression at the T cell surface.

    Rovira-Clavé, Xavier / Angulo-Ibáñez, Maria / Tournier, Cathy / Reina, Manuel / Espel, Enric

    Journal of leukocyte biology

    2016  Band 99, Heft 1, Seite(n) 143–152

    Abstract: Regulation of the levels of the TCR/CD3 complex at the cell surface is critical to proper T cell development and mature T cell activation. We provide evidence that the MAPK ERK5 regulates the surface expression of the TCR/CD3 complex by controlling the ... ...

    Abstract Regulation of the levels of the TCR/CD3 complex at the cell surface is critical to proper T cell development and mature T cell activation. We provide evidence that the MAPK ERK5 regulates the surface expression of the TCR/CD3 complex by controlling the degradation of the CD3ζ chain and the recovery of the complex after anti-CD3ε stimulation. ERK5 knockdown led to TCR/CD3 up-regulation at the cell surface and increased amounts of the CD3ζ chain. Inhibition of the MEK5-dependent phosphorylation status of the kinase domain of ERK5 in human T CD4(+) cells reduced CD3ζ ubiquitination and degradation, limiting TCR/CD3 down-regulation in anti-CD3-stimulated cells. Moreover, TCR/CD3 recovery at the cell surface, after anti-CD3ε treatment, is impaired by ERK5 knockdown or pharmacological inhibition of autophosphorylation in the ERK5 C-terminal region. ERK5 loss in thymocytes augmented cellular CD3ζ and increased cell surface levels of TCR/CD3 on CD4(+)CD8(+) thymocytes. This correlated with enhanced generation of CD4(+)CD8(-)CD25(+) thymocytes. Our findings define ERK5 as a novel kinase that modulates the levels of TCR/CD3 at the cell surface by promoting CD3ζ degradation and TCR/CD3 recovery after TCR stimulation.
    Mesh-Begriff(e) Animals ; Antibodies, Monoclonal/pharmacology ; CD3 Complex/immunology ; CD3 Complex/metabolism ; CD4-Positive T-Lymphocytes/drug effects ; CD4-Positive T-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/metabolism ; Cell Line ; Cell Membrane/metabolism ; Down-Regulation ; Gene Expression Regulation ; Gene Knockdown Techniques ; Humans ; Jurkat Cells ; Mice ; Mice, Knockout ; Mice, Transgenic ; Mitogen-Activated Protein Kinase 7/antagonists & inhibitors ; Mitogen-Activated Protein Kinase 7/genetics ; Mitogen-Activated Protein Kinase 7/metabolism ; Proteolysis ; Receptors, Antigen, T-Cell/genetics ; Receptors, Antigen, T-Cell/metabolism ; T-Lymphocytes/drug effects ; T-Lymphocytes/immunology ; T-Lymphocytes/metabolism ; T-Lymphocytes, Regulatory/immunology ; T-Lymphocytes, Regulatory/metabolism ; Thymocytes/drug effects ; Thymocytes/immunology ; Thymocytes/metabolism ; Ubiquitination
    Chemische Substanzen Antibodies, Monoclonal ; CD3 Complex ; Receptors, Antigen, T-Cell ; Mitogen-Activated Protein Kinase 7 (EC 2.7.11.24)
    Sprache Englisch
    Erscheinungsdatum 2016-01
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605722-6
    ISSN 1938-3673 ; 0741-5400
    ISSN (online) 1938-3673
    ISSN 0741-5400
    DOI 10.1189/jlb.2A0115-034R
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 603: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  9. Artikel ; Online: Spatial epitope barcoding reveals clonal tumor patch behaviors.

    Rovira-Clavé, Xavier / Drainas, Alexandros P / Jiang, Sizun / Bai, Yunhao / Baron, Maya / Zhu, Bokai / Dallas, Alec E / Lee, Myung Chang / Chu, Theresa P / Holzem, Alessandra / Ayyagari, Ramya / Bhattacharya, Debadrita / McCaffrey, Erin F / Greenwald, Noah F / Markovic, Maxim / Coles, Garry L / Angelo, Michael / Bassik, Michael C / Sage, Julien /
    Nolan, Garry P

    Cancer cell

    2022  Band 40, Heft 11, Seite(n) 1423–1439.e11

    Abstract: Intratumoral heterogeneity is a seminal feature of human tumors contributing to tumor progression and response to treatment. Current technologies are still largely unsuitable to accurately track phenotypes and clonal evolution within tumors, especially ... ...

    Abstract Intratumoral heterogeneity is a seminal feature of human tumors contributing to tumor progression and response to treatment. Current technologies are still largely unsuitable to accurately track phenotypes and clonal evolution within tumors, especially in response to genetic manipulations. Here, we developed epitopes for imaging using combinatorial tagging (EpicTags), which we coupled to multiplexed ion beam imaging (EpicMIBI) for in situ tracking of barcodes within tissue microenvironments. Using EpicMIBI, we dissected the spatial component of cell lineages and phenotypes in xenograft models of small cell lung cancer. We observed emergent properties from mixed clones leading to the preferential expansion of clonal patches for both neuroendocrine and non-neuroendocrine cancer cell states in these models. In a tumor model harboring a fraction of PTEN-deficient cancer cells, we observed a non-autonomous increase of clonal patch size in PTEN wild-type cancer cells. EpicMIBI facilitates in situ interrogation of cell-intrinsic and cell-extrinsic processes involved in intratumoral heterogeneity.
    Mesh-Begriff(e) Humans ; Epitopes ; Neoplasms/pathology ; Clonal Evolution ; Clone Cells/pathology ; Cell Lineage ; Tumor Microenvironment
    Chemische Substanzen Epitopes
    Sprache Englisch
    Erscheinungsdatum 2022-10-13
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2078448-X
    ISSN 1878-3686 ; 1535-6108
    ISSN (online) 1878-3686
    ISSN 1535-6108
    DOI 10.1016/j.ccell.2022.09.014
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 5650: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 104: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  10. Artikel ; Online: Absence of ERK5/MAPK7 delays tumorigenesis in Atm-/- mice.

    Granados-Jaén, Alba / Angulo-Ibáñez, Maria / Rovira-Clavé, Xavier / Gamez, Celina Paola Vasquez / Soriano, Francesc X / Reina, Manuel / Espel, Enric

    Oncotarget

    2016  Band 7, Heft 46, Seite(n) 74435–74447

    Abstract: Ataxia-telangiectasia mutated (ATM) is a cell cycle checkpoint kinase that upon activation by DNA damage leads to cell cycle arrest and DNA repair or apoptosis. The absence of Atm or the occurrence of loss-of-function mutations in Atm predisposes to ... ...

    Abstract Ataxia-telangiectasia mutated (ATM) is a cell cycle checkpoint kinase that upon activation by DNA damage leads to cell cycle arrest and DNA repair or apoptosis. The absence of Atm or the occurrence of loss-of-function mutations in Atm predisposes to tumorigenesis. MAPK7 has been implicated in numerous types of cancer with pro-survival and pro-growth roles in tumor cells, but its functional relation with tumor suppressors is not clear. In this study, we show that absence of MAPK7 delays death due to spontaneous tumor development in Atm-/- mice. Compared with Atm-/- thymocytes, Mapk7-/-Atm-/- thymocytes exhibited an improved response to DNA damage (increased phosphorylation of H2AX) and a restored apoptotic response after treatment of mice with ionizing radiation. These findings define an antagonistic function of ATM and MAPK7 in the thymocyte response to DNA damage, and suggest that the lack of MAPK7 inhibits thymic lymphoma growth in Atm-/- mice by partially restoring the DNA damage response in thymocytes.
    Mesh-Begriff(e) Animals ; Ataxia Telangiectasia Mutated Proteins/deficiency ; Ataxia Telangiectasia Mutated Proteins/genetics ; B-Lymphocytes/metabolism ; B-Lymphocytes/pathology ; Cell Cycle/genetics ; Cell Transformation, Neoplastic/genetics ; Cell Transformation, Neoplastic/metabolism ; DNA Damage/genetics ; DNA Damage/radiation effects ; Gene Deletion ; Gene Expression ; Hematopoiesis/genetics ; Histones/metabolism ; Mice ; Mice, Knockout ; Mitogen-Activated Protein Kinase 7/genetics ; Mitogen-Activated Protein Kinase 7/metabolism ; Mutation ; Phosphorylation ; Radiation, Ionizing ; Signal Transduction ; Thymocytes/metabolism ; Thymocytes/pathology
    Chemische Substanzen Histones ; Ataxia Telangiectasia Mutated Proteins (EC 2.7.11.1) ; Mitogen-Activated Protein Kinase 7 (EC 2.7.11.24)
    Sprache Englisch
    Erscheinungsdatum 2016-10-25
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2560162-3
    ISSN 1949-2553 ; 1949-2553
    ISSN (online) 1949-2553
    ISSN 1949-2553
    DOI 10.18632/oncotarget.12908
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Zum Seitenanfang