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  1. Article ; Online: Thiol redox switches regulate the oligomeric state of cyanobacterial Rre1, RpaA and RpaB response regulators.

    Ibrahim, Iskander M / Rowden, Stephen J L / Cramer, William A / Howe, Christopher J / Puthiyaveetil, Sujith

    FEBS letters

    2022  Volume 596, Issue 12, Page(s) 1533–1543

    Abstract: Cyanobacteria employ two-component sensor-response regulator systems to monitor and respond to environmental challenges. The response regulators RpaA, RpaB, Rre1 and RppA are integral to circadian clock function and abiotic stress acclimation in ... ...

    Abstract Cyanobacteria employ two-component sensor-response regulator systems to monitor and respond to environmental challenges. The response regulators RpaA, RpaB, Rre1 and RppA are integral to circadian clock function and abiotic stress acclimation in cyanobacteria. RpaA, RpaB and Rre1 are known to interact with ferredoxin or thioredoxin, raising the possibility of their thiol regulation. Here, we report that Synechocystis sp. PCC 6803 Rre1, RpaA and RpaB exist as higher-order oligomers under oxidising conditions and that reduced thioredoxin A converts them to monomers. We further show that these response regulators contain redox-responsive cysteine residues with an E
    MeSH term(s) Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cyanobacteria/genetics ; Cyanobacteria/metabolism ; Gene Expression Regulation, Bacterial/genetics ; Oxidation-Reduction ; Sulfhydryl Compounds ; Synechocystis/genetics ; Synechocystis/metabolism ; Thioredoxins/genetics ; Thioredoxins/metabolism
    Chemical Substances Bacterial Proteins ; Sulfhydryl Compounds ; Thioredoxins (52500-60-4)
    Language English
    Publishing date 2022-04-11
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 212746-5
    ISSN 1873-3468 ; 0014-5793
    ISSN (online) 1873-3468
    ISSN 0014-5793
    DOI 10.1002/1873-3468.14340
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  2. Article: Thiol redox switches regulate the oligomeric state of cyanobacterial Rre1, RpaA and RpaB response regulators

    Ibrahim, Iskander M. / Rowden, Stephen J. L. / Cramer, William A. / Howe, Christopher J. / Puthiyaveetil, Sujith

    FEBS letters. 2022 June, v. 596, no. 12

    2022  

    Abstract: Cyanobacteria employ two‐component sensor‐response regulator systems to monitor and respond to environmental challenges. The response regulators RpaA, RpaB, Rre1 and RppA are integral to circadian clock function and abiotic stress acclimation in ... ...

    Abstract Cyanobacteria employ two‐component sensor‐response regulator systems to monitor and respond to environmental challenges. The response regulators RpaA, RpaB, Rre1 and RppA are integral to circadian clock function and abiotic stress acclimation in cyanobacteria. RpaA, RpaB and Rre1 are known to interact with ferredoxin or thioredoxin, raising the possibility of their thiol regulation. Here, we report that Synechocystis sp. PCC 6803 Rre1, RpaA and RpaB exist as higher‐order oligomers under oxidising conditions and that reduced thioredoxin A converts them to monomers. We further show that these response regulators contain redox‐responsive cysteine residues with an Eₘ₇ around −300 mV. These findings suggest a direct thiol modulation of the activity of these response regulators, independent of their cognate sensor kinases.
    Keywords abiotic stress ; acclimation ; circadian clocks ; cysteine ; phosphotransferases (kinases) ; thiols ; thioredoxins
    Language English
    Dates of publication 2022-06
    Size p. 1533-1543.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 212746-5
    ISSN 1873-3468 ; 0014-5793
    ISSN (online) 1873-3468
    ISSN 0014-5793
    DOI 10.1002/1873-3468.14340
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  3. Article ; Online: Biophotovoltaics: Design and Study of Bioelectrochemical Systems for Biotechnological Applications and Metabolic Investigation.

    Rowden, Stephen J L / Bombelli, Paolo / Howe, Christopher J

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1770, Page(s) 335–346

    Abstract: Biophotovoltaic methods rely on the fact that photosynthetic microorganisms, like many others, can export small amounts of electric current. For photosynthetic organisms, this current usually increases on illumination. This "exoelectrogenic" property may ...

    Abstract Biophotovoltaic methods rely on the fact that photosynthetic microorganisms, like many others, can export small amounts of electric current. For photosynthetic organisms, this current usually increases on illumination. This "exoelectrogenic" property may be of biotechnological interest, and may also provide useful experimental insights into the physiological status of the cell. We describe how to construct biophotovoltaic devices, and the kinds of measurements that are typically made.
    MeSH term(s) Biotechnology/instrumentation ; Biotechnology/methods ; Cyanobacteria/physiology ; Electrochemistry/instrumentation ; Electrochemistry/methods ; Energy Metabolism ; Photosynthesis
    Language English
    Publishing date 2018-06-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7786-4_20
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  4. Article ; Online: Adaptive laboratory evolution of the fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for improved solvent tolerance.

    Srivastava, Vaibhav / Amanna, Ruth / Rowden, Stephen J L / Sengupta, Shinjinee / Madhu, Swati / Howe, Christopher J / Wangikar, Pramod P

    Journal of bioscience and bioengineering

    2021  Volume 131, Issue 5, Page(s) 491–500

    Abstract: Cyanobacteria hold promise as cell factories for the photoautotrophic conversion of carbon dioxide to useful chemicals. For the eventual commercial viability of such processes, cyanobacteria need to be engineered for (i) efficient channeling of carbon ... ...

    Abstract Cyanobacteria hold promise as cell factories for the photoautotrophic conversion of carbon dioxide to useful chemicals. For the eventual commercial viability of such processes, cyanobacteria need to be engineered for (i) efficient channeling of carbon flux toward the product of interest and (ii) improved product tolerance, the latter being the focus of this study. We chose the recently reported, fast-growing, high light and CO
    MeSH term(s) Alcohols/pharmacology ; Biofuels ; Carbon Dioxide/metabolism ; Directed Molecular Evolution ; Photosynthesis/drug effects ; Solvents/pharmacology ; Synechococcus/drug effects ; Synechococcus/genetics ; Synechococcus/metabolism
    Chemical Substances Alcohols ; Biofuels ; Solvents ; Carbon Dioxide (142M471B3J)
    Language English
    Publishing date 2021-02-18
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 1465387-4
    ISSN 1347-4421 ; 1389-1723
    ISSN (online) 1347-4421
    ISSN 1389-1723
    DOI 10.1016/j.jbiosc.2020.11.012
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    In stock of ZB MED Bonn / Germany

    Z 4728: Show issues

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  5. Article ; Online: Integrated Genomic and Transcriptomic Analysis of the Peridinin Dinoflagellate Amphidinium carterae Plastid.

    Dorrell, Richard G / Nisbet, R Ellen R / Barbrook, Adrian C / Rowden, Stephen J L / Howe, Christopher J

    Protist

    2019  Volume 170, Issue 4, Page(s) 358–373

    Abstract: The plastid genomes of peridinin-containing dinoflagellates are highly unusual, possessing very few genes, which are located on small chromosomal elements termed "minicircles". These minicircles may contain genes, or no recognisable coding information. ... ...

    Abstract The plastid genomes of peridinin-containing dinoflagellates are highly unusual, possessing very few genes, which are located on small chromosomal elements termed "minicircles". These minicircles may contain genes, or no recognisable coding information. Transcripts produced from minicircles may undergo unusual processing events, such as the addition of a 3' poly(U) tail. To date, little is known about the genetic or transcriptional diversity of non-coding sequences in peridinin dinoflagellate plastids. These sequences include empty minicircles, and regions of non-coding DNA in coding minicircles. Here, we present an integrated plastid genome and transcriptome for the model peridinin dinoflagellate Amphidinium carterae, identifying a previously undescribed minicircle. We also profile transcripts covering non-coding regions of the psbA and petB/atpA minicircles. We present evidence that antisense transcripts are produced within the A. carterae plastid, but show that these transcripts undergo different end cleavage events from sense transcripts, and do not receive 3' poly(U) tails. The difference in processing events between sense and antisense transcripts may enable the removal of non-coding transcripts from peridinin dinoflagellate plastid transcript pools.
    MeSH term(s) Dinoflagellida/classification ; Dinoflagellida/genetics ; Gene Expression Profiling ; Genome, Protozoan/genetics ; Genomics ; Plastids/genetics ; Transcriptome/genetics
    Language English
    Publishing date 2019-07-05
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2036014-9
    ISSN 1618-0941 ; 1434-4610
    ISSN (online) 1618-0941
    ISSN 1434-4610
    DOI 10.1016/j.protis.2019.06.001
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  6. Article: Integrated Genomic and Transcriptomic Analysis of the Peridinin Dinoflagellate Amphidinium carterae Plastid

    Dorrell, Richard G / Barbrook, Adrian C / Howe, Christopher J / Nisbet, R. Ellen R / Rowden, Stephen J.L

    Protist. 2019 Aug., v. 170, no. 4

    2019  

    Abstract: The plastid genomes of peridinin-containing dinoflagellates are highly unusual, possessing very few genes, which are located on small chromosomal elements termed “minicircles”. These minicircles may contain genes, or no recognisable coding information. ... ...

    Abstract The plastid genomes of peridinin-containing dinoflagellates are highly unusual, possessing very few genes, which are located on small chromosomal elements termed “minicircles”. These minicircles may contain genes, or no recognisable coding information. Transcripts produced from minicircles may undergo unusual processing events, such as the addition of a 3' poly(U) tail. To date, little is known about the genetic or transcriptional diversity of non-coding sequences in peridinin dinoflagellate plastids. These sequences include empty minicircles, and regions of non-coding DNA in coding minicircles. Here, we present an integrated plastid genome and transcriptome for the model peridinin dinoflagellate Amphidinium carterae, identifying a previously undescribed minicircle. We also profile transcripts covering non-coding regions of the psbA and petB/atpA minicircles. We present evidence that antisense transcripts are produced within the A. carterae plastid, but show that these transcripts undergo different end cleavage events from sense transcripts, and do not receive 3' poly(U) tails. The difference in processing events between sense and antisense transcripts may enable the removal of non-coding transcripts from peridinin dinoflagellate plastid transcript pools.
    Keywords Amphidinium ; genes ; genomics ; intergenic DNA ; models ; non-coding RNA ; plastid genome ; plastids ; tail ; transcription (genetics) ; transcriptome ; transcriptomics
    Language English
    Dates of publication 2019-08
    Size p. 358-373.
    Publishing place Elsevier GmbH
    Document type Article
    ZDB-ID 2036014-9
    ISSN 1618-0941 ; 1434-4610
    ISSN (online) 1618-0941
    ISSN 1434-4610
    DOI 10.1016/j.protis.2019.06.001
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  7. Article: The Development of Biophotovoltaic Systems for Power Generation and Biological Analysis.

    Wey, Laura T / Bombelli, Paolo / Chen, Xiaolong / Lawrence, Joshua M / Rabideau, Clayton M / Rowden, Stephen J L / Zhang, Jenny Z / Howe, Christopher J

    ChemElectroChem

    2019  Volume 6, Issue 21, Page(s) 5375–5386

    Abstract: Biophotovoltaic systems (BPVs) resemble microbial fuel cells, but utilise oxygenic photosynthetic microorganisms associated with an anode to generate an extracellular electrical current, which is stimulated by illumination. Study and exploitation of BPVs ...

    Abstract Biophotovoltaic systems (BPVs) resemble microbial fuel cells, but utilise oxygenic photosynthetic microorganisms associated with an anode to generate an extracellular electrical current, which is stimulated by illumination. Study and exploitation of BPVs have come a long way over the last few decades, having benefited from several generations of electrode development and improvements in wiring schemes. Power densities of up to 0.5 W m
    Language English
    Publishing date 2019-09-18
    Publishing country Germany
    Document type Journal Article ; Review
    ISSN 2196-0216
    ISSN 2196-0216
    DOI 10.1002/celc.201900997
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  8. Article ; Online: Biological photovoltaics: intra- and extra-cellular electron transport by cyanobacteria.

    Bradley, Robert W / Bombelli, Paolo / Rowden, Stephen J L / Howe, Christopher J

    Biochemical Society transactions

    2012  Volume 40, Issue 6, Page(s) 1302–1307

    Abstract: A large variety of new energy-generating technologies are being developed in an effort to reduce global dependence on fossil fuels, and to reduce the carbon footprint of energy generation. The term 'biological photovoltaic system' encompasses a broad ... ...

    Abstract A large variety of new energy-generating technologies are being developed in an effort to reduce global dependence on fossil fuels, and to reduce the carbon footprint of energy generation. The term 'biological photovoltaic system' encompasses a broad range of technologies which all employ biological material that can harness light energy to split water, and then transfer the resulting electrons to an anode for power generation or electrosynthesis. The use of whole cyanobacterial cells is a good compromise between the requirements of the biological material to be simply organized and transfer electrons efficiently to the anode, and also to be robust and able to self-assemble and self-repair. The principle that photosynthetic bacteria can generate and transfer electrons directly or indirectly to an anode has been demonstrated by a number of groups, although the power output obtained from these devices is too low for biological photovoltaic devices to be useful outside the laboratory. Understanding how photosynthetically generated electrons are transferred through and out of the organism is key to improving power output, and investigations on this aspect of the technology are the main focus of the present review.
    MeSH term(s) Bioelectric Energy Sources ; Cyanobacteria/metabolism ; Cyanobacteria/physiology ; Electron Transport ; Oxidation-Reduction ; Photosynthesis
    Language English
    Publishing date 2012-12-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 184237-7
    ISSN 1470-8752 ; 0300-5127
    ISSN (online) 1470-8752
    ISSN 0300-5127
    DOI 10.1042/BST20120118
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 944: Show issues Location:
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  9. Article ; Online: Surface morphology and surface energy of anode materials influence power outputs in a multi-channel mediatorless bio-photovoltaic (BPV) system.

    Bombelli, Paolo / Zarrouati, Marie / Thorne, Rebecca J / Schneider, Kenneth / Rowden, Stephen J L / Ali, Akin / Yunus, Kamran / Cameron, Petra J / Fisher, Adrian C / Ian Wilson, D / Howe, Christopher J / McCormick, Alistair J

    Physical chemistry chemical physics : PCCP

    2012  Volume 14, Issue 35, Page(s) 12221–12229

    Abstract: Bio-photovoltaic cells (BPVs) are a new photo-bio-electrochemical technology for harnessing solar energy using the photosynthetic activity of autotrophic organisms. Currently power outputs from BPVs are generally low and suffer from low efficiencies. ... ...

    Abstract Bio-photovoltaic cells (BPVs) are a new photo-bio-electrochemical technology for harnessing solar energy using the photosynthetic activity of autotrophic organisms. Currently power outputs from BPVs are generally low and suffer from low efficiencies. However, a better understanding of the electrochemical interactions between the microbes and conductive materials will be likely to lead to increased power yields. In the current study, the fresh-water, filamentous cyanobacterium Pseudanabaena limnetica (also known as Oscillatoria limnetica) was investigated for exoelectrogenic activity. Biofilms of P. limnetica showed a significant photo response during light-dark cycling in BPVs under mediatorless conditions. A multi-channel BPV device was developed to compare quantitatively the performance of photosynthetic biofilms of this species using a variety of different anodic conductive materials: indium tin oxide-coated polyethylene terephthalate (ITO), stainless steel (SS), glass coated with a conductive polymer (PANI), and carbon paper (CP). Although biofilm growth rates were generally comparable on all materials tested, the amplitude of the photo response and achievable maximum power outputs were significantly different. ITO and SS demonstrated the largest photo responses, whereas CP showed the lowest power outputs under both light and dark conditions. Furthermore, differences in the ratios of light : dark power outputs indicated that the electrochemical interactions between photosynthetic microbes and the anode may differ under light and dark conditions depending on the anodic material used. Comparisons between BPV performances and material characteristics revealed that surface roughness and surface energy, particularly the ratio of non-polar to polar interactions (the CQ ratio), may be more important than available surface area in determining biocompatibility and maximum power outputs in microbial electrochemical systems. Notably, CP was readily outperformed by all other conductive materials tested, indicating that carbon may not be an optimal substrate for microbial fuel cell operation.
    MeSH term(s) Bioelectric Energy Sources/microbiology ; Biofilms ; Cyanobacteria/physiology ; Electrodes ; Equipment Design ; Light ; Photosynthesis ; Solar Energy ; Surface Properties
    Language English
    Publishing date 2012-09-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1476244-4
    ISSN 1463-9084 ; 1463-9076
    ISSN (online) 1463-9084
    ISSN 1463-9076
    DOI 10.1039/c2cp42526b
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