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Article ; Online: Specificity and genetic polymorphism in the Vfm quorum sensing system of plant pathogenic bacteria of the genus Dickeya.

Hugouvieux-Cotte-Pattat, Nicole / Royer, Monique / Gueguen, Erwan / Le Guen, Paul / Süssmuth, Roderich D / Reverchon, Sylvie / Cociancich, Stéphane

Environmental microbiology

2022 Volume 24, Issue 3, Page(s) 1467–1483

Abstract: The Vfm quorum sensing (QS) system is preponderant for the virulence of different species of the bacterial genus Dickeya. The vfm gene cluster encodes 26 genes involved in the production, sensing or transduction of the QS signal. To date, the Vfm QS ... ...

Abstract	The Vfm quorum sensing (QS) system is preponderant for the virulence of different species of the bacterial genus Dickeya. The vfm gene cluster encodes 26 genes involved in the production, sensing or transduction of the QS signal. To date, the Vfm QS signal has escaped detection by analytical chemistry methods. However, we report here a strain-specific polymorphism in the biosynthesis genes vfmO and vfmP, which is predicted to be related to the production of different analogues of the QS signal. Consequently, the Vfm communication could be impossible between strains possessing different variants of the genes vfmO/P. We constructed three Vfm QS biosensor strains possessing different vfmO/P variants and compared these biosensors for their responses to samples prepared from 34 Dickeya strains possessing different vfmO/P variants. A pattern of specificity was demonstrated, providing evidence that the polymorphism in the genes vfmO/P determines the biosynthesis of different analogues of the QS signal. Unexpectedly, this vfmO/P-dependent pattern of specificity is linked to a polymorphism in the ABC transporter gene vfmG, suggesting an adaptation of the putative permease VfmG to specifically bind different analogues of the QS signal. Accordingly, we discuss the possible involvement of VfmG as co-sensor of the Vfm two-component regulatory system.
MeSH term(s)	Bacteria/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Dickeya ; Gene Expression Regulation, Bacterial ; Polymorphism, Genetic ; Quorum Sensing/genetics
Chemical Substances	Bacterial Proteins
Language	English
Publishing date	2022-01-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2020213-1
ISSN	1462-2920 ; 1462-2912
ISSN (online)	1462-2920
ISSN	1462-2912
DOI	10.1111/1462-2920.15889
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Specificity and genetic polymorphism in the Vfm quorum sensing system of plant pathogenic bacteria of the genus Dickeya

Hugouvieux‐Cotte‐Pattat, Nicole / Royer, Monique / Gueguen, Erwan / Le Guen, Paul / Süssmuth, Roderich D. / Reverchon, Sylvie / Cociancich, Stéphane

Environmental microbiology. 2022 Mar., v. 24, no. 3

2022

Abstract	The Vfm quorum sensing (QS) system is preponderant for the virulence of different species of the bacterial genus Dickeya. The vfm gene cluster encodes 26 genes involved in the production, sensing or transduction of the QS signal. To date, the Vfm QS signal has escaped detection by analytical chemistry methods. However, we report here a strain‐specific polymorphism in the biosynthesis genes vfmO and vfmP, which is predicted to be related to the production of different analogues of the QS signal. Consequently, the Vfm communication could be impossible between strains possessing different variants of the genes vfmO/P. We constructed three Vfm QS biosensor strains possessing different vfmO/P variants and compared these biosensors for their responses to samples prepared from 34 Dickeya strains possessing different vfmO/P variants. A pattern of specificity was demonstrated, providing evidence that the polymorphism in the genes vfmO/P determines the biosynthesis of different analogues of the QS signal. Unexpectedly, this vfmO/P‐dependent pattern of specificity is linked to a polymorphism in the ABC transporter gene vfmG, suggesting an adaptation of the putative permease VfmG to specifically bind different analogues of the QS signal. Accordingly, we discuss the possible involvement of VfmG as co‐sensor of the Vfm two‐component regulatory system.
Keywords	ABC transporters ; Dickeya ; analytical chemistry ; biosensors ; biosynthesis ; genetic polymorphism ; multigene family ; virulence
Language	English
Dates of publication	2022-03
Size	p. 1467-1483.
Publishing place	John Wiley & Sons, Inc.
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2020213-1
ISSN	1462-2920 ; 1462-2912
ISSN (online)	1462-2920
ISSN	1462-2912
DOI	10.1111/1462-2920.15889
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Article ; Online: A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria.

Brual, Typhaine / Effantin, Géraldine / Baltenneck, Julie / Attaiech, Laetitia / Grosbois, Cloé / Royer, Monique / Cigna, Jérémy / Faure, Denis / Hugouvieux-Cotte-Pattat, Nicole / Gueguen, Erwan

PLoS genetics

2023 Volume 19, Issue 4, Page(s) e1010725

Abstract: The necrotrophic plant pathogenic bacterium Dickeya solani emerged in the potato agrosystem in Europe. All isolated strains of D. solani contain several large polyketide synthase/non-ribosomal peptide synthetase (PKS/NRPS) gene clusters. Analogy with ... ...

Abstract	The necrotrophic plant pathogenic bacterium Dickeya solani emerged in the potato agrosystem in Europe. All isolated strains of D. solani contain several large polyketide synthase/non-ribosomal peptide synthetase (PKS/NRPS) gene clusters. Analogy with genes described in other bacteria suggests that the clusters ooc and zms are involved in the production of secondary metabolites of the oocydin and zeamine families, respectively. A third cluster named sol was recently shown to produce an antifungal molecule. In this study, we constructed mutants impaired in each of the three secondary metabolite clusters sol, ooc, and zms to compare first the phenotype of the D. solani wild-type strain D s0432-1 with its associated mutants. We demonstrated the antimicrobial functions of these three PKS/NRPS clusters against bacteria, yeasts or fungi. The cluster sol, conserved in several other Dickeya species, produces a secondary metabolite inhibiting yeasts. Phenotyping and comparative genomics of different D. solani wild-type isolates revealed that the small regulatory RNA ArcZ plays a major role in the control of the clusters sol and zms. A single-point mutation, conserved in some Dickeya wild-type strains, including the D. solani type strain IPO 2222, impairs the ArcZ function by affecting its processing into an active form.
MeSH term(s)	Multigene Family/genetics ; Point Mutation ; Genomics ; RNA, Bacterial/genetics ; RNA, Bacterial/metabolism ; Polyketide Synthases/genetics ; Antimicrobial Peptides/genetics ; Antimicrobial Peptides/pharmacology ; Bacteria/drug effects ; Ascomycota/drug effects ; Dickeya/genetics ; Dickeya/metabolism ; Gene Expression Regulation, Bacterial/genetics
Chemical Substances	RNA, Bacterial ; Polyketide Synthases (79956-01-7) ; non-ribosomal peptide synthase (EC 6.3.2.-) ; Antimicrobial Peptides
Language	English
Publishing date	2023-04-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	2186725-2
ISSN	1553-7404 ; 1553-7390
ISSN (online)	1553-7404
ISSN	1553-7390
DOI	10.1371/journal.pgen.1010725
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Article: Geographical Distribution of Four Sugarcane yellow leaf virus Genotypes.

Ahmad, Youssef Abu / Royer, Monique / Daugrois, Jean-Henrich / Costet, Laurent / Lett, Jean-Michel / Victoria, Jorge I / Girard, Jean-Claude / Rott, Philippe

Plant disease

2019 Volume 90, Issue 9, Page(s) 1156–1160

Abstract: Specific primer pairs were designed to distinguish four genotypes (BRA for Brazil, CUB for Cuba, PER for Peru, and REU for Réunion Island) of Sugarcane yellow leaf virus (SCYLV) by reverse transcription-polymerase chain reaction (RT-PCR). A unique genome ...

Abstract	Specific primer pairs were designed to distinguish four genotypes (BRA for Brazil, CUB for Cuba, PER for Peru, and REU for Réunion Island) of Sugarcane yellow leaf virus (SCYLV) by reverse transcription-polymerase chain reaction (RT-PCR). A unique genome fragment was amplified from each genotype, with the exception of genotypes BRA and PER that are phylogenetically relatively close and were designated genotype BRA-PER. These RT-PCR primers were then used to identify the SCYLV genotype(s) present in 18 different sugarcane growing locations in the world, and 245 leaf samples infected by the virus were analyzed. Most samples were infected by only one of the three genotypes, but mixed infections occurred. Genotype BRA-PER was found in all sugarcane growing locations, whereas genotypes CUB and REU were each found in four geographical locations only. Genotypes BRA-PER, CUB, and REU were all three detected in locally bred sugarcane cultivars in Guadeloupe, indicating local transmission of these genotypes. In contrast, only genotypes BRA-PER and CUB were found in locally bred cultivars in Brazil, whereas genotype REU was detected in this country in cultivar R570 imported from Réunion. Similarly, genotypes BRA-PER and REU are both present in Réunion, but genotype BRA-PER has not, as of yet, spread on this island. Presence of several SCYLV genotypes in Brazil, Colombia, Guadeloupe, Mauritius, and Réunion suggests different virus introductions and/or different evolution histories of the virus after its introduction into a new environment.
Language	English
Publishing date	2019-02-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PD-90-1156
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Article: Draft Genome Sequence of Xanthomonas sacchari Strain LMG 476

Pieretti, Isabelle / Bolot, Stéphanie / Carrééère, Séébastien / Barbe, Valééèérie / Cociancich, Stééèééphane / Rott, Philippe / Royer, Monique

Genome Announcements. 2015 Apr. 30, v. 3, no. 2

2015

Abstract: We report the high-quality draft genome sequence of Xanthomonas sacchari strain LMG 476, isolated from sugarcane. The genome comparison of this strain with a previously sequenced X. sacchari strain isolated from a distinct environmental source should ... ...

Abstract	We report the high-quality draft genome sequence of Xanthomonas sacchari strain LMG 476, isolated from sugarcane. The genome comparison of this strain with a previously sequenced X. sacchari strain isolated from a distinct environmental source should provide further insights into the adaptation of this species to different habitats and its evolution.
Keywords	Xanthomonas sacchari ; evolution ; genome ; habitats ; nucleotide sequences ; sugarcane
Language	English
Dates of publication	2015-0430
Size	p. e00146-15.
Publishing place	American Society for Microbiology
Document type	Article
ZDB-ID	2704277-7
ISSN	2169-8287
ISSN	2169-8287
DOI	10.1128/genomeA.00146-15
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Article: Draft Genome Sequence of Xanthomonas sacchari Strain LMG 476.

Pieretti, Isabelle / Bolot, Stéphanie / Carrère, Sébastien / Barbe, Valérie / Cociancich, Stéphane / Rott, Philippe / Royer, Monique

Genome announcements

2015 Volume 3, Issue 2

Abstract	We report the high-quality draft genome sequence of Xanthomonas sacchari strain LMG 476, isolated from sugarcane. The genome comparison of this strain with a previously sequenced X. sacchari strain isolated from a distinct environmental source should provide further insights into the adaptation of this species to different habitats and its evolution.
Language	English
Publishing date	2015-03-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2704277-7
ISSN	2169-8287
ISSN	2169-8287
DOI	10.1128/genomeA.00146-15
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Article: What makes Xanthomonas albilineans unique amongst xanthomonads?

Pieretti, Isabelle / Pesic, Alexander / Petras, Daniel / Royer, Monique / Süssmuth, Roderich D / Cociancich, Stéphane

Frontiers in plant science

2015 Volume 6, Page(s) 289

Abstract: Xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. Compared to other species of Xanthomonas, X. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy. Its genome, which has experienced significant erosion, ... ...

Abstract	Xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. Compared to other species of Xanthomonas, X. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy. Its genome, which has experienced significant erosion, has unique genomic features. It lacks two loci required for pathogenicity in other plant pathogenic species of Xanthomonas: the xanthan gum biosynthesis and the Hrp-T3SS (hypersensitive response and pathogenicity-type three secretion system) gene clusters. Instead, X. albilineans harbors in its genome an SPI-1 (Salmonella pathogenicity island-1) T3SS gene cluster usually found in animal pathogens. X. albilineans produces a potent DNA gyrase inhibitor called albicidin, which blocks chloroplast differentiation, resulting in the characteristic white foliar stripe symptoms. The antibacterial activity of albicidin also confers on X. albilineans a competitive advantage against rival bacteria during sugarcane colonization. Recent chemical studies have uncovered the unique structure of albicidin and allowed us to partially elucidate its fascinating biosynthesis apparatus, which involves an enigmatic hybrid PKS/NRPS (polyketide synthase/non-ribosomal peptide synthetase) machinery.
Language	English
Publishing date	2015-04-24
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2613694-6
ISSN	1664-462X
ISSN	1664-462X
DOI	10.3389/fpls.2015.00289
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Article: Full Genome Sequence Analysis of Two Isolates Reveals a Novel Xanthomonas Species Close to the Sugarcane Pathogen Xanthomonas albilineans.

Pieretti, Isabelle / Cociancich, Stéphane / Bolot, Stéphanie / Carrère, Sébastien / Morisset, Alexandre / Rott, Philippe / Royer, Monique

Genes

2015 Volume 6, Issue 3, Page(s) 714–733

Abstract: Xanthomonas albilineans is the bacterium responsible for leaf scald, a lethal disease of sugarcane. Within the Xanthomonas genus, X. albilineans exhibits distinctive genomic characteristics including the presence of significant genome erosion, a non- ... ...

Abstract	Xanthomonas albilineans is the bacterium responsible for leaf scald, a lethal disease of sugarcane. Within the Xanthomonas genus, X. albilineans exhibits distinctive genomic characteristics including the presence of significant genome erosion, a non-ribosomal peptide synthesis (NRPS) locus involved in albicidin biosynthesis, and a type 3 secretion system (T3SS) of the Salmonella pathogenicity island-1 (SPI-1) family. We sequenced two X. albilineans-like strains isolated from unusual environments, i.e., from dew droplets on sugarcane leaves and from the wild grass Paspalum dilatatum, and compared these genomes sequences with those of two strains of X. albilineans and three of Xanthomonas sacchari. Average nucleotide identity (ANI) and multi-locus sequence analysis (MLSA) showed that both X. albilineans-like strains belong to a new species close to X. albilineans that we have named "Xanthomonas pseudalbilineans". X. albilineans and "X. pseudalbilineans" share many genomic features including (i) the lack of genes encoding a hypersensitive response and pathogenicity type 3 secretion system (Hrp-T3SS), and (ii) genome erosion that probably occurred in a common progenitor of both species. Our comparative analyses also revealed specific genomic features that may help X. albilineans interact with sugarcane, e.g., a PglA endoglucanase, three TonB-dependent transporters and a glycogen metabolism gene cluster. Other specific genomic features found in the "X. pseudalbilineans" genome may contribute to its fitness and specific ecological niche.
Language	English
Publishing date	2015-07-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527218-4
ISSN	2073-4425
ISSN	2073-4425
DOI	10.3390/genes6030714
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Article ; Online: Total synthesis of albicidin: a lead structure from Xanthomonas albilineans for potent antibacterial gyrase inhibitors.

Kretz, Julian / Kerwat, Dennis / Schubert, Vivien / Grätz, Stefan / Pesic, Alexander / Semsary, Siamak / Cociancich, Stéphane / Royer, Monique / Süssmuth, Roderich D

Angewandte Chemie (International ed. in English)

2015 Volume 54, Issue 6, Page(s) 1969–1973

Abstract: The peptide antibiotic albicidin, which is synthesized by the plant pathogenic bacterium Xanthomonas albilineans, displays remarkable antibacterial activity against various Gram-positive and Gram-negative microorganisms. The low amounts of albicidin ... ...

Abstract	The peptide antibiotic albicidin, which is synthesized by the plant pathogenic bacterium Xanthomonas albilineans, displays remarkable antibacterial activity against various Gram-positive and Gram-negative microorganisms. The low amounts of albicidin obtainable from the producing organism or through heterologous expression are limiting factors in providing sufficient material for bioactivity profiling and structure-activity studies. Therefore, we developed a convergent total synthesis route toward albicidin. The unexpectedly difficult formation of amide bonds between the aromatic amino acids was achieved through a triphosgene-mediated coupling strategy. The herein presented synthesis of albicidin confirms the previously determined chemical structure and underlines the extraordinary antibacterial activity of this compound. The synthetic protocol will provide multigram amounts of albicidin for further profiling of its drug properties.
MeSH term(s)	Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; DNA Gyrase/drug effects ; Enzyme Inhibitors/chemistry ; Enzyme Inhibitors/pharmacology ; Molecular Structure ; Organic Chemicals/chemistry ; Organic Chemicals/pharmacology ; Xanthomonas/chemistry
Chemical Substances	Anti-Bacterial Agents ; Enzyme Inhibitors ; Organic Chemicals ; albicidin (96955-97-4) ; DNA Gyrase (EC 5.99.1.3)
Language	English
Publishing date	2015-02-02
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2011836-3
ISSN	1521-3773 ; 1433-7851
ISSN (online)	1521-3773
ISSN	1433-7851
DOI	10.1002/anie.201409584
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Article ; Online: The Albicidin Resistance Factor AlbD Is a Serine Endopeptidase That Hydrolyzes Unusual Oligoaromatic-Type Peptides.

Vieweg, Laura / Kretz, Julian / Pesic, Alexander / Kerwat, Dennis / Grätz, Stefan / Royer, Monique / Cociancich, Stéphane / Mainz, Andi / Süssmuth, Roderich D

Journal of the American Chemical Society

2015 Volume 137, Issue 24, Page(s) 7608–7611

Abstract: The para-aminobenzoic acid-containing peptide albicidin is a pathogenicity factor synthesized by Xanthomonas albilineans in infections of sugar cane. Albicidin is a nanomolar inhibitor of the bacterial DNA gyrase with a strong activity against various ... ...

Abstract	The para-aminobenzoic acid-containing peptide albicidin is a pathogenicity factor synthesized by Xanthomonas albilineans in infections of sugar cane. Albicidin is a nanomolar inhibitor of the bacterial DNA gyrase with a strong activity against various Gram-negative bacteria. The bacterium Pantoea dispersa expresses the hydrolase AlbD, conferring natural resistance against albicidin. We show that AlbD is a novel type of endopeptidase that catalyzes the cleavage of albicidin at a peptide backbone amide bond, thus abolishing its antimicrobial activity. Additionally, we determined the minimal cleavage motif of AlbD with substrates derived by chemical synthesis. Our results clearly identify AlbD as a unique endopeptidase that is the first member of a new subfamily of peptidases. Our findings provide the molecular basis for a natural detoxification mechanism, potentially rendering a new tool in biological chemistry approaches.
MeSH term(s)	Anti-Bacterial Agents/metabolism ; Anti-Bacterial Agents/pharmacology ; Drug Resistance, Bacterial ; Enterobacteriaceae Infections/drug therapy ; Enterobacteriaceae Infections/microbiology ; Humans ; Hydrolysis ; Organic Chemicals/metabolism ; Organic Chemicals/pharmacology ; Pantoea/drug effects ; Pantoea/enzymology ; Serine Endopeptidases/metabolism ; Xanthomonas/metabolism
Chemical Substances	Anti-Bacterial Agents ; Organic Chemicals ; albicidin (96955-97-4) ; Serine Endopeptidases (EC 3.4.21.-)
Language	English
Publishing date	2015-06-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.5b04099
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