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  1. Article: Conditions for sliding of nucleosomes along DNA: SV 40 minichromosomes.

    Glotov, B O / Rudin, A V / Severin, E S

    Biochimica et biophysica acta

    1982  Volume 696, Issue 3, Page(s) 275–284

    Abstract: Sliding' of nucleosomes along DNA under nearly physiological conditions was studied using treatment of SV 40 minichromosomes with the single-cut restriction endonucleases EcoRI and BamHI. Each enzyme can convert no more than 20-25% of the circular DNA ... ...

    Abstract 'Sliding' of nucleosomes along DNA under nearly physiological conditions was studied using treatment of SV 40 minichromosomes with the single-cut restriction endonucleases EcoRI and BamHI. Each enzyme can convert no more than 20-25% of the circular DNA molecules of minichromosomes into the linear form irrespective of the presence of histone H1. This suggests absence of the nucleosomes lateral migration (sliding) along DNa at least in the vicinity of the restriction endonucleases cleavage sites during several hours of incubation. The sites available for EcoRI and BamHI in minichromosomes seem to be located predominantly in the spacer DNA regions of nucleosomes. Introduction of only one double-strand (but not single-strand) break into the DNA of minichromosomes stripped of histone H1 is sufficient to induce redistribution of the nucleosome core particles due to their sliding along DNA. Thus, sliding of the nucleosome core particles can be induced under physiological conditions by rather low energy expenditures.
    MeSH term(s) Animals ; Cell Line ; Cercopithecus aethiops ; Chromosomes/ultrastructure ; DNA Restriction Enzymes ; DNA, Circular/metabolism ; DNA, Viral/metabolism ; Kidney ; Microscopy, Electron ; Nucleosomes/metabolism ; Nucleosomes/ultrastructure ; Simian virus 40/genetics
    Chemical Substances DNA, Circular ; DNA, Viral ; Nucleosomes ; DNA Restriction Enzymes (EC 3.1.21.-)
    Language English
    Publishing date 1982-03-29
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 60-7
    ISSN 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/0167-4781(82)90058-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Immunomorfologicheskaia kharakteristika raspredeleniia kollagena I, III, IV, V tipov v normal'noĭ intime i pri ateroskleroze krupnykh arteriĭ i aorty cheloveka.

    Shekhonin, B V / Domogatskiĭ, S P / Rudin, A V / Rukosuev, V S

    Arkhiv patologii

    1984  Volume 46, Issue 3, Page(s) 18–24

    Abstract: The distribution of collagen of the above types in normal intima, sites of its physiological thickening, lipid streaks, atherosclerotic plaques is studied by the immunoluminescent method. All collagen types at various proportions are found to be located ... ...

    Title translation Immunomorphologic characteristics of the distribution of collagen types I, III, IV and V in normal intima and in atherosclerosis of the major arteries and aorta in man.
    Abstract The distribution of collagen of the above types in normal intima, sites of its physiological thickening, lipid streaks, atherosclerotic plaques is studied by the immunoluminescent method. All collagen types at various proportions are found to be located in the subendothelial areas of intima. Besides that the IV and V collagen types are detected in the zone of endothelial basal membrane. The essential property of atherosclerotic plaques is the prevalence of rough fibrillar structures from interstitial collagen which do not occur normally.
    MeSH term(s) Adult ; Age Factors ; Aged ; Arteries/metabolism ; Arteriosclerosis/metabolism ; Basement Membrane/metabolism ; Collagen/metabolism ; Endothelium/metabolism ; Fluorescent Antibody Technique ; Humans ; Middle Aged
    Chemical Substances Collagen (9007-34-5)
    Language Russian
    Publishing date 1984
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article
    ZDB-ID 127285-8
    ISSN 0004-1955
    ISSN 0004-1955
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  3. Article: Opredelenie antitrombotsitarnykh antitel v syvorotke krovi bol'nykh s idiopaticheskoĭ trombotsitopenicheskoĭ purpuroĭ immunofermentnym metodom.

    Koval', A A / Mazurov, A V / Vinogradov, D V / Rudin, A V / Repin, V S

    Biulleten' eksperimental'noi biologii i meditsiny

    1989  Volume 107, Issue 3, Page(s) 327–329

    Abstract: Enzyme-linked immunosorbent assay (ELISA) was developed for determination of serum antiplatelet antibodies. Platelets obtained from healthy donors of blood group 0(1) were washed off plasma and sedimented on the bottom of microtest wells. After washing ... ...

    Title translation Determination of antithrombocyte antibodies in the blood serum of patients with idiopathic thrombocytopenic purpura by an immunoenzyme method.
    Abstract Enzyme-linked immunosorbent assay (ELISA) was developed for determination of serum antiplatelet antibodies. Platelets obtained from healthy donors of blood group 0(1) were washed off plasma and sedimented on the bottom of microtest wells. After washing off unattached platelets and blocking of plastic with albumin platelets were incubated with sera under investigation and binding of serum antibodies was detected using antihuman immunoglobulin antibodies conjugated with peroxidase. Ten patients with idiopathic thrombocytopenic purpura (ITP). 1 patient with systemic lupus erythematosus. 1 patient with red blood cell aplasia and 9 healthy donors (negative control) were studied by ELISA. Serum antibodies which effectively bound to platelets were detected in 5 patients with ITP, in patient with lupus erythematosus and in patient with red blood cell aplasia.
    MeSH term(s) ABO Blood-Group System/immunology ; Antibodies/analysis ; Blood Platelets/immunology ; Blood Transfusion ; Enzyme-Linked Immunosorbent Assay ; Humans ; Purpura, Thrombocytopenic/immunology ; Purpura, Thrombocytopenic/therapy ; Red-Cell Aplasia, Pure/immunology ; Red-Cell Aplasia, Pure/therapy
    Chemical Substances ABO Blood-Group System ; Antibodies
    Language Russian
    Publishing date 1989-03
    Publishing country Russia (Federation)
    Document type Comparative Study ; English Abstract ; Journal Article
    ZDB-ID 127365-6
    ISSN 0365-9615 ; 0006-4041
    ISSN 0365-9615 ; 0006-4041
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  4. Article: Raspredelenie kollagena I, III, IV, V tipov v stenke arteriĭ cheloveka.

    Shekhonin, B V / Kondalenko, V F / Domogatskiĭ, S P / Rudin, A V / Rukosuev, V S

    Kardiologiia

    1984  Volume 24, Issue 4, Page(s) 95–99

    Abstract: Using immunofluorescence the localization of I, II, IV, V type collagen in different layers of the artery wall was established. The adventitia was shown to contain only I and III type collagen. All collagen types studied were identified in the media. The ...

    Title translation Distribution of collagen types I, III, IV and V in the walls of human arteries.
    Abstract Using immunofluorescence the localization of I, II, IV, V type collagen in different layers of the artery wall was established. The adventitia was shown to contain only I and III type collagen. All collagen types studied were identified in the media. The structural organization and quantity of different types of collagen were found to depend on the artery caliber. The distribution of I, III, IV, V type collagen in the aortal intima is described. The localization of athrombogenic collagen of type IV and V in the area of the endothelial basal membrane is highlighted. The authors revealed the presence of I and III type collagen in the subendothelium and the age-related increase in interstitial collagen levels in the intima which is important for predicting thrombosis in deendothelization . Employing immunoelectron microscopy, previously undescribed forms of I, III, IV, V type collagen organization into microgranular structures were ascertained.
    MeSH term(s) Arteries/metabolism ; Arteries/ultrastructure ; Collagen/metabolism ; Coronary Vessels/metabolism ; Fluorescent Antibody Technique ; Humans ; Kidney/blood supply ; Liver/blood supply ; Microscopy, Electron ; Spleen/blood supply
    Chemical Substances Collagen (9007-34-5)
    Language Russian
    Publishing date 1984-04
    Publishing country Russia (Federation)
    Document type Comparative Study ; English Abstract ; Journal Article
    ZDB-ID 131029-x
    ISSN 0022-9040
    ISSN 0022-9040
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  5. Article: Belki, prochno sviazannye s DNK v uchastkakh ee prikrepleniia k matriksu interfaznogo iadra.

    Razin, S V / Chernokhvostov, V V / Rudin, A V / Zbarskiĭ, I B / Georgiev, G P

    Doklady Akademii nauk SSSR

    1982  Volume 263, Issue 4, Page(s) 1019–1021

    Title translation Proteins tightly bound to DNA at sites of its attachment to the interphase nuclear matrix.
    MeSH term(s) Animals ; Binding Sites ; Cell Nucleus/metabolism ; Chromosomal Proteins, Non-Histone/metabolism ; DNA/analysis ; DNA/metabolism ; Interphase ; L Cells (Cell Line)/metabolism ; Mice ; Protein Binding/drug effects
    Chemical Substances Chromosomal Proteins, Non-Histone ; DNA (9007-49-2)
    Language Russian
    Publishing date 1982
    Publishing country Russia (Federation)
    Document type Journal Article
    ISSN 0002-3264
    ISSN 0002-3264
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  6. Article: Monoclonal antibody to human endothelial cell surface internalization and liposome delivery in cell culture.

    Trubetskaya, O V / Trubetskoy, V S / Domogatsky, S P / Rudin, A V / Popov, N V / Danilov, S M / Nikolayeva, M N / Klibanov, A L / Torchilin, V P

    FEBS letters

    1988  Volume 228, Issue 1, Page(s) 131–134

    Abstract: A monoclonal antibody (mAb), E25, is described that binds to the surface of cultured human endothelial cells. Upon binding E25 is rapidly internalized and digested intracellularly. Selective liposome targeting to the surface of the cells is performed ... ...

    Abstract A monoclonal antibody (mAb), E25, is described that binds to the surface of cultured human endothelial cells. Upon binding E25 is rapidly internalized and digested intracellularly. Selective liposome targeting to the surface of the cells is performed using a biotinylated E25 antibody and an avidin-biotin system. Up to 30% of the cell-adherent liposomal lipid is internalized.
    MeSH term(s) Animals ; Antibodies, Monoclonal/metabolism ; Antibody Affinity ; Antigens, Surface/metabolism ; Cells, Cultured ; Endothelium, Vascular/immunology ; Endothelium, Vascular/metabolism ; Humans ; Immunoglobulin G/metabolism ; Liposomes/metabolism ; Mice
    Chemical Substances Antibodies, Monoclonal ; Antigens, Surface ; Immunoglobulin G ; Liposomes
    Language English
    Publishing date 1988-02-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 212746-5
    ISSN 1873-3468 ; 0014-5793
    ISSN (online) 1873-3468
    ISSN 0014-5793
    DOI 10.1016/0014-5793(88)80601-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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