Artikel ; Online: Temporal and spatial heterogeneity of host response to SARS-CoV-2 pulmonary infection.
2020 Band 11, Heft 1, Seite(n) 6319
Abstract: The relationship of SARS-CoV-2 pulmonary infection and severity of disease is not fully understood. Here we show analysis of autopsy specimens from 24 patients who succumbed to SARS-CoV-2 infection using a combination of different RNA and protein ... ...
| Abstract | The relationship of SARS-CoV-2 pulmonary infection and severity of disease is not fully understood. Here we show analysis of autopsy specimens from 24 patients who succumbed to SARS-CoV-2 infection using a combination of different RNA and protein analytical platforms to characterize inter-patient and intra-patient heterogeneity of pulmonary virus infection. There is a spectrum of high and low virus cases associated with duration of disease. High viral cases have high activation of interferon pathway genes and a predominant M1-like macrophage infiltrate. Low viral cases are more heterogeneous likely reflecting inherent patient differences in the evolution of host response, but there is consistent indication of pulmonary epithelial cell recovery based on napsin A immunohistochemistry and RNA expression of surfactant and mucin genes. Using a digital spatial profiling platform, we find the virus corresponds to distinct spatial expression of interferon response genes demonstrating the intra-pulmonary heterogeneity of SARS-CoV-2 infection. |
|---|---|
| Mesh-Begriff(e) | Adult ; Aged ; Aged, 80 and over ; Aspartic Acid Endopeptidases/metabolism ; Autopsy ; COVID-19/immunology ; COVID-19/metabolism ; Epithelial Cells/metabolism ; Epithelial Cells/pathology ; Epithelial Cells/virology ; Female ; Host Microbial Interactions ; Humans ; Immunity ; Immunohistochemistry ; In Situ Hybridization ; Interferons/genetics ; Interferons/metabolism ; Lung/pathology ; Lung/virology ; Macrophages/immunology ; Male ; Middle Aged ; Mucins/genetics ; Mucins/metabolism ; Surface-Active Agents/metabolism ; Transcriptome ; Viral Load |
| Chemische Substanzen | Mucins ; Surface-Active Agents ; Interferons (9008-11-1) ; Aspartic Acid Endopeptidases (EC 3.4.23.-) ; NAPSA protein, human (EC 3.4.23.-) |
| Sprache | Englisch |
| Erscheinungsdatum | 2020-12-09 |
| Erscheinungsland | England |
| Dokumenttyp | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2553671-0 |
| ISSN | 2041-1723 ; 2041-1723 |
| ISSN (online) | 2041-1723 |
| ISSN | 2041-1723 |
| DOI | 10.1038/s41467-020-20139-7 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Zusatzmaterialien
Kategorien
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.