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  1. Article: Unilateral Pushup-Induced Acute Median Neuritis.

    Ruppel, Alexis M / Lomis, Mitchell J / Schneider, Scarlett E

    Cureus

    2024  Volume 16, Issue 4, Page(s) e57549

    Abstract: Acute median nerve neuropathy is most often due to trauma; acute carpal tunnel syndrome is considered a surgical emergency and must be ruled out. A right-hand dominant male presented to the emergency department with progressive unilateral pain and ... ...

    Abstract Acute median nerve neuropathy is most often due to trauma; acute carpal tunnel syndrome is considered a surgical emergency and must be ruled out. A right-hand dominant male presented to the emergency department with progressive unilateral pain and numbness in the median nerve distribution after experiencing a pop while doing pushups. The evaluation was limited to pain, but there was no gross deformity, and the distal right upper extremity was neurovascularly intact. All imaging was unremarkable. The patient received adequate pain control and complete resolution of symptoms. Despite presenting with symptoms congruent with possible carpal tunnel syndrome, the patient's physical exam and imaging findings were inconsistent with the diagnosis. Acute median nerve neuritis is less commonly described, and no cases have been reported secondary to push-ups, but it should be considered in nontraumatic patients. With conservative management, patients can have complete resolution and no reoccurrence of symptoms.
    Language English
    Publishing date 2024-04-03
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.57549
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans.

    Scholte, Larissa L S / Leggat, David J / Cohen, Kristen W / Hoeweler, Lara / Erwin, Guacyara C / Rahaman, Farhard / Lombardo, Angela / Philiponis, Vincent / Laufer, Dagna S / Siefers, Heather / Ruppel, Alexis M / Brand, Joshua / Maenza, Janine / Bronson, Rhi / Prabhakaran, Madhu / Jean-Baptiste, Jalen / Kolokythas, Orpheus / Desrosiers, Aimee A / Thoreson, Caroline K /
    Heit, Antje / Khati, Nadia J / Malkin, Elissa / McElrath, M Juliana / McDermott, Adrian B / Schief, William R / Diemert, David / Bethony, Jeffrey M

    STAR protocols

    2023  Volume 4, Issue 4, Page(s) 102576

    Abstract: The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations ... ...

    Abstract The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022).
    MeSH term(s) Humans ; Biopsy, Fine-Needle ; Lymph Nodes/diagnostic imaging ; Lymph Nodes/pathology ; Germinal Center ; Vaccination ; Ultrasonography, Interventional
    Language English
    Publishing date 2023-09-20
    Publishing country United States
    Document type Journal Article
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2023.102576
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Human immunoglobulin gene allelic variation impacts germline-targeting vaccine priming.

    deCamp, Allan C / Corcoran, Martin M / Fulp, William J / Willis, Jordan R / Cottrell, Christopher A / Bader, Daniel L V / Kalyuzhniy, Oleksandr / Leggat, David J / Cohen, Kristen W / Hyrien, Ollivier / Menis, Sergey / Finak, Greg / Ballweber-Fleming, Lamar / Srikanth, Abhinaya / Plyler, Jason R / Rahaman, Farhad / Lombardo, Angela / Philiponis, Vincent / Whaley, Rachael E /
    Seese, Aaron / Brand, Joshua / Ruppel, Alexis M / Hoyland, Wesley / Mahoney, Celia R / Cagigi, Alberto / Taylor, Alison / Brown, David M / Ambrozak, David R / Sincomb, Troy / Mullen, Tina-Marie / Maenza, Janine / Kolokythas, Orpheus / Khati, Nadia / Bethony, Jeffrey / Roederer, Mario / Diemert, David / Koup, Richard A / Laufer, Dagna S / McElrath, Juliana M / McDermott, Adrian B / Karlsson Hedestam, Gunilla B / Schief, William R

    NPJ vaccines

    2024  Volume 9, Issue 1, Page(s) 58

    Abstract: Vaccine priming immunogens that activate germline precursors for broadly neutralizing antibodies (bnAbs) have promise for development of precision vaccines against major human pathogens. In a clinical trial of the eOD-GT8 60mer germline-targeting ... ...

    Abstract Vaccine priming immunogens that activate germline precursors for broadly neutralizing antibodies (bnAbs) have promise for development of precision vaccines against major human pathogens. In a clinical trial of the eOD-GT8 60mer germline-targeting immunogen, higher frequencies of vaccine-induced VRC01-class bnAb-precursor B cells were observed in the high dose compared to the low dose group. Through immunoglobulin heavy chain variable (IGHV) genotyping, statistical modeling, quantification of IGHV1-2 allele usage and B cell frequencies in the naive repertoire for each trial participant, and antibody affinity analyses, we found that the difference between dose groups in VRC01-class response frequency was best explained by IGHV1-2 genotype rather than dose and was most likely due to differences in IGHV1-2 B cell frequencies for different genotypes. The results demonstrate the need to define population-level immunoglobulin allelic variations when designing germline-targeting immunogens and evaluating them in clinical trials.
    Language English
    Publishing date 2024-03-11
    Publishing country England
    Document type Journal Article
    ISSN 2059-0105
    ISSN (online) 2059-0105
    DOI 10.1038/s41541-024-00811-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Human immunoglobulin gene allelic variation impacts germline-targeting vaccine priming.

    deCamp, Allan C / Corcoran, Martin M / Fulp, William J / Willis, Jordan R / Cottrell, Christopher A / Bader, Daniel L V / Kalyuzhniy, Oleksandr / Leggat, David J / Cohen, Kristen W / Hyrien, Ollivier / Menis, Sergey / Finak, Greg / Ballweber-Fleming, Lamar / Srikanth, Abhinaya / Plyler, Jason R / Rahaman, Farhad / Lombardo, Angela / Philiponis, Vincent / Whaley, Rachael E /
    Seese, Aaron / Brand, Joshua / Ruppel, Alexis M / Hoyland, Wesley / Mahoney, Celia R / Cagigi, Alberto / Taylor, Alison / Brown, David M / Ambrozak, David R / Sincomb, Troy / Mullen, Tina-Marie / Maenza, Janine / Kolokythas, Orpheus / Khati, Nadia / Bethony, Jeffrey / Roederer, Mario / Diemert, David / Koup, Richard A / Laufer, Dagna S / McElrath, Juliana M / McDermott, Adrian B / Hedestam, Gunilla B Karlsson / Schief, William R

    medRxiv : the preprint server for health sciences

    2023  

    Abstract: Vaccine priming immunogens that activate germline precursors for broadly neutralizing antibodies (bnAbs) have promise for development of precision vaccines against major human pathogens. In a clinical trial of the eOD-GT8 60mer germline-targeting ... ...

    Abstract Vaccine priming immunogens that activate germline precursors for broadly neutralizing antibodies (bnAbs) have promise for development of precision vaccines against major human pathogens. In a clinical trial of the eOD-GT8 60mer germline-targeting immunogen, higher frequencies of vaccine-induced VRC01-class bnAb-precursor B cells were observed in the high dose compared to the low dose group. Through immunoglobulin heavy chain variable (IGHV) genotyping, statistical modeling, quantification of IGHV1-2 allele usage and B cell frequencies in the naive repertoire for each trial participant, and antibody affinity analyses, we found that the difference between dose groups in VRC01-class response frequency was best explained by IGHV1-2 genotype rather than dose and was most likely due to differences in IGHV1-2 B cell frequencies for different genotypes. The results demonstrate the need to define population-level immunoglobulin allelic variations when designing germline-targeting immunogens and evaluating them in clinical trials.
    One-sentence summary: Human genetic variation can modulate the strength of vaccine-induced broadly neutralizing antibody precursor B cell responses.
    Language English
    Publishing date 2023-03-15
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.03.10.23287126
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Vaccination induces HIV broadly neutralizing antibody precursors in humans.

    Leggat, David J / Cohen, Kristen W / Willis, Jordan R / Fulp, William J / deCamp, Allan C / Kalyuzhniy, Oleksandr / Cottrell, Christopher A / Menis, Sergey / Finak, Greg / Ballweber-Fleming, Lamar / Srikanth, Abhinaya / Plyler, Jason R / Schiffner, Torben / Liguori, Alessia / Rahaman, Farhad / Lombardo, Angela / Philiponis, Vincent / Whaley, Rachael E / Seese, Aaron /
    Brand, Joshua / Ruppel, Alexis M / Hoyland, Wesley / Yates, Nicole L / Williams, LaTonya D / Greene, Kelli / Gao, Hongmei / Mahoney, Celia R / Corcoran, Martin M / Cagigi, Alberto / Taylor, Alison / Brown, David M / Ambrozak, David R / Sincomb, Troy / Hu, Xiaozhen / Tingle, Ryan / Georgeson, Erik / Eskandarzadeh, Saman / Alavi, Nushin / Lu, Danny / Mullen, Tina-Marie / Kubitz, Michael / Groschel, Bettina / Maenza, Janine / Kolokythas, Orpheus / Khati, Nadia / Bethony, Jeffrey / Crotty, Shane / Roederer, Mario / Karlsson Hedestam, Gunilla B / Tomaras, Georgia D / Montefiori, David / Diemert, David / Koup, Richard A / Laufer, Dagna S / McElrath, M Juliana / McDermott, Adrian B / Schief, William R

    Science (New York, N.Y.)

    2022  Volume 378, Issue 6623, Page(s) eadd6502

    Abstract: Broadly neutralizing antibodies (bnAbs) can protect against HIV infection but have not been induced by human vaccination. A key barrier to bnAb induction is vaccine priming of rare bnAb-precursor B cells. In a randomized, double-blind, placebo-controlled ...

    Abstract Broadly neutralizing antibodies (bnAbs) can protect against HIV infection but have not been induced by human vaccination. A key barrier to bnAb induction is vaccine priming of rare bnAb-precursor B cells. In a randomized, double-blind, placebo-controlled phase 1 clinical trial, the HIV vaccine-priming candidate eOD-GT8 60mer adjuvanted with AS01
    MeSH term(s) Humans ; Adjuvants, Immunologic ; AIDS Vaccines/immunology ; Broadly Neutralizing Antibodies/genetics ; Broadly Neutralizing Antibodies/immunology ; HIV Infections/prevention & control ; Vaccination ; HIV Antibodies/genetics ; HIV Antibodies/immunology ; Germ Cells/immunology ; B-Lymphocytes/immunology ; Mutation ; Immunoglobulin Light Chains/genetics ; Immunoglobulin Light Chains/immunology ; Immunoglobulin Heavy Chains/genetics ; Immunoglobulin Heavy Chains/immunology ; Male ; Female ; Adult
    Chemical Substances Adjuvants, Immunologic ; AIDS Vaccines ; Broadly Neutralizing Antibodies ; HIV Antibodies ; Immunoglobulin Light Chains ; Immunoglobulin Heavy Chains
    Language English
    Publishing date 2022-12-02
    Publishing country United States
    Document type Clinical Trial, Phase I ; Journal Article ; Randomized Controlled Trial ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 128410-1
    ISSN 1095-9203 ; 0036-8075
    ISSN (online) 1095-9203
    ISSN 0036-8075
    DOI 10.1126/science.add6502
    Database MEDical Literature Analysis and Retrieval System OnLINE

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