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  1. Article ; Online: An ion mobility-mass spectrometry study of copper-metallothionein-2A: binding sites and stabilities of Cu-MT and mixed metal Cu-Ag and Cu-Cd complexes.

    Fan, Liqi / Russell, David H

    The Analyst

    2023  Volume 148, Issue 3, Page(s) 546–555

    Abstract: The presence of Cu, a highly redox active metal, is known to damage DNA as well as other cellular components, but the adverse effects of cellular Cu can be mitigated by metallothioneins (MT), small cysteine rich proteins that are known to bind to a broad ...

    Abstract The presence of Cu, a highly redox active metal, is known to damage DNA as well as other cellular components, but the adverse effects of cellular Cu can be mitigated by metallothioneins (MT), small cysteine rich proteins that are known to bind to a broad range of metal ions. While metal ion binding has been shown to involve the cysteine thiol groups, the specific ion binding sites are controversial as are the overall structure and stability of the Cu-MT complexes. Here, we report results obtained using nano-electrospray ionization mass spectrometry and ion mobility-mass spectrometry for several Cu-MT complexes and compare our results with those previously reported for Ag-MT complexes. The data include determination of the stoichiometries of the complex (Cu
    Language English
    Publishing date 2023-01-31
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d2an01556k
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2423: Show issues Location:
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  2. Article ; Online: Increasing Isolation Efficiency Using a Segmented Quadrupole Mass Filter Operated with Rectangular Waveforms.

    Schrader, Robert L / Anderson, Gordon A / Russell, David H

    Journal of the American Society for Mass Spectrometry

    2024  

    Abstract: The performance of a segmented quadrupole mass filter operated with rectangular waveforms and capacitively coupled rectangular waveforms applied to the prefilters was examined on a home-built quadrupole-Orbitrap platform. For peak widths of ... ...

    Abstract The performance of a segmented quadrupole mass filter operated with rectangular waveforms and capacitively coupled rectangular waveforms applied to the prefilters was examined on a home-built quadrupole-Orbitrap platform. For peak widths of 50
    Language English
    Publishing date 2024-04-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1073671-2
    ISSN 1879-1123 ; 1044-0305
    ISSN (online) 1879-1123
    ISSN 1044-0305
    DOI 10.1021/jasms.4c00051
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4618: Show issues Location:
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    Zs.MO 241: Show issues

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  3. Article ; Online: Modified Ion Source for the Improved Collisional Activation of Protein Complexes.

    Schrader, Robert L / Walker, Thomas E / Russell, David H

    Journal of the American Society for Mass Spectrometry

    2023  Volume 34, Issue 5, Page(s) 977–980

    Abstract: The analysis of large molecules is challenging, as they often have salts and adducts retained through the electrospray process, which increase the observed mass and compromise the achievable mass resolution. Mild collisional activation has been shown to ... ...

    Abstract The analysis of large molecules is challenging, as they often have salts and adducts retained through the electrospray process, which increase the observed mass and compromise the achievable mass resolution. Mild collisional activation has been shown to be very effective for the removal of adducts and increases both measurement accuracy and mass resolution of large (>100 kDa) protein complexes. Collisionally activated protein ions are more completely desolvated due to the increased number of collisions when trapped following activation. A short square quadrupole maintained at 300 mTorr by a mechanical pump was added between the ion funnel and transmission quadrupole. This configuration and operation effectively removed adducts from the 800 kDa tetradecamer GroEL as well as fragmented smaller protein complexes like C-reactive protein. Due to the gas high pressure, ions of low size-to-charge ratio, such as those in charge reducing buffers, had low ejection efficiency. We show that segmenting the quadrupole rods greatly improves signal intensity for charge reduced GroEL D398A mutant compared to nonsegmented rods when operating at high pressure.
    MeSH term(s) Proteins/chemistry ; Ions/chemistry ; Mass Spectrometry/instrumentation
    Chemical Substances Proteins ; Ions
    Language English
    Publishing date 2023-03-31
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1073671-2
    ISSN 1879-1123 ; 1044-0305
    ISSN (online) 1879-1123
    ISSN 1044-0305
    DOI 10.1021/jasms.3c00071
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Editorial: Focus on Ionization Technologies Used in MS: Fundamentals and Applications, Honoring Dr. Sarah Trimpin, Recipient of the 2019 ASMS Biemann Medal.

    Russell, David H / Clemmer, David E

    Journal of the American Society for Mass Spectrometry

    2021  Volume 32, Issue 3, Page(s) 616–617

    Language English
    Publishing date 2021-06-23
    Publishing country United States
    Document type Editorial
    ZDB-ID 1073671-2
    ISSN 1879-1123 ; 1044-0305
    ISSN (online) 1879-1123
    ISSN 1044-0305
    DOI 10.1021/jasms.1c00030
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Double and triple thermodynamic mutant cycles reveal the basis for specific MsbA-lipid interactions.

    Lyu, Jixing / Zhang, Tianqi / Marty, Michael T / Clemmer, David / Russell, David H / Laganowsky, Arthur

    eLife

    2024  Volume 12

    Abstract: Structural and functional studies of the ATP-binding cassette transporter MsbA have revealed two distinct lipopolysaccharide (LPS) binding sites: one located in the central cavity and the other at a membrane-facing, exterior site. Although these binding ... ...

    Abstract Structural and functional studies of the ATP-binding cassette transporter MsbA have revealed two distinct lipopolysaccharide (LPS) binding sites: one located in the central cavity and the other at a membrane-facing, exterior site. Although these binding sites are known to be important for MsbA function, the thermodynamic basis for these specific MsbA-LPS interactions is not well understood. Here, we use native mass spectrometry to determine the thermodynamics of MsbA interacting with the LPS-precursor 3-deoxy-D-
    MeSH term(s) Lipopolysaccharides ; Thermodynamics ; Entropy ; ATP-Binding Cassette Transporters ; Binding Sites
    Chemical Substances Lipopolysaccharides ; ATP-Binding Cassette Transporters
    Language English
    Publishing date 2024-01-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2687154-3
    ISSN 2050-084X ; 2050-084X
    ISSN (online) 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.91094
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  6. Article ; Online: Surface Activity of Amines Provides Evidence for the Combined ESI Mechanism of Charge Reduction for Protein Complexes.

    Walker, Thomas E / Laganowsky, Arthur / Russell, David H

    Analytical chemistry

    2022  Volume 94, Issue 30, Page(s) 10824–10831

    Abstract: Charge reduction reactions are important for native mass spectrometry (nMS) because lower charge states help retain native-like conformations and preserve noncovalent interactions of protein complexes. While mechanisms of charge reduction reactions are ... ...

    Abstract Charge reduction reactions are important for native mass spectrometry (nMS) because lower charge states help retain native-like conformations and preserve noncovalent interactions of protein complexes. While mechanisms of charge reduction reactions are not well understood, they are generally achieved through the addition of small molecules, such as polyamines, to traditional nMS buffers. Here, we present new evidence that surface-active, charge reducing reagents carry away excess charge from the droplet after being emitted due to Coulombic repulsion, thereby reducing the overall charge of the droplet. Furthermore, these processes are directly linked to two mechanisms for electrospray ionization, specifically the charge residue and ion evaporation models (CRM and IEM). Selected protein complexes were analyzed in solutions containing ammonium acetate and selected trialkylamines or diaminoalkanes of increasing alkyl chain lengths. Results show that amines with higher surface activity have increased propensities for promoting charge reduction of the protein ions. The electrospray ionization (ESI) emitter potential was also found to be a major contributing parameter to the prevalence of charge reduction; higher emitter potentials consistently coincided with lower average charge states among all protein complexes analyzed. These results offer experimental evidence for the mechanism of charge reduction in ESI and also provide insight into the final stages of the ESI and their impact on biological ions.
    MeSH term(s) Amines ; Ions/chemistry ; Proteins/chemistry ; Spectrometry, Mass, Electrospray Ionization/methods
    Chemical Substances Amines ; Ions ; Proteins
    Language English
    Publishing date 2022-07-21
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c01814
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
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  7. Article: Surface Activity of Amines Provides Evidence for the Combined ESI Mechanism of Charge Reduction for Protein Complexes

    Walker, Thomas E. / Laganowsky, Arthur / Russell, David H.

    Analytical chemistry. 2022 July 21, v. 94, no. 30

    2022  

    Abstract: Charge reduction reactions are important for native mass spectrometry (nMS) because lower charge states help retain native-like conformations and preserve noncovalent interactions of protein complexes. While mechanisms of charge reduction reactions are ... ...

    Abstract Charge reduction reactions are important for native mass spectrometry (nMS) because lower charge states help retain native-like conformations and preserve noncovalent interactions of protein complexes. While mechanisms of charge reduction reactions are not well understood, they are generally achieved through the addition of small molecules, such as polyamines, to traditional nMS buffers. Here, we present new evidence that surface-active, charge reducing reagents carry away excess charge from the droplet after being emitted due to Coulombic repulsion, thereby reducing the overall charge of the droplet. Furthermore, these processes are directly linked to two mechanisms for electrospray ionization, specifically the charge residue and ion evaporation models (CRM and IEM). Selected protein complexes were analyzed in solutions containing ammonium acetate and selected trialkylamines or diaminoalkanes of increasing alkyl chain lengths. Results show that amines with higher surface activity have increased propensities for promoting charge reduction of the protein ions. The electrospray ionization (ESI) emitter potential was also found to be a major contributing parameter to the prevalence of charge reduction; higher emitter potentials consistently coincided with lower average charge states among all protein complexes analyzed. These results offer experimental evidence for the mechanism of charge reduction in ESI and also provide insight into the final stages of the ESI and their impact on biological ions.
    Keywords ammonium acetate ; analytical chemistry ; droplets ; electrospray ionization mass spectrometry ; evaporation ; polyamines
    Language English
    Dates of publication 2022-0721
    Size p. 10824-10831.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c01814
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Tracking the Structural Evolution of 4-Aminobenzoic Acid in the Transition from Solution to the Gas Phase.

    Hebert, Michael J / Russell, David H

    The journal of physical chemistry. B

    2020  Volume 124, Issue 11, Page(s) 2081–2087

    Abstract: Here, cryogenic ion mobility-mass spectrometry (cryo-IM-MS) is used to investigate intracluster proton transfer reactions of 4-aminobenzoic acid during the transition from solution to the gas phase. Previous studies have shown that protonation of the ... ...

    Abstract Here, cryogenic ion mobility-mass spectrometry (cryo-IM-MS) is used to investigate intracluster proton transfer reactions of 4-aminobenzoic acid during the transition from solution to the gas phase. Previous studies have shown that protonation of the amine group of 4-aminobenzoic acid (4-ABAH
    Language English
    Publishing date 2020-03-06
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ISSN 1520-5207
    ISSN (online) 1520-5207
    DOI 10.1021/acs.jpcb.9b10576
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Characterization of lipid carbon-carbon double-bond isomerism via ion mobility-mass spectrometry (IMS-MS) combined with cuprous ion-induced fragmentation.

    Kuo, Syuan-Ting / Tang, Shuli / Russell, David H / Yan, Xin

    International journal of mass spectrometry

    2022  Volume 479

    Abstract: Characterization of phospholipid isomers is challenging due to their identical masses and similarities in structures. Here, we report that copper (I) ion complexed with phospholipids can be used to characterize both carbon-carbon double-bond (C=C bond) ... ...

    Abstract Characterization of phospholipid isomers is challenging due to their identical masses and similarities in structures. Here, we report that copper (I) ion complexed with phospholipids can be used to characterize both carbon-carbon double-bond (C=C bond) positional and geometric isomers. We investigate the distinct fragmentation patterns induced by the π-Cu
    Language English
    Publishing date 2022-06-18
    Publishing country Netherlands
    Document type Journal Article
    ISSN 1387-3806
    ISSN 1387-3806
    DOI 10.1016/j.ijms.2022.116889
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  10. Article ; Online: Editorial: Focus on Protein Footprinting, Honoring Michael Gross, Recipient of the 2020 John B. Fenn Award for a Distinguished Contribution in Mass Spectrometry.

    Loo, Joseph A / Russell, David H / Wang, Yinsheng

    Journal of the American Society for Mass Spectrometry

    2021  Volume 32, Issue 7, Page(s) 1565–1566

    Language English
    Publishing date 2021-07-13
    Publishing country United States
    Document type Editorial
    ZDB-ID 1073671-2
    ISSN 1879-1123 ; 1044-0305
    ISSN (online) 1879-1123
    ISSN 1044-0305
    DOI 10.1021/jasms.1c00197
    Database MEDical Literature Analysis and Retrieval System OnLINE

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