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  1. Article: Gallstone Ileus Post-cholecystectomy: A Case Review.

    Helmy, Nader A / Ryska, Ondrej

    Cureus

    2023  Volume 15, Issue 1, Page(s) e33345

    Abstract: Gallstone ileus is an unusual cause of small bowel obstruction, in general, let alone after cholecystectomy. It occurs in patients with chronic calculous cholecystitis and gallstones who develop a cholecystoduodenal fistula over time. The diagnosis is ... ...

    Abstract Gallstone ileus is an unusual cause of small bowel obstruction, in general, let alone after cholecystectomy. It occurs in patients with chronic calculous cholecystitis and gallstones who develop a cholecystoduodenal fistula over time. The diagnosis is made based on clinical presentation and examination findings and is confirmed with the use of radiological modalities, such as computed tomography (CT) scan, which has been proven to be the most sensitive investigation in diagnosis. Here, we present a case of gallstone ileus that occurred 25 years after laparoscopic cholecystectomy. CT scan on admission showed adhesional small bowel obstruction given the patient's previous abdominal surgery. The patient was managed conservatively as per guidelines for the management of adhesional small bowel obstruction for 72 hours. Obstructive symptoms did not resolve despite all conservative measures, and a gastrografin challenge showed no contrast reaching the colon. Hence, the patient underwent an exploratory laparotomy to manage his ongoing bowel obstruction. Laparotomy revealed gallstone ileus as the cause of obstruction. This case highlights the importance of considering gallstone ileus in the differential diagnosis for patients who present to the emergency department with small bowel obstruction even years after cholecystectomy. Post-cholecystectomy gallstone ileus is very rare with very few cases reported in the literature. This condition poses diagnostic challenges both because of its rarity and because the gallbladder had been previously removed. A high index of suspicion by the surgeon is needed for diagnosis.
    Language English
    Publishing date 2023-01-04
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.33345
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Surface Microdialysis for Detection of Colorectal Anastomosis Ischemia-An Experimental Study.

    Ryska, Ondrej / Kalvach, Jaroslav / Pazin, Jaroslav / Hadac, Jan / Martinek, Jan / Juhas, Stefan / Juhasova, Jana

    The Journal of surgical research

    2021  Volume 261, Page(s) 293–300

    Abstract: Background: Inadequate blood supply is one of the major risk factors for anastomotic leak after low anterior rectal resection. Early detection of ischemia might predict complicated healing and enable anastomotic salvage, which is associated with better ... ...

    Abstract Background: Inadequate blood supply is one of the major risk factors for anastomotic leak after low anterior rectal resection. Early detection of ischemia might predict complicated healing and enable anastomotic salvage, which is associated with better outcomes. A microdialysis offers a real-time evaluation of adequate bowel perfusion through monitoring of tissue metabolism. In this experimental study, we assessed the role of microdialysis in detecting early ischemia after colorectal anastomosis.
    Materials and methods: Colorectal anastomosis was performed in six miniature pigs. A microdialysis catheter was placed on the bowel serosa 5 mm proximal to the anastomosis. Metabolic changes were monitored hourly before and after initiating ischemia, which was induced by ligation of the inferior mesenteric artery and skeletonization of the mesocolon.
    Results: Significant increase in tissue levels of lactate was detected after 60 min of ischemia (13.6 [10.4-16.1] versus 6.75 [1.8-9.6] mmol/L at baseline; P < 0.005). The lactate/pyruvate ratio increased accordingly. The concentration of glycerol increased significantly after 2 hours-from a baseline value of 29.5 (3-84) to 125 (79-201) mmol/L (P < 0.005). The decrease in glucose levels was also significant after 60 minutes-0 (0-0.2) versus 4.7 (3.3-6.8) mmol/L at baseline. However, its values started to decline before ischemia.
    Conclusions: Surface microdialysis can detect ischemic changes early and may be a promising method in postoperative monitoring of colorectal anastomosis.
    MeSH term(s) Anastomosis, Surgical/adverse effects ; Animals ; Blood Glucose/analysis ; Female ; Glycerol/metabolism ; Ischemia/diagnosis ; Ischemia/etiology ; Ischemia/metabolism ; Lactic Acid/metabolism ; Male ; Microdialysis/methods ; Postoperative Complications/diagnosis ; Postoperative Complications/etiology ; Postoperative Complications/metabolism ; Swine ; Swine, Miniature
    Chemical Substances Blood Glucose ; Lactic Acid (33X04XA5AT) ; Glycerol (PDC6A3C0OX)
    Language English
    Publishing date 2021-01-18
    Publishing country United States
    Document type Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80170-7
    ISSN 1095-8673 ; 0022-4804
    ISSN (online) 1095-8673
    ISSN 0022-4804
    DOI 10.1016/j.jss.2020.12.021
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  3. Article ; Online: A Randomized Comparison of Bougie-Assisted and TracheoQuick Plus Cricothyrotomies on a Live Porcine Model.

    Henlin, Tomas / Michalek, Pavel / Tyll, Tomas / Ryska, Ondrej

    BioMed research international

    2017  Volume 2017, Page(s) 4215159

    Abstract: Objectives: Cricothyrotomy is a rescue procedure in "cannot intubate, cannot oxygenate" scenarios where other methods of nonsurgical airway management have failed. We compared 2 cuffed cricothyrotomy sets, bougie-assisted cricothyrotomy (BACT) and novel ...

    Abstract Objectives: Cricothyrotomy is a rescue procedure in "cannot intubate, cannot oxygenate" scenarios where other methods of nonsurgical airway management have failed. We compared 2 cuffed cricothyrotomy sets, bougie-assisted cricothyrotomy (BACT) and novel percutaneous TracheoQuick Plus, on a live porcine model in a simulated periarrest situation.
    Methods: Thirty-four anesthetized minipigs were randomly allocated into two groups: BACT technique (
    Results: BACT was significantly faster than TracheoQuick Plus cricothyrotomy, with a median time of 69 sec (IQR 56-85) versus 178 sec (IQR 152-272). The total success rate was without difference. 94% of BACT was performed successfully on the first attempt, while in the TracheoQuick Plus group, it was only 18% (
    Conclusions: BACT is superior to TracheoQuick Plus cricothyrotomy on a live animal model.
    MeSH term(s) Animals ; Cricoid Cartilage/surgery ; Heart Rate/physiology ; Intubation, Intratracheal/adverse effects ; Intubation, Intratracheal/instrumentation ; Intubation, Intratracheal/methods ; Intubation, Intratracheal/statistics & numerical data ; Models, Animal ; Oxygen/blood ; Random Allocation ; Swine ; Swine, Miniature ; Time Factors ; Tracheotomy/adverse effects ; Tracheotomy/instrumentation ; Tracheotomy/methods ; Tracheotomy/statistics & numerical data
    Chemical Substances Oxygen (S88TT14065)
    Language English
    Publishing date 2017-12-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2698540-8
    ISSN 2314-6141 ; 2314-6133
    ISSN (online) 2314-6141
    ISSN 2314-6133
    DOI 10.1155/2017/4215159
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  4. Article ; Online: Periprocedural safety profile of peroral endoscopic myotomy (POEM)-a retrospective analysis of adverse events according to two different classifications.

    Simkova, Dagmar / Mares, Jan / Vackova, Zuzana / Hucl, Tomas / Stirand, Petr / Kieslichova, Eva / Ryska, Ondrej / Spicak, Julius / Drazilova, Sylvia / Veseliny, Eduard / Martinek, Jan

    Surgical endoscopy

    2022  Volume 37, Issue 2, Page(s) 1242–1251

    Abstract: Background: Peroral endoscopic myotomy (POEM) is nowadays a standard method for treatment of achalasia; nevertheless, it remains an invasive intervention with corresponding risk of adverse events (AEs). The classification and grading of AEs are still a ... ...

    Abstract Background: Peroral endoscopic myotomy (POEM) is nowadays a standard method for treatment of achalasia; nevertheless, it remains an invasive intervention with corresponding risk of adverse events (AEs). The classification and grading of AEs are still a matter of discussion. The aim of our retrospective study was to assess the occurrence of all "undesirable" events and "true" adverse events in patients undergoing POEM and to compare the outcomes when either Clavien-Dindo classification (CDC) or American Society of Gastrointestinal Endoscopy (ASGE) lexicon classification applied.
    Methods: This was a retrospective analysis of prospectively managed database of all patients who had undergone POEM between December 2012 and August 2018. We assessed the pre-, peri-, and early-postoperative (up to patient's discharge) undesirable events (including those not fulfilling criteria for AEs) and "true" AEs according the definition in either of the classifications.
    Results: A total of 231 patients have successfully undergone 244 POEM procedures (13 × re-POEM). Twenty-nine procedures (11.9%) passed uneventfully, while in 215 procedures (88.1%), a total of 440 undesirable events occurred. The CDC identified 27 AEs (17 minor, 10 major) occurring in 23/244 (9.4%) procedures. The ASGE lexicon identified identical 27 AEs (21 mild or moderate, 6 severe or fatal) resulting in the severity distribution of AEs being the only difference between the two classifications. Only the absence of previous treatment was found to be a risk factor [p = 0.047, OR with 95% CI: 4.55 (1.02; 20.25)] in the combined logistic regression model.
    Conclusion: Undesirable events are common in patients undergoing POEM but the incidence of true AEs is low according to both classifications. Severe adverse events are infrequent irrespective of the classification applied. CDC may be more appropriate than ASGE lexicon for classifying POEM-related AEs given a surgical nature of this procedure.
    MeSH term(s) Humans ; Retrospective Studies ; Esophageal Achalasia/surgery ; Digestive System Surgical Procedures ; Risk Factors ; Myotomy/methods ; Natural Orifice Endoscopic Surgery/methods ; Treatment Outcome ; Esophageal Sphincter, Lower/surgery
    Language English
    Publishing date 2022-09-28
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 639039-0
    ISSN 1432-2218 ; 0930-2794
    ISSN (online) 1432-2218
    ISSN 0930-2794
    DOI 10.1007/s00464-022-09621-z
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  5. Article ; Online: A novel postgraduate endoscopic course using a large animal model of secondary Crohn's disease stricture.

    Lukas, Martin / Kolar, Martin / Ryska, Ondrej / Juhas, Stefan / Juhasova, Jana / Kalvach, Jaroslav / Pazin, Jaroslav / Kocisova, Tereza / Foltan, Ondrej / Kristianova, Hana / Ptacnik, Jan / Vitkova, Ivana / Bortlik, Martin / Lukas, Milan

    Surgical endoscopy

    2021  Volume 35, Issue 6, Page(s) 3199–3204

    Abstract: Background: The increasing complexity of advanced endoscopic techniques places a high demand on the endoscopist's expertise. Thus, live porcine models have been more frequently used for training. We briefly describe a hands-on postgraduate endoscopic ... ...

    Abstract Background: The increasing complexity of advanced endoscopic techniques places a high demand on the endoscopist's expertise. Thus, live porcine models have been more frequently used for training. We briefly describe a hands-on postgraduate endoscopic course regarding a novel method of treatment of anastomotic strictures in a porcine model.
    Methods: The porcine model of Crohn's disease anastomotic stricture with two artificial side-to-side ileo-colonic anastomoses was used. Participants performed endoscopic stricturotomy under supervision at one of two equipped endoscopic stations. Available animals were endoscopically re-examined 3 months after the course.
    Results: Twelve anastomoses were prepared for the course. Eleven circumferential stricturotomies together with horizontal cut and clip placement were conducted. All anastomoses were passable for the scope after the procedure, and no case of perforation or bleeding occurred. All anastomoses available for re-examination remained passable for the endoscope after 3 months.
    Conclusion: We successfully organised the first endoscopic hands-on course for the training of endoscopic stricturotomy on a large animal model.
    MeSH term(s) Animals ; Constriction, Pathologic/etiology ; Constriction, Pathologic/surgery ; Crohn Disease/complications ; Crohn Disease/surgery ; Dilatation ; Disease Models, Animal ; Humans ; Swine ; Treatment Outcome
    Language English
    Publishing date 2021-03-04
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639039-0
    ISSN 1432-2218 ; 0930-2794
    ISSN (online) 1432-2218
    ISSN 0930-2794
    DOI 10.1007/s00464-021-08360-x
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  6. Article ; Online: Local application of adipose-derived mesenchymal stem cells supports the healing of fistula: prospective randomised study on rat model of fistulising Crohn's disease.

    Ryska, Ondrej / Serclova, Zuzana / Mestak, Ondrej / Matouskova, Eva / Vesely, Pavel / Mrazova, Iveta

    Scandinavian journal of gastroenterology

    2017  Volume 52, Issue 5, Page(s) 543–550

    Abstract: Objective: Local application of adipose-derived mesenchymal stem cells (ADSC) represents a novel approach for the management of perianal fistula in patients with Crohn's disease. A randomised study on an animal model was performed to investigate the ... ...

    Abstract Objective: Local application of adipose-derived mesenchymal stem cells (ADSC) represents a novel approach for the management of perianal fistula in patients with Crohn's disease. A randomised study on an animal model was performed to investigate the efficacy and to detect the distribution of implanted ADSCs by bioluminescence (BLI).
    Materials and methods: A caecostomy was used as a fistula model in 32 Lewis rats. The ADSCs were isolated from transgenic donor expressing firefly luciferase. Animals were randomly assigned to groups given injections of 4 × 10
    Results: The fistula was identified as healed in 6 (38%) animals in group A vs. 1 case (6.3%) in group B (p = .033). The BLI was strongest immediately after administration of ADSCs 31.2 × 10
    Conclusions: Local application of ADSCs resulted in significantly higher fistula closure rate on an animal model. BLI monitoring was proved to be feasible and showed rapid reduction of the ADSC mass after application. More viable cells were detected in animals with healed fistula at the end of the follow-up.
    MeSH term(s) Adipose Tissue/cytology ; Animals ; Crohn Disease/complications ; Crohn Disease/pathology ; Disease Models, Animal ; Luminescent Measurements ; Male ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells/cytology ; Prospective Studies ; Random Allocation ; Rats ; Rats, Inbred Lew ; Rectal Fistula/therapy ; Regenerative Medicine/methods ; Treatment Outcome ; Wound Healing
    Language English
    Publishing date 2017-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 82042-8
    ISSN 1502-7708 ; 0036-5521
    ISSN (online) 1502-7708
    ISSN 0036-5521
    DOI 10.1080/00365521.2017.1281434
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  7. Article ; Online: Novel porcine model of Crohn's disease anastomotic stricture suitable for evaluation and training of advanced endoscopic techniques.

    Lukas, Martin / Kolar, Martin / Ryska, Ondrej / Juhas, Stefan / Juhasova, Jana / Kalvach, Jaroslav / Pazin, Jaroslav / Kocisova, Tereza / Foltan, Ondrej / Kristianova, Hana / Ptacnik, Jan / Vitkova, Ivana / Bortlik, Martin / Lukas, Milan

    Gastrointestinal endoscopy

    2020  Volume 93, Issue 1, Page(s) 250–256

    Abstract: Background and aims: Currently, treatment options in postsurgical recurrence of stricturing Crohn's disease (CD) are limited. However, development of new invasive endoscopic techniques in clinical practice has safety constraints. The aim of this study ... ...

    Abstract Background and aims: Currently, treatment options in postsurgical recurrence of stricturing Crohn's disease (CD) are limited. However, development of new invasive endoscopic techniques in clinical practice has safety constraints. The aim of this study was to create a large animal model of anastomotic stricture with CD properties to enable development of new techniques and training.
    Methods: A side-to-side ileocolonic anastomosis was created in a modified Roux-en-Y manner with bowel continuity preserved. Two weeks after surgery, we began endoscopic submucosal injections of phenol/trinitrobenzenesulfonic acid solution. This solution was injected every 2 weeks in each quadrant of the anastomosis until development of a stricture. The anastomosis site was assessed endoscopically 2 weeks after the last application (baseline) and then every 2 months until month 6. Endoscopically nonpassable strictures were treated with balloon dilation, endoscopic stricturotomy, and stent placement to confirm the feasibility of such interventions.
    Results: Nineteen minipigs were included with no postoperative adverse events. After a mean of 4.4 ± .7 injection sessions with 10.5 ± 3.0 mL of the solution, anastomotic strictures were created in 16 pigs (84.2%). Mean diameter of the strictures at baseline was 11.6 ± 2.2 mm. The strictures were inflamed, and the endoscope could not pass. Follow-up was successfully completed in 15 animals (79.0%) with the mean deviation from the initial diameter in every measurement of -.02 ± 2.26 mm (P = .963) and a mean final diameter of 11.7 ± 3.4 mm. The histopathologic evaluation revealed the presence of submucosal fibrosis, chronic inflammation, and microgranulomas. All strictures were amenable to endoscopic therapeutic interventions.
    Conclusions: We developed a novel, reproducible porcine model of anastomotic stricture with histologically verified changes mimicking CD and stable diameter for more than 6 months. It is suitable for further endoscopic interventions.
    MeSH term(s) Animals ; Constriction, Pathologic/etiology ; Crohn Disease/surgery ; Dilatation ; Endoscopy ; Humans ; Retrospective Studies ; Swine ; Swine, Miniature ; Treatment Outcome
    Language English
    Publishing date 2020-06-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 391583-9
    ISSN 1097-6779 ; 0016-5107
    ISSN (online) 1097-6779
    ISSN 0016-5107
    DOI 10.1016/j.gie.2020.05.063
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  8. Article ; Online: A new experimental model of calculous cholecystitis suitable for the evaluation and training of minimally invasive approaches to cholecystectomy.

    Ryska, Ondrej / Serclova, Zuzana / Martinek, Jan / Dolezel, Radek / Kalvach, Jaroslav / Juhas, Stefan / Juhasova, Jana / Bunganic, Bohus / Laszikova, Eva / Ryska, Miroslav

    Surgical endoscopy

    2017  Volume 31, Issue 2, Page(s) 987–994

    Abstract: Background: Novel, less invasive approaches such as single-incision laparoscopic cholecystectomy or natural orifice transluminal endoscopic surgery require preclinical evaluation and training. Therefore, there is a need for an experimental model closely ...

    Abstract Background: Novel, less invasive approaches such as single-incision laparoscopic cholecystectomy or natural orifice transluminal endoscopic surgery require preclinical evaluation and training. Therefore, there is a need for an experimental model closely mimicking the clinical situation. The aim of our study was to create an experimental model of calculous cholecystitis in a large laboratory animal and test its feasibility for the evaluation of different techniques of cholecystectomy.
    Methods: In 11 laboratory pigs, gallstones were placed inside the gallbladder laparoscopically. Levels of inflammatory markers-leucocytes (WBC), C-reactive protein (CRP) and interleukin 6 (IL-6)-were monitored on the postoperative days (POD) 1, 2, 3, 7 and 30. Abdominal ultrasound was performed 2 and 4 weeks after the operation. Four weeks after the lithiasis induction, laparoscopic cholecystectomy was performed. The control group consisted of ten healthy animals in which a cholecystectomy was performed. The pigs were monitored for 30 days after surgery. All removed gallbladders were assessed histologically.
    Results: The induction of lithiasis took 42 (35-52) min with no morbidity and mortality. The values of WBC, CRP and IL-6 increased significantly (vs. baseline) on POD 1, 2 and 3 (p < 0.05) and then normalised. Ultrasonography confirmed the presence of chronic calculous cholecystitis in all cases after 4 weeks. Laparoscopic cholecystectomy was significantly longer in animals with lithiasis, 63 (42-91) versus 46 (31-62) min (p = 0.018). Perioperative gallbladder wall perforation was significantly more frequent in the model group (8/11 vs. 1/10; p = 0.04). In contrast to healthy animals, all gallbladders with stones showed histological signs of chronic inflammation.
    Conclusions: A new animal model of calculous cholecystitis was created. Laparoscopic cholecystectomy was more technically difficult compared to operating on a healthy gallbladder. This model may be a suitable tool for effective preclinical training and also for the evaluation of different techniques of cholecystectomy.
    MeSH term(s) Animals ; Cholecystectomy, Laparoscopic/education ; Cholecystectomy, Laparoscopic/methods ; Cholecystolithiasis/surgery ; Disease Models, Animal ; Minimally Invasive Surgical Procedures ; Natural Orifice Endoscopic Surgery/education ; Natural Orifice Endoscopic Surgery/methods ; Swine ; Treatment Outcome
    Language English
    Publishing date 2017-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 639039-0
    ISSN 1432-2218 ; 0930-2794
    ISSN (online) 1432-2218
    ISSN 0930-2794
    DOI 10.1007/s00464-016-5061-0
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  9. Article: Fractionated plasmatic separation and adsorption does not alter haemodynamic parameters in experimental acute liver failure.

    Laszikova, Eva / Prazak, Josef / Ryska, Ondrej / Koblihova, Eva / Tyll, Tomas / Ryska, Miroslav

    Neuro endocrinology letters

    2014  Volume 35, Issue 4, Page(s) 280–284

    Abstract: Objective: Acute liver failure (ALF) is a rare disease with a bad prognosis. Its start is accompanied by haemodynamic instability. The aim of our study was to evaluate the influence of fractionated plasmatic separation and adsorption (FPSA) on body ... ...

    Abstract Objective: Acute liver failure (ALF) is a rare disease with a bad prognosis. Its start is accompanied by haemodynamic instability. The aim of our study was to evaluate the influence of fractionated plasmatic separation and adsorption (FPSA) on body haemodynamics using a large animal experimental model of ALF.
    Methods: ALF was induced by the devascularisation of 21 laboratory pigs. FPSA was applied in 14 animals and seven animals formed a control group. Values of systemic vascular resistance index (SVRI), heart rate (HR), pulmonary artery wedge pressure (PAWP) and cardiac index (CI) at hours 3, 6, 9 and 12 of the experiment were compared. The values from laboratory tests conducted with FPSA-treated vs. untreated ALF animals were compared using Student's t-test, paired or unpaired, as required, and Mann-Whitney U-test using EXCEL and QUATRO spreadsheet applications.
    Results: We found no significant differences in mean arterial pressure, SVRI, or plasma lactate (p>0.05) in the FPSA-treated group but there was a significant decrease(p<0.05) in intracranial pressure (ICP). Furthermore, we observed a significant decrease in HR at hour 3. A significant increase in CI at hour 9 and a significant decrease in pulmonary artery wedge pressure at hours 6 and 12 were also observed.
    Conclusion: Our study of FPSA application (Prometheus device) for treatment of experimental ALF in a large animal model did not confirm the earlier reported development of changes in body haemodynamics.
    MeSH term(s) Animals ; Disease Models, Animal ; Hemodiafiltration/adverse effects ; Hemodiafiltration/instrumentation ; Hemodiafiltration/methods ; Hemodynamics/physiology ; Liver Failure, Acute/physiopathology ; Liver Failure, Acute/therapy ; Swine
    Language English
    Publishing date 2014
    Publishing country Sweden
    Document type Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 135951-4
    ISSN 0172-780X
    ISSN 0172-780X
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  10. Article ; Online: Cerebral microdialysis reflects the neuroprotective effect of fractionated plasma separation and adsorption in acute liver failure better and earlier than intracranial pressure: a controlled study in pigs.

    Prazak, Josef / Laszikova, Eva / Pantoflicek, Tomas / Ryska, Ondrej / Koblihova, Eva / Ryska, Miroslav

    BMC gastroenterology

    2013  Volume 13, Page(s) 98

    Abstract: Background: Cerebral edema is a well-recognized and potentially fatal complication of acute liver failure (ALF). The effectiveness of treatments that address intracranial hypertension is generally assessed by measuring intracranial pressure (ICP). The ... ...

    Abstract Background: Cerebral edema is a well-recognized and potentially fatal complication of acute liver failure (ALF). The effectiveness of treatments that address intracranial hypertension is generally assessed by measuring intracranial pressure (ICP). The aim of this study was to determine the role of cerebral microdialysis in monitoring the efficacy of fractionated plasma separation and adsorption (FPSA) treatment for ALF. We hypothesized that in ALF cerebral microdialysis reflects the benefits of FPSA treatment on cerebral edema before ICP.
    Methods: A surgical resection model of ALF was used in 21 pigs. We measured plasma ammonia concentration, brain concentrations of glucose, lactate, pyruvate, glutamate and glutamine, and ICP. Animals were randomized into three groups: in one group eight animals received 6 hours of FPSA treatment 2 hours after induction of ALF; in another group 10 animals received supportive treatment for ALF only; and in the final group three underwent sham surgery.
    Results: The ICP was significantly higher in the ALF group than in the FPSA group 9 hours after surgery. The lactate/pyruvate (L/P) ratio was significantly lower in the FPSA group than the ALF group 5 hours after surgery, before any significant difference in ICP was detected. Indeed, significant changes in the L/P ratio could be observed within 1 hour of treatment. Glutamine levels were significantly lower in the FPSA group than the ALF group between 6 hours and 10 hours after surgery.
    Conclusions: Brain lactate/pyruvate ratio and concentration of glutamine measured by cerebral microdialysis reflected the beneficial effects of FPSA treatment on cerebral metabolism more precisely and rapidly than ICP in pigs with fulminant ALF. The role of glutamine as a marker of the efficacy of FPSA treatment for ALF appears promising, but needs further evaluation.
    MeSH term(s) Ammonia/blood ; Animals ; Brain Edema/etiology ; Brain Edema/metabolism ; Brain Edema/prevention & control ; Cerebrum/metabolism ; Extracorporeal Circulation ; Glucose/metabolism ; Glutamic Acid/metabolism ; Glutamine/metabolism ; Intracranial Hypertension/etiology ; Intracranial Hypertension/prevention & control ; Intracranial Pressure ; Lactic Acid/metabolism ; Liver Failure, Acute/blood ; Liver Failure, Acute/complications ; Liver Failure, Acute/therapy ; Microdialysis ; Pyruvic Acid/metabolism ; Sorption Detoxification ; Swine ; Time Factors
    Chemical Substances Glutamine (0RH81L854J) ; Lactic Acid (33X04XA5AT) ; Glutamic Acid (3KX376GY7L) ; Ammonia (7664-41-7) ; Pyruvic Acid (8558G7RUTR) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2013-06-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1471-230X
    ISSN (online) 1471-230X
    DOI 10.1186/1471-230X-13-98
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