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  1. Article ; Online: Transgenerational effects from single larval exposure to azadirachtin on life history and behavior traits of Drosophila melanogaster

    M. Ferdenache / R. Bezzar-Bendjazia / F. Marion-Poll / S. Kilani-Morakchi

    Scientific Reports, Vol 9, Iss 1, Pp 1-

    2019  Volume 12

    Abstract: Abstract Azadirachtin is one of the successful botanical pesticides in agricultural use with a broad-spectrum insecticide activity, but its possible transgenerational effects have not been under much scrutiny. The effects of sublethal doses of ... ...

    Abstract Abstract Azadirachtin is one of the successful botanical pesticides in agricultural use with a broad-spectrum insecticide activity, but its possible transgenerational effects have not been under much scrutiny. The effects of sublethal doses of azadirachtin on life-table traits and oviposition behaviour of a model organism in toxicological studies, D. melanogaster, were evaluated. The fecundity and oviposition preference of flies surviving to single azadirachtin-treated larvae of parental generation was adversely affected and resulted in the reduction of the number of eggs laid and increased aversion to this compound over two successive generations. In parental generation, early exposure to azadirachtin affects adult’s development by reducing the number of organisms, delay larval and pupal development; male biased sex ratio and induced morphological alterations. Moreover, adult’s survival of the two generations was significantly decreased as compared to the control. Therefore, Single preimaginal azadirachtin treatment can affect flies population dynamics via transgenerational reductions in survival and reproduction capacity as well as reinforcement of oviposition avoidance which can contribute as repellent strategies in integrated pest management programs. The transgenerational effects observed suggest a possible reduction both in application frequency and total amount of pesticide used, would help in reducing both control costs and possible ecotoxicological risks.
    Keywords Medicine ; R ; Science ; Q
    Subject code 590
    Language English
    Publishing date 2019-11-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Azadirachtin impact on mate choice, female sexual receptivity and male activity in Drosophila melanogaster (Diptera: Drosophilidae)

    Aribi, N / B. Denis / D. Joly / J.C. Sandoz / L. Kaiser / M.C. Oulhaci / S. Kilani-Morakchi

    Pesticide biochemistry and physiology. 2017 Nov., v. 143

    2017  

    Abstract: Azadirachtin, a neem compound (Azadirachta indica) with medical and anti-insect properties, is one the most successful botanical pesticides in agricultural use. However, its controversial impact on non-targeted species and its mechanism of action need to ...

    Abstract Azadirachtin, a neem compound (Azadirachta indica) with medical and anti-insect properties, is one the most successful botanical pesticides in agricultural use. However, its controversial impact on non-targeted species and its mechanism of action need to be clarified. In addition, Azadirachtin impact on pre- and post-mating traits remains largely undocumented. The current study examined the effects of Azadirachtin on Drosophila melanogaster as a non-target and model species. Azadirachtin was applied topically at its LD50 (0.63μg) on the day of adult emergence and its effect was evaluated on several traits of reproductive behavior: mate choice, male activity, female sexual receptivity, sperm storage and female sterility. In choice and no choice conditions, only male treatment reduced mating probability. Female treatment impaired mating probability only when males had the choice. Males' mating ability may have been impaired by an effect of the treatment on their mobility. Such an effect was observed in the actimeter, which revealed that treated males were less active than untreated ones, and this effect persisted over 8days. Azadirachtin treatment had, however, no effect on the nycthemeral rhythm of those males. Even when mating occurred, Azadirachtin treatment impaired post-mating responses especially when females or both sexes were treated: remating probability increases and female fertility (presence of larvae) decreases. No impairment was observed on the efficiency of mating, evaluated by the presence of sperm in the spermatheca or the ventral receptacle. Male treatment only had no significant effect on these post-mating responses. These findings provide clear evidence that Azadirachtin alters the reproductive behavior of both sexes in D. melanogaster via mating and post-mating processes.
    Keywords adults ; Azadirachta indica ; azadirachtin ; botanical pesticides ; Drosophila melanogaster ; female fertility ; females ; insecticidal properties ; larvae ; lethal dose 50 ; males ; mating behavior ; mechanism of action ; models ; probability ; reproductive behavior ; spermatheca ; spermatozoa ; ventral receptacle
    Language English
    Dates of publication 2017-11
    Size p. 95-101.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 184819-7
    ISSN 1095-9939 ; 0048-3583 ; 0048-3575
    ISSN (online) 1095-9939
    ISSN 0048-3583 ; 0048-3575
    DOI 10.1016/j.pestbp.2017.09.002
    Database NAL-Catalogue (AGRICOLA)

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