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Article ; Online: Quantitative evaluation of bronchoalveolar lavage for the treatment of Severe mycoplasma pneumoniae pneumonia in children-A new complementary index: Bronchial Insufflation Sign Score.

Gao, Jin / Wu, R / Zhang, Y J / Xu, X / Sa, R N / Li, X A / Liu, C Y

2024

Abstract: Objective: The aim of this study was to investigate the value of Broncoplasma Insufflation Sign in lung ultrasound signs in assessing the efficacy of bronchoalveolar lavage in Severe mycoplasma pneumoniae pneumonia in children.: Methods: Forty-seven ... ...

Abstract	Objective: The aim of this study was to investigate the value of Broncoplasma Insufflation Sign in lung ultrasound signs in assessing the efficacy of bronchoalveolar lavage in Severe mycoplasma pneumoniae pneumonia in children. Methods: Forty-seven children with Severe mycoplasma pneumoniae pneumonia were treated with medication and bronchial lavage. Laboratory and imaging results were collected, and lung ultrasonography was performed before bronchoalveolar lavage and 1, 3, and 7 days after lavage to record changes in Bronchial Insufflation Sign and changes in the extent of solid lung lesions. Factors affecting the effectiveness of bronchoalveolar lavage were analyzed using logistic regression and other factors. Results: Bronchial Insufflation Sign Score and the extent of lung solid lesions were the factors affecting the effectiveness of bronchoalveolar lavage treatment. The smaller the area of lung solid lesions and the higher the Bronchial Insufflation Sign Score, the more effective the results of bronchoalveolar lavage treatment were, and the difference was statistically significant, with a difference of p < 0.05. The Bronchial Insufflation Sign Score had the highest sensitivity and specificity for the prediction of the efficacy of bronchoalveolar lavage treatment in the first 7 days after the treatment. Conclusion: Bronchial Insufflation Sign Score combined with the extent of solid lung lesions can assess the efficacy of bronchoalveolar lavage in the treatment of Severe mycoplasma pneumoniae pneumonia in children; lung ultrasound is a timely and effective means of assessing the efficacy of bronchoalveolar lavage.
Language	English
Publishing date	2024-04-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	189393-2
ISSN	1097-0096 ; 0091-2751
ISSN (online)	1097-0096
ISSN	0091-2751
DOI	10.1002/jcu.23678
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Article: Using combined Lactobacillus and quorum quenching enzyme supplementation as an antibiotic alternative to improve broiler growth performance, anti-oxidative status, immune response, and gut microbiota

Sun, X.X. / Chen, D. D / Deng, S.Q. / Zhang, G.M. / Peng, X. / SA, R.N.

Poultry science. 2022 May 29,

2022

Abstract: To seek viable alternatives to antibiotics, we determined the combinatorial effects of Lactobacillus and a quorum quenching enzyme (QQE) on broiler growth performance, antioxidant capacity, immune responses, and cecal microbial populations. In total, 360 ...

Abstract	To seek viable alternatives to antibiotics, we determined the combinatorial effects of Lactobacillus and a quorum quenching enzyme (QQE) on broiler growth performance, antioxidant capacity, immune responses, and cecal microbial populations. In total, 360 one-day-old male broilers (Ross 308) were randomly allotted to three dietary treatments, with 12 replicate pens/treatment and 10 birds/replicate pen. Dietary treatments lasted 42 days and comprised: corn-soybean meal basal diet (control group, CON); control plus antibiotic growth promoter supplement group (AGP); and control plus Lactobacillus and QQE supplement group (LQ). Dietary LQ supplementation significantly increased final body weight (BW) and average daily gain (ADG) when compared with CON and AGP groups between 22 and 42 days and 1 to 42 days (P < 0.05). No significant differences were observed for serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels between treatments (P > 0.05). A higher concentration of total antioxidant capacity (T-AOC) was observed on day 42 in the LQ group (P = 0.06). Feeding LQ significantly increased serum immunoglobulins (IgA and IgG) levels when compared with other treatments (P < 0.05). A statistical trend was also observed for increased cecal butyrate levels (P = 0.06) in the LQ group. Bacterial α-diversity was unaffected by dietary treatments (P > 0.05). However, from principal component analysis (PCoA), the microbial community structure was significantly different between the LQ and AGP groups. Diet supplemented with LQ significantly (P < 0.05) decreased the relative abundance of Synergistota and Proteobacteria and significantly (P < 0.05) increased the proportion of Ruminococcaceae and Faecalibacterium. Thus, supplemental LQ improved growth performance, immune status, and modulated intestinal microbial communities in broilers. We provide a new perceptive on antibiotic substitutes in the poultry industry.
Keywords	Lactobacillus ; Proteobacteria ; Ruminococcaceae ; antibiotics ; antioxidant activity ; average daily gain ; blood serum ; body weight ; butyrates ; community structure ; diet ; glutathione peroxidase ; growth performance ; growth promotion ; immune response ; intestinal microorganisms ; intestines ; males ; malondialdehyde ; microbial communities ; poultry industry ; principal component analysis ; superoxide dismutase
Language	English
Dates of publication	2022-0529
Publishing place	Elsevier Inc.
Document type	Article
Note	Pre-press version
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2022.101997
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Article ; Online: Using combined Lactobacillus and quorum quenching enzyme supplementation as an antibiotic alternative to improve broiler growth performance, antioxidative status, immune response, and gut microbiota.

Sun, X X / Chen, D D / Deng, S Q / Zhang, G M / Peng, X / Sa, R N

Poultry science

2022 Volume 101, Issue 9, Page(s) 101997

Abstract	To seek viable alternatives to antibiotics, we determined the combinatorial effects of Lactobacillus and a quorum quenching enzyme (QQE) on broiler growth performance, antioxidant capacity, immune responses, and cecal microbial populations. In total, 360 one-day-old male broilers (Ross 308) were randomly allotted to 3 dietary treatments, with 12 replicate pens/treatment and 10 birds/replicate pen. Dietary treatments lasted 42 d and comprised: corn-soybean meal basal diet (control group, CON); control plus antibiotic growth promoter supplement group (AGP); and control plus Lactobacillus and QQE supplement group (LQ). Dietary LQ supplementation significantly increased final body weight (BW) and average daily gain (ADG) when compared with CON and AGP groups between 22 and 42 d and 1 to 42 d (P < 0.05). No significant differences were observed for serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels between treatments (P > 0.05). A higher concentration of total antioxidant capacity (T-AOC) was observed on d 42 in the LQ group (P = 0.06). Feeding LQ significantly increased serum immunoglobulins (IgA and IgG) levels when compared with other treatments (P < 0.05). A statistical trend was also observed for increased cecal butyrate levels (P = 0.06) in the LQ group. Bacterial α-diversity was unaffected by dietary treatments (P > 0.05). However, from principal component analysis (PCoA), the microbial community structure was different between the LQ and AGP groups. Diet supplemented with LQ significantly (P < 0.05) decreased the relative abundance of Synergistota and Proteobacteria and significantly (P < 0.05) increased the proportion of Ruminococcaceae and Faecalibacterium. Thus, supplemental LQ improved growth performance, immune status, and modulated intestinal microbial communities in broilers. We provide a new perceptive on antibiotic substitutes in the poultry industry.
MeSH term(s)	Animal Feed/analysis ; Animals ; Anti-Bacterial Agents/pharmacology ; Antioxidants/pharmacology ; Chickens/physiology ; Diet/veterinary ; Dietary Supplements/analysis ; Gastrointestinal Microbiome ; Immunity ; Lactobacillus ; Male ; Quorum Sensing
Chemical Substances	Anti-Bacterial Agents ; Antioxidants
Language	English
Publishing date	2022-06-10
Publishing country	England
Document type	Journal Article ; Randomized Controlled Trial, Veterinary
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2022.101997
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Article: [Trends of smoking and passive smoking in residents in Shaanxi province: an analysis on results of four cross-sectional surveys, 2007-2015].

Wang, W H / Liu, C Y / Sa, R N / Qiu, L

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi

2019 Volume 40, Issue 11, Page(s) 1420–1425

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Adolescent ; Adult ; Asian Continental Ancestry Group/statistics & numerical data ; China/epidemiology ; Cross-Sectional Studies ; Female ; Humans ; Male ; Population Surveillance/methods ; Smoking/epidemiology ; Smoking/ethnology ; Smoking/trends ; Smoking Cessation/psychology ; Smoking Cessation/statistics & numerical data ; Surveys and Questionnaires ; Tobacco Smoke Pollution/statistics & numerical data
Chemical Substances	Tobacco Smoke Pollution
Language	Chinese
Publishing date	2019-12-14
Publishing country	China
Document type	Journal Article
ZDB-ID	645026-x
ISSN	0254-6450
ISSN	0254-6450
DOI	10.3760/cma.j.issn.0254-6450.2019.11.015
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Article ; Online: Comparison of amino acid digestibility and its additivity determined with slaughter or cecectomy method for yellow-feather chicken

Liu, H. S. / Zhao, F. / Chen, J. / Zou, Y. / Yu, Y. / Wang, Y. M. / Liu, S. B. / Tan, H. Z. / Sa, R. N. / Xie, J. J.

Poultry Science. 2022 Dec., v. 101, no. 12, p. 102196

2022 , Page(s) 102196

Abstract: The objective of this experiment was to compare the slaughter and cecectomy methods to determine amino acid (AA) digestibility of corn and soybean meal and their additivity in a corn-soybean meal diet. A completely randomized design was adopted to ... ...

Abstract	The objective of this experiment was to compare the slaughter and cecectomy methods to determine amino acid (AA) digestibility of corn and soybean meal and their additivity in a corn-soybean meal diet. A completely randomized design was adopted to determine endogenous AA losses (EAAL) and AA digestibility in each of corn, soybean meal, and a corn-soybean meal diet using either slaughter or cecectomy methods. Each treatment contained 6 replicates with 3 chickens per replicate. The endogenous loss (EL) of histidine and glycine was lower and the EL of methionine and phenylalanine was greater when determined by slaughter vs. cecectomy (P < 0.05). The EL of arginine, isoleucine, leucine, lysine, methionine, phenylalanine, valine, alanine, aspartic acid, glutamic acid, and serine determined by slaughter were 1.2 to 3.2 times of those from cecectomy. The standard error (SE) of EL of 14 AA (excluding histidine and glycine) obtained by slaughter method was 2.1 to 9.6 times of those by cecectomy method. The apparent and standardized digestibility was not affected by methods for most AA except apparent digestibility of methionine, phenylalanine and glycine, and standardized digestibility of glycine in corn. The apparent and standardized digestibility of most AA except apparent digestibility of glycine and standardized digestibility of lysine, cysteine and glycine were less for slaughter versus cecectomy methods in soybean meal (P < 0.05). Using slaughter method resulted in reduced apparent digestibility of 15 AA (except glycine) and reduced standardized digestibility of 7 AA (arginine, isoleucine, leucine, valine, aspartic acid, glutamic acid, and proline) relative to cecectomy method (P < 0.05), but the standardized digestibility of glycine was greater when determined by slaughter vs. cecectomy methods in corn-soybean meal diet (P < 0.05). The mean value of SE of 16 AA digestibility in slaughter method was 2.9 times of that by cecectomy method. The apparent digestibility of 2 and 9 of 16 AA and the standardized digestibility of 15 and 7 of 16 AA were additive when using slaughter and cecectomy determinations, respectively. In conclusion, compared to the slaughter method, cecectomy method had less SE and EAAL but greater apparent digestibility of methionine and phenylalanine in corn, and the apparent digestibility of 15 AA (except glycine) in soybean meal and corn-soybean meal diet. Additivity in apparent and standardized AA digestibility was more inconsistent when determined with slaughter vs. cecectomy methods. These findings suggest that the cecectomy method is more suitable than the slaughter method to determine the digestibility of AA.
Keywords	alanine ; arginine ; aspartic acid ; chickens ; corn ; cysteine ; diet ; digestibility ; digestible amino acids ; glutamic acid ; histidine ; isoleucine ; leucine ; lysine ; methionine ; phenylalanine ; proline ; serine ; slaughter ; soybean meal ; valine ; additivity ; amino acid digestibility ; cecectomy method ; slaughter method
Language	English
Dates of publication	2022-12
Size	p. 102196
Publishing place	Elsevier Inc.
Document type	Article ; Online
Note	Pre-press version ; Use and reproduction
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2022.102196
Database	NAL-Catalogue (AGRICOLA)

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Article: Bacillus amyloliquefaciens spray improves the growth performance, immune status, and respiratory mucosal barrier in broiler chickens

Luan, S J / Sa, R N / Sun, Y B / Wang, Y / Zhang, H F

Poultry science. 2019 Mar. 01, v. 98, no. 3

2019

Abstract: Studies have shown that supplementing probiotics in a nasal spray can provide prophylaxis against upper respiratory tract infections. Previously, we found that spraying with Bacillus reduced the concentration of atmospheric ammonia and airborne ... ...

Abstract	Studies have shown that supplementing probiotics in a nasal spray can provide prophylaxis against upper respiratory tract infections. Previously, we found that spraying with Bacillus reduced the concentration of atmospheric ammonia and airborne microbiological aerosols in broiler houses. In the present study, we evaluated the effects of spraying with Bacillus amyloliquefaciens on the respiratory mucosal barrier in broilers. Two Trials were conducted simultaneously in four environmentally controlled chambers. In each trail, 320 1-d-old male broilers were randomly assigned to control (CON) and B. amyloliquefaciens (BAQ) group. Eight cages containing 20 birds per cage were placed in two chambers. The BAQ group chambers were sprayed with B. amyloliquefaciens at 4 × 109 CFU/m2 each day for 42 d. Spraying with B. amyloliquefaciens increased average daily gain and average daily feed intake in broilers (P < 0.01), but had no effects on FCR (P > 0.05). The relative weights of the bursa (21 d) and spleen (42 d) were higher in the BAQ group (P < 0.05). The serum IgG, IgM (21 d), and IgA, Ig M (42 d) levels were improved in BAQ group (P < 0.05). The sIgA levels in tracheal lavage fluid were significantly higher in the BAQ group than the CON group (P < 0.05). Furthermore, treatment with BAQ downregulated IL-6 and IFN-γ (P < 0.05), but upregulated IL-2, occludin, and mucin-2 (P < 0.05). In addition, the number of acidic goblet cells was significantly higher in the tracheas of treated broilers than untreated broilers (P < 0.05). In conclusion, spraying with B. amyloliquefaciens may help to enhance the growth performance and improve the respiratory mucosal barrier in broiler chickens.
Keywords	aerosols ; ammonia ; average daily gain ; Bacillus amyloliquefaciens ; blood serum ; broiler chickens ; cages ; disease control ; feed intake ; goblet cells ; growth performance ; immunoglobulin A ; immunoglobulin G ; immunoglobulin M ; interferon-gamma ; interleukin-2 ; interleukin-6 ; males ; nose ; occludins ; poultry housing ; probiotics ; respiratory tract diseases ; spleen ; spraying
Language	English
Dates of publication	2019-0301
Size	p. 1403-1409.
Publishing place	Poultry Science Association, Inc.
Document type	Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.3382/ps/pey478
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Comparison of amino acid digestibility and its additivity determined with slaughter or cecectomy method for yellow-feather chicken.

Liu, H S / Zhao, F / Chen, J / Zou, Y / Yu, Y / Wang, Y M / Liu, S B / Tan, H Z / Sa, R N / Xie, J J

Poultry science

2022 Volume 101, Issue 12, Page(s) 102196

Abstract	The objective of this experiment was to compare the slaughter and cecectomy methods to determine amino acid (AA) digestibility of corn and soybean meal and their additivity in a corn-soybean meal diet. A completely randomized design was adopted to determine endogenous AA losses (EAAL) and AA digestibility in each of corn, soybean meal, and a corn-soybean meal diet using either slaughter or cecectomy methods. Each treatment contained 6 replicates with 3 chickens per replicate. The endogenous loss (EL) of histidine and glycine was lower and the EL of methionine and phenylalanine was greater when determined by slaughter vs. cecectomy (P < 0.05). The EL of arginine, isoleucine, leucine, lysine, methionine, phenylalanine, valine, alanine, aspartic acid, glutamic acid, and serine determined by slaughter were 1.2 to 3.2 times of those from cecectomy. The standard error (SE) of EL of 14 AA (excluding histidine and glycine) obtained by slaughter method was 2.1 to 9.6 times of those by cecectomy method. The apparent and standardized digestibility was not affected by methods for most AA except apparent digestibility of methionine, phenylalanine and glycine, and standardized digestibility of glycine in corn. The apparent and standardized digestibility of most AA except apparent digestibility of glycine and standardized digestibility of lysine, cysteine and glycine were less for slaughter versus cecectomy methods in soybean meal (P < 0.05). Using slaughter method resulted in reduced apparent digestibility of 15 AA (except glycine) and reduced standardized digestibility of 7 AA (arginine, isoleucine, leucine, valine, aspartic acid, glutamic acid, and proline) relative to cecectomy method (P < 0.05), but the standardized digestibility of glycine was greater when determined by slaughter vs. cecectomy methods in corn-soybean meal diet (P < 0.05). The mean value of SE of 16 AA digestibility in slaughter method was 2.9 times of that by cecectomy method. The apparent digestibility of 2 and 9 of 16 AA and the standardized digestibility of 15 and 7 of 16 AA were additive when using slaughter and cecectomy determinations, respectively. In conclusion, compared to the slaughter method, cecectomy method had less SE and EAAL but greater apparent digestibility of methionine and phenylalanine in corn, and the apparent digestibility of 15 AA (except glycine) in soybean meal and corn-soybean meal diet. Additivity in apparent and standardized AA digestibility was more inconsistent when determined with slaughter vs. cecectomy methods. These findings suggest that the cecectomy method is more suitable than the slaughter method to determine the digestibility of AA.
MeSH term(s)	Animals ; Chickens/metabolism ; Amino Acids/metabolism ; Animal Nutritional Physiological Phenomena ; Digestion ; Animal Feed/analysis ; Feathers ; Leucine/metabolism ; Isoleucine/metabolism ; Lysine/metabolism ; Glutamic Acid ; Aspartic Acid/metabolism ; Histidine/metabolism ; Diet/veterinary ; Zea mays/chemistry ; Glycine max/chemistry ; Glycine/metabolism ; Methionine/metabolism ; Phenylalanine/metabolism ; Valine/metabolism ; Arginine/metabolism ; Ileum/metabolism
Chemical Substances	Amino Acids ; Leucine (GMW67QNF9C) ; Isoleucine (04Y7590D77) ; Lysine (K3Z4F929H6) ; Glutamic Acid (3KX376GY7L) ; Aspartic Acid (30KYC7MIAI) ; Histidine (4QD397987E) ; Glycine (TE7660XO1C) ; Methionine (AE28F7PNPL) ; Phenylalanine (47E5O17Y3R) ; Valine (HG18B9YRS7) ; Arginine (94ZLA3W45F)
Language	English
Publishing date	2022-09-21
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2022.102196
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Article: [Analysis of trends on smoking prevalence and its risk factors in Shaanxi province 2007-2015].

Wang, W H / Qiu, L / Sa, R N / Hu, Z P / Liu, R / Wu, M / Liu, F / Zhang, T H

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi

2020 Volume 41, Issue 3, Page(s) 395–399

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Adolescent ; Adult ; China/epidemiology ; Female ; Humans ; Male ; Middle Aged ; Prevalence ; Risk Factors ; Smoking/epidemiology ; Socioeconomic Factors ; Young Adult
Language	Chinese
Publishing date	2020-04-12
Publishing country	China
Document type	Journal Article
ZDB-ID	645026-x
ISSN	0254-6450
ISSN	0254-6450
DOI	10.3760/cma.j.issn.0254-6450.2020.03.022
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Article: [Analysis on chronic diseases and risk factors of adult residents in Shaanxi province].

Wang, W H / Sa, R N / Qiu, L / Hu, Z P / Liu, R / Wu, M / Liu, F / Dang, S N

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi

2020 Volume 40, Issue 12, Page(s) 1585–1589

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Adolescent ; Adult ; China ; Chronic Disease/epidemiology ; Cross-Sectional Studies ; Female ; Humans ; Hypertension/epidemiology ; Male ; Obesity/epidemiology ; Overweight/epidemiology ; Prevalence ; Risk Factors ; Rural Population ; Sedentary Behavior
Language	Chinese
Publishing date	2020-02-15
Publishing country	China
Document type	Journal Article
ZDB-ID	645026-x
ISSN	0254-6450
ISSN	0254-6450
DOI	10.3760/cma.j.issn.0254-6450.2019.12.015
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Article ; Online: Bacillus amyloliquefaciens spray improves the growth performance, immune status, and respiratory mucosal barrier in broiler chickens.

Luan, S J / Sun, Y B / Wang, Y / Sa, R N / Zhang, H F

Poultry science

2018 Volume 98, Issue 3, Page(s) 1403–1409

Abstract	Studies have shown that supplementing probiotics in a nasal spray can provide prophylaxis against upper respiratory tract infections. Previously, we found that spraying with Bacillus reduced the concentration of atmospheric ammonia and airborne microbiological aerosols in broiler houses. In the present study, we evaluated the effects of spraying with Bacillus amyloliquefaciens on the respiratory mucosal barrier in broilers. Two trails were conducted simultaneously in four environmentally controlled chambers. In each trail, 320 1-d-old male broilers were randomly assigned to control (CON) and B. amyloliquefaciens (BAQ) group. Eight cages containing 20 birds per cage were placed in two chambers. The BAQ group chambers were sprayed with B. amyloliquefaciens at 4 × 109 CFU/m2 each day for 42 d. Spraying with B. amyloliquefaciens increased average daily gain and average daily feed intake in broilers (P < 0.01), but had no effects on FCR (P > 0.05). The relative weights of the bursa (21 d) and spleen (42 d) were higher in the BAQ group (P < 0.05). The serum IgG, IgM (21 d), and IgA, Ig M (42 d) levels were improved in BAQ group (P < 0.05). The sIgA levels in tracheal lavage fluid were significantly higher in the BAQ group than the CON group (P < 0.05). Furthermore, treatment with BAQ downregulated IL-6 and IFN-γ (P < 0.05), but upregulated IL-2, occludin, and mucin-2 (P < 0.05). In addition, the number of acidic goblet cells was significantly higher in the tracheas of treated broilers than untreated broilers (P < 0.05). In conclusion, spraying with B. amyloliquefaciens may help to enhance the growth performance and improve the respiratory mucosal barrier in broiler chickens.
MeSH term(s)	Animal Husbandry/methods ; Animals ; Bacillus amyloliquefaciens ; Chickens/growth & development ; Chickens/immunology ; Cytokines ; Eating ; Immunoglobulins/blood ; Male ; Organ Size ; Probiotics/administration & dosage ; Respiratory Mucosa/physiology
Chemical Substances	Cytokines ; Immunoglobulins
Language	English
Publishing date	2018-09-23
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.3382/ps/pey478
Database	MEDical Literature Analysis and Retrieval System OnLINE

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