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  1. Article ; Online: Plasmonic Approaches for the Detection of SARS-CoV-2 Viral Particles.

    Szunerits, Sabine / Saada, Hiba / Pagneux, Quentin / Boukherroub, Rabah

    Biosensors

    2022  Volume 12, Issue 7

    Abstract: The ongoing highly contagious Coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), underlines the fundamental position of diagnostic testing in outbreak control by allowing a distinction of ...

    Abstract The ongoing highly contagious Coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), underlines the fundamental position of diagnostic testing in outbreak control by allowing a distinction of the infected from the non-infected people. Diagnosis of COVID-19 remains largely based on reverse transcription PCR (RT-PCR), identifying the genetic material of the virus. Molecular testing approaches have been largely proposed in addition to infectivity testing of patients via sensing the presence of viral particles of SARS-CoV-2 specific structural proteins, such as the spike glycoproteins (S1, S2) and the nucleocapsid (N) protein. While the S1 protein remains the main target for neutralizing antibody treatment upon infection and the focus of vaccine and therapeutic design, it has also become a major target for the development of point-of care testing (POCT) devices. This review will focus on the possibility of surface plasmon resonance (SPR)-based sensing platforms to convert the receptor-binding event of SARS-CoV-2 viral particles into measurable signals. The state-of-the-art SPR-based SARS-CoV-2 sensing devices will be provided, and highlights about the applicability of plasmonic sensors as POCT for virus particle as well as viral protein sensing will be discussed.
    MeSH term(s) Antibodies, Viral ; COVID-19/diagnosis ; Humans ; Pandemics ; SARS-CoV-2 ; Virion
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2022-07-21
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios12070548
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Luminescence Amplification at BiVO

    Yu, Jing / Saada, Hiba / Abdallah, Rawa / Loget, Gabriel / Sojic, Neso

    Angewandte Chemie (International ed. in English)

    2020  Volume 59, Issue 35, Page(s) 15157–15160

    Abstract: Photoinduced electrochemiluminescence (PECL) combines semiconductor (SC) photoelectrochemistry with electrochemiluminescence (ECL). In PECL, the incident light is converted into a different wavelength by an electrochemical reaction at a SC photoelectrode ...

    Abstract Photoinduced electrochemiluminescence (PECL) combines semiconductor (SC) photoelectrochemistry with electrochemiluminescence (ECL). In PECL, the incident light is converted into a different wavelength by an electrochemical reaction at a SC photoelectrode and allows triggering of ECL at low potentials. This concept has been employed to design up-conversion systems. However, PECL strongly suffers from the photoelectrochemical instability of these low band gap SCs. Reported here for the first time is an original light-conversion strategy based on PECL of a luminol derivative (L-012) at BiVO
    Language English
    Publishing date 2020-06-09
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.202004634
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: A mask-based diagnostic platform for point-of-care screening of Covid-19

    Daniels, John / Wadekar, Shekhar / DeCubellis, Ken / Jackson, George W. / Chiu, Alexander S. / Pagneux, Quentin / Saada, Hiba / Engelmann, Ilka / Ogiez, Judith / Loze-Warot, Delphine / Boukherroub, Rabah / Szunerits, Sabine

    Biosensors & bioelectronics. 2021 Nov. 15, v. 192

    2021  

    Abstract: Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self- ... ...

    Abstract Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self-testing approaches. In this study, we introduce a different concept based on exhaled breath condensate (EBC), readily collected by a mask-based sampling device, and detection with an electrochemical biosensor with a modular architecture that enables fast and specific detection and quantification of COVID-19. The face mask forms an exhaled breath vapor containment volume to hold the exhaled breath vapor in proximity to the EBC collector to enable a condensate-forming surface, cooled by a thermal mass, to coalesce the exhaled breath into a 200–500 μL fluid sample in 2 min. EBC RT-PCR for SARS-CoV-2 genes (E, ORF1ab) on samples collected from 7 SARS-CoV-2 positive and 7 SARS-CoV-2 negative patients were performed. The presence of SARS-CoV-2 could be detected in 5 out of 7 SARS-CoV-2 positive patients. Furthermore, the EBC samples were screened on an electrochemical aptamer biosensor, which detects SARS-CoV-2 viral particles down to 10 pfu mL⁻¹ in cultured SARS-CoV-2 suspensions. Using a “turn off” assay via ferrocenemethanol redox mediator, results about the infectivity state of the patient are obtained in 10 min.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; biosensors ; condensates ; diagnostic techniques ; electrochemistry ; face masks ; oligonucleotides ; pathogenicity ; patients ; point-of-care systems ; reverse transcriptase polymerase chain reaction ; vapors
    Language English
    Dates of publication 2021-1115
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2021.113486
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: SARS-CoV-2 detection using a nanobody-functionalized voltammetric device.

    Pagneux, Quentin / Roussel, Alain / Saada, Hiba / Cambillau, Christian / Amigues, Béatrice / Delauzun, Vincent / Engelmann, Ilka / Alidjinou, Enagnon Kazali / Ogiez, Judith / Rolland, Anne Sophie / Faure, Emmanuel / Poissy, Julien / Duhamel, Alain / Boukherroub, Rabah / Devos, David / Szunerits, Sabine

    Communications medicine

    2022  Volume 2, Page(s) 56

    Abstract: Background: An ongoing need during the COVID-19 pandemic has been the requirement for accurate and efficient point-of-care testing platforms to distinguish infected from non-infected people, and to differentiate SARS-CoV-2 infections from other viruses. ...

    Abstract Background: An ongoing need during the COVID-19 pandemic has been the requirement for accurate and efficient point-of-care testing platforms to distinguish infected from non-infected people, and to differentiate SARS-CoV-2 infections from other viruses. Electrochemical platforms can detect the virus via its envelope spike protein by recording changes in voltammetric signals between samples. However, this remains challenging due to the limited sensitivity of these sensing platforms.
    Methods: Here, we report on a nanobody-functionalized electrochemical platform for the rapid detection of whole SARS-CoV-2 viral particles in complex media such as saliva and nasopharyngeal swab samples. The sensor relies on the functionalization of gold electrode surface with highly-oriented Llama nanobodies specific to the spike protein receptor binding domain (RBD). The device provides results in 10 min of exposure to 200 µL of unprocessed samples with high specificity to SARS-CoV-2 viral particles in human saliva and nasopharyngeal swab samples.
    Results: The developed sensor could discriminate between different human coronavirus strains and other respiratory viruses, with 90% positive and 90% negative percentage agreement on 80 clinical samples, as compared to RT-qPCR.
    Conclusions: We believe this diagnostic concept, also validated for RBD mutants and successfully tested on Delta variant samples, to be a powerful tool to detect patients' infection status, easily extendable to other viruses and capable of overcoming sensing-related mutation effects.
    Language English
    Publishing date 2022-05-23
    Publishing country England
    Document type Journal Article
    ISSN 2730-664X
    ISSN (online) 2730-664X
    DOI 10.1038/s43856-022-00113-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A mask-based diagnostic platform for point-of-care screening of Covid-19.

    Daniels, John / Wadekar, Shekhar / DeCubellis, Ken / Jackson, George W / Chiu, Alexander S / Pagneux, Quentin / Saada, Hiba / Engelmann, Ilka / Ogiez, Judith / Loze-Warot, Delphine / Boukherroub, Rabah / Szunerits, Sabine

    Biosensors & bioelectronics

    2021  Volume 192, Page(s) 113486

    Abstract: Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self- ... ...

    Abstract Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self-testing approaches. In this study, we introduce a different concept based on exhaled breath condensate (EBC), readily collected by a mask-based sampling device, and detection with an electrochemical biosensor with a modular architecture that enables fast and specific detection and quantification of COVID-19. The face mask forms an exhaled breath vapor containment volume to hold the exhaled breath vapor in proximity to the EBC collector to enable a condensate-forming surface, cooled by a thermal mass, to coalesce the exhaled breath into a 200-500 μL fluid sample in 2 min. EBC RT-PCR for SARS-CoV-2 genes (E, ORF1ab) on samples collected from 7 SARS-CoV-2 positive and 7 SARS-CoV-2 negative patients were performed. The presence of SARS-CoV-2 could be detected in 5 out of 7 SARS-CoV-2 positive patients. Furthermore, the EBC samples were screened on an electrochemical aptamer biosensor, which detects SARS-CoV-2 viral particles down to 10 pfu mL
    Language English
    Publishing date 2021-07-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2021.113486
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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