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Article ; Online: Plant viruses traveling without passport.

Sáez, Cristina / Pagán, Israel

2024 Volume 22, Issue 5, Page(s) e3002626

Abstract: All plant viruses were thought to encode in its genome a movement protein that acts as a "passport," allowing active movement within the host. A new study in PLOS Biology characterizes the first plant virus that can colonize its host without encoding ... ...

Abstract	All plant viruses were thought to encode in its genome a movement protein that acts as a "passport," allowing active movement within the host. A new study in PLOS Biology characterizes the first plant virus that can colonize its host without encoding this protein.
MeSH term(s)	Plant Viruses/physiology ; Plant Viruses/genetics ; Plant Viruses/pathogenicity ; Plant Diseases/virology ; Plants/virology ; Plant Viral Movement Proteins/metabolism ; Plant Viral Movement Proteins/genetics ; Genome, Viral ; Host-Pathogen Interactions
Chemical Substances	Plant Viral Movement Proteins
Language	English
Publishing date	2024-05-10
Publishing country	United States
Document type	Journal Article ; Comment
ZDB-ID	2126776-5
ISSN	1545-7885 ; 1544-9173
ISSN (online)	1545-7885
ISSN	1544-9173
DOI	10.1371/journal.pbio.3002626
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Article: Capillary blood stability and analytical accuracy of 12 analytes stored in Microtainers®.

Fontaine, Elizabeth / Saez, Cristian

Practical laboratory medicine

2023 Volume 36, Page(s) e00325

Abstract: Objectives: The aim of this study was to determine feasibility of collecting capillary blood by traditional fingerstick and next day analysis after transport in Microtainers® at ambient temperature with no plasma separation. This study is pursuing an ... ...

Abstract	Objectives: The aim of this study was to determine feasibility of collecting capillary blood by traditional fingerstick and next day analysis after transport in Microtainers® at ambient temperature with no plasma separation. This study is pursuing an acceptable alternative to venipuncture for measuring 12 analytes important for health risk assessment. Design: and Methods: Performance standards of a 12-assay chemistry panel were assessed using a set of paralleled serum and capillary microsamples. The panel included Hemoglobin A1c (HbA1c), Total Cholesterol, Triglycerides, HDL-C, Creatinine, Urea Nitrogen (BUN), Uric Acid, alkaline phosphatase (ALP), ALT (GPT), AST (GOT), gamma-glutamyltransferase (GGT), and total protein. Correlation studies were performed using 31 simultaneous venous and capillary blood collections. Analytical bias, correlation, and medical decision points were calculated to determine equivalency of sample type and the impact of transport conditions. Clinical sensitivity, specificity, and predictive values were evaluated at calculated medical decision points for their usability in health screening initiatives. Results: Laboratory test results using capillary blood samples stored in Microtainers® under conditions of delayed centrifugation, and mail transport at ambient temperature, showed an acceptable agreement with results obtained using their paired serum samples analyzed using standard methods, except AST. Conclusions: Capillary blood samples can be self-collected at remote locations using Microtainers® and transported at ambient temperature for 24 h for successful performance of several medical tests important in large-scale health screenings programs.
Language	English
Publishing date	2023-07-05
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2834973-8
ISSN	2352-5517
ISSN	2352-5517
DOI	10.1016/j.plabm.2023.e00325
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Article ; Online: When plants are Trojan horses for viruses.

Sáez, Cristina / Pagán, Israel

The New phytologist

2022 Volume 237, Issue 4, Page(s) 1071–1073

MeSH term(s)	Plants ; Arabidopsis ; Viruses ; Plant Diseases ; Plant Viruses
Language	English
Publishing date	2022-12-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	208885-x
ISSN	1469-8137 ; 0028-646X
ISSN (online)	1469-8137
ISSN	0028-646X
DOI	10.1111/nph.18571
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Article: Analysis of SARS-CoV-2 antibodies from dried blood spot samples with the Roche Elecsys Immunochemistry method.

Fontaine, Elizabeth / Saez, Cristian

Practical laboratory medicine

2021 Volume 25, Page(s) e00234

Abstract: Objectives: The aim of this study was to evaluate the application of the Roche Elecsys anti-SARS-CoV-2 assay to capillary dried blood samples for high throughput analyses on Roche COBAS 6000 systems.: Design and methods: The performance of the of the ...

Abstract	Objectives: The aim of this study was to evaluate the application of the Roche Elecsys anti-SARS-CoV-2 assay to capillary dried blood samples for high throughput analyses on Roche COBAS 6000 systems. Design and methods: The performance of the of the Elecsys anti-SARS-CoV-2 assay was assessed using three sets of dried blood spot samples. Method correlation was performed using spiked blood samples. Sensitivity and specificity were calculated using paired donor samples. An additional cohort of 50 individuals, including COVID-19 convalescent cases, was used for the evaluation of at-home collection for mail transport, and stability studies. Results: The Elecsys anti-SARS-CoV-2 assay using dried blood spot samples showed an excellent agreement of 98.9% with results obtained using their paired serum samples, and 86.7% accuracy with dried blood spots collected after 9 days from diagnostic (PCR) tests. Conclusions: Capillary dried blood spot samples can be confidently used on Roche COBAS automated analyzers to monitor the epidemiology of COVID-19, and are suitable for use in large-scale screening programs.
Language	English
Publishing date	2021-04-28
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2834973-8
ISSN	2352-5517
ISSN	2352-5517
DOI	10.1016/j.plabm.2021.e00234
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Article ; Online: Process-Based and Probabilistic Quantification of Co and Ni Mobilization Risks Induced by Managed Aquifer Recharge.

Vergara-Sáez, Claudio / Prommer, Henning / Siade, Adam J / Sun, Jing / Higginson, Simon

Environmental science & technology

2024 Volume 58, Issue 17, Page(s) 7567–7576

Abstract: Managed aquifer recharge (MAR) is an increasingly used water management technique that enhances water availability while commonly generating water quality benefits. However, MAR activities may also trigger adverse geochemical reactions, especially during ...

Abstract	Managed aquifer recharge (MAR) is an increasingly used water management technique that enhances water availability while commonly generating water quality benefits. However, MAR activities may also trigger adverse geochemical reactions, especially during the injection of oxidant-enriched waters into reducing aquifers. Where this occurs, the environmental risks and the viability of mitigating them must be well understood. Here, we develop a rigorous approach for assessing and managing the risks from MAR-induced metal mobilization. First, we develop a process-based reactive transport model to identify and quantify the main hydrogeochemical drivers that control the release of metals and their mobility. We then apply a probabilistic framework to interrogate the inherent uncertainty associated with adjustable model parameters and consider this uncertainty (i) in long-term predictions of groundwater quality changes and (ii) in scenarios that investigate the effectiveness of modifications in the water treatment process to mitigate metal release and mobility. The results suggested that Co, Ni, Zn, and Mn were comobilized during pyrite oxidation and that metal mobility was controlled (i) by the sediment pH buffering capacity and (ii) by the sorption capacity of the native aquifer sediments. Both tested mitigation strategies were shown to be effective at reducing the risk of elevated metal concentrations.
MeSH term(s)	Groundwater/chemistry ; Water Pollutants, Chemical ; Nickel ; Cobalt
Chemical Substances	Water Pollutants, Chemical ; Nickel (7OV03QG267) ; Cobalt (3G0H8C9362)
Language	English
Publishing date	2024-04-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.3c10583
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Article: Treatment of Organics in Wastewater Using Electrogenerated Gaseous Oxidants.

da Silva, Leticia Mirella / Mena, Ismael F / Saez, Cristina / Motheo, Artur J / Rodrigo, Manuel A

Industrial & engineering chemistry research

2024 Volume 63, Issue 15, Page(s) 6512–6520

Abstract: This work focuses on the comparison of the performance of direct electrochemical oxidation with indirect electrolysis mediated by gaseous oxidants in the treatment of diluted wastewater. To do this, energy consumptions of the electrolysis using mixed ... ...

Abstract	This work focuses on the comparison of the performance of direct electrochemical oxidation with indirect electrolysis mediated by gaseous oxidants in the treatment of diluted wastewater. To do this, energy consumptions of the electrolysis using mixed metal oxide (MMO) electrodes are compared with those required for the production and use of chlorine dioxide in the degradation of methomyl contained in aqueous solutions. Results demonstrate the feasibility of the mediated oxidation process and that this process is competitive with direct oxidation. The oxidants are produced under optimized conditions using the same anodic material applied for the direct degradation of organics, thus avoiding efficiency losses associated with mass transfer limitations in the degradation of dilute organic solutions. Thus, using the ClO
Language	English
Publishing date	2024-04-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	1484436-9
ISSN	1520-5045 ; 0888-5885
ISSN (online)	1520-5045
ISSN	0888-5885
DOI	10.1021/acs.iecr.3c03265
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Article ; Online: Quantification of Plant Virus Seed Transmission Rate in Arabidopsis thaliana.

Gil-Valle, Miriam / Sáez, Cristina / Montes, Nuria / Pagán, Israel

Methods in molecular biology (Clifton, N.J.)

2023 Volume 2724, Page(s) 181–192

Abstract: More than 25% of all known plant viruses are transmitted through seeds, which makes this mode of dispersal of great importance for plant virus epidemics. Virus detection in seed stocks remains the most frequent approach for seed health testing, but ... ...

Abstract	More than 25% of all known plant viruses are transmitted through seeds, which makes this mode of dispersal of great importance for plant virus epidemics. Virus detection in seed stocks remains the most frequent approach for seed health testing, but current methods are not always standardized and/or do not allow analyzing large numbers of seeds. Here, we describe a high-throughput method to quantify plant virus seed transmission rate based on classical grow-out tests, which can be applied to widely different viruses and host species.
MeSH term(s)	Arabidopsis ; Plant Viruses/genetics ; Seeds ; Host Specificity
Language	English
Publishing date	2023-11-21
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3485-1_13
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Article ; Online: Contaminants of emerging concern in the urban aquifers of Barcelona: Do they hamper the use of groundwater?

Nikolenko, Olha / Pujades, Estanislao / Teixidó, Marc / Sáez, Carmen / Jurado, Anna

Chemosphere

2023 Volume 341, Page(s) 140023

Abstract: Urban aquifers are an alternative to obtain freshwater, but they are frequently polluted by contaminants of emerging concern (CECs). Therefore, there is a need to ascertain whether CECs are a water management challenge as they might limit the use of ... ...

Abstract	Urban aquifers are an alternative to obtain freshwater, but they are frequently polluted by contaminants of emerging concern (CECs). Therefore, there is a need to ascertain whether CECs are a water management challenge as they might limit the use of groundwater as safe drinking water even at ng L
MeSH term(s)	Child ; Humans ; Adolescent ; Child, Preschool ; Drinking Water ; Water Pollutants, Chemical/analysis ; Groundwater ; Water Resources ; Valsartan ; Environmental Monitoring
Chemical Substances	Drinking Water ; Water Pollutants, Chemical ; Valsartan (80M03YXJ7I)
Language	English
Publishing date	2023-08-30
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2023.140023
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Article ; Online: Improvement of electrochemical oxidation efficiency through combination with adsorption processes.

Muñoz-Morales, M / Sáez, C / Cañizares, P / Rodrigo, M A

Journal of environmental management

2020 Volume 262, Page(s) 110364

Abstract: In this work, a three-step process (adsorption-desorption-electrolysis) is evaluated as an interesting approach for the removal of organochlorinated compounds (clopyralid, lindane and perchloroethylene) with different physical properties (solubility and ... ...

Abstract	In this work, a three-step process (adsorption-desorption-electrolysis) is evaluated as an interesting approach for the removal of organochlorinated compounds (clopyralid, lindane and perchloroethylene) with different physical properties (solubility and vapor pressure) from low concentrated wastewater. First steps are based on the adsorptive capacity of granular active carbon (GAC) particles to retain organics and on the solvent capacity of methanol to extract them to concentrated solution and regenerate GAC. In the last step of electrolysis with conductive diamond electrodes, the degradation of pesticide is projected, as well as the recovery of methanol. Results show that clopyralid, lindane and PCE are efficiently retained in GAC, although adsorption efficiency depend on pollutant/GAC ratio and physicochemical properties of pollutant. Pretreatment allows the concentration of clopyralid and PCE solutions up to 8 times, but worse results are obtained in case of lindane solutions. Electrolysis of concentrated methanol solution seems to be more efficient than electrolysis of diluted aqueous wastes, mainly in the case of clopyralid. In all cases, electrochemical degradation fits a first order kinetics confirming mixed oxidation mechanisms with diffusion control of the direct processes and mediated oxidation. Results obtained in terms of current efficiency and energy consumption of electrolysis step point out the lower operation cost of concentrated liquid wastes and encourage further works on the development of cost-effective combined processes for the treatment of diluted solutions polluted with polar compounds (such as clopyralid).
MeSH term(s)	Adsorption ; Electrodes ; Electrolysis ; Oxidation-Reduction ; Waste Water ; Water Pollutants, Chemical
Chemical Substances	Waste Water ; Water Pollutants, Chemical
Language	English
Publishing date	2020-03-03
Publishing country	England
Document type	Journal Article
ZDB-ID	184882-3
ISSN	1095-8630 ; 0301-4797
ISSN (online)	1095-8630
ISSN	0301-4797
DOI	10.1016/j.jenvman.2020.110364
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Article ; Online: Deep continual learning for medical call incidents text classification under the presence of dataset shifts.

Ferri, Pablo / Lomonaco, Vincenzo / Passaro, Lucia C / Félix-De Castro, Antonio / Sánchez-Cuesta, Purificación / Sáez, Carlos / García-Gómez, Juan M

Computers in biology and medicine

2024 Volume 175, Page(s) 108548

Abstract: The aim of this work is to develop and evaluate a deep classifier that can effectively prioritize Emergency Medical Call Incidents (EMCI) according to their life-threatening level under the presence of dataset shifts. We utilized a dataset consisting of ... ...

Abstract	The aim of this work is to develop and evaluate a deep classifier that can effectively prioritize Emergency Medical Call Incidents (EMCI) according to their life-threatening level under the presence of dataset shifts. We utilized a dataset consisting of 1982746 independent EMCI instances obtained from the Health Services Department of the Region of Valencia (Spain), with a time span from 2009 to 2019 (excluding 2013). The dataset includes free text dispatcher observations recorded during the call, as well as a binary variable indicating whether the event was life-threatening. To evaluate the presence of dataset shifts, we examined prior probability shifts, covariate shifts, and concept shifts. Subsequently, we designed and implemented four deep Continual Learning (CL) strategies-cumulative learning, continual fine-tuning, experience replay, and synaptic intelligence-alongside three deep CL baselines-joint training, static approach, and single fine-tuning-based on DistilBERT models. Our results demonstrated evidence of prior probability shifts, covariate shifts, and concept shifts in the data. Applying CL techniques had a statistically significant (α=0.05) positive impact on both backward and forward knowledge transfer, as measured by the F1-score, compared to non-continual approaches. We can argue that the utilization of CL techniques in the context of EMCI is effective in adapting deep learning classifiers to changes in data distributions, thereby maintaining the stability of model performance over time. To our knowledge, this study represents the first exploration of a CL approach using real EMCI data.
MeSH term(s)	Humans ; Deep Learning ; Databases, Factual ; Spain ; Emergency Medical Services
Language	English
Publishing date	2024-05-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	127557-4
ISSN	1879-0534 ; 0010-4825
ISSN (online)	1879-0534
ISSN	0010-4825
DOI	10.1016/j.compbiomed.2024.108548
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