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  1. Article ; Online: Impact of Antithyroperoxidase Antibodies (Anti-TPO) on Ovarian Reserve and Early Embryo Development in Assisted Reproductive Technology Cycles.

    Safarian, Galina Kh / Niauri, Dariko A / Kogan, Igor Y / Bespalova, Olesya N / Dzhemlikhanova, Lyailya Kh / Lesik, Elena A / Komarova, Evgeniya M / Krikheli, Inna O / Obedkova, Ksenia V / Tkachenko, Nataliya N / Milyutina, Yulia P / Gzgzyan, Aleksandr M / Shoenfeld, Yehuda

    International journal of molecular sciences

    2023  Volume 24, Issue 5

    Abstract: Autoimmune thyroid disease (AITD) is one of the most common endocrinopathies and is more prevalent in women. It becomes evident that the circulating antithyroid antibodies that often follow AITD have effects on many tissues, including ovaries, and ... ...

    Abstract Autoimmune thyroid disease (AITD) is one of the most common endocrinopathies and is more prevalent in women. It becomes evident that the circulating antithyroid antibodies that often follow AITD have effects on many tissues, including ovaries, and therefore that this common morbidity might have an impact on female fertility, the investigation of which is the aim of the present research. Ovarian reserve, ovarian response to stimulation and early embryo development in infertile patients with thyroid autoimmunity were assessed in 45 women with thyroid autoimmunity and 45 age-matched control patients undergoing infertility treatment. It was demonstrated that the presence of anti-thyroid peroxidase antibodies is associated with lower serum anti-Müllerian hormone levels and antral follicle count. Further investigation revealed the higher prevalence of sub-optimal response to ovarian stimulation in TAI-positive women, lower fertilization rate and lower number of high-quality embryos in this group of patients. The cut-off value for follicular fluid anti-thyroid peroxidase antibody affecting the above-mentioned parameters was determined to be 105.0 IU/mL, highlighting the necessity of closer monitoring in couples seeking infertility treatment with ART.
    MeSH term(s) Female ; Humans ; Infertility, Female/etiology ; Infertility, Female/therapy ; Ovarian Reserve/immunology ; Embryonic Development/immunology ; Autoantibodies/immunology ; Iodide Peroxidase/immunology ; Thyroiditis, Autoimmune/complications ; Sperm Injections, Intracytoplasmic
    Chemical Substances anti-thyroid autoantibodies ; Autoantibodies ; TPO protein, human (EC 1.11.1.7) ; Iodide Peroxidase (EC 1.11.1.8)
    Language English
    Publishing date 2023-02-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24054705
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Local Immune Biomarker Expression Depending on the Uterine Microbiota in Patients with Idiopathic Infertility.

    Tapilskaya, Natalya I / Savicheva, Alevtina M / Shalepo, Kira V / Budilovskaya, Olga V / Gzgzyan, Aleksandr M / Bespalova, Olesya N / Khusnutdinova, Tatiana A / Krysanova, Anna A / Obedkova, Kseniia V / Safarian, Galina Kh

    International journal of molecular sciences

    2023  Volume 24, Issue 8

    Abstract: The endometrium has traditionally been considered sterile. Nowadays, active studies are performed on the female upper genital tract microbiota. Bacteria and/or viruses colonizing the endometrium are known to alter its functional properties, including ... ...

    Abstract The endometrium has traditionally been considered sterile. Nowadays, active studies are performed on the female upper genital tract microbiota. Bacteria and/or viruses colonizing the endometrium are known to alter its functional properties, including receptivity and embryo implantation. Uterine cavity inflammation caused by microorganisms leads to disrupted cytokine expression, which, in turn, is mandatory for the successful implantation of the embryo. The present study assessed the vaginal and endometrial microbiota composition and its relation to the levels of cytokines produced by the endometrium in reproductive-aged women complaining of secondary infertility of unknown origin. The multiplex real-time PCR assay was applied for vaginal and endometrial microbiota analysis. The quantitative measurement of endometrial α-defensin (DEFa1), transforming growth factor (TGFβ1), and basic fibroblast growth factor (bFGF2) was carried out using the ELISA (Cloud-Clone Corporation (Katy, TX, USA; manufactured in Wuhan, China). A reliable decline in endometrial TGFβ1 and bFGF2 and an increase in DEFa1 were demonstrated in women with idiopathic infertility when compared to fertile patients. However, TGFβ1, bFGF2, and DEFa1 expression correlated reliably only with the presence of
    MeSH term(s) Humans ; Female ; Adult ; Uterus/metabolism ; Infertility/metabolism ; Endometrium/metabolism ; Embryo Implantation ; Uterine Diseases ; Cytokines/metabolism ; Microbiota ; Infertility, Female/metabolism
    Chemical Substances Cytokines
    Language English
    Publishing date 2023-04-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24087572
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Anti-TPO-mediated specific features of the placenta immunohistochemical profile and possible mechanisms for fetal loss.

    Borodina, Elena / Gzgzyan, Alexander M / Dzhemlikhanova, Liailia Kh / Niauri, Dariko A / Tolibova, Gulrukhsor Kh / Tral, Tatiana G / Kogan, Igor Y / Safarian, Galina Kh / Ostrinski, Yuri / Churilov, Leonid P / Amital, Howard / Blank, Miri / Shoenfeld, Yehuda

    Clinical and experimental immunology

    2023  Volume 213, Issue 2, Page(s) 235–242

    Abstract: Passive transfer of antithyroid antibodies in mice leads to reproductive disorders. The purpose was to assess the placental tissue of experimental animals under the influence of the circulating thyroperoxidase antibodies. We performed an ... ...

    Abstract Passive transfer of antithyroid antibodies in mice leads to reproductive disorders. The purpose was to assess the placental tissue of experimental animals under the influence of the circulating thyroperoxidase antibodies. We performed an immunohistochemical examination of murine placentae after a passive transfer of thyroperoxidase antibodies. Placentae of mice that passively transferred IgG from healthy donors were used as control samples. For histological examination, 30 placental samples were selected from mice from the anti-TPO group and 40 placental samples were taken from mice from the IgG group. Immunostaining for VEGFR1, THBS 1, Laminin, CD31, CD34, FGF-β, CD56, CD14, TNF-α, kisspeptin, MCL 1, and Annexin V was performed. There is a significant decrease in the relative area of the expression of VEGFR1 (23.42 ± 0.85 vs. 33.44 ± 0.35, P < 0.01), thrombospondin 1 (31.29 ± 0.83 vs. 34.51 ± 0.75, P < 0.01), CD14 (25.80 ± 0.57 vs. 32.07 ± 0.36, P < .01), CD56 (30.08 ± 0.90 vs. 34.92 ± 0.15, P < 0.01), kisspeptin (25.94 ± 0.47 vs. 31.27 ± 0.57, P < 0.01), MCL 1 (29.24 ± 1.06 vs. 38.57 ± 0.79, P < 0.01) in the labyrinth zone of the placentae of mice from the anti-TPO group compared with control group. A significant increase in the relative expression of laminin and FGF-β was noted in the group of mice to which antibodies to thyroperoxidase were transferred, compared with the control group (36.73 ± 1.38 vs. 29.83 ± 0.94, P < 0.01 and 23.26 ± 0.61 vs. 16.38 ± 1.01, P < 0.01respectively). Our study exposed an imbalance of pro- and anti-angiogenic factors, decreased representation of placental macrophages and NK cells, abnormal trophoblast invasion processes, and insufficient expression of antiapoptotic factors in the placentae of mice in which anti-TPO antibodies were passively transferred.
    MeSH term(s) Pregnancy ; Female ; Animals ; Mice ; Placenta/pathology ; Laminin/metabolism ; Kisspeptins/metabolism ; Myeloid Cell Leukemia Sequence 1 Protein/metabolism ; Immunoglobulin G/metabolism
    Chemical Substances Laminin ; Kisspeptins ; Myeloid Cell Leukemia Sequence 1 Protein ; Immunoglobulin G
    Language English
    Publishing date 2023-05-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218531-3
    ISSN 1365-2249 ; 0009-9104 ; 0964-2536
    ISSN (online) 1365-2249
    ISSN 0009-9104 ; 0964-2536
    DOI 10.1093/cei/uxad057
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool.

    Savicheva, Alevtina M / Krysanova, Anna A / Budilovskaya, Olga V / Spasibova, Elena V / Khusnutdinova, Tatiana A / Shalepo, Kira V / Beliaeva, Natalia R / Safarian, Galina Kh / Sapozhnikov, Kirill V / Tapilskaya, Natalya I / Kogan, Igor Yu

    International journal of molecular sciences

    2023  Volume 24, Issue 21

    Abstract: Bacterial vaginosis (BV) is a most common microbiological syndrome. Multiplex next-generation sequencing (NGS) or molecular tests allow a complete and accurate vaginal microbiota profiling in order to determine the primary causative agent. Due to the ... ...

    Abstract Bacterial vaginosis (BV) is a most common microbiological syndrome. Multiplex next-generation sequencing (NGS) or molecular tests allow a complete and accurate vaginal microbiota profiling in order to determine the primary causative agent. Due to the high costs and limited availability of NGS, the multiplex real-time PCR draws more attention. The present study aimed to evaluate the microbial composition and dominant lactobacilli species in non-pregnant women with bacterial vaginosis using a multiplex RT-PCR test and determine its diagnostic significance. In total, 331 women complaining of vaginal discharge were included. BV was confirmed upon clinical examination and Nugent criteria. A real-time PCR test was carried out with a new Femoflor test, which identifies opportunistic bacteria, STD pathogens, and some viruses. According to the results, the rate of lactobacilli is significantly reduced in BV-affected patients when compared to healthy women. Moreover, the rate of
    MeSH term(s) Female ; Humans ; Gardnerella vaginalis/genetics ; Lactobacillus/genetics ; Microbiota/genetics ; Vagina/microbiology ; Vaginosis, Bacterial/diagnosis ; Vaginosis, Bacterial/microbiology
    Language English
    Publishing date 2023-11-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms242115880
    Database MEDical Literature Analysis and Retrieval System OnLINE

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