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Article ; Online: New Insights into Polymorphisms in Candidate Genes Associated with Incidence of Postparturient Endometritis in Ossimi Sheep (Ovis aries)

Safhi, Fatmah A. / Ateya, Ahmed

Agriculture. 2023 Dec. 15, v. 13, no. 12

2023

Abstract: This study examined the genes related to immunity, metabolism, and antioxidants that may interact with the prevalence of postpartum endometritis in Ossimi sheep. We used fifty endometritis-positive Ossimi sheep and fifty that appeared to be normal. For ... ...

Abstract	This study examined the genes related to immunity, metabolism, and antioxidants that may interact with the prevalence of postpartum endometritis in Ossimi sheep. We used fifty endometritis-positive Ossimi sheep and fifty that appeared to be normal. For the purpose of taking blood samples, each ewe had its jugular vein pierced. Nucleotide sequence differences for the immunological (alpha-2-macroglobulin, toll-like receptor 2, transforming growth factor beta, interleukin 1 receptor-associated kinase 3, high-mobility group box 1, Fc alpha and Mu receptor, and inducible nitric oxide synthase), metabolic (ADAM metallopeptidase with thrombospondin type 1 motif 20, potassium sodium-activated channel subfamily T member 2, Mitogen-activated protein kinase kinase kinase 4, FKBP prolyl isomerase 5, and relaxin family peptide receptor 1), and antioxidant (superoxide dismutase, catalase, NADH: ubiquinone oxidoreductase subunit s5, and Heme oxygenase-1) genes were found among sheep with endometritis and those in good condition utilizing PCR-DNA sequencing. Fisher’s exact test revealed a significant difference in the probability of dispersal of all significant nucleotide changes between ewe groups with and without endometritis (p ˂ 0.01). In endometritis ewes, there was a considerable up-regulation of the expression levels of A2M, TLR2, IRAK3, HMGB1, FCAMR, iNOS, ADAMTS20, KCNT2, MAP3K4, FKBP5, RXFP1, and HMOX1. Conversely, there was a down-regulation of the genes that encode TGF-β, SOD, CAT, and NDUFS5. The kind of marker and its frequency in postparturient endometrtits significantly impacted the transcript levels of the indicators under analysis. The results validate that nucleotide changes and gene manifestation outlines in these candidates are significant predictors of the prevalence of endometritis in sheep.
Keywords	Ovis aries ; Toll-like receptor 2 ; agriculture ; antioxidants ; blood ; catalase ; endometritis ; ewes ; genes ; heme oxygenase (biliverdin-producing) ; immunity ; inducible nitric oxide synthase ; interleukin-1 ; isomerases ; jugular vein ; metabolism ; mitogen-activated protein kinase kinase kinase ; nucleoproteins ; nucleotide sequences ; peptide receptors ; potassium ; probability ; relaxin ; superoxide dismutase ; ubiquinones
Language	English
Dates of publication	2023-1215
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2651678-0
ISSN	2077-0472
ISSN	2077-0472
DOI	10.3390/agriculture13122273
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Upregulated synthesis and production of bioactive compounds in Lotus arabicus L. by in vitro feeding with dried powder of date palm seeds.

Elsherif, Doaa E / Safhi, Fatmah A / Khalifa, Asmaa M / Ragab, Gehad A

BMC plant biology

2024 Volume 24, Issue 1, Page(s) 225

Abstract: Background: Plants are considered the primary source of many principal bioactive compounds that have been utilized in a wide range of applications including the pharmaceutical and biotechnological industries. Therefore, there is an imperative need to ... ...

Abstract	Background: Plants are considered the primary source of many principal bioactive compounds that have been utilized in a wide range of applications including the pharmaceutical and biotechnological industries. Therefore, there is an imperative need to modulate the production of natural bioactive components. The present study aimed to determine the importance of dried and pulverized date palm seeds (DPS) as a natural elicitor for the synthesis of secondary metabolites in Lotus arabicus L. Results: The presence of various antioxidant compounds, simple sugars, amino acids, fatty acids and reasonable mineral contents was distinct in the phytochemical characterization of DPS. The major components detected in DPS analysis were the 5-(hydroxymethyl) furfural and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyranone. The induced callus of L. arabicus (seven weeks old) was supplemented with DPS at different concentrations (0, 2, 4, 8 and 10 g/l) in culture media. Treatment with 8 g/l DPS induced the highest antioxidant capacity, ascorbic acid content and secondary metabolites (total phenolics and flavonoids) in the produced callus. Stress biomarkers (hydrogen peroxide and malondialdehyde) were found in the control ranges except at 10 g/l DPS. The expression patterns of key genes involoved in secondary metabolism modulation, such as phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavonol synthase (FLS) and deoxyxylulose phosphate reductoisomerase (DXR), were triggered after DPS treatments. Moreover, the quantitative profiling of phenolic and flavonoid compounds showed that supplementation with DPS, especially at 8 g/l, led to pronounced increases in most of the measured compounds. Conclusion: The marked upregulation of eliciting-responsive genes and overproduction of secondary metabolites provide molecular-based evidence for intensifying the principal pathways of phenylpropanoid, flavonoid and terpenoid biosynthesis. Overall, the present in vitro study highlights the stimulating capacity of DPS utilization to improve the bioactive components of L. arabicus at the physiological and molecular levels, enhancing its potential as a medicinal herb.
MeSH term(s)	Antioxidants/metabolism ; Lotus/metabolism ; Phoeniceae/metabolism ; Powders ; Flavonoids/metabolism ; Phenols/metabolism ; Seeds/metabolism
Chemical Substances	Antioxidants ; Powders ; Flavonoids ; Phenols
Language	English
Publishing date	2024-03-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2059868-3
ISSN	1471-2229 ; 1471-2229
ISSN (online)	1471-2229
ISSN	1471-2229
DOI	10.1186/s12870-024-04919-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: DNA Polymorphisms and mRNA Levels of Immune Biomarkers as Candidates for Inflammatory Postpartum Disorders Susceptibility in Italian Buffaloes.

Ateya, Ahmed / Safhi, Fatmah A / El-Emam, Huda / Al-Ghadi, Muath Q / Abdo, Mohamed / Fericean, Liana / Olga, Rada / Mihaela, Ostan / Hizam, Manar M / Mamdouh, Maha / Abu El-Naga, Eman M / Raslan, Walaa S

Veterinary sciences

2023 Volume 10, Issue 9

Abstract: The immunological genes that may interact with inflammatory postpartum diseases in Italian buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal and 60 with inflammatory reproductive diseases) were employed. ... ...

Abstract	The immunological genes that may interact with inflammatory postpartum diseases in Italian buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal and 60 with inflammatory reproductive diseases) were employed. Each buffalo's jugular vein was pierced to get five milliliters of blood. To obtain whole blood and extract DNA and RNA, the blood was placed within tubes containing sodium fluoride or EDTA anticoagulants. The immunological (
Language	English
Publishing date	2023-09-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2768971-2
ISSN	2306-7381 ; 2306-7381
ISSN (online)	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci10090573
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Assessment of intra- and inter-genetic diversity in tetraploid and hexaploid wheat genotypes based on omega, gamma and alpha-gliadin profiles.

Al-Khayri, Jameel M / Alwutayd, Khairiah M / Safhi, Fatmah A / Alqahtani, Mesfer M / Alshegaihi, Rana M / Abd El-Moneim, Diaa / Jain, Shri Mohan / Eldomiaty, Ahmed S / Alshamrani, Rahma / Abuzaid, Amani Omar / Hassanin, Abdallah A

PeerJ

2023 Volume 11, Page(s) e16330

Abstract: Durum and bread wheat are well adapted to the Mediterranean Basin. Twenty-three genotypes of each species were grown to evaluate the intra- and inter-genetic diversity based on omega ( ...

Abstract	Durum and bread wheat are well adapted to the Mediterranean Basin. Twenty-three genotypes of each species were grown to evaluate the intra- and inter-genetic diversity based on omega (
MeSH term(s)	Gliadin/genetics ; Triticum/genetics ; Tetraploidy ; Glutamine/genetics ; Genotype ; Proline/genetics
Chemical Substances	Gliadin (9007-90-3) ; Glutamine (0RH81L854J) ; Proline (9DLQ4CIU6V)
Language	English
Publishing date	2023-11-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	2703241-3
ISSN	2167-8359 ; 2167-8359
ISSN (online)	2167-8359
ISSN	2167-8359
DOI	10.7717/peerj.16330
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Gamma-rays induced mutations increase soybean oil and protein contents.

Mohsen, Geehan / Soliman, Said S / Mahgoub, Elsayed I / Ismail, Tarik A / Mansour, Elsayed / Alwutayd, Khairiah M / Safhi, Fatmah A / Abd El-Moneim, Diaa / Alshamrani, Rahma / Atallah, Osama O / Shehata, Wael F / Hassanin, Abdallah A

PeerJ

2023 Volume 11, Page(s) e16395

Abstract: Mutation breeding is one of the effective techniques used for improving desired traits such as yield quality and quantity in economic crops. The present study aims to develop oil and protein contents in addition to high yield attributes in soybean using ... ...

Abstract	Mutation breeding is one of the effective techniques used for improving desired traits such as yield quality and quantity in economic crops. The present study aims to develop oil and protein contents in addition to high yield attributes in soybean using gamma rays as a mutagen. Seeds of the soybean genotypes Giza 21, Giza 22, Giza 82, Giza 83 and 117 were treated with gamma rays doses 50, 100, 200 and 300 Gy. Plants were then scored based on morphological parameters correlated with yield quantity including plant height, seed weight and valuable protein and oil contents. Mutant lines exhibiting the highest yield attributes were selected and used as parents for M2 generation. The M2 progeny was further assessed based on their ability to maintain their yield attributes. Twenty mutant lines were selected and used as M3 lines. The yield parameters inferred a positive effect of gamma irradiation on the collected M3 mutant lines compared to their parental genotypes. 100 Gy of gamma rays gave the highest effect on the number of pods, branches and seeds per plant in addition to protein content, while 200 Gy was more effective in increasing plant height, number of pods per plant, and oil content. Six mutant lines scored the highest yield parameters. Further assessment inferred an inverse relationship between oil and protein content in most of the tested cultivars with high agronomic features. However, four mutant lines recorded high content of oil and protein besides their high seed yield as well, which elect them as potential candidates for large-scale evaluation. The correlation among examined parameters was further confirmed
MeSH term(s)	Soybean Oil/metabolism ; Gamma Rays ; Plant Breeding ; Glycine max/genetics ; Mutation
Chemical Substances	Soybean Oil (8001-22-7)
Language	English
Publishing date	2023-11-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	2703241-3
ISSN	2167-8359 ; 2167-8359
ISSN (online)	2167-8359
ISSN	2167-8359
DOI	10.7717/peerj.16395
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Exploring genotypic variability and interrelationships among growth, yield, and quality characteristics in diverse tomato genotypes.

Zannat, Arova / Hussain, Md Arif / Md Abdullah, Abu Habib / Hossain, Md Ismail / Saifullah, Md / Safhi, Fatmah A / Alshallash, Khalid S / Mansour, Elsayed / ElSayed, Abdelaleim I / Hossain, Md Sazzad

Heliyon

2023 Volume 9, Issue 8, Page(s) e18958

Abstract: Tomato is the most consumed vegetable crop worldwide, with excellent beneficial health properties and high content of vitamins, minerals, carotenoids, total antioxidants, and phenolic compounds. Hence, improving its genotypes is crucial to sustain its ... ...

Abstract	Tomato is the most consumed vegetable crop worldwide, with excellent beneficial health properties and high content of vitamins, minerals, carotenoids, total antioxidants, and phenolic compounds. Hence, improving its genotypes is crucial to sustain its production and ensure food security, principally under the fast-growing worldwide population and abrupt global climate change. The present study aimed to explore the genotypic variability associated with specific characteristics in twenty-five diverse tomato genotypes. In addition, the relationships between growth, yield, and quality traits using both univariate (correlation coefficient, path analysis) and multivariate (principal component, principal coordinates, canonical variate) analysis methods were explored. The results indicated that the evaluated genotypes possessed highly significant variation. This is appropriate for future hybridization through tomato breeding programs. All evaluated genotypes demonstrated considerable potential to develop strong hybrid vigour for growth, yield, and quality characteristics. In particular, the genotypes LS009, LS011, and LS014 could be considered promising, high-yielding, and resistant to yellow leaf curl virus infestation (YLCV) disease parents for future breeding schemes. The number of fruits per plant, fruit diameter, and fruit weight proved strong positive relationships with fruit yield. Accordingly, these characteristics demonstrate their importance in improving fruit yield and could be exploited as indirect criteria for selecting high-yielding tomato genotypes through breeding programs.
Language	English
Publishing date	2023-08-04
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e18958
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Molecular Genetic Diversity of Local and Exotic Durum Wheat Genotypes and Their Combining Ability for Agronomic Traits under Water Deficit and Well-Watered Conditions.

Galal, Ahmed A / Safhi, Fatmah A / El-Hity, Mahmoud A / Kamara, Mohamed M / Gamal El-Din, Eman M / Rehan, Medhat / Farid, Mona / Behiry, Said I / El-Soda, Mohamed / Mansour, Elsayed

Life (Basel, Switzerland)

2023 Volume 13, Issue 12

Abstract: Water deficit poses significant environmental stress that adversely affects the growth and productivity of durum wheat. Moreover, projections of climate change suggest an increase in the frequency and severity of droughts, particularly in arid regions. ... ...

Abstract	Water deficit poses significant environmental stress that adversely affects the growth and productivity of durum wheat. Moreover, projections of climate change suggest an increase in the frequency and severity of droughts, particularly in arid regions. Consequently, there is an urgent need to develop drought-tolerant and high-yielding genotypes to ensure sustained production and global food security in response to population growth. This study aimed to explore the genetic diversity among local and exotic durum wheat genotypes using simple sequence repeat (SSR) markers and, additionally, to explore the combining ability and agronomic performance of assessed durum wheat genotypes and their 28 F
Language	English
Publishing date	2023-12-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662250-6
ISSN	2075-1729
ISSN	2075-1729
DOI	10.3390/life13122293
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels

Ateya, Ahmed / Safhi, Fatmah A. / El-Emam, Huda / Marawan, Marawan A. / Fayed, Hayat / Kadah, Amgad / Mamdouh, Maha / Hizam, Manar M. / Al-Ghadi, Muath Q. / Abdo, Mohamed / Fericean, Liana / Olga, Rada / Mihaela, Ostan

Agriculture. 2023 Sept. 28, v. 13, no. 10

2023

Abstract: The immune and antioxidant genetic factors that could converse with mastitis susceptibility in dromedary camels were looked at in this research. Of 120 female dromedary camels (60 healthy, and 60 with mastitis) were utilised. Each camel’s jugular vein ... ...

Abstract	The immune and antioxidant genetic factors that could converse with mastitis susceptibility in dromedary camels were looked at in this research. Of 120 female dromedary camels (60 healthy, and 60 with mastitis) were utilised. Each camel’s jugular vein was pierced to obtain five millilitres of blood. The blood was placed within tubes containing sodium fluoride or EDTA anticoagulants to obtain whole blood and extract DNA and RNA. The immunological (OTUD3, TLR2, TLR4, STAB2, MBL2, TRAPPC9, and C4A) and antioxidant (CAT, SOD3, PRDX6, OXSR1, NDUFS6, SERP2, and ST1P1) genes’ nucleotide sequence polymorphisms between healthy and mastitis affected she-camels were discovered using PCR-DNA sequencing. Fisher’s exact test revealed that camel groups with and without mastitis had noticeably different odds of all major nucleotide alterations propagating (p < 0.01). Mastitic camels were significantly more likely to express the OTUD3, TLR2, TLR4, STAB2, MBL2, TRAPPC9, C4A, OXSR1, SERP2, and ST1P1 genes (p < 0.05). However, CAT, SOD3, PRDX6, and NDUFS6 genes elicited a different pattern. The results may be used to develop management strategies and support the significance of nucleotide differences and gene expression patterns in these markers as indicators of the incidence of mastitis.
Keywords	Camelus dromedarius ; DNA ; RNA ; agriculture ; antioxidants ; blood ; camels ; females ; gene expression ; jugular vein ; mastitis ; nucleotide sequences ; sodium fluoride
Language	English
Dates of publication	2023-0928
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2651678-0
ISSN	2077-0472
ISSN	2077-0472
DOI	10.3390/agriculture13101909
Database	NAL-Catalogue (AGRICOLA)

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Article: Green Synthesized of

Abd-Ellatif, Sawsan / Ibrahim, Amira A / Safhi, Fatmah A / Abdel Razik, Elsayed S / Kabeil, Sanaa S A / Aloufi, Salman / Alyamani, Amal A / Basuoni, Mostafa M / ALshamrani, Salha Mesfer / Elshafie, Hazem S

Plants (Basel, Switzerland)

2022 Volume 11, Issue 22

Abstract: ... Fusarium ... ...

Abstract	Fusarium solani
Language	English
Publishing date	2022-11-16
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants11223129
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Impact of Green Chitosan Nanoparticles Fabricated from Shrimp Processing Waste as a Source of Nano Nitrogen Fertilizers on the Yield Quantity and Quality of Wheat (

Saad, Ahmed M / Alabdali, Aya Yaseen Mahmood / Ebaid, Mohamed / Salama, Eslam / El-Saadony, Mohamed T / Selim, Samy / Safhi, Fatmah A / ALshamrani, Salha M / Abdalla, Hanan / Mahdi, Ayman H A / El-Saadony, Fathy M A

Molecules (Basel, Switzerland)

2022 Volume 27, Issue 17

Abstract: Waste from crustaceans has adverse effects on the environment. In this respect, shrimp waste was valorized for producing chitosan nanoparticles as a source for eco-friendly nano-nitrogen fertilizer. The application of nano-nitrogen fertilizers is a ... ...

Abstract	Waste from crustaceans has adverse effects on the environment. In this respect, shrimp waste was valorized for producing chitosan nanoparticles as a source for eco-friendly nano-nitrogen fertilizer. The application of nano-nitrogen fertilizers is a valuable alternative approach in agriculture due to its potential for reducing the application of mineral nitrogen fertilizers and increasing yield quality and quantity, thereby helping to reduce the worldwide food shortage. Chitosan nanoparticles were foliar sprayed at three volumes (0, 7, and 14 L/ha) and compared with mineral nitrogen fertilizer (M-N) sprayed at three volumes (0, 120, and 240 kg N/ha) and their combination on two wheat cultivars (Misr-1 and Gemaiza-11) during two consecutive seasons (2019/2020 and 2020/2021) in order to evaluate the agronomic response. The synthesized chitosan nanoparticles displayed characteristic bands of both Nan-N and urea/chitosan from 500-4000 cm
MeSH term(s)	Agriculture/methods ; Chitosan ; Chlorophyll ; Edible Grain/chemistry ; Fertilizers/analysis ; Nanoparticles ; Nitrogen/analysis ; Potassium ; Soil ; Triticum
Chemical Substances	Fertilizers ; Soil ; Chlorophyll (1406-65-1) ; Chitosan (9012-76-4) ; Nitrogen (N762921K75) ; Potassium (RWP5GA015D)
Language	English
Publishing date	2022-09-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules27175640
Database	MEDical Literature Analysis and Retrieval System OnLINE

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