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  1. Article ; Online: Biomaterials for recruiting and activating endogenous stem cells in situ tissue regeneration.

    Safina, Ingrid / Embree, Mildred C

    Acta biomaterialia

    2022  Volume 143, Page(s) 26–38

    Abstract: Over the past two decades in situ tissue engineering has emerged as a new approach where biomaterials are used to harness the body's own stem/progenitor cells to regenerate diseased or injured tissue. Immunomodulatory biomaterials are designed to promote ...

    Abstract Over the past two decades in situ tissue engineering has emerged as a new approach where biomaterials are used to harness the body's own stem/progenitor cells to regenerate diseased or injured tissue. Immunomodulatory biomaterials are designed to promote a regenerative environment, recruit resident stem cells to diseased or injured tissue sites, and direct them towards tissue regeneration. This review explores advances gathered from in vitro and in vivo studies on in situ tissue regenerative therapies. Here we also examine the different ways this approach has been incorporated into biomaterial sciences in order to create customized biomaterial products for therapeutic applications in a broad spectrum of tissues and diseases. STATEMENT OF SIGNIFICANCE: Biomaterials can be designed to recruit stem cells and coordinate their behavior and function towards the restoration or replacement of damaged or diseased tissues in a process known as in situ tissue regeneration. Advanced biomaterial constructs with precise structure, composition, mechanical, and physical properties can be transplanted to tissue site and exploit local stem cells and their micro-environment to promote tissue regeneration. In the absence of cells, we explore the critical immunomodulatory, chemical and physical properties to consider in material design and choice. The application of biomaterials for in situ tissue regeneration has the potential to address a broad range of injuries and diseases.
    MeSH term(s) Biocompatible Materials/pharmacology ; Stem Cells ; Tissue Engineering ; Wound Healing
    Chemical Substances Biocompatible Materials
    Language English
    Publishing date 2022-03-12
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2173841-5
    ISSN 1878-7568 ; 1742-7061
    ISSN (online) 1878-7568
    ISSN 1742-7061
    DOI 10.1016/j.actbio.2022.03.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Cell-Biomaterial constructs for wound healing and skin regeneration.

    Safina, Ingrid / Childress, Luke T / Myneni, Srinivas R / Vang, Kieng Bao / Biris, Alexandru S

    Drug metabolism reviews

    2022  Volume 54, Issue 1, Page(s) 63–94

    Abstract: Over the years, conventional skin grafts, such as full-thickness, split-thickness, and pre-sterilized grafts from human or animal sources, have been at the forefront of skin wound care. However, these conventional grafts are associated with major ... ...

    Abstract Over the years, conventional skin grafts, such as full-thickness, split-thickness, and pre-sterilized grafts from human or animal sources, have been at the forefront of skin wound care. However, these conventional grafts are associated with major challenges, including supply shortage, rejection by the immune system, and disease transmission following transplantation. Due to recent progress in nanotechnology and material sciences, advanced artificial skin grafts-based on the fundamental concepts of tissue engineering-are quickly evolving for wound healing and regeneration applications, mainly because they can be uniquely tailored to meet the requirements of specific injuries. Despite tremendous progress in tissue engineering, many challenges and uncertainties still face skin grafts
    MeSH term(s) Animals ; Biocompatible Materials ; Humans ; Skin ; Skin Transplantation ; Skin, Artificial ; Tissue Engineering ; Wound Healing
    Chemical Substances Biocompatible Materials
    Language English
    Publishing date 2022-04-02
    Publishing country England
    Document type Journal Article ; Review ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
    ZDB-ID 184967-0
    ISSN 1097-9883 ; 0360-2532 ; 0012-6594
    ISSN (online) 1097-9883
    ISSN 0360-2532 ; 0012-6594
    DOI 10.1080/03602532.2021.2025387
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1030: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  3. Article ; Online: Lgr5-expressing secretory cells form a Wnt inhibitory niche in cartilage critical for chondrocyte identity.

    Ruscitto, Angela / Chen, Peng / Tosa, Ikue / Wang, Ziyi / Zhou, Gan / Safina, Ingrid / Wei, Ran / Morel, Mallory M / Koch, Alia / Forman, Michael / Reeve, Gwendolyn / Lecholop, Michael K / Wilson, Marshall / Bonthius, Daniel / Chen, Mo / Ono, Mitsuaki / Wang, Timothy C / Yao, Hai / Embree, Mildred C

    Cell stem cell

    2023  Volume 30, Issue 9, Page(s) 1179–1198.e7

    Abstract: Osteoarthritis is a degenerative joint disease that causes pain, degradation, and dysfunction. Excessive canonical Wnt signaling in osteoarthritis contributes to chondrocyte phenotypic instability and loss of cartilage homeostasis; however, the ... ...

    Abstract Osteoarthritis is a degenerative joint disease that causes pain, degradation, and dysfunction. Excessive canonical Wnt signaling in osteoarthritis contributes to chondrocyte phenotypic instability and loss of cartilage homeostasis; however, the regulatory niche is unknown. Using the temporomandibular joint as a model in multiple species, we identify Lgr5-expressing secretory cells as forming a Wnt inhibitory niche that instruct Wnt-inactive chondroprogenitors to form the nascent synovial joint and regulate chondrocyte lineage and identity. Lgr5 ablation or suppression during joint development, aging, or osteoarthritis results in depletion of Wnt-inactive chondroprogenitors and a surge of Wnt-activated, phenotypically unstable chondrocytes with osteoblast-like properties. We recapitulate the cartilage niche and create StemJEL, an injectable hydrogel therapy combining hyaluronic acid and sclerostin. Local delivery of StemJEL to post-traumatic osteoarthritic jaw and knee joints in rabbit, rat, and mini-pig models restores cartilage homeostasis, chondrocyte identity, and joint function. We provide proof of principal that StemJEL preserves the chondrocyte niche and alleviates osteoarthritis.
    MeSH term(s) Swine ; Animals ; Rabbits ; Rats ; Chondrocytes ; Swine, Miniature ; Cartilage ; Aging ; Receptors, G-Protein-Coupled ; Osteoarthritis
    Chemical Substances Receptors, G-Protein-Coupled
    Language English
    Publishing date 2023-09-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2375354-7
    ISSN 1875-9777 ; 1934-5909
    ISSN (online) 1875-9777
    ISSN 1934-5909
    DOI 10.1016/j.stem.2023.08.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6589: Show issues Location:
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    Zs.MG 75: Show issues

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  4. Article ; Online: Graphene-based 2D constructs for enhanced fibroblast support.

    Safina, Ingrid / Bourdo, Shawn E / Algazali, Karrer M / Kannarpady, Ganesh / Watanabe, Fumiya / Vang, Kieng Bao / Biris, Alexandru S

    PloS one

    2020  Volume 15, Issue 5, Page(s) e0232670

    Abstract: Complex skin wounds have always been a significant health and economic problem worldwide due to their elusive and sometimes poor or non-healing conditions. If not well-treated, such wounds may lead to amputation, infections, cancer, or even death. Thus, ... ...

    Abstract Complex skin wounds have always been a significant health and economic problem worldwide due to their elusive and sometimes poor or non-healing conditions. If not well-treated, such wounds may lead to amputation, infections, cancer, or even death. Thus, there is a need to efficiently generate multifunctional skin grafts that address a wide range of skin conditions, including non-healing wounds, and enable the regeneration of new skin tissue. Here, we propose studying pristine graphene and two of its oxygen-functionalized derivatives-high and low-oxygen graphene films-as potential substrates for skin cell proliferation and differentiation. Using BJ cells (human foreskin-derived fibroblasts) to represent basic skin cells, we show that the changes in surface properties of pristine graphene due to oxygen functionalization do not seem to statistically impact the normal proliferation and maturation of skin cells. Our results indicate that the pristine and oxidized graphenes presented relatively low cytotoxicity to BJ fibroblasts and, in fact, support their growth and bioactivity. Therefore, these graphene films could potentially be integrated into more complex skin regenerative systems to support skin regeneration. Because graphene's surface can be relatively easily functionalized with various chemical groups, this finding presents a major opportunity for the development of various composite materials that can act as active components in regenerative applications such as skin regeneration.
    MeSH term(s) Cell Line ; Cell Proliferation ; Cell Survival ; Fibroblasts/cytology ; Graphite/chemistry ; Humans ; Surface Properties ; Tissue Engineering ; Tissue Scaffolds/chemistry
    Chemical Substances graphene oxide ; Graphite (7782-42-5)
    Language English
    Publishing date 2020-05-18
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0232670
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Gold nanorods enhance different immune cells and allow for efficient targeting of CD4+ Foxp3+ Tregulatory cells.

    Safina, Ingrid / Al Sudani, Zeid A Nima / Hashoosh, Ahmed / Darrigues, Emilie / Watanabe, Fumiya / Biris, Alexandru S / Dings, Ruud P M / Bao Vang, Kieng

    PloS one

    2021  Volume 16, Issue 8, Page(s) e0241882

    Abstract: Gold nanoparticles (AuNPs) hold great promise in nanomedicine, yet their successful clinical translation has not been realized. Some challenges include effective AuNP targeting and delivery to improve modulation of immune cells of interest while limiting ...

    Abstract Gold nanoparticles (AuNPs) hold great promise in nanomedicine, yet their successful clinical translation has not been realized. Some challenges include effective AuNP targeting and delivery to improve modulation of immune cells of interest while limiting potential adverse effects. In order to overcome these challenges, we must fully understand how AuNPs impact different immune cell subsets, particularly within the dendritic cell and T cell compartments. Herein, we show that polyethylene glycol coated (PEG) gold nanorods (AuNRs) and PEG AuNRs covered with a thin layer of silver (AuNR/Ag) may enhance the immune response towards immune suppression or activation. We also studied the ability to enhance CD4+ Foxp3+ Tregs in vitro using AuNRs functionalized with interleukin 2 (IL2), a cytokine that is important in Treg development and homeostasis. Our results indicate that AuNRs enhance different immune cells and that NP composition matters in immune targeting. This knowledge will help us understand how to better design AuNRs to target and enhance the immune system.
    MeSH term(s) Animals ; CD4-Positive T-Lymphocytes/drug effects ; CD4-Positive T-Lymphocytes/metabolism ; Dendritic Cells/drug effects ; Dendritic Cells/metabolism ; Forkhead Transcription Factors/metabolism ; Gold/pharmacology ; Immune System/drug effects ; Immune System/metabolism ; Immunity/drug effects ; Interleukin-2/metabolism ; Metal Nanoparticles/administration & dosage ; Mice ; Mice, Inbred C57BL ; Nanotubes/chemistry ; Polyethylene Glycols/chemistry ; Silver/administration & dosage
    Chemical Substances Forkhead Transcription Factors ; Foxp3 protein, mouse ; Interleukin-2 ; Silver (3M4G523W1G) ; Polyethylene Glycols (3WJQ0SDW1A) ; Gold (7440-57-5)
    Language English
    Publishing date 2021-08-30
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0241882
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Dendritic cell biocompatibility of ether-based urethane films.

    Safina, Ingrid / Alghazali, Karrer M / Childress, Luke / Griffin, Christopher / Hashoosh, Ahmed / Kannarpady, Ganesh / Watanabe, Fumiya / Bourdo, Shawn E / Dings, Ruud P M / Biris, Alexandru S / Vang, Kieng Bao

    Journal of applied toxicology : JAT

    2021  Volume 41, Issue 9, Page(s) 1456–1466

    Abstract: The use of synthetic materials for biomedical applications is ever expanding. One of the major requirements for these materials is biocompatibility, which includes prevention of immune system responses. Due to the inherent complexity of their structural ... ...

    Abstract The use of synthetic materials for biomedical applications is ever expanding. One of the major requirements for these materials is biocompatibility, which includes prevention of immune system responses. Due to the inherent complexity of their structural composition, the polyurethane (PU) family of polymers is being used in a variety of medical applications, from soft and hard tissue scaffolds to intricate coatings on implantable devices. Herein, we investigated whether two polymer materials, D3 and D7, induced an immune response, measured by their effects on a dendritic cell (DC) line, JAWS II. Using a lactate dehydrogenase cytotoxicity assay and Annexin V/PI staining, we found that the PU materials did not induce cytotoxicity in DC cells. Using confocal microscopy, we also showed that the materials did not induce activation or maturation, as compared to positive controls. This was confirmed by looking at various markers, CD80, CD86, MHC class I, and MHC class II, via flow cytometry. Overall, the results indicated that the investigated PU films are biocompatible in terms of immunotoxicology and immunogenicity and show great promise for use in regenerative medicine.
    MeSH term(s) Animals ; Biocompatible Materials ; Bone Marrow Cells/drug effects ; Cell Survival/drug effects ; Dendritic Cells/drug effects ; Dendritic Cells/immunology ; Ethers ; Materials Testing/methods ; Mice ; Mice, Inbred C57BL ; Nanostructures/toxicity ; Polyurethanes/pharmacology ; Regenerative Medicine ; Tissue Engineering ; Tissue Scaffolds
    Chemical Substances Biocompatible Materials ; Ethers ; Polyurethanes
    Language English
    Publishing date 2021-01-08
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 604625-3
    ISSN 1099-1263 ; 0260-437X
    ISSN (online) 1099-1263
    ISSN 0260-437X
    DOI 10.1002/jat.4136
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1661: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Modifying Dendritic Cell Activation with Plasmonic Nano Vectors.

    Vang, Kieng Bao / Safina, Ingrid / Darrigues, Emilie / Nedosekin, Dmitry / Nima, Zeid A / Majeed, Waqar / Watanabe, Fumiya / Kannarpady, Ganesh / Kore, Rajshekhar A / Casciano, Daniel / Zharov, Vladimir P / Griffin, Robert J / Dings, Ruud P M / Biris, Alexandru S

    Scientific reports

    2017  Volume 7, Issue 1, Page(s) 5513

    Abstract: Dendritic cells (DCs) can acquire, process, and present antigens to T-cells to induce an immune response. For this reason, targeting cancer antigens to DCs in order to cause an immune response against cancer is an emerging area of nanomedicine that has ... ...

    Abstract Dendritic cells (DCs) can acquire, process, and present antigens to T-cells to induce an immune response. For this reason, targeting cancer antigens to DCs in order to cause an immune response against cancer is an emerging area of nanomedicine that has the potential to redefine the way certain cancers are treated. The use of plasmonically active silver-coated gold nanorods (henceforth referred to as plasmonic nano vectors (PNVs)) as potential carriers for DC tumor vaccines has not been presented before. Effective carriers must be able to be phagocytized by DCs, present low toxicity, and induce the maturation of DCs-an early indication of an immune response. When we treated DCs with the PNVs, we found that the cell viability of DCs was unaffected, up to 200 μg/ml. Additionally, the PNVs associated with the DCs as they were phagocytized and they were found to reside within intracellular compartments such as endosomes. More importantly, the PNVs were able to induce expression of surface markers indicative of DC activation and maturation, i.e. CD40, CD86, and MHC class II. These results provide the first evidence that PNVs are promising carriers for DC-based vaccines and warrant further investigating for clinical use.
    MeSH term(s) Animals ; B7-2 Antigen/metabolism ; CD40 Antigens/metabolism ; Cell Differentiation/drug effects ; Cell Line ; Cell Survival/drug effects ; Dendritic Cells/cytology ; Dendritic Cells/drug effects ; Dendritic Cells/immunology ; Gold/pharmacology ; Histocompatibility Antigens Class II/pharmacology ; Metal Nanoparticles/chemistry ; Mice ; Nanotubes/chemistry ; Phagocytosis ; Silver/pharmacology
    Chemical Substances B7-2 Antigen ; CD40 Antigens ; Cd86 protein, mouse ; Histocompatibility Antigens Class II ; Silver (3M4G523W1G) ; Gold (7440-57-5)
    Language English
    Publishing date 2017-07-14
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-04459-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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