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Article ; Online: TACC3: a multi-functional protein promoting cancer cell survival and aggressiveness.

Saatci, Ozge / Sahin, Ozgur

2024 Volume 22, Issue 23-24, Page(s) 2637–2655

Abstract: TACC3 is the most oncogenic member of the transforming acidic coiled-coil domain-containing protein (TACC) family. It is one of the major recruitment factors of distinct multi-protein complexes. TACC3 is localized to spindles, centrosomes, and nucleus, ... ...

Abstract	TACC3 is the most oncogenic member of the transforming acidic coiled-coil domain-containing protein (TACC) family. It is one of the major recruitment factors of distinct multi-protein complexes. TACC3 is localized to spindles, centrosomes, and nucleus, and regulates key oncogenic processes, including cell proliferation, migration, invasion, and stemness. Recently, TACC3 inhibition has been identified as a vulnerability in highly aggressive cancers, such as cancers with centrosome amplification (CA). TACC3 has spatiotemporal functions throughout the cell cycle; therefore, targeting TACC3 causes cell death in mitosis and interphase in cancer cells with CA. In the clinics, TACC3 is highly expressed and associated with worse survival in multiple cancers. Furthermore, TACC3 is a part of one of the most common fusions of FGFR, FGFR3-TACC3 and is important for the oncogenicity of the fusion. A detailed understanding of the regulation of TACC3 expression, its key partners, and molecular functions in cancer cells is vital for uncovering the most vulnerable tumors and maximizing the therapeutic potential of targeting this highly oncogenic protein. In this review, we summarize the established and emerging interactors and spatiotemporal functions of TACC3 in cancer cells, discuss the potential of TACC3 as a biomarker in cancer, and therapeutic potential of its inhibition.
MeSH term(s)	Humans ; Microtubule-Associated Proteins/metabolism ; Cell Survival ; Neoplasms/genetics ; Neoplasms/metabolism ; Centrosome/metabolism ; Cell Proliferation/genetics ; Cell Cycle Proteins/metabolism
Chemical Substances	Microtubule-Associated Proteins ; Cell Cycle Proteins ; TACC3 protein, human
Language	English
Publishing date	2024-01-10
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, N.I.H., Extramural
ZDB-ID	2146183-1
ISSN	1551-4005 ; 1538-4101 ; 1554-8627
ISSN (online)	1551-4005
ISSN	1538-4101 ; 1554-8627
DOI	10.1080/15384101.2024.2302243
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Article ; Online: Genetically Intact Bioengineered Spores of

Quijano, Juan F / Sahin, Ozgur

ACS synthetic biology

2021 Volume 10, Issue 4, Page(s) 778–785

Abstract: Developments in genome editing offer potential solutions to challenges in agriculture, industry, medicine, and the environment. However, many technologies remain unexploited due to limitations in the use of genetically altered organisms. In this study, ... ...

Abstract	Developments in genome editing offer potential solutions to challenges in agriculture, industry, medicine, and the environment. However, many technologies remain unexploited due to limitations in the use of genetically altered organisms. In this study, we use
MeSH term(s)	Bacillus subtilis/genetics ; Bacillus subtilis/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Gene Editing ; Gene Expression Regulation, Bacterial/genetics ; Gene Expression Regulation, Bacterial/physiology ; Spores, Bacterial/genetics ; Spores, Bacterial/metabolism
Chemical Substances	Bacterial Proteins
Language	English
Publishing date	2021-03-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2161-5063
ISSN (online)	2161-5063
DOI	10.1021/acssynbio.0c00578
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Article: Foliar Zinc Sprays Affected Yield and Bioactive Compounds of Granny Smith Apple

Erdem, Halil / Sahin, Ozgur

International journal of fruit science. 2021 Jan. 01, v. 21, no. 1

2021

Abstract: In this study, effects of foliar Zn sprays at different periods on yield, fruit quality attributes, bioactive compounds and micro-macro elements of ‘Granny Smith’ (Malus x domestica Borkh.) apples grafted on MM111 rootstocks were investigated. ... ...

Abstract	In this study, effects of foliar Zn sprays at different periods on yield, fruit quality attributes, bioactive compounds and micro-macro elements of ‘Granny Smith’ (Malus x domestica Borkh.) apples grafted on MM111 rootstocks were investigated. Experimental treatments were arranged as control (without Zn sprays), Zn-1 [Zn sprays 1 month after full bloom (when the fruit had a size of a walnut)], Zn-2 (Zn sprays 1 and 2 months after full bloom) and Zn-3 (Zn sprays 1, 2 and 3 months after full bloom). Experimental trees were sprayed with 0.2% Zn (ZnSO₄.7H₂O). Compared to control, Zn sprays increased yields. SSC values of Zn-2 and Zn-3 treatments were lower than the control and Zn-1 treatments. The total phenolics and total flavonoids of Zn-treated fruit were lower than the control fruit. The greatest vitamin C and antioxidant activity were obtained from Zn-2 and Zn-3 treatments, respectively. Zn-2 and Zn-3 treatments had greater zinc and phosphorus concentrations than the control treatments and Zn-sprayed fruit had greater nitrogen and potassium concentrations than the control fruit. It was concluded based on present findings that Zn treatments could be used as an effective tool to increase yield, antioxidant activity and mineral elements of ‘Granny Smith’ apples.
Keywords	antioxidant activity ; ascorbic acid ; flavonoids ; flowering ; fruit quality ; fruits ; nitrogen ; phenolic compounds ; phosphorus ; potassium ; walnuts ; zinc
Language	English
Dates of publication	2021-0101
Size	p. 670-680.
Publishing place	Taylor & Francis
Document type	Article
ZDB-ID	2235787-7
ISSN	1553-8621 ; 1553-8362
ISSN (online)	1553-8621
ISSN	1553-8362
DOI	10.1080/15538362.2021.1920554
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Article ; Online: Morphometric measurements for potential dangers of anterior intra-pelvic approach in women: A cadaveric study.

Özmeriç, Ahmet / Alemdaroğlu, Kadir Bahadır / Fırat, Ayşegül / Şahin, Özgür

Acta orthopaedica et traumatologica turcica

2023 Volume 57, Issue 4, Page(s) 183–188

Abstract: Objective: This study aimed to improve the surgical anatomical knowledge of pelvic/acetabular trauma surgeons by providing detailed morphometric data on some of the most vulnerable arteries and nerves due to constant bony landmarks during anterior intra- ...

Abstract	Objective: This study aimed to improve the surgical anatomical knowledge of pelvic/acetabular trauma surgeons by providing detailed morphometric data on some of the most vulnerable arteries and nerves due to constant bony landmarks during anterior intra-pelvic approach fixation of acetabular fractures in women. Methods: Ten hemipelvis were dissected from 5 female cadavers. The following measurements relative to the symphysis were performed: (1) the distance of the corona mortis anastomosis and (2) the bisection of the external iliac vein with the pubic ramus. In addition, dis- tance to the pelvic brim at the level of pectineal convexity of the following structures was measured: (3) depth of obturatory neurovascu- lar bundle, (4) superior vesical artery, and (5) vaginal artery. Also, the clock position of the (6) gluteal superior and inferior vessels due to sciatic notch in the supine position. Due to antero-superior corner of sacroiliac joint (7) location of the common iliac artery bifurcation, (8) location of the bifurcation of internal iliac vessels to truncuses, (9) bifurcation of superior gluteal artery and lateral sacral artery, and (10) L5 nerve were measured. The descriptive statistics were given as medians and ranges as this is a descriptive anatomical study without comparisons. Results: The median distance of corona mortis to symphysis pubis was 59.5 mm (range = 58-61). The external iliac vein bisected the pubic arm 68.5 mm (range=65-70) lateral to the symphysis pubis. At the level of pectineal convexity (about the middle of the pelvic brim), obturatory neurovascular bundle, superior vesical artery, and vaginal artery were 15 mm (range=13-16), 24 mm (range=23-25), and 36 mm (range=34-38) inferior to the pelvic brim, respectively. The superior gluteal vessels leave the sciatic notch at 12 o'clock position in supine position. Inferior gluteal vessels leave the sciatic notch at 31⁄2 o'clock position (given for left side). Common iliac artery bifurcation bisects the SI joint 5 mm (4-7) superior to antero-superior corner of the Sacro-iliac (SI) joint. The internal iliac artery gives its posterior trunk 18 mm (range=15-20) straightly anterior to antero-superior corner of the SI joint. Bifurcation of superior gluteal artery and lateral sacral artery was 11 mm (range = 10-12) away from the beginning of the posterior truncus. L5 root's medial margin was 9 mm (range = 7-10) medial to this landmark, where its lateral margin was on the SI joint (2 mm medial to 2 mm lateral). Conclusion: The majority of the bleeding complications of the major branches of the internal and external iliac arteries and neurologic palsies due to obturatory nerve and L5 nerve root damage within the operative field of the anterior intra-pelvic approach can be avoided or managed by utilizing morphometric data provided from this study. Level of evidence: N/A.
MeSH term(s)	Female ; Humans ; Pelvis ; Sacroiliac Joint ; Arteries ; Iliac Vein ; Cadaver
Language	English
Publishing date	2023-09-05
Publishing country	Turkey
Document type	Journal Article
ISSN	2589-1294
ISSN (online)	2589-1294
DOI	10.5152/j.aott.2023.23013
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Article ; Online: A long-term complication of clitoral cyst after female genital mutilation.

Şahin, Özgür / Varlı, Erol Nadi / Moallim, Abdirahman Omar / Tolunay, Harun Egemen

The Pan African medical journal

2023 Volume 46, Page(s) 23

Abstract: Female genital mutilation (FGM) was seen in 30 countries, especially in Africa and also in Asia and the Middle East. According to WHO data, Somalia is where FGM is performed most frequently. Our study aimed to evaluate the recordings of patients with FGM ...

Abstract	Female genital mutilation (FGM) was seen in 30 countries, especially in Africa and also in Asia and the Middle East. According to WHO data, Somalia is where FGM is performed most frequently. Our study aimed to evaluate the recordings of patients with FGM who were diagnosed with a traumatic clitoral cyst. We identified the clitoral cyst cases between February 2015 and August 2020. We collected clinical, surgical, sociodemographic, and histopathological details such as age, marital status, patient resume, age at which FGM was performed, complaints, size of the cyst consultation reasons, FGM procedural long-term complications, sexual function, husband polygamic relationship status, and histological findings. A total of 21 patients diagnosed with clitoral cysts were included in the study. The technique was easily applied in every patient, and the cysts were removed intact, except in 2 patients. There were no intraoperative complications; only minimal bleeding was seen. Except for one patient, all had unilocular cysts, and the final pathological examination revealed an epidermal inclusion cyst. We observed a neuroma developed due to genital trauma due to FGM in one of our patients. Female circumcision and its consequences are not familiar to many healthcare professionals in the developed world. We want to increase awareness of female circumcision and its long-term complication of clitoral cysts among healthcare professionals worldwide.
MeSH term(s)	Female ; Humans ; Circumcision, Female/adverse effects ; Epidermal Cyst/surgery ; Clitoris/pathology ; Clitoris/surgery ; Plastic Surgery Procedures ; Somalia
Language	English
Publishing date	2023-09-14
Publishing country	Uganda
Document type	Case Reports
ZDB-ID	2514347-5
ISSN	1937-8688 ; 1937-8688
ISSN (online)	1937-8688
ISSN	1937-8688
DOI	10.11604/pamj.2023.46.23.31939
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Article: Can Stria Gravidarum Predict Surgical Fluid Loss in Cesarean Section?

Arslanca, Seyma Banu / Sahin, Ozgur / Erkayıran, Ugurkan / Basarır, Zehra Ozturk / Arslanca, Tufan

Dermatology practical & conceptual

2023 Volume 13, Issue 3

Abstract: Introduction: Alterations in collagen subtypes and matrix can potentially cause fluid loss in surgery which is important in terms of liquid loss.: Objectives: The study aimed to analyze stria gravidarum (SG) and its severity in pregnant women who had ...

Abstract	Introduction: Alterations in collagen subtypes and matrix can potentially cause fluid loss in surgery which is important in terms of liquid loss. Objectives: The study aimed to analyze stria gravidarum (SG) and its severity in pregnant women who had undergone cesarean section (CS) and to evaluate surgical fluid loss (SFL) that occurred during CS operation. Methods: The research was designed as a prospective clinical cohort study to compare the amount of SFL in the second cesarean section with the severity of SG at 34-37 weeks pregnant (N 308). The severity of SG was evaluated in the preoperative period using the Davey scoring. All patients were defined none, mild stria and severe stria. The SFL was calculated by weighing the pre-and post-operative weights of the sponges. Results: The weight gain (P = 0.008) and body mass index (BMI, P = 0.017) gradually increased toward severe SG. In correlation analysis of SFL, a positive correlation was found with Davey (r=0.791; P = 0.0001), weight gained during pregnancy (r=0.328; P = 0.0001), BMI (r=0.453; P = 0.001) and newborn weight (r=0.139; P = 0.003). In the receiver operating characteristic for the predictability of SG severity on SFL, severe SG showed a potential for SFL with 95.1% specificity and 93.2% sensitivity at 791 cut-offs (area under the curve:0.987; P = 0.00001; 95% confidence interval: 0.977-0.997). Conclusions: The SG severity and SFL showed a very strong relationship, which was a very important finding that would affect the approach of the surgeons to the patients with SG in terms of fluid loss in CS.
Language	English
Publishing date	2023-07-01
Publishing country	Austria
Document type	Journal Article
ZDB-ID	2685397-8
ISSN	2160-9381
ISSN	2160-9381
DOI	10.5826/dpc.1303a175
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Article ; Online: A novel prenatal index predicting the probability of neonatal intensive care in pregnants: amnion progesterone receptor to alfa fetoprotein rate.

Arslanca, Seyma Banu / Ecemis, Tolga / Sahin, Ozgur / Ayhan, Sevgi / Arslanca, Tufan / Yucel, Gamze Sinem

Ginekologia polska

2023

Abstract: Introduction: Amniocentesis (AC) is the most used interventional procedure for prenatal diagnosis. The study aims to evaluate the pregnancy outcomes undergoing AC and the potential of amnion progesterone receptor (aPR) to alfa fetoprotein (AFP) rate for ...

Abstract	Introduction: Amniocentesis (AC) is the most used interventional procedure for prenatal diagnosis. The study aims to evaluate the pregnancy outcomes undergoing AC and the potential of amnion progesterone receptor (aPR) to alfa fetoprotein (AFP) rate for predicting the probability of neonatal intensive care unit (NICU). Material and methods: This prospective cross-sectional study population consisted of 85 pregnant women who underwent mid-trimester AC. All cases were screened by ultrasound before AC. Maternal venous and amniotic samples were obtained simultaneously to evaluate the serum progesterone (sPRG), aPR, and aAFP and analyzed with patient results. Results: Unlike sPRG and aAFP, aPR showed a positive correlation with NICU and a negative correlation with parity. In linear regression, the aPR-AFP rate showed strong linearity with NICU and parity. In an aPR-AFP rate analysis, we saw a strong predictivity for NICU compared to the other three parameters. It presented 73.4% specificity and 79% sensitivity at 0.0075 cut-off (AUC: 0.78; p = 0.003; 95% CI: 0.608-0.914). Conclusions: Evaluating the PR either alone or in a rational combination with AFP will provide physicians with valuable information about the advanced process of pregnancy and postpartum complications. The physicians might use the aPR-AFP rate to predict NICU potential for pregnancy and need further studies to make more vital predictions on postpartum complications.
Language	English
Publishing date	2023-05-10
Publishing country	Poland
Document type	Journal Article
ZDB-ID	130894-4
ISSN	2543-6767 ; 0017-0011
ISSN (online)	2543-6767
ISSN	0017-0011
DOI	10.5603/GP.a2023.0046
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Article ; Online: Endocrine resistance in breast cancer: from molecular mechanisms to therapeutic strategies.

Saatci, Ozge / Huynh-Dam, Kim-Tuyen / Sahin, Ozgur

Journal of molecular medicine (Berlin, Germany)

2021 Volume 99, Issue 12, Page(s) 1691–1710

Abstract: Estrogen receptor-positive (ER +) breast cancer accounts for approximately 75% of all breast cancers. Endocrine therapies, including selective ER modulators (SERMs), aromatase inhibitors (AIs), and selective ER down-regulators (SERDs) provide substantial ...

Abstract	Estrogen receptor-positive (ER +) breast cancer accounts for approximately 75% of all breast cancers. Endocrine therapies, including selective ER modulators (SERMs), aromatase inhibitors (AIs), and selective ER down-regulators (SERDs) provide substantial clinical benefit by reducing the risk of disease recurrence and mortality. However, resistance to endocrine therapies represents a major challenge, limiting the success of ER + breast cancer treatment. Mechanisms of endocrine resistance involve alterations in ER signaling via modulation of ER (e.g., ER downregulation, ESR1 mutations or fusions); alterations in ER coactivators/corepressors, transcription factors (TFs), nuclear receptors and epigenetic modulators; regulation of signaling pathways; modulation of cell cycle regulators; stress signaling; and alterations in tumor microenvironment, nutrient stress, and metabolic regulation. Current therapeutic strategies to improve outcome of endocrine-resistant patients in clinics include inhibitors against mechanistic target of rapamycin (mTOR), cyclin-dependent kinase (CDK) 4/6, and the phosphoinositide 3-kinase (PI3K) subunit, p110α. Preclinical studies reveal novel therapeutic targets, some of which are currently tested in clinical trials as single agents or in combination with endocrine therapies, such as ER partial agonists, ER proteolysis targeting chimeras (PROTACs), next-generation SERDs, AKT inhibitors, epidermal growth factor receptor 1 and 2 (EGFR/HER2) dual inhibitors, HER2 targeting antibody-drug conjugates (ADCs) and histone deacetylase (HDAC) inhibitors. In this review, we summarize the established and emerging mechanisms of endocrine resistance, alterations during metastatic recurrence, and discuss the approved therapies and ongoing clinical trials testing the combination of novel targeted therapies with endocrine therapy in endocrine-resistant ER + breast cancer patients.
MeSH term(s)	Animals ; Antineoplastic Agents/therapeutic use ; Aromatase Inhibitors/therapeutic use ; Breast Neoplasms/drug therapy ; Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Drug Resistance, Neoplasm/genetics ; Estrogen Receptor Modulators/therapeutic use ; Female ; Humans ; Neoplasm Recurrence, Local/genetics ; Receptors, Estrogen/genetics ; Receptors, Estrogen/metabolism
Chemical Substances	Antineoplastic Agents ; Aromatase Inhibitors ; Estrogen Receptor Modulators ; Receptors, Estrogen
Language	English
Publishing date	2021-10-08
Publishing country	Germany
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1223802-8
ISSN	1432-1440 ; 0946-2716
ISSN (online)	1432-1440
ISSN	0946-2716
DOI	10.1007/s00109-021-02136-5
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Article ; Online: Transcriptome analysis of polyploid giant cancer cells and their progeny reveals a functional role for p21 in polyploidization and depolyploidization.

White-Gilbertson, Shai / Lu, Ping / Saatci, Ozge / Sahin, Ozgur / Delaney, Joe R / Ogretmen, Besim / Voelkel-Johnson, Christina

The Journal of biological chemistry

2024 Volume 300, Issue 4, Page(s) 107136

Abstract: Polyploid giant cancer cells (PGCC) are frequently detected in tumors and are increasingly recognized for their roles in chromosomal instability and associated genome evolution that leads to cancer recurrence. We previously reported that therapy stress ... ...

Abstract	Polyploid giant cancer cells (PGCC) are frequently detected in tumors and are increasingly recognized for their roles in chromosomal instability and associated genome evolution that leads to cancer recurrence. We previously reported that therapy stress promotes polyploidy, and that acid ceramidase plays a role in depolyploidization. In this study, we used an RNA-seq approach to gain a better understanding of the underlying transcriptomic changes that occur as cancer cells progress through polyploidization and depolyploidization. Our results revealed gene signatures that are associated with disease-free and/or overall survival in several cancers and identified the cell cycle inhibitor CDKN1A/p21 as the major hub in PGCC and early progeny. Increased expression of p21 in PGCC was limited to the cytoplasm. We previously demonstrated that the sphingolipid enzyme acid ceramidase is dispensable for polyploidization upon therapy stress but plays a crucial role in depolyploidization. The current study demonstrates that treatment of cells with ceramide is not sufficient for p53-independent induction of p21 and that knockdown of acid ceramidase, which hydrolyzes ceramide, does not interfere with upregulation of p21. In contrast, blocking the expression of p21 with UC2288 prevented the induction of acid ceramidase and inhibited both the formation of PGCC from parental cells as well as the generation of progeny from PGCC. Taken together, our data suggest that p21 functions upstream of acid ceramidase and plays an important role in polyploidization and depolyploidization.
MeSH term(s)	Polyploidy ; Humans ; Cyclin-Dependent Kinase Inhibitor p21/metabolism ; Cyclin-Dependent Kinase Inhibitor p21/genetics ; Neoplasms/genetics ; Neoplasms/metabolism ; Neoplasms/pathology ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Gene Expression Profiling ; Transcriptome ; Giant Cells/metabolism
Chemical Substances	Cyclin-Dependent Kinase Inhibitor p21 ; CDKN1A protein, human
Language	English
Publishing date	2024-03-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1016/j.jbc.2024.107136
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Article ; Online: Calibration of T-shaped atomic force microscope cantilevers using the thermal noise method.

Kim, Youngkyu / Mandriota, Nicola / Goodnight, Davis / Sahin, Ozgur

The Review of scientific instruments

2020 Volume 91, Issue 8, Page(s) 83703

Abstract: The tip-sample interaction force measurements in atomic force microscopy (AFM) provide information about materials' properties with nanoscale resolution. The T-shaped cantilevers used in Torsional-Harmonic AFM allow measuring the rapidly changing tip- ... ...

Abstract	The tip-sample interaction force measurements in atomic force microscopy (AFM) provide information about materials' properties with nanoscale resolution. The T-shaped cantilevers used in Torsional-Harmonic AFM allow measuring the rapidly changing tip-sample interaction forces using the torsional (twisting) deflections of the cantilever due to the off-axis placement of the sharp tip. However, it has been difficult to calibrate these cantilevers using the commonly used thermal noise-based calibration method as the mechanical coupling between flexural and torsional deflections makes it challenging to determine the deflection sensitivities from force-distance curves. Here, we present thermal noise-based calibration of these T-shaped AFM cantilevers by simultaneously analyzing flexural and torsional thermal noise spectra, along with deflection signals during a force-distance curve measurement. The calibration steps remain identical to the conventional thermal noise method, but a computer performs additional calculations to account for mode coupling. We demonstrate the robustness of the calibration method by determining the sensitivity of calibration results to the laser spot position on the cantilever, to the orientation of the cantilever in the cantilever holder, and by repeated measurements. We validated the quantitative force measurements against the known unfolding force of a protein, the I91 domain of titin, which resulted in consistent unfolding force values among six independently calibrated cantilevers.
Language	English
Publishing date	2020-08-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	209865-9
ISSN	1089-7623 ; 0034-6748
ISSN (online)	1089-7623
ISSN	0034-6748
DOI	10.1063/5.0013091
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