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  1. AU="Saito, Kazuyuki"
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  1. Article ; Online: Effect existence of aging on stutter ratio evaluated via Bayesian inference.

    Inokuchi, Shota / Nakanishi, Hiroaki / Takada, Aya / Saito, Kazuyuki

    Forensic science international. Genetics

    2023  Volume 67, Page(s) 102933

    Abstract: The stochastic behavior of the stutter ratio (SR) in capillary electrophoresis-based DNA typing is currently described and predicted using statistical models in forensic genetics. Clarifying this behavior can help obtain more objective and robust ... ...

    Abstract The stochastic behavior of the stutter ratio (SR) in capillary electrophoresis-based DNA typing is currently described and predicted using statistical models in forensic genetics. Clarifying this behavior can help obtain more objective and robust evidence to the court in terms of mixture interpretation. This study aimed to investigate the effect existence of aging on SR via a Bayesian framework. Nail scrapings and clippings were collected from 68 healthy individuals with informed consent. Samples were classified by age-class: young group (0-16 years; n = 36) and older-adult group (>61 years; n = 32). Then, they were compared in terms of their SRs for each simple repeat locus included in GlobalFiler Kit. Bayesian modeling was performed with lognormal distribution model, which implemented multiple linear regression, allele and age-class as explanatory variables. For all simple repeat loci, the median of the posterior distribution of the age-class parameter was a positive value. For CSF1PO and D7S820, the 95% credible interval of the posterior distribution did not include 0. Our data suggested that aging slightly increases the SR. These findings might help elucidate the stochastic behavior of SR.
    MeSH term(s) Humans ; Infant, Newborn ; Infant ; Child, Preschool ; Child ; Adolescent ; Microsatellite Repeats ; Bayes Theorem ; DNA Fingerprinting ; Alleles ; Aging
    Language English
    Publishing date 2023-09-09
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2493339-9
    ISSN 1878-0326 ; 1872-4973
    ISSN (online) 1878-0326
    ISSN 1872-4973
    DOI 10.1016/j.fsigen.2023.102933
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  2. Article ; Online: Estimating bloodstain age in the short term based on DNA fragment length using nanopore sequencer.

    Nakanishi, Hiroaki / Takada, Aya / Yoneyama, Katsumi / Hara, Masaaki / Sakai, Kentaro / Saito, Kazuyuki

    Forensic science international

    2024  Volume 358, Page(s) 112010

    Abstract: We used a nanopore sequencer to quantify DNA fragments > 10,000 bp in size and then evaluated their relationship with short-term bloodstain age. Moreover, DNA degradation was investigated after bloodstains were wetted once with water. Bloodstain samples ... ...

    Abstract We used a nanopore sequencer to quantify DNA fragments > 10,000 bp in size and then evaluated their relationship with short-term bloodstain age. Moreover, DNA degradation was investigated after bloodstains were wetted once with water. Bloodstain samples on cotton gauze were stored at room temperature and low humidity for up to 6 months. Bloodstains stored for 1 day were wetted with nuclease-free water, allowed to dry, and stored at room temperature and low humidity for up to 1 week. The proportion of fragments > 20,000 bp in dry bloodstains tended to decrease over time, particularly for fragments > 50,000 bp in size. This trend was modeled using a power approximation curve, with the highest R
    MeSH term(s) Blood Stains ; Humans ; DNA ; Specimen Handling/instrumentation ; Specimen Handling/methods ; Nanopores ; Sequence Analysis, DNA ; DNA Degradation, Necrotic ; Time Factors ; DNA Fragmentation ; DNA Fingerprinting/instrumentation ; DNA Fingerprinting/methods
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2024-03-27
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 424042-x
    ISSN 1872-6283 ; 0379-0738
    ISSN (online) 1872-6283
    ISSN 0379-0738
    DOI 10.1016/j.forsciint.2024.112010
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  3. Article ; Online: Simplified detection of the species of origin of antler velvets using single-stranded tag hybridization chromatographic printed-array strip.

    Nakanishi, Hiroaki / Takada, Aya / Yoneyama, Katsumi / Sakai, Kentaro / Saito, Kazuyuki

    Journal of natural medicines

    2023  Volume 77, Issue 4, Page(s) 998–1004

    Abstract: In this study, we developed a convenient and easy-to-use origin identification method for antler velvets based on a simple DNA extraction technique and single-stranded tag hybridization chromatographic printed-array strip (STH-PAS). The primer sets used ... ...

    Abstract In this study, we developed a convenient and easy-to-use origin identification method for antler velvets based on a simple DNA extraction technique and single-stranded tag hybridization chromatographic printed-array strip (STH-PAS). The primer sets used to detect Cervus elaphus, Rangifer tarandus, and 12S rRNA did not engage in non-specific reactions such as primer dimer formation. In both the triplex and singleplex assays, the sensitivity was < 1 ng DNA. Moreover, Cervus elaphus DNA could be detected in OTC crude drug products. Although the detection sensitivity resulting from the simplified extraction was slightly lower than that obtained with extraction by conventional methods, the amount of DNA was sufficient even from a small sample. The choice of a triplex or singleplex assay will depend on the purpose of the test. For example, if it is important to determine whether the antler velvet is derived from Cervus elaphus or Rangifer tarandus, a triplex assay is appropriate. If it is necessary to explore whether antler velvet from Cervus elaphus is included in an OTC crude drug product, a singleplex assay using the Cervus elaphus primer set is informative. If it is necessary to explore whether powdered antler velvet includes counterfeit products (from Rangifer tarandus), a singleplex assay employing the Rangifer tarandus primer is appropriate. The singleplex assay detects minor components even at a 1,000:1 ratio. Our study thus demonstrated the utility of a method combining simple DNA extraction with STH-PAS for efficient identification of the origin of antler velvets.
    MeSH term(s) Animals ; Reindeer ; Antlers/chemistry ; Deer/genetics
    Language English
    Publishing date 2023-08-14
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2227540-X
    ISSN 1861-0293 ; 1340-3443
    ISSN (online) 1861-0293
    ISSN 1340-3443
    DOI 10.1007/s11418-023-01734-6
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    Zs.A 2692: Show issues Location:
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  4. Article ; Online: Autopsy findings of post-COVID-19 vaccination deaths in Tokyo Metropolis, Japan, 2021.

    Suzuki, Hideto / Ro, Ayako / Takada, Aya / Saito, Kazuyuki / Hayashi, Kino

    Legal medicine (Tokyo, Japan)

    2022  Volume 59, Page(s) 102134

    Abstract: Background: COVID-19 vaccines have been used across Japan since 17 February 2021, and as of 17 April 2022, 1690 deaths potentially caused by vaccine-related adverse effects have been reported to the Ministry of Health, Labour and Welfare. However, the ... ...

    Abstract Background: COVID-19 vaccines have been used across Japan since 17 February 2021, and as of 17 April 2022, 1690 deaths potentially caused by vaccine-related adverse effects have been reported to the Ministry of Health, Labour and Welfare. However, the causal relationship between vaccination and death could not be fully evaluated because of a lack of sufficient information.
    Methods: Autopsy cases in which deaths occurred within seven days after COVID-19 vaccination in Tokyo Metropolis and were handled by medical examiners were selected (n = 54). Age, sex, vaccine-related information, cause of death, and possible causal relationship between vaccination and death were examined.
    Results: The mean age of the deceased individuals was 68.1 years, and the study sample consisted of 34 males (63.9%) and 20 females (37.0%). Thirty-seven and six individuals received Comirnaty and Spikevax, respectively (68.5% and 11.1% respectively). The manner of death included natural (n = 43), non-natural (n = 8), and undetermined (n = 3). The most frequent cause of death was ischemic heart disease (n = 16). Regarding causal relationships, 46 cases (85.2%) did not show a causal relationship to vaccination, except for myocarditis (n = 3), thrombosis-related death (n = 4), and others (n = 1).
    Conclusion: Although many cases of deaths after COVID-19 vaccination in this study showed no definite causal relationship between the vaccination and deaths, some cases showed possible adverse events such as myocarditis. Autopsies are essential for detecting vaccine-related deaths, and the Japanese death investigation system needs to be reinforced from this viewpoint.
    MeSH term(s) Male ; Female ; Humans ; Aged ; Autopsy ; COVID-19 Vaccines/adverse effects ; Japan/epidemiology ; Tokyo/epidemiology ; COVID-19/prevention & control ; Myocarditis ; Vaccination
    Chemical Substances COVID-19 Vaccines
    Language English
    Publishing date 2022-08-20
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 2019555-2
    ISSN 1873-4162 ; 1344-6223
    ISSN (online) 1873-4162
    ISSN 1344-6223
    DOI 10.1016/j.legalmed.2022.102134
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  5. Article ; Online: Unexpected death in a young child associated with anomalous aortic origin of the left main coronary artery without physical exertion: A case of an anomalous coronary artery with highly abundant elastic fibers.

    Sakai, Kentaro / Saito, Kazuyuki / Takada, Aya / Suzuki, Hideto

    Legal medicine (Tokyo, Japan)

    2021  Volume 53, Page(s) 101965

    Abstract: Sudden death due to anomalous aortic origin of a coronary artery is far less common among young children in the absence of exercise stress. This report describes the case of a 2-year-old boy with a lower respiratory tract infection who suffered sudden ... ...

    Abstract Sudden death due to anomalous aortic origin of a coronary artery is far less common among young children in the absence of exercise stress. This report describes the case of a 2-year-old boy with a lower respiratory tract infection who suffered sudden cardiac arrest in his bed at home. The autopsy revealed that the left coronary artery (LCA) originated from the right sinus of Valsalva with an acute angle takeoff and traveled between the aorta and the pulmonary trunk (an interarterial course). Upon histological examination, the LCA, before reaching its major branches, was located adjacent to the outside of the aortic wall without an intramural passage, and the arterial wall was composed almost exclusively of elastic fibers without media containing smooth muscle cells throughout the entire length of the abnormal running. Screening tests for respiratory virus infection detected enterovirus in the lung tissue. In association with an acute angle takeoff and interarterial course, the wall structure with highly abundant elastic fibers that are more flexible tissues among blood vessel components might suggest their vulnerability to compression during the great vessels' systolic expansion in the sympathetic activation induced by the viral infection, leading to fatal myocardial ischemia without physical exertion.
    MeSH term(s) Aorta ; Child, Preschool ; Coronary Vessel Anomalies ; Death, Sudden, Cardiac/etiology ; Elastic Tissue ; Humans ; Male ; Physical Exertion
    Language English
    Publishing date 2021-09-15
    Publishing country Ireland
    Document type Case Reports ; Journal Article
    ZDB-ID 2019555-2
    ISSN 1873-4162 ; 1344-6223
    ISSN (online) 1873-4162
    ISSN 1344-6223
    DOI 10.1016/j.legalmed.2021.101965
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  6. Article ; Online: Development of an Okinawa panel for biogeographic inference of Okinawans.

    Nakanishi, Hiroaki / Pereira, Vania / Børsting, Claus / Tvedebrink, Torben / Takada, Aya / Saito, Kazuyuki

    Annals of human biology

    2023  Volume 50, Issue 1, Page(s) 436–441

    Abstract: Background: The Precision ID Ancestry Panel with 165 SNP markers was unable to differentiate between mainland Japanese and Okinawa Japanese or to distinguish either of them from other East Asian populations.: Aim: An Okinawa panel was developed with ... ...

    Abstract Background: The Precision ID Ancestry Panel with 165 SNP markers was unable to differentiate between mainland Japanese and Okinawa Japanese or to distinguish either of them from other East Asian populations.
    Aim: An Okinawa panel was developed with the aim of further separating Okinawa Japanese individuals from mainland Japanese and other Asian groups. Seventy-five SNPs were selected using the most informative markers from the literature. Further, 22 SNPs were selected to separate Okinawa Japanese at minimum SNPs.
    Subjects and methods: Samples were collected from 48 unrelated individuals from mainland Japan and 46 unrelated residents of the Okinawa prefecture. Data were evaluated by STRUCTURE, principal component, and GenoGeographer analyses.
    Results: The 22 SNP set had similar levels of differentiation in STRUCTURE and PCA analyses as the 75 SNP set. GenoGeographer analysis showed that, out of the 46 Okinawa Japanese individuals, the 75 SNP and 22 SNP sets correctly assigned the Okinawan population as the most likely population of origin for 32 and 31 individuals, respectively.
    Conclusion: Neither SNP set could completely differentiate between Okinawa Japanese and other Asian groups, however, these sets should be useful for crime investigation, when the sample, cost and time are limited.
    MeSH term(s) Humans ; Asian People/genetics ; Genetics, Population ; Polymorphism, Single Nucleotide ; East Asian People ; Japan ; Genotype ; Gene Frequency
    Language English
    Publishing date 2023-10-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 186656-4
    ISSN 1464-5033 ; 0301-4460
    ISSN (online) 1464-5033
    ISSN 0301-4460
    DOI 10.1080/03014460.2023.2257594
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  7. Article ; Online: Dissecting lesions in a culprit artery of a hemorrhagic focus in the basal ganglia: Histopathological analysis by serial sectioning.

    Takada, Aya / Saito, Kazuyuki / Sakai, Kentaro

    Neuropathology : official journal of the Japanese Society of Neuropathology

    2021  Volume 41, Issue 4, Page(s) 301–305

    Abstract: In a hypertensive hemorrhagic focus of the basal ganglia, the culprit arteries have been reported to be associated with dissecting lesions, whose topographical relationship to the rupture sites remains to be clarified. Herein we describe multiple ... ...

    Abstract In a hypertensive hemorrhagic focus of the basal ganglia, the culprit arteries have been reported to be associated with dissecting lesions, whose topographical relationship to the rupture sites remains to be clarified. Herein we describe multiple dissecting lesions in the culprit artery of hypertensive hemorrhage of the basal ganglia. A 1.0 × 0.8 × 0.8 cm-sized bleeding globe was confirmed at a left lenticulostriate artery and histologically analyzed by serial sectioning. Three independent dissecting lesions were identified in the culprit artery. They were situated near the bifurcations, ranging from 240 to 3200 μm in length. The dissections mainly occurred between the intima and media with disruption of the internal elastic lamina (IEL), forming a fresh thrombus within the false lumen. Two rupture sites causing the cerebral hematoma were confirmed away from the dissecting lesions. One was situated close but not adjacent to the longest dissecting lesion; the other, measuring approximately 150 μm in diameter, was adjacent to the bifurcation of an artery. The histopathological findings suggest that the dissecting lesion resulted from medial detachment following IEL disruption in the process of arterial rupture of the culprit artery. We conclude that this was a secondary manifestation during the rupture rather than a cause of the arterial rupture.
    MeSH term(s) Arteries ; Basal Ganglia ; Cerebral Hemorrhage ; Humans ; Hypertension
    Language English
    Publishing date 2021-03-21
    Publishing country Australia
    Document type Case Reports
    ZDB-ID 1483794-8
    ISSN 1440-1789 ; 0919-6544
    ISSN (online) 1440-1789
    ISSN 0919-6544
    DOI 10.1111/neup.12736
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    Zs.A 5086: Show issues Location:
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  8. Article: Characteristics of a Surgical Snare Using Microwave Energy.

    Sugiyama, Masashi / Saito, Kazuyuki

    Diagnostics (Basel, Switzerland)

    2018  Volume 8, Issue 4

    Abstract: Currently, minimally invasive treatments that insert various treatment devices into an endoscope are actively being performed. A high-frequency (HF) snare is commonly used as an energy device inserted into an endoscope. However, using a high-frequency ... ...

    Abstract Currently, minimally invasive treatments that insert various treatment devices into an endoscope are actively being performed. A high-frequency (HF) snare is commonly used as an energy device inserted into an endoscope. However, using a high-frequency snare, problems usually occur, such as the obstruction of the visual field caused by smoke. On the other hand, microwave heating produces less smoke and provides a better visual field. In this study, a snare using microwave energy inserted into an endoscope is proposed, and its characteristics are evaluated.
    Language English
    Publishing date 2018-12-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics8040083
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  9. Article ; Online: Long-term outcomes and safety of radical transmediastinal esophagectomy with preoperative docetaxel, cisplatin, and 5-fluorouracil combination chemotherapy for locally advanced squamous cell carcinoma of the thoracic esophagus.

    Yamagata, Yukinori / Saito, Kazuyuki / Hirano, Kosuke / Oya, Masatoshi

    World journal of surgical oncology

    2020  Volume 18, Issue 1, Page(s) 252

    Abstract: Background: It is unknown whether transmediastinal esophagectomy (TME) is an acceptable surgical procedure for locally advanced esophageal squamous cell carcinoma (ESCC). Therefore, we investigated the feasibility of long-term survival after TME with ... ...

    Abstract Background: It is unknown whether transmediastinal esophagectomy (TME) is an acceptable surgical procedure for locally advanced esophageal squamous cell carcinoma (ESCC). Therefore, we investigated the feasibility of long-term survival after TME with neoadjuvant docetaxel, cisplatin, and 5-fluorouracil combination chemotherapy (DCF therapy).
    Methods: This retrospective, observational study included locally advanced resectable ESCC. All patients received two cycles of preoperative DCF therapy (60 mg/m
    Results: Sixteen patients were included in this study. All patients received two cycles of DCF therapy, followed by surgery. The median follow-up duration of the 16 patients was 35.4 months. The 2-year overall survival (OS) was 93.3% (95% confidence interval [CI], 61.3-99.0), and the 3-year OS was 78.8% (95% CI, 47.3-92.7). The 2-year and 3-year relapse-free survivals were both 73.3% (95% CI, 43.6-89.1). Leukopenia and neutropenia occurred in most patients; however, they were controllable. Fifteen patients completed TME, and one was converted to open transthoracic esophagectomy because of tracheal injury. Three-field dissection was performed for 12 of 16 patients (75%), and R0 resection was achieved in 15 of 16 patients (93.8%). Three cases of grade IIIb chylothorax were observed. There was no mortality in this study.
    Conclusion: Combined neoadjuvant DCF and TME for locally advanced ESCC was safe and less invasive than traditional therapies and had a satisfactory long-term prognosis.
    MeSH term(s) Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Carcinoma, Squamous Cell/drug therapy ; Carcinoma, Squamous Cell/surgery ; Cisplatin/therapeutic use ; Docetaxel ; Esophageal Neoplasms/drug therapy ; Esophageal Neoplasms/surgery ; Esophageal Squamous Cell Carcinoma ; Esophagectomy ; Fluorouracil/therapeutic use ; Humans ; Neoadjuvant Therapy ; Neoplasm Recurrence, Local ; Prognosis ; Retrospective Studies
    Chemical Substances Docetaxel (15H5577CQD) ; Cisplatin (Q20Q21Q62J) ; Fluorouracil (U3P01618RT)
    Language English
    Publishing date 2020-09-22
    Publishing country England
    Document type Journal Article ; Observational Study
    ZDB-ID 2118383-1
    ISSN 1477-7819 ; 1477-7819
    ISSN (online) 1477-7819
    ISSN 1477-7819
    DOI 10.1186/s12957-020-02023-2
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  10. Article ; Online: Estimating included animal species in mixed crude drugs derived from animals using massively parallel sequencing.

    Nakanishi, Hiroaki / Yoneyama, Katsumi / Hara, Masaaki / Takada, Aya / Saito, Kazuyuki

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 6257

    Abstract: We developed a method that can detect each animal species of origin for crude drugs derived from multiple animal species based on massively parallel sequencing analysis of mitochondrial genes. The crude drugs derived from animals investigated in this ... ...

    Abstract We developed a method that can detect each animal species of origin for crude drugs derived from multiple animal species based on massively parallel sequencing analysis of mitochondrial genes. The crude drugs derived from animals investigated in this study were Cervi Parvum Cornu and Trogopterorum feces, which are derived from a mix of different animal species, two chopped cicada sloughs, and two commercial Kampo drugs. The mitochondrial 12S rRNA, 16S rRNA, and cytochrome oxidase subunit I gene regions were amplified and sequenced using MiSeq. The ratios of haplotype to total number of sequences reads were calculated after sequence extraction and trimming. Haplotypes that exceeded the threshold were defined as positive haplotypes, which were compared with all available sequences using BLAST. In the Cervi Parvum Cornu and Trogopterorum feces samples, the haplotype ratios corresponded roughly to the mixture ratios, although there was a slight difference from mixture ratios depending on the gene examined. This method could also roughly estimate the compositions of chopped cicada sloughs and Kampo drugs. This analysis, whereby the sequences of several genes are elucidated, is better for identifying the included animal species. This method should be useful for quality control of crude drugs and Kampo drugs.
    MeSH term(s) Animals ; Biological Products/analysis ; DNA, Mitochondrial/genetics ; Drugs, Chinese Herbal/analysis ; Electron Transport Complex IV/genetics ; Feces/chemistry ; Genes, Mitochondrial ; Guinea Pigs/genetics ; Haplotypes ; Hemiptera/chemistry ; Hemiptera/genetics ; High-Throughput Nucleotide Sequencing/methods ; Medicine, Kampo ; RNA, Ribosomal/genetics ; RNA, Ribosomal, 16S/genetics ; Real-Time Polymerase Chain Reaction/methods ; Ruminants/genetics ; Sciuridae/genetics ; Sequence Analysis, DNA/methods
    Chemical Substances Biological Products ; DNA, Mitochondrial ; Drugs, Chinese Herbal ; RNA, Ribosomal ; RNA, Ribosomal, 16S ; RNA, ribosomal, 12S ; Electron Transport Complex IV (EC 1.9.3.1)
    Language English
    Publishing date 2021-03-18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-85803-4
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